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The map label for this gene is comEC [H]

Identifier: 71910960

GI number: 71910960

Start: 1123112

End: 1125355

Strand: Reverse

Name: comEC [H]

Synonym: M5005_Spy_1147

Alternate gene names: 71910960

Gene position: 1125355-1123112 (Counterclockwise)

Preceding gene: 71910961

Following gene: 71910959

Centisome position: 61.21

GC content: 39.48

Gene sequence:

>2244_bases
ATGACATTTTCTTGGACTAAGCTAGTGCCCCTGTCAAAGATTCAATTTGCTTTTTTAATCTTGGTTTTCTTTTATCAAAT
CCATAGCCCTAGCTGGTTAACGTTTCTCCTTAGCCTTAGTTTGATTTGCTTACTTGTCAAGCGTTTGTCTAAAAAAGAAT
TTCTCGGTGTCTTTGCCATCCTTAGTTTCTGTGCTTTGTTTTTGCTGTATCAGAAACAGCAGTTGGTTCAAAAACTGGAA
ATTCAGCCAGTACAAATAACCTCTGTAGCATTAGTTCCTGATAGTATTCGGATTAATGGTGATCAATTAGCTGTTCTTGG
ACGTCACGGGAAACATTCTTATCAGTTATTTTACCGTTTGAAAAGCCAAGCAGAAGCCCAATTGTTCAAGAAAGAGCACC
GTTGGTTAGTGATGCACGCAAAAGTGACATTGGAAAAAGCTGAGGAAGTTCGAAATTTTAAAGGCTTTAATTATCAGACT
TTTTTGACTTATCAAGGAATTTACAGGATAGGGAAGGTAGAGCAAATAGAACAGCTTGAAGTGATTTCACCCGAGTCAAT
ATGTGACTATTTGTCCTCTTTAAGACGACGAGCCATTGTTCATTGCCAGCAGCATTTTCCAAGACCTATGTCTCACTACC
TGACGGGCCTCCTTTTTGGTTATTTAGATAAGTCGTTTGGTGAGATGACAGATTATTATAGTCAGTTGGGTATCATTCAT
CTCTTTGCTTTATCAGGGATGCAGGTTGGCTTCTTTCTCACTTGTTTTAGAAGGGTGTTGCTATTACTCGCAGTTCCTTT
GGAATGGATAAAATGGATAGAGCTTCCTTTTGCTTGCTTTTATGCCGCGTTGACAGGTTATAGTATTTCTGTTATTCGAA
GCCTCGTGCAGTCGCAGTTGCGTCATTTAGGAATAAAAGGATTAGACAATCTGGCTTGTACTTTTTTACTTGTCTTTTTA
TGGGATGCTCATTTCTTAATGACTGTTGGTGGCGTATTAACGTTTAGCTATGCTTTTTTGTTAACAGTGGTTACTGTTGA
AGAATTATCAGGAGCTAAAAGACAGTTGGTACAAGTCTTGACCATTTCCCTAGGAATATTACCATTTTTGCTCTTTTATT
TTTCCAGTTTTAACCCAATGTCAATGGTCTTAACAGGGCTCTTGTCTTATCTCTTTGACCTTTTTATTTTGCCCCTTCTT
TGTCTGGTTTTTTGTCTATCCCCTTTGGTGACTGTCTCAATATGTAACCATCTTTTTATTTTATTAGAAAAAGTCATTCA
ATTTCTCGGAAATACTTTCAATAGTTCCCTTGTTTTTGGAAGCCCGACCAGTTGGCACTTGCTTATCTTGGTGATTAGCT
TCGCTATTTTTTATGATTATCGGCAGGTGCGACAACGAGTTATCACTTGCGGTTTGGTTATAGCGTTGACACTTTTGTCT
GTCAAATATCCTTTGACCAATGAAGTGACCTTTATCGACATTGGCCAGGGAGATAGTATTTTGGTGAGGGAGTGGACAGG
GAAAAATCTCTTAATAGATGTAGGTGGGCGCCCTTTCTTTTCTTCAAAAGAGCACTGGCGACGAGGACATCACGTAGCTA
ATGCTCAGAAAACCTTAATTCCTTACTTAAAAAGTAGAGGTATTCACACAATTGACCAACTATTAGTAACTCACGCAGAT
ACTGATCATATGGGGGATATCGAAGTGGTTGCCAAAGCTATTCGGATAAAGGAGATTTTGACTAGTCAAGGCAGTTTAAG
CCATCCAAGCTTTGTTAGGCGGCTAAGGCGTTTAAAATGTCACGTTAGGGTTTTAGCAGCTGGAGACCAGTTGCCTATTA
TGGGAAGTGTCTTACAGGTACTCTACCCTTGGCAGTTAGGGGACGGGAAAAATAACGATTCTTTGGTACTTTATGGCAGG
CTCTTAAATCGAACTTTTCTTTTCACAGGTGACTTGGAAAAAGAGGGGGAAAACGAGATCATAAAACGTTATCCTCAACT
AAGAGTAGATTATTTAAAAGCAGGTCACCATGGATCAAATACCTCTTCTAGTGCAGCTTTTTTAGACCATATTCAGCCTA
AAGTGGCCTTTATTTCTGCTGGAAAAAATAACCGTTATCAGCATCCTCACAGAGAAACCTTAGCCCGTTTGGAAGACAGA
CAAATCACTTATTACCGAACGGATACGCAAGGAGCTATTCGTTTGACTGGTCGGACAAGCTGGCATCTTGAAACGGTTCG
TTAG

Upstream 100 bases:

>100_bases
GATATTATTGATATGAGAGATAAGTTAGGTGGTTTTAAAGCGTTAGAAGATCTCCGTCAGGTTTCGGGGATTGGCGAAAA
AACGCTCGAGAAATTAAAAG

Downstream 100 bases:

>100_bases
TTAAAATTCCAGCCTGACGAAGAAACAAGCAGTCGCTTTCCTTTTAGGAGAAAGATGATATAATAAAAGCATGATTGCGA
TAGAAAAGATTGAAAAACTG

Product: COME operon protein 3

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 747; Mature: 746

Protein sequence:

>747_residues
MTFSWTKLVPLSKIQFAFLILVFFYQIHSPSWLTFLLSLSLICLLVKRLSKKEFLGVFAILSFCALFLLYQKQQLVQKLE
IQPVQITSVALVPDSIRINGDQLAVLGRHGKHSYQLFYRLKSQAEAQLFKKEHRWLVMHAKVTLEKAEEVRNFKGFNYQT
FLTYQGIYRIGKVEQIEQLEVISPESICDYLSSLRRRAIVHCQQHFPRPMSHYLTGLLFGYLDKSFGEMTDYYSQLGIIH
LFALSGMQVGFFLTCFRRVLLLLAVPLEWIKWIELPFACFYAALTGYSISVIRSLVQSQLRHLGIKGLDNLACTFLLVFL
WDAHFLMTVGGVLTFSYAFLLTVVTVEELSGAKRQLVQVLTISLGILPFLLFYFSSFNPMSMVLTGLLSYLFDLFILPLL
CLVFCLSPLVTVSICNHLFILLEKVIQFLGNTFNSSLVFGSPTSWHLLILVISFAIFYDYRQVRQRVITCGLVIALTLLS
VKYPLTNEVTFIDIGQGDSILVREWTGKNLLIDVGGRPFFSSKEHWRRGHHVANAQKTLIPYLKSRGIHTIDQLLVTHAD
TDHMGDIEVVAKAIRIKEILTSQGSLSHPSFVRRLRRLKCHVRVLAAGDQLPIMGSVLQVLYPWQLGDGKNNDSLVLYGR
LLNRTFLFTGDLEKEGENEIIKRYPQLRVDYLKAGHHGSNTSSSAAFLDHIQPKVAFISAGKNNRYQHPHRETLARLEDR
QITYYRTDTQGAIRLTGRTSWHLETVR

Sequences:

>Translated_747_residues
MTFSWTKLVPLSKIQFAFLILVFFYQIHSPSWLTFLLSLSLICLLVKRLSKKEFLGVFAILSFCALFLLYQKQQLVQKLE
IQPVQITSVALVPDSIRINGDQLAVLGRHGKHSYQLFYRLKSQAEAQLFKKEHRWLVMHAKVTLEKAEEVRNFKGFNYQT
FLTYQGIYRIGKVEQIEQLEVISPESICDYLSSLRRRAIVHCQQHFPRPMSHYLTGLLFGYLDKSFGEMTDYYSQLGIIH
LFALSGMQVGFFLTCFRRVLLLLAVPLEWIKWIELPFACFYAALTGYSISVIRSLVQSQLRHLGIKGLDNLACTFLLVFL
WDAHFLMTVGGVLTFSYAFLLTVVTVEELSGAKRQLVQVLTISLGILPFLLFYFSSFNPMSMVLTGLLSYLFDLFILPLL
CLVFCLSPLVTVSICNHLFILLEKVIQFLGNTFNSSLVFGSPTSWHLLILVISFAIFYDYRQVRQRVITCGLVIALTLLS
VKYPLTNEVTFIDIGQGDSILVREWTGKNLLIDVGGRPFFSSKEHWRRGHHVANAQKTLIPYLKSRGIHTIDQLLVTHAD
TDHMGDIEVVAKAIRIKEILTSQGSLSHPSFVRRLRRLKCHVRVLAAGDQLPIMGSVLQVLYPWQLGDGKNNDSLVLYGR
LLNRTFLFTGDLEKEGENEIIKRYPQLRVDYLKAGHHGSNTSSSAAFLDHIQPKVAFISAGKNNRYQHPHRETLARLEDR
QITYYRTDTQGAIRLTGRTSWHLETVR
>Mature_746_residues
TFSWTKLVPLSKIQFAFLILVFFYQIHSPSWLTFLLSLSLICLLVKRLSKKEFLGVFAILSFCALFLLYQKQQLVQKLEI
QPVQITSVALVPDSIRINGDQLAVLGRHGKHSYQLFYRLKSQAEAQLFKKEHRWLVMHAKVTLEKAEEVRNFKGFNYQTF
LTYQGIYRIGKVEQIEQLEVISPESICDYLSSLRRRAIVHCQQHFPRPMSHYLTGLLFGYLDKSFGEMTDYYSQLGIIHL
FALSGMQVGFFLTCFRRVLLLLAVPLEWIKWIELPFACFYAALTGYSISVIRSLVQSQLRHLGIKGLDNLACTFLLVFLW
DAHFLMTVGGVLTFSYAFLLTVVTVEELSGAKRQLVQVLTISLGILPFLLFYFSSFNPMSMVLTGLLSYLFDLFILPLLC
LVFCLSPLVTVSICNHLFILLEKVIQFLGNTFNSSLVFGSPTSWHLLILVISFAIFYDYRQVRQRVITCGLVIALTLLSV
KYPLTNEVTFIDIGQGDSILVREWTGKNLLIDVGGRPFFSSKEHWRRGHHVANAQKTLIPYLKSRGIHTIDQLLVTHADT
DHMGDIEVVAKAIRIKEILTSQGSLSHPSFVRRLRRLKCHVRVLAAGDQLPIMGSVLQVLYPWQLGDGKNNDSLVLYGRL
LNRTFLFTGDLEKEGENEIIKRYPQLRVDYLKAGHHGSNTSSSAAFLDHIQPKVAFISAGKNNRYQHPHRETLARLEDRQ
ITYYRTDTQGAIRLTGRTSWHLETVR

Specific function: The comE operon is required for the binding and uptake of transforming DNA. ComEC is required for internalization but is dispensable for DNA binding [H]

COG id: COG0658

COG function: function code R; Predicted membrane metal-binding protein

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: To H.influenzae REC2, N.gonorrhoeae ComA and E.coli YcaI [H]

Homologues:

Organism=Escherichia coli, GI87081801, Length=638, Percent_Identity=23.0407523510972, Blast_Score=115, Evalue=1e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279
- InterPro:   IPR004477
- InterPro:   IPR004797 [H]

Pfam domain/function: PF03772 Competence; PF00753 Lactamase_B [H]

EC number: NA

Molecular weight: Translated: 85523; Mature: 85391

Theoretical pI: Translated: 9.71; Mature: 9.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFSWTKLVPLSKIQFAFLILVFFYQIHSPSWLTFLLSLSLICLLVKRLSKKEFLGVFAI
CCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSFCALFLLYQKQQLVQKLEIQPVQITSVALVPDSIRINGDQLAVLGRHGKHSYQLFYRL
HHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCEEECCCEEEEEECCCCHHHHHHHHH
KSQAEAQLFKKEHRWLVMHAKVTLEKAEEVRNFKGFNYQTFLTYQGIYRIGKVEQIEQLE
HHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHCCCCCEEEEEEEHHHHHHCCHHHHHHHH
VISPESICDYLSSLRRRAIVHCQQHFPRPMSHYLTGLLFGYLDKSFGEMTDYYSQLGIIH
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LFALSGMQVGFFLTCFRRVLLLLAVPLEWIKWIELPFACFYAALTGYSISVIRSLVQSQL
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
RHLGIKGLDNLACTFLLVFLWDAHFLMTVGGVLTFSYAFLLTVVTVEELSGAKRQLVQVL
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
TISLGILPFLLFYFSSFNPMSMVLTGLLSYLFDLFILPLLCLVFCLSPLVTVSICNHLFI
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLEKVIQFLGNTFNSSLVFGSPTSWHLLILVISFAIFYDYRQVRQRVITCGLVIALTLLS
HHHHHHHHHCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VKYPLTNEVTFIDIGQGDSILVREWTGKNLLIDVGGRPFFSSKEHWRRGHHVANAQKTLI
HCCCCCCCEEEEECCCCCEEEEEEECCCEEEEECCCCCCCCCHHHHHHCCHHCCHHHHHH
PYLKSRGIHTIDQLLVTHADTDHMGDIEVVAKAIRIKEILTSQGSLSHPSFVRRLRRLKC
HHHHCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
HVRVLAAGDQLPIMGSVLQVLYPWQLGDGKNNDSLVLYGRLLNRTFLFTGDLEKEGENEI
HEEEEECCCCCCHHHHHHHHHCCCCCCCCCCCCCEEEEEEHHCCEEEEECCCCCCCCHHH
IKRYPQLRVDYLKAGHHGSNTSSSAAFLDHIQPKVAFISAGKNNRYQHPHRETLARLEDR
HHHCHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHCCC
QITYYRTDTQGAIRLTGRTSWHLETVR
EEEEEEECCCCEEEEECCCCEEEEECC
>Mature Secondary Structure 
TFSWTKLVPLSKIQFAFLILVFFYQIHSPSWLTFLLSLSLICLLVKRLSKKEFLGVFAI
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSFCALFLLYQKQQLVQKLEIQPVQITSVALVPDSIRINGDQLAVLGRHGKHSYQLFYRL
HHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCEEECCCEEEEEECCCCHHHHHHHHH
KSQAEAQLFKKEHRWLVMHAKVTLEKAEEVRNFKGFNYQTFLTYQGIYRIGKVEQIEQLE
HHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHCCCCCEEEEEEEHHHHHHCCHHHHHHHH
VISPESICDYLSSLRRRAIVHCQQHFPRPMSHYLTGLLFGYLDKSFGEMTDYYSQLGIIH
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LFALSGMQVGFFLTCFRRVLLLLAVPLEWIKWIELPFACFYAALTGYSISVIRSLVQSQL
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
RHLGIKGLDNLACTFLLVFLWDAHFLMTVGGVLTFSYAFLLTVVTVEELSGAKRQLVQVL
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
TISLGILPFLLFYFSSFNPMSMVLTGLLSYLFDLFILPLLCLVFCLSPLVTVSICNHLFI
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLEKVIQFLGNTFNSSLVFGSPTSWHLLILVISFAIFYDYRQVRQRVITCGLVIALTLLS
HHHHHHHHHCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VKYPLTNEVTFIDIGQGDSILVREWTGKNLLIDVGGRPFFSSKEHWRRGHHVANAQKTLI
HCCCCCCCEEEEECCCCCEEEEEEECCCEEEEECCCCCCCCCHHHHHHCCHHCCHHHHHH
PYLKSRGIHTIDQLLVTHADTDHMGDIEVVAKAIRIKEILTSQGSLSHPSFVRRLRRLKC
HHHHCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
HVRVLAAGDQLPIMGSVLQVLYPWQLGDGKNNDSLVLYGRLLNRTFLFTGDLEKEGENEI
HEEEEECCCCCCHHHHHHHHHCCCCCCCCCCCCCEEEEEEHHCCEEEEECCCCCCCCHHH
IKRYPQLRVDYLKAGHHGSNTSSSAAFLDHIQPKVAFISAGKNNRYQHPHRETLARLEDR
HHHCHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHCCC
QITYYRTDTQGAIRLTGRTSWHLETVR
EEEEEEECCCCEEEEECCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7968523; 8969508; 9384377 [H]