Click here to switch to the map view.

The map label for this gene is mboIBM [H]

Identifier: 71911257

GI number: 71911257

Start: 1410747

End: 1411598

Strand: Reverse

Name: mboIBM [H]

Synonym: M5005_Spy_1444

Alternate gene names: 71911257

Gene position: 1411598-1410747 (Counterclockwise)

Preceding gene: 71911258

Following gene: 71911256

Centisome position: 76.78

GC content: 38.38

Gene sequence:

>852_bases
ATGGAAATAAAATGCGGAAATTGCTTAGAATTACTAGCGGAGTTAACTGATGGATTGATCGACATGGTGATAACGGACCC
ACCATACAACATATCAGTAAAAAATAACTTTGCTACCATGGGGCGAACAGGTATAGATTTTGGTGATTGGGATAAAGGTT
TTGATTTACTGAGTTGGATTGACATTGCAAGTCAAAAATTAACTAAAAACGGTGGCATGATAATCTTCAACGATTGGAAA
AATTTAGGAGATATTGCAAGACATTGTGAAAAAAATGGGTTAGTAGTAAAGGATATTTTTAGGTGGGTAAAAGATAACCC
TATGCCCAGAAACAGAGATAGAAGGTATATTACAGATAGCGAGTACGGGGTATGGGTCGTCCGAAAAAAATCCAAGTGGG
TATTTAATCGTAAATCTGAAAAATACGATAGGCCAGAATACAGATATCCAGTTGTGGCAGGAGCAGAAAAAACACAGCAT
CCGACACAAAAGCCAGTAGCTCTGATGAAGGACATCATTACGCGACACACAACAAAAGGGGCTGTTGTCCTAGATCCTTT
TATGGGCAGCGGATCAACAGGAGTAGCTTGTCTACTGACGGGGAGAGACTTTATTGGATATGAGCTAAATAAAGATTACT
TTAATATCGCAAACAAACGGATTGCAGAATTGACAGGAAATGGGGGGGTAGGTATGAATATAATCCATAGAATGGAAAAC
GGGATAGATGCTGTAGGTGATTTAACAACTTATGCAGTACCATGTTGTCCAGAGTGTAAAAATGAAACATATAGTCAGAA
ACACTGTCCTTTTTGCGGTGAAAATCTTATGGAGGTAGCAAATGAAAATTGA

Upstream 100 bases:

>100_bases
TAAGCAGGACACAAAAGCAGCTTAAACGTGCGAGTGAGGATAGAGCTAGACAGACAAAACGGATTGCGGAATTGACAGGG
AATGGGGGGTAAGAAAACAC

Downstream 100 bases:

>100_bases
ATTTTTTTTAAAGCAAGGTCCTACTAAGAGCGTTTCTAATTATGGTGAAATTTGTATTGATGAAGTAATTGGCGCAATGA
AAAATAACGATGTCATTGCT

Product: adenine-specific methyltransferase

Products: NA

Alternate protein names: M.MboIB; Adenine-specific methyltransferase MboIB; M.MboI C [H]

Number of amino acids: Translated: 283; Mature: 283

Protein sequence:

>283_residues
MEIKCGNCLELLAELTDGLIDMVITDPPYNISVKNNFATMGRTGIDFGDWDKGFDLLSWIDIASQKLTKNGGMIIFNDWK
NLGDIARHCEKNGLVVKDIFRWVKDNPMPRNRDRRYITDSEYGVWVVRKKSKWVFNRKSEKYDRPEYRYPVVAGAEKTQH
PTQKPVALMKDIITRHTTKGAVVLDPFMGSGSTGVACLLTGRDFIGYELNKDYFNIANKRIAELTGNGGVGMNIIHRMEN
GIDAVGDLTTYAVPCCPECKNETYSQKHCPFCGENLMEVANEN

Sequences:

>Translated_283_residues
MEIKCGNCLELLAELTDGLIDMVITDPPYNISVKNNFATMGRTGIDFGDWDKGFDLLSWIDIASQKLTKNGGMIIFNDWK
NLGDIARHCEKNGLVVKDIFRWVKDNPMPRNRDRRYITDSEYGVWVVRKKSKWVFNRKSEKYDRPEYRYPVVAGAEKTQH
PTQKPVALMKDIITRHTTKGAVVLDPFMGSGSTGVACLLTGRDFIGYELNKDYFNIANKRIAELTGNGGVGMNIIHRMEN
GIDAVGDLTTYAVPCCPECKNETYSQKHCPFCGENLMEVANEN
>Mature_283_residues
MEIKCGNCLELLAELTDGLIDMVITDPPYNISVKNNFATMGRTGIDFGDWDKGFDLLSWIDIASQKLTKNGGMIIFNDWK
NLGDIARHCEKNGLVVKDIFRWVKDNPMPRNRDRRYITDSEYGVWVVRKKSKWVFNRKSEKYDRPEYRYPVVAGAEKTQH
PTQKPVALMKDIITRHTTKGAVVLDPFMGSGSTGVACLLTGRDFIGYELNKDYFNIANKRIAELTGNGGVGMNIIHRMEN
GIDAVGDLTTYAVPCCPECKNETYSQKHCPFCGENLMEVANEN

Specific function: This methylase recognizes the double-stranded sequence GATC, causes specific methylation on A-2 on both strands, and protects the DNA from cleavage by the MboI endonuclease [H]

COG id: COG0863

COG function: function code L; DNA modification methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI87082238, Length=249, Percent_Identity=28.9156626506024, Blast_Score=86, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002941
- InterPro:   IPR002052
- InterPro:   IPR001091 [H]

Pfam domain/function: PF01555 N6_N4_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 31992; Mature: 31992

Theoretical pI: Translated: 7.34; Mature: 7.34

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
6.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEIKCGNCLELLAELTDGLIDMVITDPPYNISVKNNFATMGRTGIDFGDWDKGFDLLSWI
CCCCCHHHHHHHHHHHHHHEEEEECCCCCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHH
DIASQKLTKNGGMIIFNDWKNLGDIARHCEKNGLVVKDIFRWVKDNPMPRNRDRRYITDS
HHHHHHHHCCCCEEEECCCCCHHHHHHHHHCCCEEHHHHHHHHHCCCCCCCCCCCEEECC
EYGVWVVRKKSKWVFNRKSEKYDRPEYRYPVVAGAEKTQHPTQKPVALMKDIITRHTTKG
CCCEEEEEECCCEEECCCCCCCCCCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHCCCCC
AVVLDPFMGSGSTGVACLLTGRDFIGYELNKDYFNIANKRIAELTGNGGVGMNIIHRMEN
EEEECCCCCCCCCCEEEEEECCCCCCEECCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
GIDAVGDLTTYAVPCCPECKNETYSQKHCPFCGENLMEVANEN
CHHHHHHHHHHCCCCCCHHCCCCCCCCCCCCCHHHHHHHHCCC
>Mature Secondary Structure
MEIKCGNCLELLAELTDGLIDMVITDPPYNISVKNNFATMGRTGIDFGDWDKGFDLLSWI
CCCCCHHHHHHHHHHHHHHEEEEECCCCCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHH
DIASQKLTKNGGMIIFNDWKNLGDIARHCEKNGLVVKDIFRWVKDNPMPRNRDRRYITDS
HHHHHHHHCCCCEEEECCCCCHHHHHHHHHCCCEEHHHHHHHHHCCCCCCCCCCCEEECC
EYGVWVVRKKSKWVFNRKSEKYDRPEYRYPVVAGAEKTQHPTQKPVALMKDIITRHTTKG
CCCEEEEEECCCEEECCCCCCCCCCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHCCCCC
AVVLDPFMGSGSTGVACLLTGRDFIGYELNKDYFNIANKRIAELTGNGGVGMNIIHRMEN
EEEECCCCCCCCCCEEEEEECCCCCCEECCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
GIDAVGDLTTYAVPCCPECKNETYSQKHCPFCGENLMEVANEN
CHHHHHHHHHHCCCCCCHHCCCCCCCCCCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8506128 [H]