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The map label for this gene is ytzG [H]

Identifier: 71910951

GI number: 71910951

Start: 1115975

End: 1116691

Strand: Reverse

Name: ytzG [H]

Synonym: M5005_Spy_1138

Alternate gene names: 71910951

Gene position: 1116691-1115975 (Counterclockwise)

Preceding gene: 71910952

Following gene: 71910950

Centisome position: 60.74

GC content: 41.84

Gene sequence:

>717_bases
ATGCGTTTAGATAAATTATTAGAAGGTACAAAAGTTGGCTCTAGAAGTCAAGTGAAGAAACTGATTAAGGCTCAAGGGGT
TTGGGTTGATCACATGCCTGCCAGAAATGGTCGACAAAATGTTGATCCTGGCTTGCAACTGATTGAGGTGACTGGACAGC
GAGTGACGCATCCTAAACATTCTTACATTATCCTCAACAAGCCATCAGGGGTGGTCTCAGCTAAGAAAGATACTAACTAT
TTAACCGTTATTGATCAGCTTGCTGAAGAAGATAAAAGTCCAGACTTGTATCCTGTTGGCAGATTGGATAGAGATACCGA
AGGGTTGGTTTTACTCACTGATAATGGTCCCCTAGGGTTTCGGATGTTGCACCCTAGTCATCATGTTTCTAAAACGTATC
TTGTAACCGTTAATGGCTTGTTAGCAGAAGATGCCTCAGATTTTTTTGCAGCTGGTATTTGTTTCCCAACAGGTGAACAA
TGCCAACCAGCTCAATTGACTATTCTTAAAGCCGATACAGACCAGTCACAGGCTAGTTTGACCATTTCAGAAGGAAAATT
TCACCAAGTGAAAAAAATGTTTTTGACCTATGGTTTGAAAGTGACCTCTCTGAAACGGACGCACTTTGCAGGACTGGAAC
TCGGTCATTTAGCCAGCGGAGAATATCGTTATCTAACAGCGTCAGAAAGGCAACTCATTAAAACGTATCTGGACTGA

Upstream 100 bases:

>100_bases
CTTGTAAGAACAAAAATGAGGAGATAGTCAACTTCATTTAAGAAGAAGTCTTGTAACTTCTATTTCTTTTGCAAGGGTAA
ATTTATGTTACACTACTAAT

Downstream 100 bases:

>100_bases
GCAAAACATGCTTTCAAAAAGAAGCTATTCTTGCTTAATCAATAAAGTGTCCATTGACTATTATAGTCATGATGAGTCCG
ACTTTTGCTATGTTTAGTCA

Product: ribosomal small subunit pseudouridine synthase A

Products: pseudouridine 5'-phosphate; H2O [C]

Alternate protein names: RNA pseudouridylate synthase; RNA-uridine isomerase [H]

Number of amino acids: Translated: 238; Mature: 238

Protein sequence:

>238_residues
MRLDKLLEGTKVGSRSQVKKLIKAQGVWVDHMPARNGRQNVDPGLQLIEVTGQRVTHPKHSYIILNKPSGVVSAKKDTNY
LTVIDQLAEEDKSPDLYPVGRLDRDTEGLVLLTDNGPLGFRMLHPSHHVSKTYLVTVNGLLAEDASDFFAAGICFPTGEQ
CQPAQLTILKADTDQSQASLTISEGKFHQVKKMFLTYGLKVTSLKRTHFAGLELGHLASGEYRYLTASERQLIKTYLD

Sequences:

>Translated_238_residues
MRLDKLLEGTKVGSRSQVKKLIKAQGVWVDHMPARNGRQNVDPGLQLIEVTGQRVTHPKHSYIILNKPSGVVSAKKDTNY
LTVIDQLAEEDKSPDLYPVGRLDRDTEGLVLLTDNGPLGFRMLHPSHHVSKTYLVTVNGLLAEDASDFFAAGICFPTGEQ
CQPAQLTILKADTDQSQASLTISEGKFHQVKKMFLTYGLKVTSLKRTHFAGLELGHLASGEYRYLTASERQLIKTYLD
>Mature_238_residues
MRLDKLLEGTKVGSRSQVKKLIKAQGVWVDHMPARNGRQNVDPGLQLIEVTGQRVTHPKHSYIILNKPSGVVSAKKDTNY
LTVIDQLAEEDKSPDLYPVGRLDRDTEGLVLLTDNGPLGFRMLHPSHHVSKTYLVTVNGLLAEDASDFFAAGICFPTGEQ
CQPAQLTILKADTDQSQASLTISEGKFHQVKKMFLTYGLKVTSLKRTHFAGLELGHLASGEYRYLTASERQLIKTYLD

Specific function: Responsible For Synthesis Of Pseudouridine From Uracil-516 In 16s Ribosomal RNA. [C]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788510, Length=238, Percent_Identity=37.3949579831933, Blast_Score=127, Evalue=6e-31,
Organism=Escherichia coli, GI1790453, Length=237, Percent_Identity=29.535864978903, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI1787524, Length=223, Percent_Identity=28.6995515695067, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI87081838, Length=200, Percent_Identity=28.5, Blast_Score=76, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: 4.2.1.70 [C]

Molecular weight: Translated: 26416; Mature: 26416

Theoretical pI: Translated: 8.98; Mature: 8.98

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLDKLLEGTKVGSRSQVKKLIKAQGVWVDHMPARNGRQNVDPGLQLIEVTGQRVTHPKH
CCHHHHHCCCCCCCHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCEEEEECCCCCCCCCC
SYIILNKPSGVVSAKKDTNYLTVIDQLAEEDKSPDLYPVGRLDRDTEGLVLLTDNGPLGF
CEEEEECCCCCEEECCCCCHHHHHHHHHHHCCCCCEEECCCCCCCCCCEEEEECCCCEEE
RMLHPSHHVSKTYLVTVNGLLAEDASDFFAAGICFPTGEQCQPAQLTILKADTDQSQASL
EEECCCCCCCEEEEEEECCEECCCCHHHHEEEEECCCCCCCCCCEEEEEECCCCCCCEEE
TISEGKFHQVKKMFLTYGLKVTSLKRTHFAGLELGHLASGEYRYLTASERQLIKTYLD
EECCCHHHHHHHHHHHHCCEEEEECCHHCCCCEECCCCCCCEEEEECHHHHHHHHHCC
>Mature Secondary Structure
MRLDKLLEGTKVGSRSQVKKLIKAQGVWVDHMPARNGRQNVDPGLQLIEVTGQRVTHPKH
CCHHHHHCCCCCCCHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCEEEEECCCCCCCCCC
SYIILNKPSGVVSAKKDTNYLTVIDQLAEEDKSPDLYPVGRLDRDTEGLVLLTDNGPLGF
CEEEEECCCCCEEECCCCCHHHHHHHHHHHCCCCCEEECCCCCCCCCCEEEEECCCCEEE
RMLHPSHHVSKTYLVTVNGLLAEDASDFFAAGICFPTGEQCQPAQLTILKADTDQSQASL
EEECCCCCCCEEEEEEECCEECCCCHHHHEEEEECCCCCCCCCCEEEEEECCCCCCCEEE
TISEGKFHQVKKMFLTYGLKVTSLKRTHFAGLELGHLASGEYRYLTASERQLIKTYLD
EECCCHHHHHHHHHHHHCCEEEEECCHHCCCCEECCCCCCCEEEEECHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose phosphate [C]

Specific reaction: uracil + D-ribose phosphate = pseudouridine 5'-phosphate + H2O [C]

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]