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The map label for this gene is glpF [H]

Identifier: 71911192

GI number: 71911192

Start: 1350848

End: 1351549

Strand: Reverse

Name: glpF [H]

Synonym: M5005_Spy_1379

Alternate gene names: 71911192

Gene position: 1351549-1350848 (Counterclockwise)

Preceding gene: 71911193

Following gene: 71911191

Centisome position: 73.51

GC content: 46.44

Gene sequence:

>702_bases
ATGGATATCTTTGGCGAATTTTTGGGTACAGCCTTATTGGTTTTACTAGGAAATGGTGTGGTAGCAGGGGTTGTCTTGCC
CAAAACGAAAACCCATGCTTCTGGCTGGATCGTGATTGCGACTGGCTGGGGAATTGCAGTTGCTGTGGCAGCCTTTATCA
GTGGCAAAGTTGCCCCTGCCCACCTCAATCCAGCTGTTAGCCTTGCTTTTGCCATGAGTGGAACCATTGCCTGGTCAACA
GCCATTGCCTATAGCCTTGCCCAACTATTGGGAGCTATGGTTGGTTCAACTCTGGTATTCCTTCAGTTCAGGCCACATTA
TCTGGCTGCTGAGAGTCAGGCTGATATTTTAGGGACATTTGCGACAGGTCCTGCTATTCGAGATACTAGCTCAAACCTAT
TGAGTGAAATCTTTGGGACCTTTGTCTTGATGCTTGGTATTTTGGCATTTGGCTTATATGATATGCCAGCAGGACTAGGA
ACCCTCTGTGTAGGTACCTTGGTTATTGGGATTGGGTTATCCTTAGGAGGAACGACAGGTTACGCCATTAACCCAGCACG
TGATTTAGGACCTCGTCTAGTTCATGCTATTCTACCACTCAACAACAAGGGAGATTCTGATTGGTCTTATGCTTGGATAC
CTGTTGTAGGACCAATTATTGGAGCGGTCTTAGCAGTTTTGCTTTTCCAAGTCATGTCCTAA

Upstream 100 bases:

>100_bases
GAATGGTCTGATCAGGAAAGAGTGGCACAAGAAGACGATCTTCGTCGCGTGATTGCAGACAATGATTTGAGTGCCTTAAA
AGGCCATCAGGAGGGTTAAT

Downstream 100 bases:

>100_bases
CCATCACTAAAGGGGTTGGCTATCATAGGCCAGCTCCTTTGCATTTTCTTTATAGGATTAGGTAAAACGCTTTCATATCT
TAAAAAAAACTGCTATAATA

Product: glycerol uptake facilitator protein

Products: glycerol [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 233; Mature: 233

Protein sequence:

>233_residues
MDIFGEFLGTALLVLLGNGVVAGVVLPKTKTHASGWIVIATGWGIAVAVAAFISGKVAPAHLNPAVSLAFAMSGTIAWST
AIAYSLAQLLGAMVGSTLVFLQFRPHYLAAESQADILGTFATGPAIRDTSSNLLSEIFGTFVLMLGILAFGLYDMPAGLG
TLCVGTLVIGIGLSLGGTTGYAINPARDLGPRLVHAILPLNNKGDSDWSYAWIPVVGPIIGAVLAVLLFQVMS

Sequences:

>Translated_233_residues
MDIFGEFLGTALLVLLGNGVVAGVVLPKTKTHASGWIVIATGWGIAVAVAAFISGKVAPAHLNPAVSLAFAMSGTIAWST
AIAYSLAQLLGAMVGSTLVFLQFRPHYLAAESQADILGTFATGPAIRDTSSNLLSEIFGTFVLMLGILAFGLYDMPAGLG
TLCVGTLVIGIGLSLGGTTGYAINPARDLGPRLVHAILPLNNKGDSDWSYAWIPVVGPIIGAVLAVLLFQVMS
>Mature_233_residues
MDIFGEFLGTALLVLLGNGVVAGVVLPKTKTHASGWIVIATGWGIAVAVAAFISGKVAPAHLNPAVSLAFAMSGTIAWST
AIAYSLAQLLGAMVGSTLVFLQFRPHYLAAESQADILGTFATGPAIRDTSSNLLSEIFGTFVLMLGILAFGLYDMPAGLG
TLCVGTLVIGIGLSLGGTTGYAINPARDLGPRLVHAILPLNNKGDSDWSYAWIPVVGPIIGAVLAVLLFQVMS

Specific function: Glycerol enters the cell via the glycerol diffusion facilitator protein. This membrane protein facilitates the movement of glycerol across the cytoplasmic membrane [H]

COG id: COG0580

COG function: function code G; Glycerol uptake facilitator and related permeases (Major Intrinsic Protein Family)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MIP/aquaporin (TC 1.A.8) family [H]

