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The map label for this gene is oadA1 [H]

Identifier: 71910722

GI number: 71910722

Start: 895298

End: 896692

Strand: Direct

Name: oadA1 [H]

Synonym: M5005_Spy_0909

Alternate gene names: 71910722

Gene position: 895298-896692 (Clockwise)

Preceding gene: 71910721

Following gene: 71910725

Centisome position: 48.7

GC content: 42.65

Gene sequence:

>1395_bases
ATGACGAAAATCCGTATAACGGAAACAGTTTTACGTGATGGTCAACAAAGTCAGATTGCCACACGTATGACAACCAAGGA
AATGATTCCTATTCTCGAAACGCTAGACAATGCTGGTTATCATGCCCTTGAAATGTGGGGAGGAGCAACCTTTGATTCCT
GCCTACGCTTTTTAAATGAAGACCCTTGGGAACGGCTAAGAGCCATCCGTAAAGCAGTGAAAAAGACTAAACTTCAGATG
CTCTTACGCGGACAAAACCTTCTTGGATATCGCAATTATGCTGACGATGTGGTCAGATCCTTTATTCAAAAATCCATTGA
AAATGGGATTGATATTGTCCGTATTTTCGATGCTTTAAACGACCCACGCAACTTGCAAACAGCTGTTTCAGCGACTAAAA
AATTTGGAGGGCATGCTCAAGTTGCCATTTCTTACACGACAAGTCCGGTACATACCATTGACTACTTTGTTGAATTGGCG
AAAGCTTACCAAGCTATCGGAGCGGACTCCATTTGTATCAAAGACATGGCTGGTGTCTTAACTCCTGAAATAGGTTACCA
ATTGGTCAAATGTATCAAGGAGAATACAACTATCCCTCTTGAGGTTCATACCCATGCTACCAGTGGTATTTCAGAAATGA
CTTACTTAAAAGTAGCAGAAGCAGGAGCTGATATTATTGATACGGCGATTTCCTCTTTTTCAGGGGGAACCAGTCAGCCT
GCCACAGAGTCAATGGCGATTGCCTTGACGGATTTAGGCTTTGACACAGGCTTGGATATGCAAGAGGTGGCCAAAGTTGC
AGAATATTTCAACACTATTCGTGACCACTATCGAGAAATAGGGATTTTAAATCCTAAAGTCAAAGATACTGAGCCAAAAA
CATTGATTTACCAAGTTCCAGGTGGCATGCTATCAAACCTATTGAGCCAATTAACCGAGCAAGGCCTAACTGATAAATAC
GAAGAAGTCTTAGCAGAAGTACCTAAAGTAAGGGCTGATCTTGGGTATCCGCCACTCGTAACGCCATTATCACAAATGGT
TGGCACACAGGCCTTGATGAATATCATCTCAGGGGAACGTTACAAGGTAGTTCCAAATGAAATCAAAGACTATGTTCGAG
GGCTATACGGTCAATCACCAGCGCCATTAGCAGAAGGTATCAAAGAGAAAATCATTGGTGACGAAGCAGTTATTACTTGC
AGACCAGCCGACCTAATCGAGCCTCAAATGATTTATCTACGTGACGAGATTGCTCCATACGCTCATTCAGAAGAAGACGT
GTTAAGCTATGCAAGCTTCCCGCAACAAGCTAGAGATTTCTTGGGACGCCGTGAAGATCCTTTCTATGATGTTCCGGTTC
AAGAAGTTACTGTACAACTGGACATTCAAGACTAA

Upstream 100 bases:

>100_bases
AGGAGATGCAGACTGCTATTTCAAAATTACTCCATTCATTTTTCAATAAAGACAACCAATCATCGTCATCAGATAAGACA
GGTTGATGAGGAGAACAATT

Downstream 100 bases:

>100_bases
CAGAGAATGACTAATCCTCTTAACCATTAATCTCCTTGGTGAAACGCATTTGCTGTAAGAACAATACCAAAAACCTACCT
GATTTTGATAATCAGATAGG

Product: oxaloacetate decarboxylase

Products: NA

Alternate protein names: Pyruvic carboxylase B [H]

Number of amino acids: Translated: 464; Mature: 463

Protein sequence:

>464_residues
MTKIRITETVLRDGQQSQIATRMTTKEMIPILETLDNAGYHALEMWGGATFDSCLRFLNEDPWERLRAIRKAVKKTKLQM
LLRGQNLLGYRNYADDVVRSFIQKSIENGIDIVRIFDALNDPRNLQTAVSATKKFGGHAQVAISYTTSPVHTIDYFVELA
KAYQAIGADSICIKDMAGVLTPEIGYQLVKCIKENTTIPLEVHTHATSGISEMTYLKVAEAGADIIDTAISSFSGGTSQP
ATESMAIALTDLGFDTGLDMQEVAKVAEYFNTIRDHYREIGILNPKVKDTEPKTLIYQVPGGMLSNLLSQLTEQGLTDKY
EEVLAEVPKVRADLGYPPLVTPLSQMVGTQALMNIISGERYKVVPNEIKDYVRGLYGQSPAPLAEGIKEKIIGDEAVITC
RPADLIEPQMIYLRDEIAPYAHSEEDVLSYASFPQQARDFLGRREDPFYDVPVQEVTVQLDIQD

Sequences:

>Translated_464_residues
MTKIRITETVLRDGQQSQIATRMTTKEMIPILETLDNAGYHALEMWGGATFDSCLRFLNEDPWERLRAIRKAVKKTKLQM
LLRGQNLLGYRNYADDVVRSFIQKSIENGIDIVRIFDALNDPRNLQTAVSATKKFGGHAQVAISYTTSPVHTIDYFVELA
KAYQAIGADSICIKDMAGVLTPEIGYQLVKCIKENTTIPLEVHTHATSGISEMTYLKVAEAGADIIDTAISSFSGGTSQP
ATESMAIALTDLGFDTGLDMQEVAKVAEYFNTIRDHYREIGILNPKVKDTEPKTLIYQVPGGMLSNLLSQLTEQGLTDKY
EEVLAEVPKVRADLGYPPLVTPLSQMVGTQALMNIISGERYKVVPNEIKDYVRGLYGQSPAPLAEGIKEKIIGDEAVITC
RPADLIEPQMIYLRDEIAPYAHSEEDVLSYASFPQQARDFLGRREDPFYDVPVQEVTVQLDIQD
>Mature_463_residues
TKIRITETVLRDGQQSQIATRMTTKEMIPILETLDNAGYHALEMWGGATFDSCLRFLNEDPWERLRAIRKAVKKTKLQML
LRGQNLLGYRNYADDVVRSFIQKSIENGIDIVRIFDALNDPRNLQTAVSATKKFGGHAQVAISYTTSPVHTIDYFVELAK
AYQAIGADSICIKDMAGVLTPEIGYQLVKCIKENTTIPLEVHTHATSGISEMTYLKVAEAGADIIDTAISSFSGGTSQPA
TESMAIALTDLGFDTGLDMQEVAKVAEYFNTIRDHYREIGILNPKVKDTEPKTLIYQVPGGMLSNLLSQLTEQGLTDKYE
EVLAEVPKVRADLGYPPLVTPLSQMVGTQALMNIISGERYKVVPNEIKDYVRGLYGQSPAPLAEGIKEKIIGDEAVITCR
PADLIEPQMIYLRDEIAPYAHSEEDVLSYASFPQQARDFLGRREDPFYDVPVQEVTVQLDIQD

Specific function: Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second [H]

COG id: COG5016

COG function: function code C; Pyruvate/oxaloacetate carboxyltransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 carboxyltransferase domain [H]

Homologues:

Organism=Homo sapiens, GI106049528, Length=451, Percent_Identity=32.3725055432373, Blast_Score=228, Evalue=7e-60,
Organism=Homo sapiens, GI106049295, Length=451, Percent_Identity=32.3725055432373, Blast_Score=228, Evalue=7e-60,
Organism=Homo sapiens, GI106049292, Length=451, Percent_Identity=32.3725055432373, Blast_Score=228, Evalue=7e-60,
Organism=Caenorhabditis elegans, GI17562816, Length=483, Percent_Identity=33.5403726708075, Blast_Score=254, Evalue=9e-68,
Organism=Saccharomyces cerevisiae, GI6321376, Length=461, Percent_Identity=33.1887201735358, Blast_Score=253, Evalue=6e-68,
Organism=Saccharomyces cerevisiae, GI6319695, Length=461, Percent_Identity=32.9718004338395, Blast_Score=251, Evalue=2e-67,
Organism=Drosophila melanogaster, GI24652212, Length=461, Percent_Identity=34.70715835141, Blast_Score=250, Evalue=1e-66,
Organism=Drosophila melanogaster, GI24652210, Length=461, Percent_Identity=34.70715835141, Blast_Score=250, Evalue=1e-66,
Organism=Drosophila melanogaster, GI24652214, Length=461, Percent_Identity=34.70715835141, Blast_Score=250, Evalue=1e-66,
Organism=Drosophila melanogaster, GI19921944, Length=461, Percent_Identity=34.70715835141, Blast_Score=250, Evalue=1e-66,
Organism=Drosophila melanogaster, GI24652216, Length=461, Percent_Identity=34.70715835141, Blast_Score=250, Evalue=1e-66,
Organism=Drosophila melanogaster, GI281363050, Length=461, Percent_Identity=34.70715835141, Blast_Score=250, Evalue=1e-66,
Organism=Drosophila melanogaster, GI24652224, Length=461, Percent_Identity=34.70715835141, Blast_Score=250, Evalue=1e-66,
Organism=Drosophila melanogaster, GI24652222, Length=461, Percent_Identity=34.70715835141, Blast_Score=250, Evalue=1e-66,
Organism=Drosophila melanogaster, GI24652220, Length=461, Percent_Identity=34.70715835141, Blast_Score=250, Evalue=1e-66,
Organism=Drosophila melanogaster, GI24652218, Length=461, Percent_Identity=34.70715835141, Blast_Score=250, Evalue=1e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001882
- InterPro:   IPR000089
- InterPro:   IPR003379
- InterPro:   IPR005776
- InterPro:   IPR000891
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00364 Biotin_lipoyl; PF00682 HMGL-like; PF02436 PYC_OADA [H]

EC number: =6.4.1.1 [H]

Molecular weight: Translated: 51611; Mature: 51479

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKIRITETVLRDGQQSQIATRMTTKEMIPILETLDNAGYHALEMWGGATFDSCLRFLNE
CCEEEEHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCHHHHHHHHCC
DPWERLRAIRKAVKKTKLQMLLRGQNLLGYRNYADDVVRSFIQKSIENGIDIVRIFDALN
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHC
DPRNLQTAVSATKKFGGHAQVAISYTTSPVHTIDYFVELAKAYQAIGADSICIKDMAGVL
CCHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHC
TPEIGYQLVKCIKENTTIPLEVHTHATSGISEMTYLKVAEAGADIIDTAISSFSGGTSQP
CHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCC
ATESMAIALTDLGFDTGLDMQEVAKVAEYFNTIRDHYREIGILNPKVKDTEPKTLIYQVP
CCHHHEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECC
GGMLSNLLSQLTEQGLTDKYEEVLAEVPKVRADLGYPPLVTPLSQMVGTQALMNIISGER
CHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCC
YKVVPNEIKDYVRGLYGQSPAPLAEGIKEKIIGDEAVITCRPADLIEPQMIYLRDEIAPY
EEECCHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCEEEEECCHHHCCCHHHHHHHHHCCC
AHSEEDVLSYASFPQQARDFLGRREDPFYDVPVQEVTVQLDIQD
CCCHHHHHHHHCCHHHHHHHCCCCCCCCCCCCHHHEEEEEEECC
>Mature Secondary Structure 
TKIRITETVLRDGQQSQIATRMTTKEMIPILETLDNAGYHALEMWGGATFDSCLRFLNE
CEEEEHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCHHHHHHHHCC
DPWERLRAIRKAVKKTKLQMLLRGQNLLGYRNYADDVVRSFIQKSIENGIDIVRIFDALN
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHC
DPRNLQTAVSATKKFGGHAQVAISYTTSPVHTIDYFVELAKAYQAIGADSICIKDMAGVL
CCHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHC
TPEIGYQLVKCIKENTTIPLEVHTHATSGISEMTYLKVAEAGADIIDTAISSFSGGTSQP
CHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCC
ATESMAIALTDLGFDTGLDMQEVAKVAEYFNTIRDHYREIGILNPKVKDTEPKTLIYQVP
CCHHHEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECC
GGMLSNLLSQLTEQGLTDKYEEVLAEVPKVRADLGYPPLVTPLSQMVGTQALMNIISGER
CHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCC
YKVVPNEIKDYVRGLYGQSPAPLAEGIKEKIIGDEAVITCRPADLIEPQMIYLRDEIAPY
EEECCHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCEEEEECCHHHCCCHHHHHHHHHCCC
AHSEEDVLSYASFPQQARDFLGRREDPFYDVPVQEVTVQLDIQD
CCCHHHHHHHHCCHHHHHHHCCCCCCCCCCCCHHHEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087; 11195096 [H]