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The map label for this gene is ygcA [C]

Identifier: 71910911

GI number: 71910911

Start: 1070348

End: 1071703

Strand: Reverse

Name: ygcA [C]

Synonym: M5005_Spy_1098

Alternate gene names: 71910911

Gene position: 1071703-1070348 (Counterclockwise)

Preceding gene: 71910912

Following gene: 71910910

Centisome position: 58.29

GC content: 41.08

Gene sequence:

>1356_bases
ATGTTGAAAAAAAATGATATTATCCAAGTTGCTATTTCAGATTTAAGCCATGAGGGAGCAGGTGTCGCTAAACACGATGG
TTTTGTTTTCTTTGTTGATAATGCCTTGCCAGAAGAAGTGATTGACATGCGTGTCTTAAAGGTCAATAAAAACAGCGGTT
TTGGCAAAGTAGAAGCCTATCATTACCTGTCGTCAGCTAGAAATGCAGATGTCAATTTAACTTATTTGCGAACAGGCATT
GCAGACCTTGGACATCTAACTTATGAAGACCAACTGACTTTCAAAAAGAAACAAGTTCAGGACAGCCTCTATAAAATTGC
TGGAATCAGTGACGTGACAGTCGAAAGCACGATTGGGATGACAGAACCTTTAGCTTACCGAAATAAGGCTCAAGTTCCTG
TTAGACGTGTTAATGGTCAGCTAGAAACAGGCTTTTTCCGCAAGCATTCTCATGACTTGATTCCTATTTCGGATTATTAT
ATTCAGGACAAAGAGATTGATCGTCTCATTAATTTTACCCGTGATTTATTACGCCGCTTTGATATTAAACCGTATGACGA
AACAGAACAAACAGGTCTCTTGAGAAATATTGTTGTTCGCCGAGGTCATTACTCTGGAGAAATGATGCTGGTCTTAGTGA
CTACACGCCCAAAAGTTTTCCGTGTTGACCAAGTCATTGAAAAAATCGTTGAAGCTTTTCCAGCAGTGGTGTCTATTATT
CAAAATATTAATGATAAGAACACCAATGCAATCTTTGGTAAAGATTTCAAAACGCTTTACGGCAAGGATACCATTACTGA
TAGCATGCTTGGAAACAACTATGCCATTTCTGCTCAGTCTTTCTACCAAGTGAATACGGTTATGGCAGAAAAACTCTATC
AAACAGCTATAGCCTTTTCAGACCTTTCTAAAGATGATATTGTGATTGACGCCTACTCAGGTATCGGAACCATTGGCCTA
TCATTTGCTAAAACCGTTAAAGCTGTTTATGGTGTTGAAGTGATTGAAGCAGCTGTTAGAGATGCTCAACAGAATGCCGC
TCTTAATGGCATCACCAATGCCTACTTTGTCGCAGATACCGCAGAACATGCCATGGCAACATGGGCGAAAGATGGCATCA
AACCAAGTGTTATTCTGGTAGACCCGCCACGAAAAGGCTTAACAGAAAGTTTCATCCAAGCCAGCGTGGCAATGGGACCA
CAAAAAATTACCTACGTTTCCTGCAACCCAGCCACCATGGCGCGTGACATAAAGCGTTATCAAGAGTTGGGTTACAAACT
GGCCAAAGTACAGCCCGTAGATTTGTTTCCGCAAACGCATCACGTCGAGTGCGTAGTGTTGCTAATCAAGGAGTAA

Upstream 100 bases:

>100_bases
CAAATTAGGTCTGCGCTAAGATATGGTATAATGGAGAGTAACTAAAGTTGCTCTTCTTTTTTGAGCAATGGTTAGCCCTA
GAAAAGGAAAAAGAATAAGA

Downstream 100 bases:

>100_bases
GGTAGCTGCCTCCACATGACAAAAATAAGGAGAAACGAATGCCTATTACCAAACACGAGATTCCCCTTTTAGAATGTGAT
AGTAATCCATCAGCCGTCAT

Product: tRNA (uracil-5-)-methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 451; Mature: 451

Protein sequence:

>451_residues
MLKKNDIIQVAISDLSHEGAGVAKHDGFVFFVDNALPEEVIDMRVLKVNKNSGFGKVEAYHYLSSARNADVNLTYLRTGI
ADLGHLTYEDQLTFKKKQVQDSLYKIAGISDVTVESTIGMTEPLAYRNKAQVPVRRVNGQLETGFFRKHSHDLIPISDYY
IQDKEIDRLINFTRDLLRRFDIKPYDETEQTGLLRNIVVRRGHYSGEMMLVLVTTRPKVFRVDQVIEKIVEAFPAVVSII
QNINDKNTNAIFGKDFKTLYGKDTITDSMLGNNYAISAQSFYQVNTVMAEKLYQTAIAFSDLSKDDIVIDAYSGIGTIGL
SFAKTVKAVYGVEVIEAAVRDAQQNAALNGITNAYFVADTAEHAMATWAKDGIKPSVILVDPPRKGLTESFIQASVAMGP
QKITYVSCNPATMARDIKRYQELGYKLAKVQPVDLFPQTHHVECVVLLIKE

Sequences:

>Translated_451_residues
MLKKNDIIQVAISDLSHEGAGVAKHDGFVFFVDNALPEEVIDMRVLKVNKNSGFGKVEAYHYLSSARNADVNLTYLRTGI
ADLGHLTYEDQLTFKKKQVQDSLYKIAGISDVTVESTIGMTEPLAYRNKAQVPVRRVNGQLETGFFRKHSHDLIPISDYY
IQDKEIDRLINFTRDLLRRFDIKPYDETEQTGLLRNIVVRRGHYSGEMMLVLVTTRPKVFRVDQVIEKIVEAFPAVVSII
QNINDKNTNAIFGKDFKTLYGKDTITDSMLGNNYAISAQSFYQVNTVMAEKLYQTAIAFSDLSKDDIVIDAYSGIGTIGL
SFAKTVKAVYGVEVIEAAVRDAQQNAALNGITNAYFVADTAEHAMATWAKDGIKPSVILVDPPRKGLTESFIQASVAMGP
QKITYVSCNPATMARDIKRYQELGYKLAKVQPVDLFPQTHHVECVVLLIKE
>Mature_451_residues
MLKKNDIIQVAISDLSHEGAGVAKHDGFVFFVDNALPEEVIDMRVLKVNKNSGFGKVEAYHYLSSARNADVNLTYLRTGI
ADLGHLTYEDQLTFKKKQVQDSLYKIAGISDVTVESTIGMTEPLAYRNKAQVPVRRVNGQLETGFFRKHSHDLIPISDYY
IQDKEIDRLINFTRDLLRRFDIKPYDETEQTGLLRNIVVRRGHYSGEMMLVLVTTRPKVFRVDQVIEKIVEAFPAVVSII
QNINDKNTNAIFGKDFKTLYGKDTITDSMLGNNYAISAQSFYQVNTVMAEKLYQTAIAFSDLSKDDIVIDAYSGIGTIGL
SFAKTVKAVYGVEVIEAAVRDAQQNAALNGITNAYFVADTAEHAMATWAKDGIKPSVILVDPPRKGLTESFIQASVAMGP
QKITYVSCNPATMARDIKRYQELGYKLAKVQPVDLFPQTHHVECVVLLIKE

Specific function: Could Be A 23s rRNA (Uracil-5-)-Methyltransferase. [C]

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI269784680, Length=428, Percent_Identity=27.1028037383178, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI269784676, Length=428, Percent_Identity=27.1028037383178, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI20149688, Length=428, Percent_Identity=27.1028037383178, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI34222389, Length=296, Percent_Identity=29.7297297297297, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI51173878, Length=296, Percent_Identity=29.7297297297297, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI269784678, Length=365, Percent_Identity=27.6712328767123, Blast_Score=111, Evalue=1e-24,
Organism=Escherichia coli, GI1789148, Length=457, Percent_Identity=26.9146608315098, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI1787083, Length=331, Percent_Identity=27.7945619335347, Blast_Score=127, Evalue=2e-30,
Organism=Escherichia coli, GI1790403, Length=186, Percent_Identity=32.258064516129, Blast_Score=89, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI71997438, Length=488, Percent_Identity=24.3852459016393, Blast_Score=106, Evalue=3e-23,
Organism=Saccharomyces cerevisiae, GI6322909, Length=443, Percent_Identity=24.1534988713318, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24666579, Length=361, Percent_Identity=29.3628808864266, Blast_Score=136, Evalue=2e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016027
- InterPro:   IPR001566
- InterPro:   IPR002792
- InterPro:   IPR010280 [H]

Pfam domain/function: PF01938 TRAM; PF05958 tRNA_U5-meth_tr [H]

EC number: NA

Molecular weight: Translated: 50423; Mature: 50423

Theoretical pI: Translated: 7.08; Mature: 7.08

Prosite motif: PS50926 TRAM ; PS01231 TRMA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKKNDIIQVAISDLSHEGAGVAKHDGFVFFVDNALPEEVIDMRVLKVNKNSGFGKVEAY
CCCCCCEEEEEEHHCCCCCCCEEECCCEEEEECCCCCHHHHCEEEEEEECCCCCCCHHHH
HYLSSARNADVNLTYLRTGIADLGHLTYEDQLTFKKKQVQDSLYKIAGISDVTVESTIGM
HHHHHCCCCCEEEEEEHHHHHHHCCCEECHHHHHHHHHHHHHHHHHCCCCCCEEHHHCCC
TEPLAYRNKAQVPVRRVNGQLETGFFRKHSHDLIPISDYYIQDKEIDRLINFTRDLLRRF
CCCHHCCCCCCCCHHHHCCCCCCCHHHHCCCCEEECHHCEECHHHHHHHHHHHHHHHHHC
DIKPYDETEQTGLLRNIVVRRGHYSGEMMLVLVTTRPKVFRVDQVIEKIVEAFPAVVSII
CCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEECCCCEEEHHHHHHHHHHHHHHHHHHH
QNINDKNTNAIFGKDFKTLYGKDTITDSMLGNNYAISAQSFYQVNTVMAEKLYQTAIAFS
HHCCCCCCCEEECCCHHHHCCCCHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHH
DLSKDDIVIDAYSGIGTIGLSFAKTVKAVYGVEVIEAAVRDAQQNAALNGITNAYFVADT
CCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECC
AEHAMATWAKDGIKPSVILVDPPRKGLTESFIQASVAMGPQKITYVSCNPATMARDIKRY
HHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHH
QELGYKLAKVQPVDLFPQTHHVECVVLLIKE
HHHCCEEHEECCCCCCCCCCCCEEEEEEEEC
>Mature Secondary Structure
MLKKNDIIQVAISDLSHEGAGVAKHDGFVFFVDNALPEEVIDMRVLKVNKNSGFGKVEAY
CCCCCCEEEEEEHHCCCCCCCEEECCCEEEEECCCCCHHHHCEEEEEEECCCCCCCHHHH
HYLSSARNADVNLTYLRTGIADLGHLTYEDQLTFKKKQVQDSLYKIAGISDVTVESTIGM
HHHHHCCCCCEEEEEEHHHHHHHCCCEECHHHHHHHHHHHHHHHHHCCCCCCEEHHHCCC
TEPLAYRNKAQVPVRRVNGQLETGFFRKHSHDLIPISDYYIQDKEIDRLINFTRDLLRRF
CCCHHCCCCCCCCHHHHCCCCCCCHHHHCCCCEEECHHCEECHHHHHHHHHHHHHHHHHC
DIKPYDETEQTGLLRNIVVRRGHYSGEMMLVLVTTRPKVFRVDQVIEKIVEAFPAVVSII
CCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEECCCCEEEHHHHHHHHHHHHHHHHHHH
QNINDKNTNAIFGKDFKTLYGKDTITDSMLGNNYAISAQSFYQVNTVMAEKLYQTAIAFS
HHCCCCCCCEEECCCHHHHCCCCHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHH
DLSKDDIVIDAYSGIGTIGLSFAKTVKAVYGVEVIEAAVRDAQQNAALNGITNAYFVADT
CCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECC
AEHAMATWAKDGIKPSVILVDPPRKGLTESFIQASVAMGPQKITYVSCNPATMARDIKRY
HHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHH
QELGYKLAKVQPVDLFPQTHHVECVVLLIKE
HHHCCEEHEECCCCCCCCCCCCEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12122206; 12799345 [H]