Click here to switch to the map view.

The map label for this gene is alaS [H]

Identifier: 71910945

GI number: 71910945

Start: 1106432

End: 1109050

Strand: Reverse

Name: alaS [H]

Synonym: M5005_Spy_1132

Alternate gene names: 71910945

Gene position: 1109050-1106432 (Counterclockwise)

Preceding gene: 71910946

Following gene: 71910944

Centisome position: 60.32

GC content: 42.65

Gene sequence:

>2619_bases
ATGAAAGAATTATCGTCTGCACAAATCCGCCAAATGTGGTTGGATTTCTGGAAATCTAAAGGACATTGCGTTGAGCCTTC
AGCTAACTTGGTTCCTGTGAACGACCCAACGCTTCTTTGGATCAACTCAGGTGTTGCAACCTTGAAAAAATATTTTGATG
GTTCAGTGATTCCAGAAAATCCACGTATTACCAATGCACAAAAATCAATTCGTACTAATGATATTGAAAATGTTGGTAAA
ACAGCACGTCACCATACTATGTTTGAAATGCTTGGTAACTTCTCAATTGGAGACTATTTCCGTGATGAAGCTATTGAGTG
GGGATTTGAACTCTTGACAAGTCCAGACTGGTTTGATTTCCCTAAAGACAAGCTCTACATGACTTATTACCCAGATGACA
AGGATTCGTATAACCGTTGGATTGCTTGTGGCGTTGAACCAAGTCACTTGGTGCCGATCGAGGATAACTTCTGGGAAATC
GGTGCTGGTCCTTCAGGTCCAGATACGGAGATTTTCTTCGACCGTGGTGAAGATTTCGATCCAGAAAATATCGGACTTCG
CCTCTTGGCTGAAGATATCGAAAACGATCGTTACATCGAAATCTGGAACATCGTTCTCTCACAATTCAATGCTGACCCAG
CCGTACCACGTTCAGAATACAAAGAATTACCAAACAAAAACATTGATACAGGTGCTGGTCTTGAACGTCTTGCAGCTGTT
ATGCAAGGGGCAAAAACAAACTTTGAAACTGACCTCTTCATGCCAATCATCCGTGAAGTAGAGAAGTTGTCAGGTAAAAC
TTACGATCCAGATGGCGACAACATGAGTTTCAAGGTTATCGCTGACCACATCCGTGCGCTTTCATTTGCTATCGGTGATG
GTGCGCTTCCTGGAAATGAAGGTCGTGGTTACGTTCTTCGTCGTCTTCTCCGTCGTGCGGTTATGCACGGTCGCCGTCTT
GGCATCAACGAAACTTTCCTTTACAAATTGGTTCCGACTGTTGGACAAATCATGGAAAGCTACTACCCAGAAGTGCTTGA
AAAACGTGATTTTATCGAGAAAATCGTTAAACGTGAGGAAGAAACATTTGCTCGTACTATCGATGCAGGTAGCGGTCACT
TAGATTCATTGCTTGCGCAGCTTAAGGCTGAAGGTAAGGATACTCTTGAAGGTAAAGATATCTTCAAACTTTATGATACT
TATGGATTCCCGGTTGAATTGACAGAGGAATTGGCAGAAGATGCAGGCTACAAGATTGACCACGAAGGCTTTAAGTCAGC
CATGAAAGAACAACAAGACCGTGCGCGTGCAGCTGTTGTTAAGGGTGGTTCAATGGGGATGCAAAATGAAACCCTAGCTG
GTATTGTTGAAGAATCACGATTCGAATACGACACATATAGTCTTGAATCAAGTCTTTCAGTCATCATCGCTGATAATGAA
CGTACCGAAGCTGTTTCAGAAGGTCAAGCCCTTCTTGTCTTTGCTCAAACACCATTCTATGCTGAAATGGGTGGACAGGT
TGCTGACACAGGTAGAATCAAAAATGATAAGGGTGACACAGTTGCTGAGGTTGTTGATGTTCAAAAAGCACCAAATGGTC
AACCTCTACACACTGTAAACGTTTTAGCATCACTTTCAGTTGGAACAAACTACACACTTGAAATCAACAAAGAGCGTCGT
TTGGCTGTTGAGAAAAACCACACAGCTACTCACTTGCTCCATGCAGCTCTTCACAATGTTATCGGTGAACACGCAACTCA
GGCTGGTTCATTGAACGAAGAAGAATTCTTGCGCTTTGATTTTACTCACTTTGAAGCAGTAAGCAATGAGGAACTTCGTC
ACATTGAACAAGAAGTTAATGAGCAAATTTGGAACGCTCTTACAATCACAACGACTGAAACTGACGTTGAAACCGCAAAA
GAGATGGGAGCAATGGCGCTTTTTGGTGAGAAATATGGTAAAGTGGTTCGTGTGGTTCAAATTGGTAATTATTCTGTTGA
ACTTTGTGGTGGAACTCACTTAAATAATTCTTCAGAAATCGGTCTCTTCAAGATTGTCAAAGAAGAAGGTATTGGTTCAG
GCACTCGTCGTATTATTGCAGTTACTGGTAGACAAGCTTTTGAAGCTTATCGTAACCAAGAGGATGCCCTAAAAGAGATC
GCTGCTACTGTAAAAGCTCCGCAATTGAAAGATGCAGCAGCTAAAGTACAAGCTCTTAGCGACTCGCTTCGTGATCTTCA
AAAAGAAAATGCAGAACTTAAAGAAAAAGCAGCAGCTGCAGCAGCTGGTGATGTCTTTAAAGATGTTCAAGAAGCTAAGG
GCGTGCGCTTCATTGCTAGTCAAGTTGATGTTGCAGATGCAGGGGCACTTCGTACATTTGCTGATAACTGGAAACAAAAA
GACTACTCTGATGTGCTTGTTCTCGTAGCAGCTATTGGTGAGAAGGTTAATGTCCTTGTTGCAAGCAAAACCAAAGATGT
CCACGCTGGTAACATGATCAAAGAATTGGCACCAATTGTAGCAGGTCGTGGTGGAGGTAAACCAGACATGGCTATGGCAG
GTGGTAGCGATGCAAGTAAAATTGCAGAGCTGCTAGCAGCAGTTGCTGAAATAGTGTAA