Homologues:

Organism=Homo sapiens, GI157266307, Length=245, Percent_Identity=36.734693877551, Blast_Score=123, Evalue=1e-28,
Organism=Homo sapiens, GI4826645, Length=242, Percent_Identity=36.7768595041322, Blast_Score=120, Evalue=7e-28,
Organism=Homo sapiens, GI22538420, Length=246, Percent_Identity=33.739837398374, Blast_Score=108, Evalue=3e-24,
Organism=Homo sapiens, GI4502187, Length=245, Percent_Identity=31.8367346938775, Blast_Score=96, Evalue=2e-20,
Organism=Homo sapiens, GI4755125, Length=199, Percent_Identity=28.643216080402, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI4502181, Length=199, Percent_Identity=28.643216080402, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI37694062, Length=235, Percent_Identity=28.936170212766, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI4502179, Length=237, Percent_Identity=32.9113924050633, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI86792455, Length=233, Percent_Identity=29.1845493562232, Blast_Score=65, Evalue=5e-11,
Organism=Homo sapiens, GI4502183, Length=232, Percent_Identity=29.7413793103448, Blast_Score=64, Evalue=8e-11,
Organism=Escherichia coli, GI1790362, Length=246, Percent_Identity=33.739837398374, Blast_Score=97, Evalue=8e-22,
Organism=Caenorhabditis elegans, GI71994009, Length=229, Percent_Identity=32.7510917030568, Blast_Score=111, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI17531429, Length=240, Percent_Identity=32.9166666666667, Blast_Score=110, Evalue=9e-25,
Organism=Caenorhabditis elegans, GI17531431, Length=240, Percent_Identity=32.9166666666667, Blast_Score=110, Evalue=9e-25,
Organism=Caenorhabditis elegans, GI17544068, Length=244, Percent_Identity=33.1967213114754, Blast_Score=101, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI71992966, Length=246, Percent_Identity=26.0162601626016, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17558372, Length=235, Percent_Identity=30.6382978723404, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI32564052, Length=235, Percent_Identity=30.2127659574468, Blast_Score=81, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17533613, Length=235, Percent_Identity=30.2127659574468, Blast_Score=81, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI71993722, Length=233, Percent_Identity=30.0429184549356, Blast_Score=64, Evalue=7e-11,
Organism=Saccharomyces cerevisiae, GI6321054, Length=216, Percent_Identity=30.0925925925926, Blast_Score=82, Evalue=5e-17,
Organism=Saccharomyces cerevisiae, GI6322985, Length=212, Percent_Identity=29.7169811320755, Blast_Score=66, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24652747, Length=233, Percent_Identity=28.3261802575107, Blast_Score=68, Evalue=6e-12,
Organism=Drosophila melanogaster, GI45551084, Length=233, Percent_Identity=28.3261802575107, Blast_Score=68, Evalue=6e-12,
Organism=Drosophila melanogaster, GI24762342, Length=239, Percent_Identity=26.7782426778243, Blast_Score=65, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012269
- InterPro:   IPR000425 [H]

Pfam domain/function: PF00230 MIP [H]

EC number: NA

Molecular weight: Translated: 23953; Mature: 23953

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00221 MIP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIFGEFLGTALLVLLGNGVVAGVVLPKTKTHASGWIVIATGWGIAVAVAAFISGKVAPA
CCHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCEEEEEECHHHHHHHHHHHCCCCCCC
HLNPAVSLAFAMSGTIAWSTAIAYSLAQLLGAMVGSTLVFLQFRPHYLAAESQADILGTF
CCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCEECCCCCCHHEEEC
ATGPAIRDTSSNLLSEIFGTFVLMLGILAFGLYDMPAGLGTLCVGTLVIGIGLSLGGTTG
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCC
YAINPARDLGPRLVHAILPLNNKGDSDWSYAWIPVVGPIIGAVLAVLLFQVMS
CEECCHHHHHHHHHHHHHCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure
MDIFGEFLGTALLVLLGNGVVAGVVLPKTKTHASGWIVIATGWGIAVAVAAFISGKVAPA
CCHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCEEEEEECHHHHHHHHHHHCCCCCCC
HLNPAVSLAFAMSGTIAWSTAIAYSLAQLLGAMVGSTLVFLQFRPHYLAAESQADILGTF
CCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCEECCCCCCHHEEEC
ATGPAIRDTSSNLLSEIFGTFVLMLGILAFGLYDMPAGLGTLCVGTLVIGIGLSLGGTTG
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCC
YAINPARDLGPRLVHAILPLNNKGDSDWSYAWIPVVGPIIGAVLAVLLFQVMS
CEECCHHHHHHHHHHHHHCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: glycerol [Periplasm] [C]

Specific reaction: glycerol [Periplasm] = glycerol [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7565084; 11463916 [H]