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The map label for this gene is malE [H]

Identifier: 71910871

GI number: 71910871

Start: 1027874

End: 1029325

Strand: Direct

Name: malE [H]

Synonym: M5005_Spy_1058

Alternate gene names: 71910871

Gene position: 1027874-1029325 (Clockwise)

Preceding gene: 71910867

Following gene: 71910872

Centisome position: 55.91

GC content: 37.26

Gene sequence:

>1452_bases
ATGGGTTTGTCATGTATTTCGTTTCCATTATACAATTATCTTTCAAAAAATCAACTTCAATCAAGATTCCCTTGTCATTT
TAACTCAAATTCTCTAAACTATTTTTTTAAAAAAAACGACCTTTTTTTTAAAAAAAGGCTTGACTTTTTCTGCAAGCGGT
TGCATAATATTAAGTGTAAGAAATATATCTTAGGAGGAAATAGCTATGAAATCATGGCAAAAAGTTATCGTCGGCGGAGC
AAGTTTGACACTTGCAAGTACTTGTTAGTGGGATGTGGATCAGGTTCAAAAGATAAAAAGGAAGCTGGCGCTGACTCAAA
GACTATTAAACTTTGGGTACCGACTGGCTCTAAAAAATCATATGCAGATACCATTGCAAAATTTGAAAAAGATTCTGGTT
ACACCGTTAAAGTGGTTGAATCAGAAGATCCAAAAGCACAAGAAAAAATCAAAAAAGATGCCTCAACTGCTGCCGATGTC
TTTTCACTTCCTCATGATCAACTTGGTCAACTTGTAGAATCTGGTACTATTCAAGAAGTTCCAGAAAAGTACAATAAAGA
AATTGCTGCTACTTCTACTGATCAGGCGCTCGTCGGTGCACAGTACAAAGGGAAAACATATGCTTTCCCATTTGGTATTG
AATCACAAGTTCTTTTCTACAATAAATCAAAATTAGCTGCTGAAGACGTTACTTCATATGATACAATTACTACAAAAGCA
ACTTTTGGTGGTACCTTTAAACAAGCTAACACTTACGCTACTGGTCCATTATTCATGTCAGTTGGTAACACTTTATTTGG
GGAAAACGGTGAAGATGTAAAAGGTACTAACTGGGGCAATGAAAAAGGCGCTGCTGTTCTTAAATGGATCGCAGACCAAG
CTTCAAATAAAGGTTTTGTTAGCTTAGATGCTAACAACGTTATGTCTAAATTTGGTGACGGTTCAGTAGCTTCCTTTGAA
TCAGGACCGTGGGACTATGAAGCTGCTCAAAAAGCTATCGGTAAAGAAAACCTTGGTGTTGCTATTTATCCAAAAGTAAC
TATTGGAGGCGAAACGGTTCAACAAAAAGCATTCTTGGGTGTAAAACTTTACGCAGTCAACCAAGCACCAGCTAAAGGTG
ATACAAAACGTATCGCAGCTAGCTACAAATTGGCATCCTACTTGACTAATGCTGAAAGCCAAGAAAACCAATTCAAAACT
CGTAACATTGTCCCAGCCAACAAAGAAGTTCAATCTTCAGAAGCTGTTCAATCAAACGAACTTGCTAAAACCGTTATCAC
TATGGGTTCTTCTTCAGACTACACTGTCGTAATGCCAAAACTTAGCCAAATGGGCACATTCTGGACTGAAAGTGCTGCTA
TTCTTAGTGATGCCTTCAACGGTAAAATCAAAGAAAACGACTACCTTACTAAGTTGCAACAATTCGACAAAGATATCGCT
GCAACAAAATAA

Upstream 100 bases:

>100_bases
CCTACTCACCGTCGAAGGGTTAACACCTGCCTTTTGAGCGACATCTTTTATTGTTACCATATTAACCTCGTTACTATATT
TACCAAAGATAAACCTTATC

Downstream 100 bases:

>100_bases
TATGATCTCATGTCATCACTGCTATACTAAAACAAGCACAAGGTAAGTACTTCTAAAAAGTTCTTTCCACTTACCTTATG
AAGAGTGGAACTAGGCTCCA

Product: maltose/maltodextrin-binding protein

Products: ADP; phosphate; maltose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 483; Mature: 482

Protein sequence:

>483_residues
MGLSCISFPLYNYLSKNQLQSRFPCHFNSNSLNYFFKKNDLFFKKRLDFFCKRLHNIKCKKYILGGNSYEIMAKSYRRRS
KFDTCKYLLVGCGSGSKDKKEAGADSKTIKLWVPTGSKKSYADTIAKFEKDSGYTVKVVESEDPKAQEKIKKDASTAADV
FSLPHDQLGQLVESGTIQEVPEKYNKEIAATSTDQALVGAQYKGKTYAFPFGIESQVLFYNKSKLAAEDVTSYDTITTKA
TFGGTFKQANTYATGPLFMSVGNTLFGENGEDVKGTNWGNEKGAAVLKWIADQASNKGFVSLDANNVMSKFGDGSVASFE
SGPWDYEAAQKAIGKENLGVAIYPKVTIGGETVQQKAFLGVKLYAVNQAPAKGDTKRIAASYKLASYLTNAESQENQFKT
RNIVPANKEVQSSEAVQSNELAKTVITMGSSSDYTVVMPKLSQMGTFWTESAAILSDAFNGKIKENDYLTKLQQFDKDIA
ATK

Sequences:

>Translated_483_residues
MGLSCISFPLYNYLSKNQLQSRFPCHFNSNSLNYFFKKNDLFFKKRLDFFCKRLHNIKCKKYILGGNSYEIMAKSYRRRS
KFDTCKYLLVGCGSGSKDKKEAGADSKTIKLWVPTGSKKSYADTIAKFEKDSGYTVKVVESEDPKAQEKIKKDASTAADV
FSLPHDQLGQLVESGTIQEVPEKYNKEIAATSTDQALVGAQYKGKTYAFPFGIESQVLFYNKSKLAAEDVTSYDTITTKA
TFGGTFKQANTYATGPLFMSVGNTLFGENGEDVKGTNWGNEKGAAVLKWIADQASNKGFVSLDANNVMSKFGDGSVASFE
SGPWDYEAAQKAIGKENLGVAIYPKVTIGGETVQQKAFLGVKLYAVNQAPAKGDTKRIAASYKLASYLTNAESQENQFKT
RNIVPANKEVQSSEAVQSNELAKTVITMGSSSDYTVVMPKLSQMGTFWTESAAILSDAFNGKIKENDYLTKLQQFDKDIA
ATK
>Mature_482_residues
GLSCISFPLYNYLSKNQLQSRFPCHFNSNSLNYFFKKNDLFFKKRLDFFCKRLHNIKCKKYILGGNSYEIMAKSYRRRSK
FDTCKYLLVGCGSGSKDKKEAGADSKTIKLWVPTGSKKSYADTIAKFEKDSGYTVKVVESEDPKAQEKIKKDASTAADVF
SLPHDQLGQLVESGTIQEVPEKYNKEIAATSTDQALVGAQYKGKTYAFPFGIESQVLFYNKSKLAAEDVTSYDTITTKAT
FGGTFKQANTYATGPLFMSVGNTLFGENGEDVKGTNWGNEKGAAVLKWIADQASNKGFVSLDANNVMSKFGDGSVASFES
GPWDYEAAQKAIGKENLGVAIYPKVTIGGETVQQKAFLGVKLYAVNQAPAKGDTKRIAASYKLASYLTNAESQENQFKTR
NIVPANKEVQSSEAVQSNELAKTVITMGSSSDYTVVMPKLSQMGTFWTESAAILSDAFNGKIKENDYLTKLQQFDKDIAA
TK

Specific function: Binding protein for cyclodextrin; involved in its cellular uptake. Interacts with all natural cyclodextrins:alpha, beta and gamma [H]

COG id: COG2182

COG function: function code G; Maltose-binding periplasmic proteins/domains

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 1 family [H]

Homologues:

Organism=Escherichia coli, GI1790466, Length=348, Percent_Identity=27.8735632183908, Blast_Score=82, Evalue=8e-17,

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 720 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 240 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006060
- InterPro:   IPR006059 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 53203; Mature: 53072

Theoretical pI: Translated: 9.47; Mature: 9.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLSCISFPLYNYLSKNQLQSRFPCHFNSNSLNYFFKKNDLFFKKRLDFFCKRLHNIKCK
CCCCHHHHHHHHHHHHHHHHHCCCEEECCCCEEEEEECCCHHHHHHHHHHHHHHCCCCEE
KYILGGNSYEIMAKSYRRRSKFDTCKYLLVGCGSGSKDKKEAGADSKTIKLWVPTGSKKS
EEEECCCCHHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHCCCCCCEEEEEECCCCCHH
YADTIAKFEKDSGYTVKVVESEDPKAQEKIKKDASTAADVFSLPHDQLGQLVESGTIQEV
HHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHH
PEKYNKEIAATSTDQALVGAQYKGKTYAFPFGIESQVLFYNKSKLAAEDVTSYDTITTKA
HHHHCCCEECCCCCCHHCCCCCCCCEEEECCCCCCEEEEEECCHHHHHHCCCCCCEEEEE
TFGGTFKQANTYATGPLFMSVGNTLFGENGEDVKGTNWGNEKGAAVLKWIADQASNKGFV
ECCCCHHCCCCEECCCCCEECCCHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEE
SLDANNVMSKFGDGSVASFESGPWDYEAAQKAIGKENLGVAIYPKVTIGGETVQQKAFLG
EECHHHHHHHCCCCCEECCCCCCCCHHHHHHHCCCCCCCEEEEEEEEECCHHHHHHHEEE
VKLYAVNQAPAKGDTKRIAASYKLASYLTNAESQENQFKTRNIVPANKEVQSSEAVQSNE
EEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHH
LAKTVITMGSSSDYTVVMPKLSQMGTFWTESAAILSDAFNGKIKENDYLTKLQQFDKDIA
HHHHHHEECCCCCEEEECCCHHHCCCHHCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
ATK
CCC
>Mature Secondary Structure 
GLSCISFPLYNYLSKNQLQSRFPCHFNSNSLNYFFKKNDLFFKKRLDFFCKRLHNIKCK
CCCHHHHHHHHHHHHHHHHHCCCEEECCCCEEEEEECCCHHHHHHHHHHHHHHCCCCEE
KYILGGNSYEIMAKSYRRRSKFDTCKYLLVGCGSGSKDKKEAGADSKTIKLWVPTGSKKS
EEEECCCCHHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHCCCCCCEEEEEECCCCCHH
YADTIAKFEKDSGYTVKVVESEDPKAQEKIKKDASTAADVFSLPHDQLGQLVESGTIQEV
HHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHH
PEKYNKEIAATSTDQALVGAQYKGKTYAFPFGIESQVLFYNKSKLAAEDVTSYDTITTKA
HHHHCCCEECCCCCCHHCCCCCCCCEEEECCCCCCEEEEEECCHHHHHHCCCCCCEEEEE
TFGGTFKQANTYATGPLFMSVGNTLFGENGEDVKGTNWGNEKGAAVLKWIADQASNKGFV
ECCCCHHCCCCEECCCCCEECCCHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEE
SLDANNVMSKFGDGSVASFESGPWDYEAAQKAIGKENLGVAIYPKVTIGGETVQQKAFLG
EECHHHHHHHCCCCCEECCCCCCCCHHHHHHHCCCCCCCEEEEEEEEECCHHHHHHHEEE
VKLYAVNQAPAKGDTKRIAASYKLASYLTNAESQENQFKTRNIVPANKEVQSSEAVQSNE
EEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHH
LAKTVITMGSSSDYTVVMPKLSQMGTFWTESAAILSDAFNGKIKENDYLTKLQQFDKDIA
HHHHHHEECCCCCEEEECCCHHHCCCHHCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
ATK
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; maltose [Periplasm]; H2O [C]

Specific reaction: ATP + maltose [Periplasm] + H2O = ADP + phosphate + maltose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]