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The map label for this gene is acnB [H]

Identifier: 73537299

GI number: 73537299

Start: 127

End: 2562

Strand: Direct

Name: acnB [H]

Synonym: Reut_B3464

Alternate gene names: 73537299

Gene position: 127-2562 (Clockwise)

Preceding gene: NA

Following gene: 73537300

Centisome position: 0.0

GC content: 63.88

Gene sequence:

>2436_bases
GTGCCTGCCGGTGTGGATGACGCCGCCAAGGTCAAGGCTGCCTTCCTGGCCGCCGTTGCCCAGGGCAAGGACAGCTGCAA
GCTGATCTCGCGCGCCAAGGCGACCGAACTGCTGGGCACCATGCTCGGCGGCTACAACATCGCCCCGCTGATTTCGCTGC
TGGCCGATGCCGAAGTCGGCACCGTCGCCGCTGATGCGCTCAAGAAGACCCTGCTGATGTTCGACGCCTTCCACGACGTC
AAGGAACTCGCAGACAAGGGCAACGCCAACGCCAAGGCCGTGCTGCAGAGCTGGGCCGACGCCGAGTGGTTCACGAGCCG
CCCGGAAGTGCCGCAGAGCCTGACCATCACCGTGTTCAAGGTGCCGGGCGAAACCAACACCGACGACCTGTCGCCGGCTC
CGGACGCCACCACGCGCCCGGACATCCCGATGCACGCGCTGGCCATGCTGAAGAACAAGCGTGAAGGCGCGGCGTTCCAG
CCCGAAGAAGACGGCAAGCGCGGCCCGATCAAGTTCATCCAGTCGCTGGCCGAGAAGGGCAATCTTGTTGCCTACGTCGG
CGACGTGGTCGGTACCGGTTCGAGCCGCAAGTCGGCCACTAACTCGGTGCTGTGGTTCACCGGCGAAGACATCCCCTTCA
TCCCGAACAAGCGCTTTGGCGGCGTGTGCCTGGGCAGCAAGATCGCCCCGATCTTCTACAACACTATGGAAGACGCCGGC
GCGCTGCCGATCGAGCTGGACGTGTCGAAGATGGAAATGGGCGACGTGGTGGAACTGCGCCCGTACGAAGGCAAGGCCCT
GAAGAACGGTGAAGTGATCGCCGAGTTCAAGGTCAAGTCCGACGTGCTGTTCGACGAAGTCCGCGCCGGCGGCCGCATTC
CGCTGATCGTCGGCCGTGGCCTGACCGCCAAGGCCCGCGAAGCGTTGGGCCTGGCCCCGTCGACGCTGTTCCGCCTGCCG
CAGAACCCGGCCGACACTGGCAAGGGCTTCACGCTGGCCCAGAAGATGGTCGGCCGCGCCTGCGGCCTGCCGGAAGGCAA
GGGCATCCGTCCGGGCACGTACTGCGAACCGAAGATGACCTCGGTGGGCTCGCAAGACACCACCGGCCCGATGACCCGCG
ACGAGCTGAAGGACCTGGCCTGCCTGGGCTTCTCGGCCGACCTGGTGATGCAGTCGTTCTGCCACACTGCGGCGTATCCG
AAGCCGGTCGACGTCAAGACCCACCACACGCTGCCCGAATTCATTTCGACCCGCGGCGGTATCTCGCTGCGTCCGGGCGA
CGGCGTGATCCACTCGTGGCTGAACCGCATGCTGCTGCCCGACACCGTCGGCACGGGCGGCGACTCGCACACCCGCTTCC
CGATCGGCATCAGCTTCCCGGCCGGTTCGGGCCTGGTCGCCTTTGCCGCTGCCACCGGCGTGATGCCGCTGGACATGCCG
GAATCGGTGCTGGTCCGCTTCAAGGGCAAGATGCAGCCTGGCGTGACCCTGCGTGACCTGGTCAACGCGATCCCGCTGTA
CGCAATCAAGCAAGGCCTGCTGACCGTGGCCAAGCAAGGCAAGACGAACATCTTCTCGGGCCGCATCCTCGAAATCGAAG
GCCTGCCTGACCTGAAGGTCGAGCAAGCGTTCGAACTGTCAGATGCTTCCGCCGAGCGTTCGGCCGCCGGTTGCTCGGTG
CACCTGAACAAGGAACCGATCATCGAATACATCAACAGCAACATCACGCTGCTGAAGTGGATGATCGCCCAGGGCTACCA
GGACCCGCGCAGCCTGCAGCGCCGCATCGAAGCCATGCAAGCATGGCTGGCCAAGCCGGAACTGCTGGCTCCGGATGCCG
ACGCCGAATACGCTGCCGTGATCGAGATCGACCTGGCCGACGTGCATGAGCCGATCGTTGCCTGCCCGAACGACCCGGAT
GACGTGAAGACGCTGTCGGAAGTTGCCGGCGCCAAGATCGACGAAGTGTTCATCGGTTCGTGCATGACCAACATCGGCCA
CTTCCGTGCCGCTTCGAAGCTGCTGGAAGGCAAGCGCGACATCCCGGTCAAGCTGTGGGTGGCCCCGCCGACCAAGATGG
ACCAGAAGCAGCTGACCGAAGAAGGCCACTACGGCGTGTTCGGCACGGCTGGTGCACGTACCGAAATGCCGGGCTGCTCG
CTGTGCATGGGTAACCAGGCACAAGTGCGCGAAGGCGCCACGGTGATGTCGACCTCGACCCGCAACTTCCCGAACCGTCT
GGGCAAGAACACCAACGTGTACCTGGGTTCGGCCGAACTGGCCGCGATCTGCTCGCGTCTGGGCAAGATCCCTTCGAAGG
AAGAGTACATGGCCGATATTGGCGTCATTAACCAGAATGGCGACAAGATCTACAAGTACATGAACTTCGACCAGATCGAG
GACTTCAAGGAAGTCGCTGATGGCGTGAACCTGTAA

Upstream 100 bases:

>100_bases
GCTGCCGCTGTCGGCAAAGCAGACCGCCGAGCTGGTCGACCTGCTGAAGAACCCGCCGGCCGGCGAAGAACAGGTCCTGG
TCGACCTGATCACCAACCGC

Downstream 100 bases:

>100_bases
GGTTCTCCGCGGCACCCCGACGCCACCACCACCAATGACCGCCCCCTGTTTTCGGGGGCGCCATTGGCGACAACGGATGC
ATAGTCGGGTGTGAGAATTT

Product: aconitate hydratase

Products: NA

Alternate protein names: Aconitase 2; 2-methylisocitrate dehydratase; Citrate hydro-lyase 2 [H]

Number of amino acids: Translated: 811; Mature: 810

Protein sequence:

>811_residues
MPAGVDDAAKVKAAFLAAVAQGKDSCKLISRAKATELLGTMLGGYNIAPLISLLADAEVGTVAADALKKTLLMFDAFHDV
KELADKGNANAKAVLQSWADAEWFTSRPEVPQSLTITVFKVPGETNTDDLSPAPDATTRPDIPMHALAMLKNKREGAAFQ
PEEDGKRGPIKFIQSLAEKGNLVAYVGDVVGTGSSRKSATNSVLWFTGEDIPFIPNKRFGGVCLGSKIAPIFYNTMEDAG
ALPIELDVSKMEMGDVVELRPYEGKALKNGEVIAEFKVKSDVLFDEVRAGGRIPLIVGRGLTAKAREALGLAPSTLFRLP
QNPADTGKGFTLAQKMVGRACGLPEGKGIRPGTYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYP
KPVDVKTHHTLPEFISTRGGISLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISFPAGSGLVAFAAATGVMPLDMP
ESVLVRFKGKMQPGVTLRDLVNAIPLYAIKQGLLTVAKQGKTNIFSGRILEIEGLPDLKVEQAFELSDASAERSAAGCSV
HLNKEPIIEYINSNITLLKWMIAQGYQDPRSLQRRIEAMQAWLAKPELLAPDADAEYAAVIEIDLADVHEPIVACPNDPD
DVKTLSEVAGAKIDEVFIGSCMTNIGHFRAASKLLEGKRDIPVKLWVAPPTKMDQKQLTEEGHYGVFGTAGARTEMPGCS
LCMGNQAQVREGATVMSTSTRNFPNRLGKNTNVYLGSAELAAICSRLGKIPSKEEYMADIGVINQNGDKIYKYMNFDQIE
DFKEVADGVNL

Sequences:

>Translated_811_residues
MPAGVDDAAKVKAAFLAAVAQGKDSCKLISRAKATELLGTMLGGYNIAPLISLLADAEVGTVAADALKKTLLMFDAFHDV
KELADKGNANAKAVLQSWADAEWFTSRPEVPQSLTITVFKVPGETNTDDLSPAPDATTRPDIPMHALAMLKNKREGAAFQ
PEEDGKRGPIKFIQSLAEKGNLVAYVGDVVGTGSSRKSATNSVLWFTGEDIPFIPNKRFGGVCLGSKIAPIFYNTMEDAG
ALPIELDVSKMEMGDVVELRPYEGKALKNGEVIAEFKVKSDVLFDEVRAGGRIPLIVGRGLTAKAREALGLAPSTLFRLP
QNPADTGKGFTLAQKMVGRACGLPEGKGIRPGTYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYP
KPVDVKTHHTLPEFISTRGGISLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISFPAGSGLVAFAAATGVMPLDMP
ESVLVRFKGKMQPGVTLRDLVNAIPLYAIKQGLLTVAKQGKTNIFSGRILEIEGLPDLKVEQAFELSDASAERSAAGCSV
HLNKEPIIEYINSNITLLKWMIAQGYQDPRSLQRRIEAMQAWLAKPELLAPDADAEYAAVIEIDLADVHEPIVACPNDPD
DVKTLSEVAGAKIDEVFIGSCMTNIGHFRAASKLLEGKRDIPVKLWVAPPTKMDQKQLTEEGHYGVFGTAGARTEMPGCS
LCMGNQAQVREGATVMSTSTRNFPNRLGKNTNVYLGSAELAAICSRLGKIPSKEEYMADIGVINQNGDKIYKYMNFDQIE
DFKEVADGVNL
>Mature_810_residues
PAGVDDAAKVKAAFLAAVAQGKDSCKLISRAKATELLGTMLGGYNIAPLISLLADAEVGTVAADALKKTLLMFDAFHDVK
ELADKGNANAKAVLQSWADAEWFTSRPEVPQSLTITVFKVPGETNTDDLSPAPDATTRPDIPMHALAMLKNKREGAAFQP
EEDGKRGPIKFIQSLAEKGNLVAYVGDVVGTGSSRKSATNSVLWFTGEDIPFIPNKRFGGVCLGSKIAPIFYNTMEDAGA
LPIELDVSKMEMGDVVELRPYEGKALKNGEVIAEFKVKSDVLFDEVRAGGRIPLIVGRGLTAKAREALGLAPSTLFRLPQ
NPADTGKGFTLAQKMVGRACGLPEGKGIRPGTYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
PVDVKTHHTLPEFISTRGGISLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISFPAGSGLVAFAAATGVMPLDMPE
SVLVRFKGKMQPGVTLRDLVNAIPLYAIKQGLLTVAKQGKTNIFSGRILEIEGLPDLKVEQAFELSDASAERSAAGCSVH
LNKEPIIEYINSNITLLKWMIAQGYQDPRSLQRRIEAMQAWLAKPELLAPDADAEYAAVIEIDLADVHEPIVACPNDPDD
VKTLSEVAGAKIDEVFIGSCMTNIGHFRAASKLLEGKRDIPVKLWVAPPTKMDQKQLTEEGHYGVFGTAGARTEMPGCSL
CMGNQAQVREGATVMSTSTRNFPNRLGKNTNVYLGSAELAAICSRLGKIPSKEEYMADIGVINQNGDKIYKYMNFDQIED
FKEVADGVNL

Specific function: Catalyzes the isomerization of citrate to isocitrate via cis-aconitate as well as the dehydration of 2-methylisocitrate to cis-2-methylaconitate [H]

COG id: COG1049

COG function: function code C; Aconitase B

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family [H]

Homologues:

Organism=Escherichia coli, GI2367097, Length=812, Percent_Identity=72.9064039408867, Blast_Score=1191, Evalue=0.0,
Organism=Escherichia coli, GI1786259, Length=366, Percent_Identity=26.775956284153, Blast_Score=75, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6321429, Length=357, Percent_Identity=25.7703081232493, Blast_Score=66, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015928
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR004406
- InterPro:   IPR015930
- InterPro:   IPR015933
- InterPro:   IPR015929 [H]

Pfam domain/function: PF00330 Aconitase; PF06434 Aconitase_2_N; PF11791 Aconitase_B_N [H]

EC number: =4.2.1.3; =4.2.1.99 [H]

