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The map label for this gene is gid

Identifier: 71910706

GI number: 71910706

Start: 881400

End: 882746

Strand: Direct

Name: gid

Synonym: M5005_Spy_0893

Alternate gene names: 71910706

Gene position: 881400-882746 (Clockwise)

Preceding gene: 71910705

Following gene: 71910707

Centisome position: 47.94

GC content: 44.25

Gene sequence:

>1347_bases
TTGTCTCAATCAACTGCAACTTATATTAATGTTATTGGAGCTGGGCTAGCTGGTTCTGAAGCTGCCTATCAGATTGCTAA
GCGCGGTATCCCCGTTAAATTGTATGAAATGCGTGGTGTCAAAGCAACACCGCAACATAAAACCACTAATTTTGCCGAAT
TGGTCTGTTCCAACTCATTTCGTGGTGATAGCTTAACCAATGCAGTCGGTCTTCTCAAAGAAGAAATGCGGCGATTAGAC
TCCATTATTATGCGTAATGGTGAAGCTAACCGCGTACCTGCTGGGGGAGCAATGGCTGTTGACCGTGAGGGGTATGCAGA
GAGTGTCACTGCAGAGTTGGAAAATCATCCTCTCATTGAGGTCATTCGTGGTGAAATTACAGAAATCCCTGACGATGCTA
TCACGGTTATCGCGACGGGACCGCTGACTTCGGATGCCCTGGCAGAAAAAATTCACGCGCTAAATGGTGGCGACGGATTC
TATTTTTACGATGCAGCAGCGCCTATCATTGATAAATCTACCATTGATATGAGCAAGGTTTACCTTAAATCTCGCTACGA
TAAAGGCGAAGCTGCTTACCTCAACTGCCCTATGACCAAAGAAGAATTCATGGCTTTCCATGAAGCTCTGACAACCGCAG
AAGAAGCCCCGCTGAATGCCTTTGAAAAAGAAAAGTATTTTGAAGGCTGTATGCCGATTGAAGTTATGGCTAAACGTGGC
ATTAAAACCATGCTTTATGGACCTATGAAACCCGTTGGATTGGAATATCCAGATGACTATACAGGTCCTCGCGATGGAGA
ATTTAAAACGCCATATGCCGTCGTGCAATTGCGTCAAGATAATGCAGCTGGAAGCCTTTATAATATCGTTGGTTTCCAAA
CCCATCTCAAATGGGGTGAGCAAAAACGCGTTTTCCAAATGATTCCAGGGCTTGAAAATGCTGAGTTTGTCCGCTACGGC
GTCATGCATCGCAATTCCTATATGGATTCACCAAATCTTTTAACCGAAACCTTCCAATCTCGGAGCAATCCAAACCTTTT
CTTTGCAGGTCAGATGACTGGAGTTGAAGGTTATGTCGAATCAGCTGCTTCAGGTTTAGTAGCAGGAATCAATGCTGCTC
GTTTGTTCAAAAGAGAAGAAGCACTTATTTTTCCTCAGACAACAGCTATTGGGAGTTTGCCTCATTATGTGACTCATGCC
GACAGTAAGCATTTCCAACCAATGAACGTCAACTTTGGCATCATCAAAGAGTTAGAAGGCCCACGCATTCGTGACAAAAA
AGAACGTTATGAAGCTATTGCTAGTCGTGCTTTGGCAGATTTAGACACCTGCTTAGCGTCGCTTTAA

Upstream 100 bases:

>100_bases
CTTTCTTCTTATTTCTGTTATAATAAGTCAGGTAACTACATTAGAAAGATTTGTGGGTCTGTCATCTAAGAGATGTCATT
CCCGAAGAGGTATTAGTGAC

Downstream 100 bases:

>100_bases
CACTAAAAAAGAGTTCCTTTTCAGTTGAGTTGCTGAAGAGGACTTTTTTACTTGCTGATTTGAGCGCTAGCTATGCAAAA
GAATGCACAAAAAATACATG

Product: tRNA (uracil-5-)-methyltransferase Gid

Products: NA

Alternate protein names: Folate-dependent tRNA (uracil-5-)-methyltransferase; Folate-dependent tRNA(M-5-U54)-methyltransferase

