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The map label for this gene is pcl [H]

Identifier: 73537766

GI number: 73537766

Start: 564537

End: 566249

Strand: Reverse

Name: pcl [H]

Synonym: Reut_B3932

Alternate gene names: 73537766

Gene position: 566249-564537 (Counterclockwise)

Preceding gene: 73537767

Following gene: 73537765

Centisome position: 20.77

GC content: 69.99

Gene sequence:

>1713_bases
ATGACGAGCACGTTCGCCCTTCTCTCTCATCCCGACCCAGACAGCGTCGTCGCGTGGCGCGGCAGCCAACCGGTCACGGC
CAGCCGGTTCCTGGCGGATGTCCACGCGCTCGCGGCACGCCTGCCACCCGGCGACCACGTCTTCAATGCGTGTGCGGATC
GATACCGCTTTGCGGTCGGGCTCTGCGCGTCGCTGCTGTGCGGCAAGGTGAGCCTGCTGCCGTCGGCACAGACGCCGGAA
ACGGTGCGGCAACTCGCCGCGTTTGCGCCGGATGTCTTCTGCCTGTACGACGACGCGGATGGCAGCGTGGCGCTGCCCCG
TTTCTTTTACGATGCATCTGAGCCCGAGCCATCCGCACCGCTGCCCGTACCGGACATTCCGGCAGAGCGGCTGATGGCCT
ATGTGTTCACGTCGGGCACCACCGGCCAGCCTGTCGCCCATGCCAAGACGTGGGGCGCGATGGTGCGCGGCGCGCGTGCC
ACGGCGCAACGCCTTGGCCTGGCGGACGGCCGCGCGTGGACGCTGGTCGGCACGCTGCCCCCGCAGCATATGTTCGGACT
GGAAGCCACGGTGATGCTGGCCCTGCAAGGTAGCGCAGCGCTGGCCTCGGTGCGCGCGTTCTATCCGGCCGATATCCGCG
CTGCCCTGCGCGACGTCCCGCAGCCACGCGCGCTGGTGACGTCCCCGGTGCACCTGCGCGCACTGCTGCAATCAGACAGC
GACCTGCCTGCCGCCGACCTCGTGCTGTCCGCTACGGCCCCGATGAGCCGAGCCATGGCACGGCAGGCGCAGGCTGGTCT
GGCGGCACCTTTGCTCGAGATCTACGGCAGCACCGAGACCGGCGCGATGGCGACGCGCAAAACCGCCGACACGGACGAAT
GGACGCTGTTGCCCGGCATCACCCTGGAAAGCCGCAGCGATCACGCTGGCGATGGCGAATCCGAGCCCGCGATACGGGTC
CGCGGCGGCCACATCGACGAGCCAGTGGCGCTCGGCGATGCCATCGCGCCGCTCGACGCCGGGCGCTTCGTGCTCCACGG
CCGCAAGGCCGACATGATCAAGATAGCCGGCAAGCGCACGTCGCTCGCCTATCTCAATCAGCAACTCACAGCCATCGACG
GCGTGCAGGACGGCACCTTCTACGTGCCCGATGACGTGCATGAAGTCGAGGCCGCAGGCCGCGTGGTGCGGCTCGTGGCG
CTGGCCGTAGCCCCCGGCGTGCCGGCCGCGCGCATCCTGCAGGAGCTGCGCGAGCGCATCGACGCCGCGTTCCTGCCGCG
GCCGCTTTGTCTCGTCGATCGCCTGCCGCGCAATGCCGCGGGCAAGCTTCCGCGAGAAGCACTGGCGCCGCTGGTGGCCG
CGCAACTGGCACAGCGCCACGCGCCAGGCTTTGTGATCGACGCGGGGCACCCGGCGATAGCGGGCCATTTTCCGGGCAAT
CCCGTGGTCCCCGGCGTGGTACTGCTGGACCATGCGATCCTGCAGATCGGCGCAGCGCTCGGACAACCGCTTGCCGTCGC
GCAAGTTGGCACGGTCAAGTTCCTGAGCCCGGTCCGGCCCGGCGAGCGGGTCGAGGTCGCACACCAGCTGCATGGGGACC
ACGCCGGCACGGCGTCCATCCGCTTCACGCTGCGCGCGGGCGGCCGTGACGTGGCCAGCGGCACGCTGAGCACGCGGGCC
ACGCTGGCGAGCAGGGAGGCATCGCCATGCTGA

Upstream 100 bases:

>100_bases
GCCAAGGACACCGGCGTGCTCGATGCCGTGCGCGGCTATCGCACCGTGATGACCGAGCGCGCCAGCCGCGCCGGCGCGTC
GCGCTGAACGCGGATTGCTT

Downstream 100 bases:

>100_bases
CCTGGCTTCGCAAGCCCCAGCCACTGGACACCGACTGGTCCAGCCGCCAGGAACGCAGCAATGTCACGCTGATGCGCGTG
ATGACGTGGATTTCGCTCGC

Product: AMP-dependent synthetase and ligase

Products: NA

Alternate protein names: 4CL; 4-coumaroyl-CoA synthase [H]

Number of amino acids: Translated: 570; Mature: 569

Protein sequence:

>570_residues
MTSTFALLSHPDPDSVVAWRGSQPVTASRFLADVHALAARLPPGDHVFNACADRYRFAVGLCASLLCGKVSLLPSAQTPE
TVRQLAAFAPDVFCLYDDADGSVALPRFFYDASEPEPSAPLPVPDIPAERLMAYVFTSGTTGQPVAHAKTWGAMVRGARA
TAQRLGLADGRAWTLVGTLPPQHMFGLEATVMLALQGSAALASVRAFYPADIRAALRDVPQPRALVTSPVHLRALLQSDS
DLPAADLVLSATAPMSRAMARQAQAGLAAPLLEIYGSTETGAMATRKTADTDEWTLLPGITLESRSDHAGDGESEPAIRV
RGGHIDEPVALGDAIAPLDAGRFVLHGRKADMIKIAGKRTSLAYLNQQLTAIDGVQDGTFYVPDDVHEVEAAGRVVRLVA
LAVAPGVPAARILQELRERIDAAFLPRPLCLVDRLPRNAAGKLPREALAPLVAAQLAQRHAPGFVIDAGHPAIAGHFPGN
PVVPGVVLLDHAILQIGAALGQPLAVAQVGTVKFLSPVRPGERVEVAHQLHGDHAGTASIRFTLRAGGRDVASGTLSTRA
TLASREASPC

Sequences:

>Translated_570_residues
MTSTFALLSHPDPDSVVAWRGSQPVTASRFLADVHALAARLPPGDHVFNACADRYRFAVGLCASLLCGKVSLLPSAQTPE
TVRQLAAFAPDVFCLYDDADGSVALPRFFYDASEPEPSAPLPVPDIPAERLMAYVFTSGTTGQPVAHAKTWGAMVRGARA
TAQRLGLADGRAWTLVGTLPPQHMFGLEATVMLALQGSAALASVRAFYPADIRAALRDVPQPRALVTSPVHLRALLQSDS
DLPAADLVLSATAPMSRAMARQAQAGLAAPLLEIYGSTETGAMATRKTADTDEWTLLPGITLESRSDHAGDGESEPAIRV
RGGHIDEPVALGDAIAPLDAGRFVLHGRKADMIKIAGKRTSLAYLNQQLTAIDGVQDGTFYVPDDVHEVEAAGRVVRLVA
LAVAPGVPAARILQELRERIDAAFLPRPLCLVDRLPRNAAGKLPREALAPLVAAQLAQRHAPGFVIDAGHPAIAGHFPGN
PVVPGVVLLDHAILQIGAALGQPLAVAQVGTVKFLSPVRPGERVEVAHQLHGDHAGTASIRFTLRAGGRDVASGTLSTRA
TLASREASPC
>Mature_569_residues
TSTFALLSHPDPDSVVAWRGSQPVTASRFLADVHALAARLPPGDHVFNACADRYRFAVGLCASLLCGKVSLLPSAQTPET
VRQLAAFAPDVFCLYDDADGSVALPRFFYDASEPEPSAPLPVPDIPAERLMAYVFTSGTTGQPVAHAKTWGAMVRGARAT
AQRLGLADGRAWTLVGTLPPQHMFGLEATVMLALQGSAALASVRAFYPADIRAALRDVPQPRALVTSPVHLRALLQSDSD
LPAADLVLSATAPMSRAMARQAQAGLAAPLLEIYGSTETGAMATRKTADTDEWTLLPGITLESRSDHAGDGESEPAIRVR
GGHIDEPVALGDAIAPLDAGRFVLHGRKADMIKIAGKRTSLAYLNQQLTAIDGVQDGTFYVPDDVHEVEAAGRVVRLVAL
AVAPGVPAARILQELRERIDAAFLPRPLCLVDRLPRNAAGKLPREALAPLVAAQLAQRHAPGFVIDAGHPAIAGHFPGNP
VVPGVVLLDHAILQIGAALGQPLAVAQVGTVKFLSPVRPGERVEVAHQLHGDHAGTASIRFTLRAGGRDVASGTLSTRAT
LASREASPC

Specific function: Converts p-coumaric acid into p-coumaryl CoA. This is necessary for the activation of the photoactive yellow protein (PYP) chromophore [H]

COG id: COG0365

COG function: function code I; Acyl-coenzyme A synthetases/AMP-(fatty) acid ligases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012743
- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.12 [H]

Molecular weight: Translated: 59891; Mature: 59760

Theoretical pI: Translated: 6.99; Mature: 6.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSTFALLSHPDPDSVVAWRGSQPVTASRFLADVHALAARLPPGDHVFNACADRYRFAVG
CCCCEEEEECCCCCCEEEECCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
LCASLLCGKVSLLPSAQTPETVRQLAAFAPDVFCLYDDADGSVALPRFFYDASEPEPSAP
HHHHHHHCCHHCCCCCCCHHHHHHHHHHCCCEEEEEECCCCCEECHHHCCCCCCCCCCCC
LPVPDIPAERLMAYVFTSGTTGQPVAHAKTWGAMVRGARATAQRLGLADGRAWTLVGTLP
CCCCCCCHHHHHHHHEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCC
PQHMFGLEATVMLALQGSAALASVRAFYPADIRAALRDVPQPRALVTSPVHLRALLQSDS
CHHHHCCCEEEEEEECCCHHHHHHHHHCCHHHHHHHHCCCCCHHHHCCCHHHHHHHHCCC
DLPAADLVLSATAPMSRAMARQAQAGLAAPLLEIYGSTETGAMATRKTADTDEWTLLPGI
CCCHHHEEEECCCHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCEECCCCCCCCEEEECCE
TLESRSDHAGDGESEPAIRVRGGHIDEPVALGDAIAPLDAGRFVLHGRKADMIKIAGKRT
EECCCCCCCCCCCCCCEEEEECCCCCCCHHCCCCCCCCCCCCEEEECCCCCEEEECCCCH
SLAYLNQQLTAIDGVQDGTFYVPDDVHEVEAAGRVVRLVALAVAPGVPAARILQELRERI
HHHHHHHHHHHHCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
DAAFLPRPLCLVDRLPRNAAGKLPREALAPLVAAQLAQRHAPGFVIDAGHPAIAGHFPGN
HHHHCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCEEECCCCCC
PVVPGVVLLDHAILQIGAALGQPLAVAQVGTVKFLSPVRPGERVEVAHQLHGDHAGTASI
CCCCHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHCCCCCCHHHHHHHHHCCCCCCCEEE
RFTLRAGGRDVASGTLSTRATLASREASPC
EEEEECCCCCCCCCCCHHHHHHHCCCCCCC
>Mature Secondary Structure 
TSTFALLSHPDPDSVVAWRGSQPVTASRFLADVHALAARLPPGDHVFNACADRYRFAVG
CCCEEEEECCCCCCEEEECCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
LCASLLCGKVSLLPSAQTPETVRQLAAFAPDVFCLYDDADGSVALPRFFYDASEPEPSAP
HHHHHHHCCHHCCCCCCCHHHHHHHHHHCCCEEEEEECCCCCEECHHHCCCCCCCCCCCC
LPVPDIPAERLMAYVFTSGTTGQPVAHAKTWGAMVRGARATAQRLGLADGRAWTLVGTLP
CCCCCCCHHHHHHHHEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCC
PQHMFGLEATVMLALQGSAALASVRAFYPADIRAALRDVPQPRALVTSPVHLRALLQSDS
CHHHHCCCEEEEEEECCCHHHHHHHHHCCHHHHHHHHCCCCCHHHHCCCHHHHHHHHCCC
DLPAADLVLSATAPMSRAMARQAQAGLAAPLLEIYGSTETGAMATRKTADTDEWTLLPGI
CCCHHHEEEECCCHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCEECCCCCCCCEEEECCE
TLESRSDHAGDGESEPAIRVRGGHIDEPVALGDAIAPLDAGRFVLHGRKADMIKIAGKRT
EECCCCCCCCCCCCCCEEEEECCCCCCCHHCCCCCCCCCCCCEEEECCCCCEEEECCCCH
SLAYLNQQLTAIDGVQDGTFYVPDDVHEVEAAGRVVRLVALAVAPGVPAARILQELRERI
HHHHHHHHHHHHCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
DAAFLPRPLCLVDRLPRNAAGKLPREALAPLVAAQLAQRHAPGFVIDAGHPAIAGHFPGN
HHHHCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCEEECCCCCC
PVVPGVVLLDHAILQIGAALGQPLAVAQVGTVKFLSPVRPGERVEVAHQLHGDHAGTASI
CCCCHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHCCCCCCHHHHHHHHHCCCCCCCEEE
RFTLRAGGRDVASGTLSTRATLASREASPC
EEEEECCCCCCCCCCCHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA