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The map label for this gene is gudD [H]

Identifier: 73537550

GI number: 73537550

Start: 303685

End: 305055

Strand: Direct

Name: gudD [H]

Synonym: Reut_B3715

Alternate gene names: 73537550

Gene position: 303685-305055 (Clockwise)

Preceding gene: 73537547

Following gene: 73537551

Centisome position: 11.14

GC content: 67.4

Gene sequence:

>1371_bases
ATGAACGCCACGCAAGCCGCCATCCCTGGCATTCCCGGCAACAGCGCCGCCACGCCGGTCGTGACCGAACTGACCGTGGT
GCCGGTCGCCGGCCACGACAGCATGCTGATGAACCTGTCGGGCGCGCATGGCCCGTACTTCACGCGCAATATCGTGATCC
TGCGCGACAGCGCGGGCCATACCGGCGTGGGCGAAGTGCCGGGCGGGGAGGGTATCCGACAGACGCTCGAAGACGCGCGG
ACGCTGGTGGTGGGGCAACCCATCGGCCAGTACCAGGCCGTGCTCAACCGCGCGCGTGCCGCGTTTGCCGGGCGCGACGC
GGGCGGCCGCGGCCTGCAGACGTTCGATCTGCGCATTGCCATCCACGCCGTGACGGCGCTGGAAGCGGCGCTGCTCGATC
TGCTTGGCAAGCATTTGCAGGTTCCCGTGGCGGCGCTGCTTGGCGAAGGCCAGCAGCGTGATGCGGTCGAGATGCTCGGC
TACCTGTTCTATATCGGCGACCGCCGCCGCACCACGCTCGACTACCGTACCGAGCCGGATGCCGGCAACGACTGGTTCCG
CCTGCGCAATGAAGAAGCCATGACGCCCGAGGCCGTCGTGCGCCTGGCCGAGGCCGCTTACGAGCGCTATGGCTTCAATG
ACTTCAAGCTCAAGGGCGGCGTGCTGCGCGGCGACGAGGAGATGGAAGCGATCATCGCGCTGCACGAGCGCTTCCCGAAA
GCGCGCGTCACGCTCGATCCGAACGGCGGCTGGCTGCTGGCCGACGCGATCCGCCTGTGCCGCGACAAGCACGGTGTGCT
GGCCTACGCGGAAGATCCGTGCGGCGCCGAAGACGGCTACTCTGGCCGCGAGATCATGGCCGAGTTCCGCGCGGCGACGG
GCTTGCCGACCGCCACCAACATGATCGCCACCGACTGGCGCCAGATGGGCCATGCGGTGCGGCTGCAATCGGTCGATATC
CCGCTGGCCGATCCGCATTTCTGGACCATGCAGGGCTCGGTGCGCGTTGCGCAGATGTGCGCCGAGTGGGGCCTGACATG
GGGCTCGCATTCCAACAACCACTTCGATATCTCGCTGGCGATGTTCACTCACGTGGCCGCCGCCGCGCCGGGCCGTATTA
CCGCGATCGACACGCACTGGATCTGGCAGGACGGCGAACACCTGACGCGCGACCCGCTGAAGATCGAAGGCGGCATGGTG
CAGGTGCCCAAGACGCCGGGCCTTGGCGTGGAGCTGGACATGGACGCGCTCGCGCGCGCCAACGCGCTCTACCAGGAGAA
GGGCCTCGGCGCCCGCGATGATGCGATCGCCATGCAGTTCCTGATTCCGGGCTGGAATTTCAACAACAAGGCACCCTGCA
TGGTCCGCTAG

Upstream 100 bases:

>100_bases
TATACTCGGTTGTAAGTCATACGACGACTGATAACGTATGCTAAAGCATTCAGCATGCGCCGCCAACAGCGCATGCCAAT
CAGACCCTGGAGCAGCCCGC

Downstream 100 bases:

>100_bases
GGCTGCTGCACAGCCACCACGCAACACACAGCGACATAAAACAACAGGAGACAACCATGATCAAACTTTCCACGCTGCTG
CGCCGCTGTGCGGGCGCGGG

Product: D-glucarate dehydratase

Products: NA

Alternate protein names: GDH; GlucD [H]

Number of amino acids: Translated: 456; Mature: 456

Protein sequence:

>456_residues
MNATQAAIPGIPGNSAATPVVTELTVVPVAGHDSMLMNLSGAHGPYFTRNIVILRDSAGHTGVGEVPGGEGIRQTLEDAR
TLVVGQPIGQYQAVLNRARAAFAGRDAGGRGLQTFDLRIAIHAVTALEAALLDLLGKHLQVPVAALLGEGQQRDAVEMLG
YLFYIGDRRRTTLDYRTEPDAGNDWFRLRNEEAMTPEAVVRLAEAAYERYGFNDFKLKGGVLRGDEEMEAIIALHERFPK
ARVTLDPNGGWLLADAIRLCRDKHGVLAYAEDPCGAEDGYSGREIMAEFRAATGLPTATNMIATDWRQMGHAVRLQSVDI
PLADPHFWTMQGSVRVAQMCAEWGLTWGSHSNNHFDISLAMFTHVAAAAPGRITAIDTHWIWQDGEHLTRDPLKIEGGMV
QVPKTPGLGVELDMDALARANALYQEKGLGARDDAIAMQFLIPGWNFNNKAPCMVR

Sequences:

>Translated_456_residues
MNATQAAIPGIPGNSAATPVVTELTVVPVAGHDSMLMNLSGAHGPYFTRNIVILRDSAGHTGVGEVPGGEGIRQTLEDAR
TLVVGQPIGQYQAVLNRARAAFAGRDAGGRGLQTFDLRIAIHAVTALEAALLDLLGKHLQVPVAALLGEGQQRDAVEMLG
YLFYIGDRRRTTLDYRTEPDAGNDWFRLRNEEAMTPEAVVRLAEAAYERYGFNDFKLKGGVLRGDEEMEAIIALHERFPK
ARVTLDPNGGWLLADAIRLCRDKHGVLAYAEDPCGAEDGYSGREIMAEFRAATGLPTATNMIATDWRQMGHAVRLQSVDI
PLADPHFWTMQGSVRVAQMCAEWGLTWGSHSNNHFDISLAMFTHVAAAAPGRITAIDTHWIWQDGEHLTRDPLKIEGGMV
QVPKTPGLGVELDMDALARANALYQEKGLGARDDAIAMQFLIPGWNFNNKAPCMVR
>Mature_456_residues
MNATQAAIPGIPGNSAATPVVTELTVVPVAGHDSMLMNLSGAHGPYFTRNIVILRDSAGHTGVGEVPGGEGIRQTLEDAR
TLVVGQPIGQYQAVLNRARAAFAGRDAGGRGLQTFDLRIAIHAVTALEAALLDLLGKHLQVPVAALLGEGQQRDAVEMLG
YLFYIGDRRRTTLDYRTEPDAGNDWFRLRNEEAMTPEAVVRLAEAAYERYGFNDFKLKGGVLRGDEEMEAIIALHERFPK
ARVTLDPNGGWLLADAIRLCRDKHGVLAYAEDPCGAEDGYSGREIMAEFRAATGLPTATNMIATDWRQMGHAVRLQSVDI
PLADPHFWTMQGSVRVAQMCAEWGLTWGSHSNNHFDISLAMFTHVAAAAPGRITAIDTHWIWQDGEHLTRDPLKIEGGMV
QVPKTPGLGVELDMDALARANALYQEKGLGARDDAIAMQFLIPGWNFNNKAPCMVR

Specific function: Catalyzes the dehydration of glucarate to 5-keto-4- deoxy-D-glucarate (5-kdGluc) [H]

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family. GlucD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789150, Length=440, Percent_Identity=74.0909090909091, Blast_Score=702, Evalue=0.0,
Organism=Escherichia coli, GI1789151, Length=445, Percent_Identity=61.123595505618, Blast_Score=540, Evalue=1e-155,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017653
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: =4.2.1.40 [H]

Molecular weight: Translated: 49637; Mature: 49637

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNATQAAIPGIPGNSAATPVVTELTVVPVAGHDSMLMNLSGAHGPYFTRNIVILRDSAGH
CCCCCCCCCCCCCCCCCCCEEEEEEEEEECCCCCEEEEECCCCCCEEEEEEEEEECCCCC
TGVGEVPGGEGIRQTLEDARTLVVGQPIGQYQAVLNRARAAFAGRDAGGRGLQTFDLRIA
CCCCCCCCCHHHHHHHHHHHEEEECCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEE
IHAVTALEAALLDLLGKHLQVPVAALLGEGQQRDAVEMLGYLFYIGDRRRTTLDYRTEPD
EHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCHHHHHHHHHEEECCCCEEEEEECCCCC
AGNDWFRLRNEEAMTPEAVVRLAEAAYERYGFNDFKLKGGVLRGDEEMEAIIALHERFPK
CCCCCEEECCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCEECCCHHHHHHHHHHHHCCC
ARVTLDPNGGWLLADAIRLCRDKHGVLAYAEDPCGAEDGYSGREIMAEFRAATGLPTATN
EEEEECCCCCEEHHHHHHHHHCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCHHH
MIATDWRQMGHAVRLQSVDIPLADPHFWTMQGSVRVAQMCAEWGLTWGSHSNNHFDISLA
HHHHHHHHCCCEEEEEECCCCCCCCCEEEECCCHHHHHHHHHHCCCCCCCCCCEEEEEHH
MFTHVAAAAPGRITAIDTHWIWQDGEHLTRDPLKIEGGMVQVPKTPGLGVELDMDALARA
HHHHHHHCCCCCEEEEEEEEEECCCCCCCCCCEEECCCEEEECCCCCCCEEECHHHHHHH
NALYQEKGLGARDDAIAMQFLIPGWNFNNKAPCMVR
HHHHHHCCCCCCCCCEEEEEEECCCCCCCCCCCEEC
>Mature Secondary Structure
MNATQAAIPGIPGNSAATPVVTELTVVPVAGHDSMLMNLSGAHGPYFTRNIVILRDSAGH
CCCCCCCCCCCCCCCCCCCEEEEEEEEEECCCCCEEEEECCCCCCEEEEEEEEEECCCCC
TGVGEVPGGEGIRQTLEDARTLVVGQPIGQYQAVLNRARAAFAGRDAGGRGLQTFDLRIA
CCCCCCCCCHHHHHHHHHHHEEEECCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEE
IHAVTALEAALLDLLGKHLQVPVAALLGEGQQRDAVEMLGYLFYIGDRRRTTLDYRTEPD
EHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCHHHHHHHHHEEECCCCEEEEEECCCCC
AGNDWFRLRNEEAMTPEAVVRLAEAAYERYGFNDFKLKGGVLRGDEEMEAIIALHERFPK
CCCCCEEECCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCEECCCHHHHHHHHHHHHCCC
ARVTLDPNGGWLLADAIRLCRDKHGVLAYAEDPCGAEDGYSGREIMAEFRAATGLPTATN
EEEEECCCCCEEHHHHHHHHHCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCHHH
MIATDWRQMGHAVRLQSVDIPLADPHFWTMQGSVRVAQMCAEWGLTWGSHSNNHFDISLA
HHHHHHHHCCCEEEEEECCCCCCCCCEEEECCCHHHHHHHHHHCCCCCCCCCCEEEEEHH
MFTHVAAAAPGRITAIDTHWIWQDGEHLTRDPLKIEGGMVQVPKTPGLGVELDMDALARA
HHHHHHHCCCCCEEEEEEEEEECCCCCCCCCCEEECCCEEEECCCCCCCEEECHHHHHHH
NALYQEKGLGARDDAIAMQFLIPGWNFNNKAPCMVR
HHHHHHCCCCCCCCCEEEEEEECCCCCCCCCCCEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9772161 [H]