Molecular weight: Translated: 87144; Mature: 87013

Theoretical pI: Translated: 5.76; Mature: 5.76

Prosite motif: PS00450 ACONITASE_1 ; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAGVDDAAKVKAAFLAAVAQGKDSCKLISRAKATELLGTMLGGYNIAPLISLLADAEVG
CCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHH
TVAADALKKTLLMFDAFHDVKELADKGNANAKAVLQSWADAEWFTSRPEVPQSLTITVFK
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHCCCCCCCCCCEEEEEEE
VPGETNTDDLSPAPDATTRPDIPMHALAMLKNKREGAAFQPEEDGKRGPIKFIQSLAEKG
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHCC
NLVAYVGDVVGTGSSRKSATNSVLWFTGEDIPFIPNKRFGGVCLGSKIAPIFYNTMEDAG
CEEEEECHHHCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCEEECCCCCHHHHHHHHHCC
ALPIELDVSKMEMGDVVELRPYEGKALKNGEVIAEFKVKSDVLFDEVRAGGRIPLIVGRG
CCEEEEEHHHHCCCCEEEECCCCCCCCCCCCEEEEEEHHHHHHHHHHHCCCCEEEEECCC
LTAKAREALGLAPSTLFRLPQNPADTGKGFTLAQKMVGRACGLPEGKGIRPGTYCEPKMT
CCHHHHHHHCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHH
SVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPKPVDVKTHHTLPEFISTRGG
CCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHCCCC
ISLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISFPAGSGLVAFAAATGVMPLDMP
CEECCCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCCEEEHHHCCCCCCCCC
ESVLVRFKGKMQPGVTLRDLVNAIPLYAIKQGLLTVAKQGKTNIFSGRILEIEGLPDLKV
HHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCEEEEECCCCCCCH
EQAFELSDASAERSAAGCSVHLNKEPIIEYINSNITLLKWMIAQGYQDPRSLQRRIEAMQ
HHHHCCCCCCHHHCCCCCEEEECCHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHH
AWLAKPELLAPDADAEYAAVIEIDLADVHEPIVACPNDPDDVKTLSEVAGAKIDEVFIGS
HHHCCCCCCCCCCCCCEEEEEEEEHHHCCCCEEECCCCHHHHHHHHHHHCCCHHHHHHHH
CMTNIGHFRAASKLLEGKRDIPVKLWVAPPTKMDQKQLTEEGHYGVFGTAGARTEMPGCS
HHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCHHHHHCCCCCCEEECCCCCCCCCCCC
LCMGNQAQVREGATVMSTSTRNFPNRLGKNTNVYLGSAELAAICSRLGKIPSKEEYMADI
EECCCHHHHHCCCEEEECCCCCHHHHHCCCCEEEECCHHHHHHHHHHCCCCCHHHHHHHH
GVINQNGDKIYKYMNFDQIEDFKEVADGVNL
CEEECCCCCEEECCCHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
PAGVDDAAKVKAAFLAAVAQGKDSCKLISRAKATELLGTMLGGYNIAPLISLLADAEVG
CCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHH
TVAADALKKTLLMFDAFHDVKELADKGNANAKAVLQSWADAEWFTSRPEVPQSLTITVFK
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHCCCCCCCCCCEEEEEEE
VPGETNTDDLSPAPDATTRPDIPMHALAMLKNKREGAAFQPEEDGKRGPIKFIQSLAEKG
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHCC
NLVAYVGDVVGTGSSRKSATNSVLWFTGEDIPFIPNKRFGGVCLGSKIAPIFYNTMEDAG
CEEEEECHHHCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCEEECCCCCHHHHHHHHHCC
ALPIELDVSKMEMGDVVELRPYEGKALKNGEVIAEFKVKSDVLFDEVRAGGRIPLIVGRG
CCEEEEEHHHHCCCCEEEECCCCCCCCCCCCEEEEEEHHHHHHHHHHHCCCCEEEEECCC
LTAKAREALGLAPSTLFRLPQNPADTGKGFTLAQKMVGRACGLPEGKGIRPGTYCEPKMT
CCHHHHHHHCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHH
SVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPKPVDVKTHHTLPEFISTRGG
CCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHCCCC
ISLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISFPAGSGLVAFAAATGVMPLDMP
CEECCCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCCEEEHHHCCCCCCCCC
ESVLVRFKGKMQPGVTLRDLVNAIPLYAIKQGLLTVAKQGKTNIFSGRILEIEGLPDLKV
HHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCEEEEECCCCCCCH
EQAFELSDASAERSAAGCSVHLNKEPIIEYINSNITLLKWMIAQGYQDPRSLQRRIEAMQ
HHHHCCCCCCHHHCCCCCEEEECCHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHH
AWLAKPELLAPDADAEYAAVIEIDLADVHEPIVACPNDPDDVKTLSEVAGAKIDEVFIGS
HHHCCCCCCCCCCCCCEEEEEEEEHHHCCCCEEECCCCHHHHHHHHHHHCCCHHHHHHHH
CMTNIGHFRAASKLLEGKRDIPVKLWVAPPTKMDQKQLTEEGHYGVFGTAGARTEMPGCS
HHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCHHHHHCCCCCCEEECCCCCCCCCCCC
LCMGNQAQVREGATVMSTSTRNFPNRLGKNTNVYLGSAELAAICSRLGKIPSKEEYMADI
EECCCHHHHHCCCEEEECCCCCHHHHHCCCCEEEECCHHHHHHHHHHCCCCCHHHHHHHH
GVINQNGDKIYKYMNFDQIEDFKEVADGVNL
CEEECCCCCEEECCCHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]