Number of amino acids: Translated: 448; Mature: 447

Protein sequence:

>448_residues
MSQSTATYINVIGAGLAGSEAAYQIAKRGIPVKLYEMRGVKATPQHKTTNFAELVCSNSFRGDSLTNAVGLLKEEMRRLD
SIIMRNGEANRVPAGGAMAVDREGYAESVTAELENHPLIEVIRGEITEIPDDAITVIATGPLTSDALAEKIHALNGGDGF
YFYDAAAPIIDKSTIDMSKVYLKSRYDKGEAAYLNCPMTKEEFMAFHEALTTAEEAPLNAFEKEKYFEGCMPIEVMAKRG
IKTMLYGPMKPVGLEYPDDYTGPRDGEFKTPYAVVQLRQDNAAGSLYNIVGFQTHLKWGEQKRVFQMIPGLENAEFVRYG
VMHRNSYMDSPNLLTETFQSRSNPNLFFAGQMTGVEGYVESAASGLVAGINAARLFKREEALIFPQTTAIGSLPHYVTHA
DSKHFQPMNVNFGIIKELEGPRIRDKKERYEAIASRALADLDTCLASL

Sequences:

>Translated_448_residues
MSQSTATYINVIGAGLAGSEAAYQIAKRGIPVKLYEMRGVKATPQHKTTNFAELVCSNSFRGDSLTNAVGLLKEEMRRLD
SIIMRNGEANRVPAGGAMAVDREGYAESVTAELENHPLIEVIRGEITEIPDDAITVIATGPLTSDALAEKIHALNGGDGF
YFYDAAAPIIDKSTIDMSKVYLKSRYDKGEAAYLNCPMTKEEFMAFHEALTTAEEAPLNAFEKEKYFEGCMPIEVMAKRG
IKTMLYGPMKPVGLEYPDDYTGPRDGEFKTPYAVVQLRQDNAAGSLYNIVGFQTHLKWGEQKRVFQMIPGLENAEFVRYG
VMHRNSYMDSPNLLTETFQSRSNPNLFFAGQMTGVEGYVESAASGLVAGINAARLFKREEALIFPQTTAIGSLPHYVTHA
DSKHFQPMNVNFGIIKELEGPRIRDKKERYEAIASRALADLDTCLASL
>Mature_447_residues
SQSTATYINVIGAGLAGSEAAYQIAKRGIPVKLYEMRGVKATPQHKTTNFAELVCSNSFRGDSLTNAVGLLKEEMRRLDS
IIMRNGEANRVPAGGAMAVDREGYAESVTAELENHPLIEVIRGEITEIPDDAITVIATGPLTSDALAEKIHALNGGDGFY
FYDAAAPIIDKSTIDMSKVYLKSRYDKGEAAYLNCPMTKEEFMAFHEALTTAEEAPLNAFEKEKYFEGCMPIEVMAKRGI
KTMLYGPMKPVGLEYPDDYTGPRDGEFKTPYAVVQLRQDNAAGSLYNIVGFQTHLKWGEQKRVFQMIPGLENAEFVRYGV
MHRNSYMDSPNLLTETFQSRSNPNLFFAGQMTGVEGYVESAASGLVAGINAARLFKREEALIFPQTTAIGSLPHYVTHAD
SKHFQPMNVNFGIIKELEGPRIRDKKERYEAIASRALADLDTCLASL

Specific function: Catalyzes the folate-dependent formation of 5-methyl- uridine at position 54 (M-5-U54) in all tRNAs

COG id: COG1206

COG function: function code J; NAD(FAD)-utilizing enzyme possibly involved in translation

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family. TrmFO subfamily

Homologues:

Organism=Escherichia coli, GI2367273, Length=90, Percent_Identity=42.2222222222222, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMFO_STRP1 (Q99ZL9)

Other databases:

- EMBL:   AE004092
- EMBL:   CP000017
- RefSeq:   NP_269319.1
- RefSeq:   YP_282256.1
- ProteinModelPortal:   Q99ZL9
- EnsemblBacteria:   EBSTRT00000001037
- EnsemblBacteria:   EBSTRT00000028231
- GeneID:   3572024
- GeneID:   901285
- GenomeReviews:   AE004092_GR
- GenomeReviews:   CP000017_GR
- KEGG:   spy:SPy_1173
- KEGG:   spz:M5005_Spy_0893
- GeneTree:   EBGT00050000026916
- HOGENOM:   HBG285041
- OMA:   FEGCLPI
- ProtClustDB:   PRK05335
- BioCyc:   SPYO160490:SPY1173-MONOMER
- BioCyc:   SPYO293653:M5005_SPY0893-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01037
- InterPro:   IPR004417
- InterPro:   IPR002218
- InterPro:   IPR020595
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.40.50.720
- TIGRFAMs:   TIGR00137

Pfam domain/function: PF01134 GIDA

EC number: =2.1.1.74

Molecular weight: Translated: 49425; Mature: 49294

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: PS01280 GIDA_1; PS01281 GIDA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQSTATYINVIGAGLAGSEAAYQIAKRGIPVKLYEMRGVKATPQHKTTNFAELVCSNSF
CCCCHHHHHHHHCCCCCCCHHHHHHHHCCCCEEEEEECCCCCCCCCCCCHHHHHHHCCCC
RGDSLTNAVGLLKEEMRRLDSIIMRNGEANRVPAGGAMAVDREGYAESVTAELENHPLIE
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCCCHHHHHHHHCCCCHHH
VIRGEITEIPDDAITVIATGPLTSDALAEKIHALNGGDGFYFYDAAAPIIDKSTIDMSKV
HHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCHHHHH
YLKSRYDKGEAAYLNCPMTKEEFMAFHEALTTAEEAPLNAFEKEKYFEGCMPIEVMAKRG
HHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHCC
IKTMLYGPMKPVGLEYPDDYTGPRDGEFKTPYAVVQLRQDNAAGSLYNIVGFQTHLKWGE
CCEECCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCH
QKRVFQMIPGLENAEFVRYGVMHRNSYMDSPNLLTETFQSRSNPNLFFAGQMTGVEGYVE
HHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCEEEEEECCCHHHHHH
SAASGLVAGINAARLFKREEALIFPQTTAIGSLPHYVTHADSKHFQPMNVNFGIIKELEG
HHHHHHHHCCHHHHHHHHCCCEEECCHHHHCCCCHHHCCCCCCCCCCCCCCCCEEECCCC
PRIRDKKERYEAIASRALADLDTCLASL
CCCCCHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SQSTATYINVIGAGLAGSEAAYQIAKRGIPVKLYEMRGVKATPQHKTTNFAELVCSNSF
CCCHHHHHHHHCCCCCCCHHHHHHHHCCCCEEEEEECCCCCCCCCCCCHHHHHHHCCCC
RGDSLTNAVGLLKEEMRRLDSIIMRNGEANRVPAGGAMAVDREGYAESVTAELENHPLIE
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCCCHHHHHHHHCCCCHHH
VIRGEITEIPDDAITVIATGPLTSDALAEKIHALNGGDGFYFYDAAAPIIDKSTIDMSKV
HHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCHHHHH
YLKSRYDKGEAAYLNCPMTKEEFMAFHEALTTAEEAPLNAFEKEKYFEGCMPIEVMAKRG
HHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHCC
IKTMLYGPMKPVGLEYPDDYTGPRDGEFKTPYAVVQLRQDNAAGSLYNIVGFQTHLKWGE
CCEECCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCH
QKRVFQMIPGLENAEFVRYGVMHRNSYMDSPNLLTETFQSRSNPNLFFAGQMTGVEGYVE
HHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCEEEEEECCCHHHHHH
SAASGLVAGINAARLFKREEALIFPQTTAIGSLPHYVTHADSKHFQPMNVNFGIIKELEG
HHHHHHHHCCHHHHHHHHCCCEEECCHHHHCCCCHHHCCCCCCCCCCCCCCCCEEECCCC
PRIRDKKERYEAIASRALADLDTCLASL
CCCCCHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11296296