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The map label for this gene is rffG [C]

Identifier: 73537691

GI number: 73537691

Start: 472386

End: 473363

Strand: Reverse

Name: rffG [C]

Synonym: Reut_B3856

Alternate gene names: 73537691

Gene position: 473363-472386 (Counterclockwise)

Preceding gene: 73537693

Following gene: 73537690

Centisome position: 17.36

GC content: 63.29

Gene sequence:

>978_bases
ATGGCGGACTCTGAACGATTTCTGGTAACCGGGGCATTGGGGTGTATCGGCGCATGGACGGTTCGCCGGCTCGCAAACGA
GGGCGTGCCAGTCGTTGCCTTCGACGCAGACAATGACGTGCGACGCCTGCGCCTGCTGATGGACGACGCACAACGGTCGC
GCGTGGAAATGGTTCGGGGCGACATCACGGACCTGAGCTTGCTGGAGGCAACGCTCGATCACCATCGCATTACGCATGTC
ATTCACCTGGCCGCACTGATCCATCCAAGGTTCAAGAGCGATCCACCGCTTGGCGCCCGCGTCAACGTGCTCGGCGGCGT
GAATCTGTTCGAGGCCGTAGCGCGGCGCGTGGAGCGAATTCCTCGCATCGTGTATGCGTCATCGATTGCGGTGTACGACA
ACGGCGACGCGGCCAACGATGCAGCCATCCAGCACCACACAGCCGGGCATCCGACAACCCTATACGGTGTGTTCAAGCAA
GCCGAAGAGGGCATGGCGCGCGTGTATTACCAGGACAGAGGGGTGTCCAGCATCGGCCTTCGCCCGGCGACGGTTTTTGG
CGCCGGAAGAGACTATGGACTGACTGCGGCCCCCACGCAGGCAGTCCTGGCGGCAGCGCTCGGACGCCCTTTCCATATTC
CGTACAGCGGCCGCAGCCAGATCCACTATGCCGACGACCTTGCGCGCATGTTCATCGCTTGCGCACGTTCGGGCTATCGC
GGTGCGGACGTCTTCAATATGCATGGCAGCGTCGCGGACATGGGCGAGATCGTCGAGACGATCGAGTCGGTCATTCCGCA
GGCGCGCGGAACGATCACGTTCGACGGGGCATCGCTCGGGCTTCCGGAAGACTACGACGCGACCGCGCTCGAGAACGTGA
TTGGTCCGCTGCCCCGCACACCGCTGCGCGAAGCCGTGGGCGAGACCTTCAGGCTCTTTCGCAGCAGGATCGCCAGCGGC
GAAATCCGCGACACCTGA

Upstream 100 bases:

>100_bases
CCACGCCCCACCACGTCAACCTCAATCCAAATCCCCTTACTACACTGCTGACAGTTCAATCCGGGCACACTGCGTTGCCC
GCAAACGGTGACTGGCAGCT

Downstream 100 bases:

>100_bases
TCCGTTGATGGCGGCAGTACTGCCGCGCCGCGGATCTGCGCATCTCCCGACGCATCCCCTCGTTTTCCCGAACTGTCCCG
AAGGGCATCAAGGTTGTCCC

Product: NAD-dependent epimerase/dehydratase

Products: dTDP-4-dehydro-6-deoxy-D-glucose; H2O [C]

Alternate protein names: Nucleotide Sugar Epimerase; UDP-Glucose 4-Epimerase; Capsular Polysaccharide Biosynthesis Protein I; Nucleoside-Diphosphate-Sugar Epimerase; NAD-Dependent Epimerase/Dehydratase Family Protein; NAD Dependent Epimerase/Dehydratase Family Protein; UDP-Glucuronate 5-Epimerase; NAD Dependent Epimerase/Dehydratase Family; Epimerase; UDP-Glucuronic Acid Epimerase; Capsular Polysaccharide Biosynthesis Protein; Nucleoside-Diphosphate-Sugar Epimerases; UDP-Sugar Epimerase; NAD Dependent Epimerase/Dehydratase; Nucleoside-Diphosphate-Sugar Epimerases Protein; Dehydrogenase Family Protein; Oligopeptide Transporter OPT; UDP-GlcNAc C4 Epimerase; Uridine Diphosphate Galacturonate 4-Epimerase; Capsular Polysaccharide Biosynthesis Protein Cap I; Nucleotide Sugar Epimerase/Dehydratase; NAD_dependent Epimerase/Dehydratase; Nucleotide-Diphosphate Sugar Epimerase; UDP-Glucuronat Epimerase; UDP-Glucuronate 4-Epimerase; UDP-N-Acetylglucosamine 4-Epimerase; Udp-Glucuronic Acid Epimerase; NDP-Sugar Epimerase; Polysaccharide Biosynthesis Protein; VipB-Like Polysaccharide Biosynthesis Protein; Nucleotide-Sugar Epimerase; UDP-Galacturonate 4-Epimerase

Number of amino acids: Translated: 325; Mature: 324

Protein sequence:

>325_residues
MADSERFLVTGALGCIGAWTVRRLANEGVPVVAFDADNDVRRLRLLMDDAQRSRVEMVRGDITDLSLLEATLDHHRITHV
IHLAALIHPRFKSDPPLGARVNVLGGVNLFEAVARRVERIPRIVYASSIAVYDNGDAANDAAIQHHTAGHPTTLYGVFKQ
AEEGMARVYYQDRGVSSIGLRPATVFGAGRDYGLTAAPTQAVLAAALGRPFHIPYSGRSQIHYADDLARMFIACARSGYR
GADVFNMHGSVADMGEIVETIESVIPQARGTITFDGASLGLPEDYDATALENVIGPLPRTPLREAVGETFRLFRSRIASG
EIRDT

Sequences:

>Translated_325_residues
MADSERFLVTGALGCIGAWTVRRLANEGVPVVAFDADNDVRRLRLLMDDAQRSRVEMVRGDITDLSLLEATLDHHRITHV
IHLAALIHPRFKSDPPLGARVNVLGGVNLFEAVARRVERIPRIVYASSIAVYDNGDAANDAAIQHHTAGHPTTLYGVFKQ
AEEGMARVYYQDRGVSSIGLRPATVFGAGRDYGLTAAPTQAVLAAALGRPFHIPYSGRSQIHYADDLARMFIACARSGYR
GADVFNMHGSVADMGEIVETIESVIPQARGTITFDGASLGLPEDYDATALENVIGPLPRTPLREAVGETFRLFRSRIASG
EIRDT
>Mature_324_residues
ADSERFLVTGALGCIGAWTVRRLANEGVPVVAFDADNDVRRLRLLMDDAQRSRVEMVRGDITDLSLLEATLDHHRITHVI
HLAALIHPRFKSDPPLGARVNVLGGVNLFEAVARRVERIPRIVYASSIAVYDNGDAANDAAIQHHTAGHPTTLYGVFKQA
EEGMARVYYQDRGVSSIGLRPATVFGAGRDYGLTAAPTQAVLAAALGRPFHIPYSGRSQIHYADDLARMFIACARSGYRG
ADVFNMHGSVADMGEIVETIESVIPQARGTITFDGASLGLPEDYDATALENVIGPLPRTPLREAVGETFRLFRSRIASGE
IRDT

Specific function: INVOLVED IN THE SYNTHESIS OF ENTEROBACTERIAL COMMON ANTIGEN (ECA) AND REQUIRED FOR SYNTHESIS OF LIPOPOLYSACCHARIDE O-SIDE CHAINS. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 4.2.1.46 [C]

Molecular weight: Translated: 35317; Mature: 35186

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADSERFLVTGALGCIGAWTVRRLANEGVPVVAFDADNDVRRLRLLMDDAQRSRVEMVRG
CCCCCCEEEEHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHC
DITDLSLLEATLDHHRITHVIHLAALIHPRFKSDPPLGARVNVLGGVNLFEAVARRVERI
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHC
PRIVYASSIAVYDNGDAANDAAIQHHTAGHPTTLYGVFKQAEEGMARVYYQDRGVSSIGL
CHHEEECEEEEEECCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHEEHHHCCCCCCCCC
RPATVFGAGRDYGLTAAPTQAVLAAALGRPFHIPYSGRSQIHYADDLARMFIACARSGYR
CCCEEEECCCCCCCCCCHHHHHHHHHHCCCEECCCCCCCCEEHHHHHHHHHHHHHHCCCC
GADVFNMHGSVADMGEIVETIESVIPQARGTITFDGASLGLPEDYDATALENVIGPLPRT
CCCEEECCCCHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCHHHHHHHHCCCCCC
PLREAVGETFRLFRSRIASGEIRDT
HHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
ADSERFLVTGALGCIGAWTVRRLANEGVPVVAFDADNDVRRLRLLMDDAQRSRVEMVRG
CCCCCEEEEHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHC
DITDLSLLEATLDHHRITHVIHLAALIHPRFKSDPPLGARVNVLGGVNLFEAVARRVERI
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHC
PRIVYASSIAVYDNGDAANDAAIQHHTAGHPTTLYGVFKQAEEGMARVYYQDRGVSSIGL
CHHEEECEEEEEECCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHEEHHHCCCCCCCCC
RPATVFGAGRDYGLTAAPTQAVLAAALGRPFHIPYSGRSQIHYADDLARMFIACARSGYR
CCCEEEECCCCCCCCCCHHHHHHHHHHCCCEECCCCCCCCEEHHHHHHHHHHHHHHCCCC
GADVFNMHGSVADMGEIVETIESVIPQARGTITFDGASLGLPEDYDATALENVIGPLPRT
CCCEEECCCCHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCHHHHHHHHCCCCCC
PLREAVGETFRLFRSRIASGEIRDT
HHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.093 {dTDPglucose}} [C]

Substrates: dTDPglucose [C]

Specific reaction: dTDPglucose --> dTDP-4-dehydro-6-deoxy-D-glucose + H2O [C]

General reaction: Elimination (of H2O C-O bond cleavage [C]

Inhibitor: p-Chloromercuribenzoate; TMP [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA