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The map label for this gene is polA [C]

Identifier: 71911263

GI number: 71911263

Start: 1416408

End: 1418330

Strand: Reverse

Name: polA [C]

Synonym: M5005_Spy_1450

Alternate gene names: 71911263

Gene position: 1418330-1416408 (Counterclockwise)

Preceding gene: 71911264

Following gene: 71911262

Centisome position: 77.14

GC content: 44.46

Gene sequence:

>1923_bases
ATGAGACATTTAAATATTGATATTGAAACCTATAGTTCGAATGACATCAAAAATGGGGTTTACAAGTACGCTGACGCTGA
AGATTTTGAGATTTTACTTTTCGCTTACTCTATAGATGGCGGAGAAGTAGAGTGCCTTGATTTGACAAGGCAGTCTCTAC
CTGAAGACATCAAAGATATGTTATTTGATGATAAAGTCCGAAAGCACGCCTTTAATGCCCAATTCGAAAGAGTTTGTCTC
AGTCGTTACCTCGGCCTACCTTACTATTTAGATCCCGCACAATGGCAGTGTACGATGGTACTTGCACAAGAGCTGGGTCT
GCCTGCAAGCCTGGAAAAATGTGCGCTGTATTTAAAATTAGCGCAAGAAAAAGACGCAGCAGGTAAAAATCTCATTAAAT
ATTTTTCGGTACCCTGCAAGCCTACAAAAGCCAACGGGGGAAGAACGAGGAACCTGCCCGAGCATGATCCTGGAAAATGG
CAAATGTTTATTAACTACTGTATGCAAGACGTTGTTGTTGAGATGGCAATTGCCGAAAAACTAGAGTCAGTTCCTGTGCA
CGACCGTGAGTGGGATTACTACGTCTGCGACCAGAGAATCAACGACAGAGGCGTGGCACTTGATAAAGAGTTAGTTGCTT
CGGCTTTGTATTGTAAAGATGTTAAGATGGAAAGTTTGTCTGGTGAACTAAAGGCTCTAACAGGACTTGATAATCCTAAT
AGCAGGGCGCAGCTGCTACCGTGGCTAAAAGAACACGGTTATTCGGCTAATGGGCTGACTAAAGCTGATGTTGAACAGGA
ACTTAAGAAGGCCGAAGGAGAGCTTAAGAGAGTTTTAGAACTTAAACTACAAACCGCGATGTCAAGTCTAAAAAAATATG
AAGCTATGGAAAGAGCTATGTGCTCAGACGGACGAGTTCATGGGCTACTTCAGTTTTACGGGGCTAGCCGGACAGGAAGA
TGGGCGGGCAGAGTTGTCCAAGTACAGAACTTAGCTAGGAATTATATAAAGGATCTAGATGATGCTAGAGAGTATGTTAA
AAAGCGTGATATTGATGCTGTGGAGATTTTATACGATAGCCTTAACGACACTTTAAAGCAGCTCGTGCGAACGGCACTCG
TGGCTAAAGATGGCTATACCTTCTATGTCTCTGACTTCTCGGCGATTGAGGCTAGGGTGATTGCATGGTTTGCTGGAGAG
CAGTGGAGGCTTGACGTGTTTTCTACGCACGGGAAAATCTACGAGGCGTCTGCCAGTCAGATGTTCGGAATTCCAATTGA
GGAGATTGACAAGGAACTACGCCAAAAGGGCAAAATTTCAGAGTTAGCACTTGGCTATCAAGGAGGTCCTGGAGCGCTTA
AGCAGATGGGGGCTCTAAATATGGGAGTCAAGGAAGAGGAGCTTCAAGGGTTAGTTGATGACTGGCGCAGGGCCAATAAG
AAAATCGTCCAATTTTGGAAAGACGTACAAAGAGCCGCCATCAAAGCCATCAAATCGAGAGCACCGATAAAACTTGGAAA
AATACGATTTAGATACCGGAAAGGTTTCCTCTTTATAACATTGCCTAGCGGTAGGAACTTAGCTTATGCAAGAGCCAAGG
TTGAGCCAGGCGACTATGGAGACAAAATCATCTATGAGGGCCAAGGAGATAGGGCCTATTTCACAGCGCAAGAGACGTAC
GGCGGTAAGCTTGTCGAAAATATCGTTCAGGCGACGGCCAGGGATATTCTAGCTGAAGCGCTTCTGAGGATTGAAGCTGC
AGGCTATGATGTTGTTTTCCACGTTCATGATGAGGCTATTATCGAAGGTTCAGGCCTGATAATCGAAGAGGTTAATGATT
TGATGGCTCAGGCTCCTGAATGGGCGGAGGGTCTTCCTTTAAATAGCGAAGGCTATGTAACAAAGTATTATATGAAGGAT
TAG

Upstream 100 bases:

>100_bases
AGCTGATGCAGACTTTGACGAGTGGAACGAAGAAGAGGACGAAGACGACATTTTATAGTAGAGGGCCTCTTTAGAGGCTC
CTCATTTTTAAAGGAGAGGT

Downstream 100 bases:

>100_bases
ATAGATGAAGCAAGAAAAACTAATAGTAAAGTCTTCTCCCCTGCAAGAGCTTCATATCGCAACAGGTAGTTCGCGAACAG
CTAAGACATGGAAAAATATC

Product: phage-encoded DNA polymerase

Products: N Pyrophosphate; DNA(N). [C]

Alternate protein names: DNA Polymerase; DNA Polymerase I; DNA Polymerase Bacteriophage Origin; Phage-Related DNA Polymerase; Phage Related DNA Polymerase; Family A DNA-Dependent DNA Polymerase; Prophage DNA Polymerase; DNA Polymerase I Domain Protein; Truncated Phage-Related DNA Polymerase; DNA-Directed Dna Polymerase I; DNA-Dependent DNA Polymerase Family A; DNA Polymerase I Superfamily; APSE-2 Prophage; Prophage; DNA-Directed DNA Polymerase I

Number of amino acids: Translated: 640; Mature: 640

Protein sequence:

>640_residues
MRHLNIDIETYSSNDIKNGVYKYADAEDFEILLFAYSIDGGEVECLDLTRQSLPEDIKDMLFDDKVRKHAFNAQFERVCL
SRYLGLPYYLDPAQWQCTMVLAQELGLPASLEKCALYLKLAQEKDAAGKNLIKYFSVPCKPTKANGGRTRNLPEHDPGKW
QMFINYCMQDVVVEMAIAEKLESVPVHDREWDYYVCDQRINDRGVALDKELVASALYCKDVKMESLSGELKALTGLDNPN
SRAQLLPWLKEHGYSANGLTKADVEQELKKAEGELKRVLELKLQTAMSSLKKYEAMERAMCSDGRVHGLLQFYGASRTGR
WAGRVVQVQNLARNYIKDLDDAREYVKKRDIDAVEILYDSLNDTLKQLVRTALVAKDGYTFYVSDFSAIEARVIAWFAGE
QWRLDVFSTHGKIYEASASQMFGIPIEEIDKELRQKGKISELALGYQGGPGALKQMGALNMGVKEEELQGLVDDWRRANK
KIVQFWKDVQRAAIKAIKSRAPIKLGKIRFRYRKGFLFITLPSGRNLAYARAKVEPGDYGDKIIYEGQGDRAYFTAQETY
GGKLVENIVQATARDILAEALLRIEAAGYDVVFHVHDEAIIEGSGLIIEEVNDLMAQAPEWAEGLPLNSEGYVTKYYMKD

Sequences:

>Translated_640_residues
MRHLNIDIETYSSNDIKNGVYKYADAEDFEILLFAYSIDGGEVECLDLTRQSLPEDIKDMLFDDKVRKHAFNAQFERVCL
SRYLGLPYYLDPAQWQCTMVLAQELGLPASLEKCALYLKLAQEKDAAGKNLIKYFSVPCKPTKANGGRTRNLPEHDPGKW
QMFINYCMQDVVVEMAIAEKLESVPVHDREWDYYVCDQRINDRGVALDKELVASALYCKDVKMESLSGELKALTGLDNPN
SRAQLLPWLKEHGYSANGLTKADVEQELKKAEGELKRVLELKLQTAMSSLKKYEAMERAMCSDGRVHGLLQFYGASRTGR
WAGRVVQVQNLARNYIKDLDDAREYVKKRDIDAVEILYDSLNDTLKQLVRTALVAKDGYTFYVSDFSAIEARVIAWFAGE
QWRLDVFSTHGKIYEASASQMFGIPIEEIDKELRQKGKISELALGYQGGPGALKQMGALNMGVKEEELQGLVDDWRRANK
KIVQFWKDVQRAAIKAIKSRAPIKLGKIRFRYRKGFLFITLPSGRNLAYARAKVEPGDYGDKIIYEGQGDRAYFTAQETY
GGKLVENIVQATARDILAEALLRIEAAGYDVVFHVHDEAIIEGSGLIIEEVNDLMAQAPEWAEGLPLNSEGYVTKYYMKD
>Mature_640_residues
MRHLNIDIETYSSNDIKNGVYKYADAEDFEILLFAYSIDGGEVECLDLTRQSLPEDIKDMLFDDKVRKHAFNAQFERVCL
SRYLGLPYYLDPAQWQCTMVLAQELGLPASLEKCALYLKLAQEKDAAGKNLIKYFSVPCKPTKANGGRTRNLPEHDPGKW
QMFINYCMQDVVVEMAIAEKLESVPVHDREWDYYVCDQRINDRGVALDKELVASALYCKDVKMESLSGELKALTGLDNPN
SRAQLLPWLKEHGYSANGLTKADVEQELKKAEGELKRVLELKLQTAMSSLKKYEAMERAMCSDGRVHGLLQFYGASRTGR
WAGRVVQVQNLARNYIKDLDDAREYVKKRDIDAVEILYDSLNDTLKQLVRTALVAKDGYTFYVSDFSAIEARVIAWFAGE
QWRLDVFSTHGKIYEASASQMFGIPIEEIDKELRQKGKISELALGYQGGPGALKQMGALNMGVKEEELQGLVDDWRRANK
KIVQFWKDVQRAAIKAIKSRAPIKLGKIRFRYRKGFLFITLPSGRNLAYARAKVEPGDYGDKIIYEGQGDRAYFTAQETY
GGKLVENIVQATARDILAEALLRIEAAGYDVVFHVHDEAIIEGSGLIIEEVNDLMAQAPEWAEGLPLNSEGYVTKYYMKD

Specific function: In Addition To Polymerase Activity, This DNA Polymerase Exhibits 3' To 5' And 5' To 3' Exonuclease Activity. It Is Able To Utilize Nicked Circular Duplex DNA As A Template And Can Unwind The Parental DNA Strand From Its Template. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 2.7.7.7 [C]

Molecular weight: Translated: 72517; Mature: 72517

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: PS00447 DNA_POLYMERASE_A

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRHLNIDIETYSSNDIKNGVYKYADAEDFEILLFAYSIDGGEVECLDLTRQSLPEDIKDM
CCEEEEEEEECCCCCHHCCCEECCCCCCCEEEEEEEECCCCCEEEEHHHHHHCCHHHHHH
LFDDKVRKHAFNAQFERVCLSRYLGLPYYLDPAQWQCTMVLAQELGLPASLEKCALYLKL
HHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHCCCCCCHHHHHHHHHH
AQEKDAAGKNLIKYFSVPCKPTKANGGRTRNLPEHDPGKWQMFINYCMQDVVVEMAIAEK
HHHCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
LESVPVHDREWDYYVCDQRINDRGVALDKELVASALYCKDVKMESLSGELKALTGLDNPN
HHCCCCCCCCCCEEEECCCCCCCCCEECHHHHHHHHHHHCCHHHHHCCCHHHEECCCCCC
SRAQLLPWLKEHGYSANGLTKADVEQELKKAEGELKRVLELKLQTAMSSLKKYEAMERAM
CHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CSDGRVHGLLQFYGASRTGRWAGRVVQVQNLARNYIKDLDDAREYVKKRDIDAVEILYDS
HCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
LNDTLKQLVRTALVAKDGYTFYVSDFSAIEARVIAWFAGEQWRLDVFSTHGKIYEASASQ
HHHHHHHHHHHHHHHCCCCEEEEECHHHHHHEEEEEECCCCEEEEEEECCCCEEECCCHH
MFGIPIEEIDKELRQKGKISELALGYQGGPGALKQMGALNMGVKEEELQGLVDDWRRANK
CCCCCHHHHHHHHHHCCCHHHHEECCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
KIVQFWKDVQRAAIKAIKSRAPIKLGKIRFRYRKGFLFITLPSGRNLAYARAKVEPGDYG
HHHHHHHHHHHHHHHHHHHCCCEEEHHEEEEEECCEEEEECCCCCCEEEEEEECCCCCCC
DKIIYEGQGDRAYFTAQETYGGKLVENIVQATARDILAEALLRIEAAGYDVVFHVHDEAI
CEEEEECCCCEEEEEECHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCEE
IEGSGLIIEEVNDLMAQAPEWAEGLPLNSEGYVTKYYMKD
EECCCCHHHHHHHHHHCCCCHHCCCCCCCCCCEEEEEECC
>Mature Secondary Structure
MRHLNIDIETYSSNDIKNGVYKYADAEDFEILLFAYSIDGGEVECLDLTRQSLPEDIKDM
CCEEEEEEEECCCCCHHCCCEECCCCCCCEEEEEEEECCCCCEEEEHHHHHHCCHHHHHH
LFDDKVRKHAFNAQFERVCLSRYLGLPYYLDPAQWQCTMVLAQELGLPASLEKCALYLKL
HHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHCCCCCCHHHHHHHHHH
AQEKDAAGKNLIKYFSVPCKPTKANGGRTRNLPEHDPGKWQMFINYCMQDVVVEMAIAEK
HHHCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
LESVPVHDREWDYYVCDQRINDRGVALDKELVASALYCKDVKMESLSGELKALTGLDNPN
HHCCCCCCCCCCEEEECCCCCCCCCEECHHHHHHHHHHHCCHHHHHCCCHHHEECCCCCC
SRAQLLPWLKEHGYSANGLTKADVEQELKKAEGELKRVLELKLQTAMSSLKKYEAMERAM
CHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CSDGRVHGLLQFYGASRTGRWAGRVVQVQNLARNYIKDLDDAREYVKKRDIDAVEILYDS
HCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
LNDTLKQLVRTALVAKDGYTFYVSDFSAIEARVIAWFAGEQWRLDVFSTHGKIYEASASQ
HHHHHHHHHHHHHHHCCCCEEEEECHHHHHHEEEEEECCCCEEEEEEECCCCEEECCCHH
MFGIPIEEIDKELRQKGKISELALGYQGGPGALKQMGALNMGVKEEELQGLVDDWRRANK
CCCCCHHHHHHHHHHCCCHHHHEECCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
KIVQFWKDVQRAAIKAIKSRAPIKLGKIRFRYRKGFLFITLPSGRNLAYARAKVEPGDYG
HHHHHHHHHHHHHHHHHHHCCCEEEHHEEEEEECCEEEEECCCCCCEEEEEEECCCCCCC
DKIIYEGQGDRAYFTAQETYGGKLVENIVQATARDILAEALLRIEAAGYDVVFHVHDEAI
CEEEEECCCCEEEEEECHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCEE
IEGSGLIIEEVNDLMAQAPEWAEGLPLNSEGYVTKYYMKD
EECCCCHHHHHHHHHHCCCCHHCCCCCCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Dimethyl sulfoxide epsilon,; Polymerase alpha accessory factors; Proliferating cell nuclear antigen; Replication factor A; Replication factor C; Thiol [C]

Metal ions: K+; Mg2+; Mn2+; Na+; Zn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0063 {dATP}} [C]

Substrates: N Deoxynucleoside Triphosphate; DNA [C]

Specific reaction: N Deoxynucleoside Triphosphate = N Pyrophosphate + DNA(N). Protein + DNA = Protein-DNA [C]

General reaction: Nucleotidyl group transfer [C]

Inhibitor: 1, 10-Phenanthroline; 2', 3'-Dideoxythymidine5'-triphosphate polymerase beta, gamma, delta, epsilon; Aphidicolin polymerase alpha; Ara-ATP; Ara-CTP; Benzyl oxycarbonyl -Leu-Leu-al; Carbonyl diphosphonate delta, alpha,; Dideoxynucleoside 5'-triphosphate;

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA