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The map label for this gene is gatA [H]

Identifier: 71911320

GI number: 71911320

Start: 1461374

End: 1462840

Strand: Reverse

Name: gatA [H]

Synonym: M5005_Spy_1507

Alternate gene names: 71911320

Gene position: 1462840-1461374 (Counterclockwise)

Preceding gene: 71911321

Following gene: 71911319

Centisome position: 79.56

GC content: 43.08

Gene sequence:

>1467_bases
ATGTCTTTTAATCATAAAACCATTGAAGAGTTGCATGACCTCCTTGTAGCTAAGGAGATTTCAGCAACAGAATTGACCCA
AGCAACACTTGAAGACATCAAGTCTCGTGAGGAGGCTGTCGGATCGTTTATCACTGTTTCAGAAGAAGTTGCTCTTAAAC
AAGCTGCGGCAATTGATGCCAAAGGTATTGATGCTGATAACCTCATGTCAGGTATTCCGTTAGCTGTTAAGGATAATATT
TCAACCAAGGAAATCCTAACCACAGCCGCTTCAAAAATGTTGTATAACTACGAGCCGATTTTTAATGCCACATCAGTAGC
AAATGCCTACGCTAAAGACATGATTGTTATTGGAAAAACCAACATGGACGAATTTGCCATGGGTGGTTCAACAGAAACCT
CTTATTTCAAGAAAACGAAAAATGCTTGGGACCACACAAAAGTACCTGGAGGCTCATCAGGTGGTTCGGCAACAGCAGTC
GCTTCAGGACAAGTCCGTTTATCTCTTGGTTCAGACACTGGTGGTTCTATTCGGCAGCCAGCTGCATTTAATGGTGTTGT
CGGTTTGAAACCAACCTATGGCACTGTTTCTCGCTATGGTCTCATTGCTTTTGGAAGTTCACTTGATCAGATTGGACCTT
TTGCTCCGACAGTTAAAGAAAACGCCCAATTATTAAATGTGATTGCAAGTAGCGATGTCAAGGATGCCACTTCAGCGCCT
GTCCGCATTGCAGACTACACATCTAAGATTGGTCGTGACATTAAAGGCATGAAGATTGCCCTTCCTAAAGAGTATCTTGG
CGAAGGGATTGATCCTGAGATTAAGGAAACGGTTCTTGCGTCAGTTAAACAATTTGAAGCCTTAGGAGCTACTGTTGAGG
AAGTCAGCCTTCCACATAGCAAGTACGGTGTGGCTGTTTATTATATTATCGCTTCGTCAGAAGCGTCTTCAAACTTACAA
CGTTTTGATGGCATTCGCTATGGTTTCCGTGCTGACGATGCTAAAAACTTGGATGAAATCTATGTTAACACTCGTAGCCA
AGGCTTTGGTGATGAGGTGAAACGTCGTATCATGCTTGGAACCTTTAGTTTGTCATCGGGTTATTATGACGCTTATTTCA
AAAAGGCCGGTCAAGTGCGCACTTTGATTATTCAAGATTTTGACAAGGTTTTTGCGGATTATGATCTTATTCTAGGCCCA
ACAACACCGACGGTTGCTTTTGGCTTAGACACACTCAACCACGATCCTGTTGCCATGTATTTGGCCGACTTATTGACCAT
TCCGGTTAACTTGGCTGGTCTGCCAGGTATTTCTATTCCAGCAGGATTCGTCGATGGCTTGCCAGTAGGGCTTCAATTAA
TTGGTCCGAAATACGCTGAAGAAACCATCTATCAAGCAGCAGCAGCCTTTGAAGCGGTGACAGATTATCACAAGCAACAA
CCGATTATTTTTGGAGGTGACAAGTAA

Upstream 100 bases:

>100_bases
AAGAAGGAACAGACCGTGCCTTGCTCTTTAAAAATGTTCCTGAAAAAGAAAATCACTTTATCAAGGTACCTGCTATTTTG
GATGACGGAGGAGATGCCTA

Downstream 100 bases:

>100_bases
TGAATTTTGAAACCATTATTGGGCTTGAAGTCCATGTGGAATTAAATACCAATTCCAAAATTTTCTCACCTTCGTCAGCT
CACTTTGGTGAGGACCCAAA

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 488; Mature: 487

Protein sequence:

>488_residues
MSFNHKTIEELHDLLVAKEISATELTQATLEDIKSREEAVGSFITVSEEVALKQAAAIDAKGIDADNLMSGIPLAVKDNI
STKEILTTAASKMLYNYEPIFNATSVANAYAKDMIVIGKTNMDEFAMGGSTETSYFKKTKNAWDHTKVPGGSSGGSATAV
ASGQVRLSLGSDTGGSIRQPAAFNGVVGLKPTYGTVSRYGLIAFGSSLDQIGPFAPTVKENAQLLNVIASSDVKDATSAP
VRIADYTSKIGRDIKGMKIALPKEYLGEGIDPEIKETVLASVKQFEALGATVEEVSLPHSKYGVAVYYIIASSEASSNLQ
RFDGIRYGFRADDAKNLDEIYVNTRSQGFGDEVKRRIMLGTFSLSSGYYDAYFKKAGQVRTLIIQDFDKVFADYDLILGP
TTPTVAFGLDTLNHDPVAMYLADLLTIPVNLAGLPGISIPAGFVDGLPVGLQLIGPKYAEETIYQAAAAFEAVTDYHKQQ
PIIFGGDK

Sequences:

>Translated_488_residues
MSFNHKTIEELHDLLVAKEISATELTQATLEDIKSREEAVGSFITVSEEVALKQAAAIDAKGIDADNLMSGIPLAVKDNI
STKEILTTAASKMLYNYEPIFNATSVANAYAKDMIVIGKTNMDEFAMGGSTETSYFKKTKNAWDHTKVPGGSSGGSATAV
ASGQVRLSLGSDTGGSIRQPAAFNGVVGLKPTYGTVSRYGLIAFGSSLDQIGPFAPTVKENAQLLNVIASSDVKDATSAP
VRIADYTSKIGRDIKGMKIALPKEYLGEGIDPEIKETVLASVKQFEALGATVEEVSLPHSKYGVAVYYIIASSEASSNLQ
RFDGIRYGFRADDAKNLDEIYVNTRSQGFGDEVKRRIMLGTFSLSSGYYDAYFKKAGQVRTLIIQDFDKVFADYDLILGP
TTPTVAFGLDTLNHDPVAMYLADLLTIPVNLAGLPGISIPAGFVDGLPVGLQLIGPKYAEETIYQAAAAFEAVTDYHKQQ
PIIFGGDK
>Mature_487_residues
SFNHKTIEELHDLLVAKEISATELTQATLEDIKSREEAVGSFITVSEEVALKQAAAIDAKGIDADNLMSGIPLAVKDNIS
TKEILTTAASKMLYNYEPIFNATSVANAYAKDMIVIGKTNMDEFAMGGSTETSYFKKTKNAWDHTKVPGGSSGGSATAVA
SGQVRLSLGSDTGGSIRQPAAFNGVVGLKPTYGTVSRYGLIAFGSSLDQIGPFAPTVKENAQLLNVIASSDVKDATSAPV
RIADYTSKIGRDIKGMKIALPKEYLGEGIDPEIKETVLASVKQFEALGATVEEVSLPHSKYGVAVYYIIASSEASSNLQR
FDGIRYGFRADDAKNLDEIYVNTRSQGFGDEVKRRIMLGTFSLSSGYYDAYFKKAGQVRTLIIQDFDKVFADYDLILGPT
TPTVAFGLDTLNHDPVAMYLADLLTIPVNLAGLPGISIPAGFVDGLPVGLQLIGPKYAEETIYQAAAAFEAVTDYHKQQP
IIFGGDK

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=506, Percent_Identity=37.3517786561265, Blast_Score=301, Evalue=1e-81,
Organism=Homo sapiens, GI195972892, Length=487, Percent_Identity=23.6139630390144, Blast_Score=110, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI17543272, Length=444, Percent_Identity=36.7117117117117, Blast_Score=258, Evalue=4e-69,
Organism=Caenorhabditis elegans, GI17537465, Length=478, Percent_Identity=22.3849372384937, Blast_Score=98, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17538252, Length=306, Percent_Identity=26.4705882352941, Blast_Score=82, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI71990152, Length=188, Percent_Identity=29.7872340425532, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17556264, Length=164, Percent_Identity=31.0975609756098, Blast_Score=73, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6323950, Length=415, Percent_Identity=33.9759036144578, Blast_Score=220, Evalue=3e-58,
Organism=Saccharomyces cerevisiae, GI6319685, Length=430, Percent_Identity=29.5348837209302, Blast_Score=122, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6320448, Length=259, Percent_Identity=27.7992277992278, Blast_Score=70, Evalue=9e-13,
Organism=Drosophila melanogaster, GI24648113, Length=490, Percent_Identity=38.7755102040816, Blast_Score=295, Evalue=6e-80,
Organism=Drosophila melanogaster, GI24652985, Length=466, Percent_Identity=24.6781115879828, Blast_Score=122, Evalue=7e-28,
Organism=Drosophila melanogaster, GI19922090, Length=466, Percent_Identity=24.6781115879828, Blast_Score=122, Evalue=7e-28,
Organism=Drosophila melanogaster, GI24652981, Length=466, Percent_Identity=24.6781115879828, Blast_Score=122, Evalue=7e-28,
Organism=Drosophila melanogaster, GI24652983, Length=466, Percent_Identity=24.6781115879828, Blast_Score=122, Evalue=7e-28,
Organism=Drosophila melanogaster, GI24644968, Length=510, Percent_Identity=23.3333333333333, Blast_Score=90, Evalue=4e-18,
Organism=Drosophila melanogaster, GI45550774, Length=208, Percent_Identity=31.25, Blast_Score=86, Evalue=4e-17,
Organism=Drosophila melanogaster, GI24648435, Length=208, Percent_Identity=31.25, Blast_Score=86, Evalue=4e-17,
Organism=Drosophila melanogaster, GI24648437, Length=208, Percent_Identity=31.25, Blast_Score=86, Evalue=4e-17,
Organism=Drosophila melanogaster, GI161078093, Length=500, Percent_Identity=22.2, Blast_Score=82, Evalue=9e-16,
Organism=Drosophila melanogaster, GI24648441, Length=138, Percent_Identity=35.5072463768116, Blast_Score=72, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24648439, Length=138, Percent_Identity=35.5072463768116, Blast_Score=72, Evalue=1e-12,
Organism=Drosophila melanogaster, GI21356731, Length=331, Percent_Identity=25.0755287009063, Blast_Score=70, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 52291; Mature: 52160

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFNHKTIEELHDLLVAKEISATELTQATLEDIKSREEAVGSFITVSEEVALKQAAAIDA
CCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHCCC
KGIDADNLMSGIPLAVKDNISTKEILTTAASKMLYNYEPIFNATSVANAYAKDMIVIGKT
CCCCHHHHHCCCCEEEECCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCEEEEECC
NMDEFAMGGSTETSYFKKTKNAWDHTKVPGGSSGGSATAVASGQVRLSLGSDTGGSIRQP
CHHHHHCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCEEEEEECCCCCCCCCCC
AAFNGVVGLKPTYGTVSRYGLIAFGSSLDQIGPFAPTVKENAQLLNVIASSDVKDATSAP
CCCCCCEECCCCCCCHHHCCCEEECCCHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCC
VRIADYTSKIGRDIKGMKIALPKEYLGEGIDPEIKETVLASVKQFEALGATVEEVSLPHS
EEEHHHHHHHCCCCCCCEEECCHHHHCCCCCHHHHHHHHHHHHHHHHHCCCHHHHCCCCC
KYGVAVYYIIASSEASSNLQRFDGIRYGFRADDAKNLDEIYVNTRSQGFGDEVKRRIMLG
CCCEEEEEEEECCCCHHHHHHHCCCCCCCCCCCCCCCHHEEEECCCCCCCHHHHHHHEEE
TFSLSSGYYDAYFKKAGQVRTLIIQDFDKVFADYDLILGPTTPTVAFGLDTLNHDPVAMY
EEECCCCCHHHHHHCCCCEEEHHHHHHHHHHCCCCEEECCCCCCEEEECCCCCCCHHHHH
LADLLTIPVNLAGLPGISIPAGFVDGLPVGLQLIGPKYAEETIYQAAAAFEAVTDYHKQQ
HHHHHHHCCCCCCCCCCCCCCHHHCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHCC
PIIFGGDK
CEEECCCC
>Mature Secondary Structure 
SFNHKTIEELHDLLVAKEISATELTQATLEDIKSREEAVGSFITVSEEVALKQAAAIDA
CCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHCCC
KGIDADNLMSGIPLAVKDNISTKEILTTAASKMLYNYEPIFNATSVANAYAKDMIVIGKT
CCCCHHHHHCCCCEEEECCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCEEEEECC
NMDEFAMGGSTETSYFKKTKNAWDHTKVPGGSSGGSATAVASGQVRLSLGSDTGGSIRQP
CHHHHHCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCEEEEEECCCCCCCCCCC
AAFNGVVGLKPTYGTVSRYGLIAFGSSLDQIGPFAPTVKENAQLLNVIASSDVKDATSAP
CCCCCCEECCCCCCCHHHCCCEEECCCHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCC
VRIADYTSKIGRDIKGMKIALPKEYLGEGIDPEIKETVLASVKQFEALGATVEEVSLPHS
EEEHHHHHHHCCCCCCCEEECCHHHHCCCCCHHHHHHHHHHHHHHHHHCCCHHHHCCCCC
KYGVAVYYIIASSEASSNLQRFDGIRYGFRADDAKNLDEIYVNTRSQGFGDEVKRRIMLG
CCCEEEEEEEECCCCHHHHHHHCCCCCCCCCCCCCCCHHEEEECCCCCCCHHHHHHHEEE
TFSLSSGYYDAYFKKAGQVRTLIIQDFDKVFADYDLILGPTTPTVAFGLDTLNHDPVAMY
EEECCCCCHHHHHHCCCCEEEHHHHHHHHHHCCCCEEECCCCCCEEEECCCCCCCHHHHH
LADLLTIPVNLAGLPGISIPAGFVDGLPVGLQLIGPKYAEETIYQAAAAFEAVTDYHKQQ
HHHHHHHCCCCCCCCCCCCCCHHHCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHCC
PIIFGGDK
CEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11296296 [H]