Upstream 100 bases:

>100_bases
CTGATCTCCTAGCAAGAAAGAAGAGCTGTGTCAACTGGTGTGATTACATTACTAGCTTCAGCATAACACTGTTTTTCTTA
CTCTTTGTATCTAAATTTTT

Downstream 100 bases:

>100_bases
ATCCATAAAAAACCAAGTTATTTAGAACTTGGTTTTTTATATTTGAAATGACTACCATCATAACCTTTTTGACTATTATC
GTTGTCAAAAAGAAAAAGAT

Product: alanyl-tRNA synthetase

Products: NA

Alternate protein names: Alanine--tRNA ligase; AlaRS [H]

Number of amino acids: Translated: 872; Mature: 872

Protein sequence:

>872_residues
MKELSSAQIRQMWLDFWKSKGHCVEPSANLVPVNDPTLLWINSGVATLKKYFDGSVIPENPRITNAQKSIRTNDIENVGK
TARHHTMFEMLGNFSIGDYFRDEAIEWGFELLTSPDWFDFPKDKLYMTYYPDDKDSYNRWIACGVEPSHLVPIEDNFWEI
GAGPSGPDTEIFFDRGEDFDPENIGLRLLAEDIENDRYIEIWNIVLSQFNADPAVPRSEYKELPNKNIDTGAGLERLAAV
MQGAKTNFETDLFMPIIREVEKLSGKTYDPDGDNMSFKVIADHIRALSFAIGDGALPGNEGRGYVLRRLLRRAVMHGRRL
GINETFLYKLVPTVGQIMESYYPEVLEKRDFIEKIVKREEETFARTIDAGSGHLDSLLAQLKAEGKDTLEGKDIFKLYDT
YGFPVELTEELAEDAGYKIDHEGFKSAMKEQQDRARAAVVKGGSMGMQNETLAGIVEESRFEYDTYSLESSLSVIIADNE
RTEAVSEGQALLVFAQTPFYAEMGGQVADTGRIKNDKGDTVAEVVDVQKAPNGQPLHTVNVLASLSVGTNYTLEINKERR
LAVEKNHTATHLLHAALHNVIGEHATQAGSLNEEEFLRFDFTHFEAVSNEELRHIEQEVNEQIWNALTITTTETDVETAK
EMGAMALFGEKYGKVVRVVQIGNYSVELCGGTHLNNSSEIGLFKIVKEEGIGSGTRRIIAVTGRQAFEAYRNQEDALKEI
AATVKAPQLKDAAAKVQALSDSLRDLQKENAELKEKAAAAAAGDVFKDVQEAKGVRFIASQVDVADAGALRTFADNWKQK
DYSDVLVLVAAIGEKVNVLVASKTKDVHAGNMIKELAPIVAGRGGGKPDMAMAGGSDASKIAELLAAVAEIV

Sequences:

>Translated_872_residues
MKELSSAQIRQMWLDFWKSKGHCVEPSANLVPVNDPTLLWINSGVATLKKYFDGSVIPENPRITNAQKSIRTNDIENVGK
TARHHTMFEMLGNFSIGDYFRDEAIEWGFELLTSPDWFDFPKDKLYMTYYPDDKDSYNRWIACGVEPSHLVPIEDNFWEI
GAGPSGPDTEIFFDRGEDFDPENIGLRLLAEDIENDRYIEIWNIVLSQFNADPAVPRSEYKELPNKNIDTGAGLERLAAV
MQGAKTNFETDLFMPIIREVEKLSGKTYDPDGDNMSFKVIADHIRALSFAIGDGALPGNEGRGYVLRRLLRRAVMHGRRL
GINETFLYKLVPTVGQIMESYYPEVLEKRDFIEKIVKREEETFARTIDAGSGHLDSLLAQLKAEGKDTLEGKDIFKLYDT
YGFPVELTEELAEDAGYKIDHEGFKSAMKEQQDRARAAVVKGGSMGMQNETLAGIVEESRFEYDTYSLESSLSVIIADNE
RTEAVSEGQALLVFAQTPFYAEMGGQVADTGRIKNDKGDTVAEVVDVQKAPNGQPLHTVNVLASLSVGTNYTLEINKERR
LAVEKNHTATHLLHAALHNVIGEHATQAGSLNEEEFLRFDFTHFEAVSNEELRHIEQEVNEQIWNALTITTTETDVETAK
EMGAMALFGEKYGKVVRVVQIGNYSVELCGGTHLNNSSEIGLFKIVKEEGIGSGTRRIIAVTGRQAFEAYRNQEDALKEI
AATVKAPQLKDAAAKVQALSDSLRDLQKENAELKEKAAAAAAGDVFKDVQEAKGVRFIASQVDVADAGALRTFADNWKQK
DYSDVLVLVAAIGEKVNVLVASKTKDVHAGNMIKELAPIVAGRGGGKPDMAMAGGSDASKIAELLAAVAEIV
>Mature_872_residues
MKELSSAQIRQMWLDFWKSKGHCVEPSANLVPVNDPTLLWINSGVATLKKYFDGSVIPENPRITNAQKSIRTNDIENVGK
TARHHTMFEMLGNFSIGDYFRDEAIEWGFELLTSPDWFDFPKDKLYMTYYPDDKDSYNRWIACGVEPSHLVPIEDNFWEI
GAGPSGPDTEIFFDRGEDFDPENIGLRLLAEDIENDRYIEIWNIVLSQFNADPAVPRSEYKELPNKNIDTGAGLERLAAV
MQGAKTNFETDLFMPIIREVEKLSGKTYDPDGDNMSFKVIADHIRALSFAIGDGALPGNEGRGYVLRRLLRRAVMHGRRL
GINETFLYKLVPTVGQIMESYYPEVLEKRDFIEKIVKREEETFARTIDAGSGHLDSLLAQLKAEGKDTLEGKDIFKLYDT
YGFPVELTEELAEDAGYKIDHEGFKSAMKEQQDRARAAVVKGGSMGMQNETLAGIVEESRFEYDTYSLESSLSVIIADNE
RTEAVSEGQALLVFAQTPFYAEMGGQVADTGRIKNDKGDTVAEVVDVQKAPNGQPLHTVNVLASLSVGTNYTLEINKERR
LAVEKNHTATHLLHAALHNVIGEHATQAGSLNEEEFLRFDFTHFEAVSNEELRHIEQEVNEQIWNALTITTTETDVETAK
EMGAMALFGEKYGKVVRVVQIGNYSVELCGGTHLNNSSEIGLFKIVKEEGIGSGTRRIIAVTGRQAFEAYRNQEDALKEI
AATVKAPQLKDAAAKVQALSDSLRDLQKENAELKEKAAAAAAGDVFKDVQEAKGVRFIASQVDVADAGALRTFADNWKQK
DYSDVLVLVAAIGEKVNVLVASKTKDVHAGNMIKELAPIVAGRGGGKPDMAMAGGSDASKIAELLAAVAEIV

Specific function: Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction:alanine is first activated by ATP to form Ala- AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editin

COG id: COG0013

COG function: function code J; Alanyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI109148542, Length=976, Percent_Identity=34.0163934426229, Blast_Score=453, Evalue=1e-127,
Organism=Homo sapiens, GI38569417, Length=779, Percent_Identity=31.0654685494223, Blast_Score=335, Evalue=8e-92,
Organism=Escherichia coli, GI1789048, Length=884, Percent_Identity=43.552036199095, Blast_Score=674, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17506981, Length=972, Percent_Identity=30.9670781893004, Blast_Score=408, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI17536681, Length=761, Percent_Identity=32.7201051248357, Blast_Score=347, Evalue=2e-95,
Organism=Saccharomyces cerevisiae, GI6324911, Length=811, Percent_Identity=37.6078914919852, Blast_Score=486, Evalue=1e-138,
Organism=Drosophila melanogaster, GI24582809, Length=972, Percent_Identity=32.5102880658436, Blast_Score=451, Evalue=1e-126,
Organism=Drosophila melanogaster, GI45552267, Length=972, Percent_Identity=32.5102880658436, Blast_Score=451, Evalue=1e-126,
Organism=Drosophila melanogaster, GI24658214, Length=814, Percent_Identity=28.3783783783784, Blast_Score=277, Evalue=3e-74,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002318
- InterPro:   IPR018162
- InterPro:   IPR018165
- InterPro:   IPR018164
- InterPro:   IPR023033
- InterPro:   IPR016040
- InterPro:   IPR003156
- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF02272 DHHA1; PF01411 tRNA-synt_2c; PF07973 tRNA_SAD [H]

EC number: =6.1.1.7 [H]

Molecular weight: Translated: 96532; Mature: 96532

Theoretical pI: Translated: 4.66; Mature: 4.66

Prosite motif: PS50860 AA_TRNA_LIGASE_II_ALA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKELSSAQIRQMWLDFWKSKGHCVEPSANLVPVNDPTLLWINSGVATLKKYFDGSVIPEN
CCCCHHHHHHHHHHHHHCCCCCEECCCCCEEECCCCEEEEECCCHHHHHHHHCCCCCCCC
PRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFRDEAIEWGFELLTSPDWFDF
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCC
PKDKLYMTYYPDDKDSYNRWIACGVEPSHLVPIEDNFWEIGAGPSGPDTEIFFDRGEDFD
CCCCEEEEECCCCCCCCCCEEEECCCCCEEEECCCCCEEECCCCCCCCCCEEEECCCCCC
PENIGLRLLAEDIENDRYIEIWNIVLSQFNADPAVPRSEYKELPNKNIDTGAGLERLAAV
CCCCCHHEEEHHCCCCCEEHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCCCCCHHHHHHH
MQGAKTNFETDLFMPIIREVEKLSGKTYDPDGDNMSFKVIADHIRALSFAIGDGALPGNE
HHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCC
GRGYVLRRLLRRAVMHGRRLGINETFLYKLVPTVGQIMESYYPEVLEKRDFIEKIVKREE
CCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ETFARTIDAGSGHLDSLLAQLKAEGKDTLEGKDIFKLYDTYGFPVELTEELAEDAGYKID
HHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHCCCEEC
HEGFKSAMKEQQDRARAAVVKGGSMGMQNETLAGIVEESRFEYDTYSLESSLSVIIADNE
HHHHHHHHHHHHHHHHHHHEECCCCCCCCCHHHHHHHHHCCCCCCEECCCCCEEEEECCC
RTEAVSEGQALLVFAQTPFYAEMGGQVADTGRIKNDKGDTVAEVVDVQKAPNGQPLHTVN
HHHHHCCCCEEEEEECCCCHHHHCCCEECCCCCCCCCCCHHHHHHHHHCCCCCCCCHHHH
VLASLSVGTNYTLEINKERRLAVEKNHTATHLLHAALHNVIGEHATQAGSLNEEEFLRFD
HHHHHCCCCCEEEEECCCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHEEC
FTHFEAVSNEELRHIEQEVNEQIWNALTITTTETDVETAKEMGAMALFGEKYGKVVRVVQ
HHHHHHCCHHHHHHHHHHHHHHHHCEEEEEECCCHHHHHHHHHHHHHHHHHHCCEEEEEE
IGNYSVELCGGTHLNNSSEIGLFKIVKEEGIGSGTRRIIAVTGRQAFEAYRNQEDALKEI
ECCEEEEEECCCCCCCCCCCCCEEEEHHCCCCCCCCEEEEECCHHHHHHHCCHHHHHHHH
AATVKAPQLKDAAAKVQALSDSLRDLQKENAELKEKAAAAAAGDVFKDVQEAKGVRFIAS
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEHHH
QVDVADAGALRTFADNWKQKDYSDVLVLVAAIGEKVNVLVASKTKDVHAGNMIKELAPIV
HCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHH
AGRGGGKPDMAMAGGSDASKIAELLAAVAEIV
HCCCCCCCCCEECCCCCHHHHHHHHHHHHHHC
>Mature Secondary Structure
MKELSSAQIRQMWLDFWKSKGHCVEPSANLVPVNDPTLLWINSGVATLKKYFDGSVIPEN
CCCCHHHHHHHHHHHHHCCCCCEECCCCCEEECCCCEEEEECCCHHHHHHHHCCCCCCCC
PRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFRDEAIEWGFELLTSPDWFDF
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCC
PKDKLYMTYYPDDKDSYNRWIACGVEPSHLVPIEDNFWEIGAGPSGPDTEIFFDRGEDFD
CCCCEEEEECCCCCCCCCCEEEECCCCCEEEECCCCCEEECCCCCCCCCCEEEECCCCCC
PENIGLRLLAEDIENDRYIEIWNIVLSQFNADPAVPRSEYKELPNKNIDTGAGLERLAAV
CCCCCHHEEEHHCCCCCEEHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCCCCCHHHHHHH
MQGAKTNFETDLFMPIIREVEKLSGKTYDPDGDNMSFKVIADHIRALSFAIGDGALPGNE
HHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCC
GRGYVLRRLLRRAVMHGRRLGINETFLYKLVPTVGQIMESYYPEVLEKRDFIEKIVKREE
CCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ETFARTIDAGSGHLDSLLAQLKAEGKDTLEGKDIFKLYDTYGFPVELTEELAEDAGYKID
HHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHCCCEEC
HEGFKSAMKEQQDRARAAVVKGGSMGMQNETLAGIVEESRFEYDTYSLESSLSVIIADNE
HHHHHHHHHHHHHHHHHHHEECCCCCCCCCHHHHHHHHHCCCCCCEECCCCCEEEEECCC
RTEAVSEGQALLVFAQTPFYAEMGGQVADTGRIKNDKGDTVAEVVDVQKAPNGQPLHTVN
HHHHHCCCCEEEEEECCCCHHHHCCCEECCCCCCCCCCCHHHHHHHHHCCCCCCCCHHHH
VLASLSVGTNYTLEINKERRLAVEKNHTATHLLHAALHNVIGEHATQAGSLNEEEFLRFD
HHHHHCCCCCEEEEECCCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHEEC
FTHFEAVSNEELRHIEQEVNEQIWNALTITTTETDVETAKEMGAMALFGEKYGKVVRVVQ
HHHHHHCCHHHHHHHHHHHHHHHHCEEEEEECCCHHHHHHHHHHHHHHHHHHCCEEEEEE
IGNYSVELCGGTHLNNSSEIGLFKIVKEEGIGSGTRRIIAVTGRQAFEAYRNQEDALKEI
ECCEEEEEECCCCCCCCCCCCCEEEEHHCCCCCCCCEEEEECCHHHHHHHCCHHHHHHHH
AATVKAPQLKDAAAKVQALSDSLRDLQKENAELKEKAAAAAAGDVFKDVQEAKGVRFIAS
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEHHH
QVDVADAGALRTFADNWKQKDYSDVLVLVAAIGEKVNVLVASKTKDVHAGNMIKELAPIV
HCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHH
AGRGGGKPDMAMAGGSDASKIAELLAAVAEIV
HCCCCCCCCCEECCCCCHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA