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The map label for this gene is gyrA

Identifier: 71910687

GI number: 71910687

Start: 865365

End: 867851

Strand: Direct

Name: gyrA

Synonym: M5005_Spy_0874

Alternate gene names: 71910687

Gene position: 865365-867851 (Clockwise)

Preceding gene: 71910685

Following gene: 71910688

Centisome position: 47.07

GC content: 40.01

Gene sequence:

>2487_bases
ATGCAAGATCGAAATTTAATTGACGTCAATCTGACAAGTGAAATGAAAACGAGTTTCATTGACTATGCCATGAGTGTCAT
TGTGGCAAGAGCTTTGCCAGATGTGCGTGATGGTCTGAAACCTGTGCATCGTCGTATTCTCTATGGGATGAATGAACTCG
GTGTGACACCTGATAAACCGCATAAAAAATCTGCTCGTATTACGGGTGATGTCATGGGTAAATATCACCCGCATGGGGAT
TCTTCTATTTATGAAGCTATGGTTCGTATGGCTCAGTGGTGGAGTTATCGCCATATGCTTGTTGATGGGCATGGCAATTT
TGGTTCTATGGATGGTGATGGTGCTGCCGCTCAACGTTATACTGAAGCGCGCATGAGTAAAATTGCGCTTGAATTATTAA
GGGATATCAATAAAAATACCGTTAATTTTCAAGATAACTACGATGGAAGCGAAAGAGAACCAGTAGTATTACCAGCTCGC
TTTCCTAATTTATTAGTCAATGGAGCGACAGGGATTGCCGTTGGGATGGCAACTAACATTCCGCCTCATAATTTGGCAGA
GTCTATTGATGCTGTCAAGATGGTAATGGAACATCCAGACTGTACAACAAGAGAGTTGATGGAAGTGATCCCTGGACCTG
ACTTTCCGACTGGTGCCCTTGTTATGGGGCGCTCAGGTATTCATAGGGCTTATGATACTGGTAAAGGTTCTATCGTTTTA
CGCTCTCGAACAGAAATTGAAACAACTCAAACAGGTCGGGAACGTATTGTGGTTACTGAATTTCCCTATGGCGTCAATAA
AACGAAAGTGCATGAACACATTGTTCGTTTAGCACAAGAAAAAAGACTAGAAGGTATTACAGCAGTTCGTGACGAATCTA
GCCGTGAAGGAGTGCGTTTTGTTATCGAGATTCGTCGAGAGGCATCCGCGACAGTTATCTTAAATAACCTCTTTAAATTA
ACAAGTCTTCAGACGAATTTCAGTTTTAATATGTTGGCTATTGAAAATGGTGTGCCTAAAATATTGTCACTCAGACAAAT
CATTGATAATTACATTAGTCATCAAAAAGAAGTTATTATACGTCGCACCCGATTTGATAAAGATAAAGCTGAAGCCAGAG
CTCATATTTTAGAAGGCTTACTGATTGCCCTTGATCATTTAGACGAAGTGATTGCTATTATTCGTAACAGCGAGACAGAT
GTCATTGCTCAGACAGAATTGATGTCACGTTTCGACTTGTCTGAACGCCAAAGCCAAGCTATTTTGGATATGCGTTTGCG
TCGTTTGACTGGTTTGGAACGTGATAAGATTCAATCAGAATATGATGATTTGTTGGCCTTGATTGCTGACTTATCTGATA
TTTTAGCCAAACCAGAACGTATTATCACTATCATCAAAGAAGAAATGGATGAGATCAAGCGTAAATACGCTAACCCACGC
CGTACAGAATTAATGGTCGGTGAAGTCCTTTCTCTAGAAGATGAAGATCTAATTGAAGAAGAAGATGTTTTGATTACCTT
GTCAAATAAAGGTTATATCAAGCGTCTTGCTCAAGATGAATTTCGTGCCCAGAAGCGTGGTGGACGTGGCGTCCAAGGAA
CTGGAGTCAACAATGACGACTTTGTACGTGAATTGATATCAACAAGTACCCATGATACTTTGCTCTTCTTTACGAATTTT
GGGCGTGTCTATCGGTTGAAAGCTTACGAAATTCCAGAATATGGTCGTACAGCAAAAGGTCTTCCAATTGTTAATCTATT
GAAATTAGAAGATGGTGAAACCATTCAAACTATTATCAACGCACGTAAGGAAGAAACAGCAGGTAAATCCTTCTTCTTTA
CAACGAAACAAGGCATTGTCAAACGTACAGAAGTTTCAGAATTTAATAATATCCGTCAAAACGGTCTCCGTGCTCTTAAG
TTGAAAGAGGGAGATCAGCTGATTAACGTCCTTTTGACAAGCGGTCAGGATGACATTATCATTGGAACTCATTCCGGATA
TTCTGTTCGTTTTAATGAAGCAAGCATCCGCAACATGGGCCGATCAGCTACGGGTGTTCGCGGGGTTAAGCTTCGTGAAG
ATGATCGCGTTGTTGGCGCGTCACGTATTCAAGATAACCAAGAAGTTCTTGTAATTACCGAAAATGGCTTTGGTAAGAGA
ACAAGTGCTACAGACTATCCTACAAAAGGTAGGGGGGGAAAAGGAATAAAAACAGCTAATATTACCCCTAAAAATGGTCA
ATTGGCTGGATTGGTTACCGTAGATGGTACAGAAGATATCATGGTGATTACAAATAAAGGTGTCATTATTCGCACAAATG
TTGCAAATATTTCGCAAACAGGTCGTGCTACTTTGGGTGTTAAAATTATGAAACTAGATGCTGATGCTAAGATTGTAACA
TTTACCTTGGTTCAGCCTGAAGACAGTAGTATCGCTGAAATCAATACTGATCGTGAAAATAGTATTTCAAAAAATAAAGA
TAATTAA

Upstream 100 bases:

>100_bases
ATAATGAAAATAGTTTACTGATAACGCTTCCAATAAGAAAAAATAAGGAATTTATGGTATAATGAAATCTAGTATTGACT
AGAAAAGGAGCATTCCTTAA

Downstream 100 bases:

>100_bases
TCATAATAAGGAGTAAAAAATGGTAAAAAAACAAAAGCGTCGAAAAATAAAATCCATGTCTTGGGCAAGAAAACTACTAA
TAGCTGTACTTTTAATTCTA

Product: DNA gyrase subunit A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 828; Mature: 828

Protein sequence:

>828_residues
MQDRNLIDVNLTSEMKTSFIDYAMSVIVARALPDVRDGLKPVHRRILYGMNELGVTPDKPHKKSARITGDVMGKYHPHGD
SSIYEAMVRMAQWWSYRHMLVDGHGNFGSMDGDGAAAQRYTEARMSKIALELLRDINKNTVNFQDNYDGSEREPVVLPAR
FPNLLVNGATGIAVGMATNIPPHNLAESIDAVKMVMEHPDCTTRELMEVIPGPDFPTGALVMGRSGIHRAYDTGKGSIVL
RSRTEIETTQTGRERIVVTEFPYGVNKTKVHEHIVRLAQEKRLEGITAVRDESSREGVRFVIEIRREASATVILNNLFKL
TSLQTNFSFNMLAIENGVPKILSLRQIIDNYISHQKEVIIRRTRFDKDKAEARAHILEGLLIALDHLDEVIAIIRNSETD
VIAQTELMSRFDLSERQSQAILDMRLRRLTGLERDKIQSEYDDLLALIADLSDILAKPERIITIIKEEMDEIKRKYANPR
RTELMVGEVLSLEDEDLIEEEDVLITLSNKGYIKRLAQDEFRAQKRGGRGVQGTGVNNDDFVRELISTSTHDTLLFFTNF
GRVYRLKAYEIPEYGRTAKGLPIVNLLKLEDGETIQTIINARKEETAGKSFFFTTKQGIVKRTEVSEFNNIRQNGLRALK
LKEGDQLINVLLTSGQDDIIIGTHSGYSVRFNEASIRNMGRSATGVRGVKLREDDRVVGASRIQDNQEVLVITENGFGKR
TSATDYPTKGRGGKGIKTANITPKNGQLAGLVTVDGTEDIMVITNKGVIIRTNVANISQTGRATLGVKIMKLDADAKIVT
FTLVQPEDSSIAEINTDRENSISKNKDN

Sequences:

>Translated_828_residues
MQDRNLIDVNLTSEMKTSFIDYAMSVIVARALPDVRDGLKPVHRRILYGMNELGVTPDKPHKKSARITGDVMGKYHPHGD
SSIYEAMVRMAQWWSYRHMLVDGHGNFGSMDGDGAAAQRYTEARMSKIALELLRDINKNTVNFQDNYDGSEREPVVLPAR
FPNLLVNGATGIAVGMATNIPPHNLAESIDAVKMVMEHPDCTTRELMEVIPGPDFPTGALVMGRSGIHRAYDTGKGSIVL
RSRTEIETTQTGRERIVVTEFPYGVNKTKVHEHIVRLAQEKRLEGITAVRDESSREGVRFVIEIRREASATVILNNLFKL
TSLQTNFSFNMLAIENGVPKILSLRQIIDNYISHQKEVIIRRTRFDKDKAEARAHILEGLLIALDHLDEVIAIIRNSETD
VIAQTELMSRFDLSERQSQAILDMRLRRLTGLERDKIQSEYDDLLALIADLSDILAKPERIITIIKEEMDEIKRKYANPR
RTELMVGEVLSLEDEDLIEEEDVLITLSNKGYIKRLAQDEFRAQKRGGRGVQGTGVNNDDFVRELISTSTHDTLLFFTNF
GRVYRLKAYEIPEYGRTAKGLPIVNLLKLEDGETIQTIINARKEETAGKSFFFTTKQGIVKRTEVSEFNNIRQNGLRALK
LKEGDQLINVLLTSGQDDIIIGTHSGYSVRFNEASIRNMGRSATGVRGVKLREDDRVVGASRIQDNQEVLVITENGFGKR
TSATDYPTKGRGGKGIKTANITPKNGQLAGLVTVDGTEDIMVITNKGVIIRTNVANISQTGRATLGVKIMKLDADAKIVT
FTLVQPEDSSIAEINTDRENSISKNKDN
>Mature_828_residues
MQDRNLIDVNLTSEMKTSFIDYAMSVIVARALPDVRDGLKPVHRRILYGMNELGVTPDKPHKKSARITGDVMGKYHPHGD
SSIYEAMVRMAQWWSYRHMLVDGHGNFGSMDGDGAAAQRYTEARMSKIALELLRDINKNTVNFQDNYDGSEREPVVLPAR
FPNLLVNGATGIAVGMATNIPPHNLAESIDAVKMVMEHPDCTTRELMEVIPGPDFPTGALVMGRSGIHRAYDTGKGSIVL
RSRTEIETTQTGRERIVVTEFPYGVNKTKVHEHIVRLAQEKRLEGITAVRDESSREGVRFVIEIRREASATVILNNLFKL
TSLQTNFSFNMLAIENGVPKILSLRQIIDNYISHQKEVIIRRTRFDKDKAEARAHILEGLLIALDHLDEVIAIIRNSETD
VIAQTELMSRFDLSERQSQAILDMRLRRLTGLERDKIQSEYDDLLALIADLSDILAKPERIITIIKEEMDEIKRKYANPR
RTELMVGEVLSLEDEDLIEEEDVLITLSNKGYIKRLAQDEFRAQKRGGRGVQGTGVNNDDFVRELISTSTHDTLLFFTNF
GRVYRLKAYEIPEYGRTAKGLPIVNLLKLEDGETIQTIINARKEETAGKSFFFTTKQGIVKRTEVSEFNNIRQNGLRALK
LKEGDQLINVLLTSGQDDIIIGTHSGYSVRFNEASIRNMGRSATGVRGVKLREDDRVVGASRIQDNQEVLVITENGFGKR
TSATDYPTKGRGGKGIKTANITPKNGQLAGLVTVDGTEDIMVITNKGVIIRTNVANISQTGRATLGVKIMKLDADAKIVT
FTLVQPEDSSIAEINTDRENSISKNKDN

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings

COG id: COG0188

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), A subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the topoisomerase gyrA/parC subunit family

Homologues:

Organism=Homo sapiens, GI19913406, Length=338, Percent_Identity=25.4437869822485, Blast_Score=78, Evalue=4e-14,
Organism=Escherichia coli, GI1788562, Length=841, Percent_Identity=47.5624256837099, Blast_Score=792, Evalue=0.0,
Organism=Escherichia coli, GI1789396, Length=593, Percent_Identity=37.2681281618887, Blast_Score=381, Evalue=1e-106,
Organism=Caenorhabditis elegans, GI212645845, Length=477, Percent_Identity=22.8511530398323, Blast_Score=73, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17535065, Length=174, Percent_Identity=31.6091954022989, Blast_Score=70, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6324241, Length=369, Percent_Identity=23.8482384823848, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI17136538, Length=413, Percent_Identity=23.2445520581114, Blast_Score=75, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GYRA_STRP1 (Q9L7Q5)

Other databases:

- EMBL:   AF220945
- EMBL:   AE004092
- EMBL:   CP000017
- RefSeq:   NP_269303.1
- RefSeq:   YP_282237.1
- ProteinModelPortal:   Q9L7Q5
- EnsemblBacteria:   EBSTRT00000001522
- EnsemblBacteria:   EBSTRT00000029015
- GeneID:   3572044
- GeneID:   901268
- GenomeReviews:   AE004092_GR
- GenomeReviews:   CP000017_GR
- KEGG:   spy:SPy_1152
- KEGG:   spz:M5005_Spy_0874
- GeneTree:   EBGT00050000028052
- HOGENOM:   HBG635183
- OMA:   ELGNDWN
- ProtClustDB:   PRK05560
- BioCyc:   SPYO160490:SPY1152-MONOMER
- BioCyc:   SPYO293653:M5005_SPY0874-MONOMER
- GO:   GO:0005694
- InterPro:   IPR020899
- InterPro:   IPR005743
- InterPro:   IPR006691
- InterPro:   IPR002205
- InterPro:   IPR013758
- InterPro:   IPR013757
- InterPro:   IPR013760
- Gene3D:   G3DSA:3.30.1360.40
- Gene3D:   G3DSA:3.90.199.10
- Gene3D:   G3DSA:1.10.268.10
- SMART:   SM00434
- TIGRFAMs:   TIGR01063

Pfam domain/function: PF03989 DNA_gyraseA_C; PF00521 DNA_topoisoIV; SSF56719 Topo_IIA_cen

EC number: =5.99.1.3

Molecular weight: Translated: 92705; Mature: 92705

Theoretical pI: Translated: 7.13; Mature: 7.13

Prosite motif: PS00572 GLYCOSYL_HYDROL_F1_1

Important sites: ACT_SITE 120-120

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQDRNLIDVNLTSEMKTSFIDYAMSVIVARALPDVRDGLKPVHRRILYGMNELGVTPDKP
CCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCHHHCCCCCCCC
HKKSARITGDVMGKYHPHGDSSIYEAMVRMAQWWSYRHMLVDGHGNFGSMDGDGAAAQRY
CCCCCCEEHHHCCCCCCCCCCHHHHHHHHHHHHHCEEEEEEECCCCCCCCCCCCHHHHHH
TEARMSKIALELLRDINKNTVNFQDNYDGSEREPVVLPARFPNLLVNGATGIAVGMATNI
HHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCCEEEECCCCCCEEECCCEEEEEEECCC
PPHNLAESIDAVKMVMEHPDCTTRELMEVIPGPDFPTGALVMGRSGIHRAYDTGKGSIVL
CCHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCEEEECCCCCCHHHCCCCCCEEE
RSRTEIETTQTGRERIVVTEFPYGVNKTKVHEHIVRLAQEKRLEGITAVRDESSREGVRF
ECCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCEEE
VIEIRREASATVILNNLFKLTSLQTNFSFNMLAIENGVPKILSLRQIIDNYISHQKEVII
EEEEHHCCCHHHHHHHHHHHHEEECCCCEEEEEECCCCHHHHHHHHHHHHHHCCHHHHHH
RRTRFDKDKAEARAHILEGLLIALDHLDEVIAIIRNSETDVIAQTELMSRFDLSERQSQA
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHHH
ILDMRLRRLTGLERDKIQSEYDDLLALIADLSDILAKPERIITIIKEEMDEIKRKYANPR
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCC
RTELMVGEVLSLEDEDLIEEEDVLITLSNKGYIKRLAQDEFRAQKRGGRGVQGTGVNNDD
HHHHHHHHHHCCCCHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHH
FVRELISTSTHDTLLFFTNFGRVYRLKAYEIPEYGRTAKGLPIVNLLKLEDGETIQTIIN
HHHHHHHCCCCCEEEEEECCCEEEEEEEEECCCCCCCCCCCCEEEEEEECCCCHHHHHHH
ARKEETAGKSFFFTTKQGIVKRTEVSEFNNIRQNGLRALKLKEGDQLINVLLTSGQDDII
HHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHCCCCCCEE
IGTHSGYSVRFNEASIRNMGRSATGVRGVKLREDDRVVGASRIQDNQEVLVITENGFGKR
EECCCCCEEEECCHHHHHCCCCCCCCCCEEECCCCCEECHHHCCCCCEEEEEECCCCCCC
TSATDYPTKGRGGKGIKTANITPKNGQLAGLVTVDGTEDIMVITNKGVIIRTNVANISQT
CCCCCCCCCCCCCCCEEECCCCCCCCCEEEEEEECCCCCEEEEECCCEEEEECCCCCCCC
GRATLGVKIMKLDADAKIVTFTLVQPEDSSIAEINTDRENSISKNKDN
CCEEEEEEEEEECCCCEEEEEEEECCCCCCCEEECCCCCCCCCCCCCC
>Mature Secondary Structure
MQDRNLIDVNLTSEMKTSFIDYAMSVIVARALPDVRDGLKPVHRRILYGMNELGVTPDKP
CCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCHHHCCCCCCCC
HKKSARITGDVMGKYHPHGDSSIYEAMVRMAQWWSYRHMLVDGHGNFGSMDGDGAAAQRY
CCCCCCEEHHHCCCCCCCCCCHHHHHHHHHHHHHCEEEEEEECCCCCCCCCCCCHHHHHH
TEARMSKIALELLRDINKNTVNFQDNYDGSEREPVVLPARFPNLLVNGATGIAVGMATNI
HHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCCEEEECCCCCCEEECCCEEEEEEECCC
PPHNLAESIDAVKMVMEHPDCTTRELMEVIPGPDFPTGALVMGRSGIHRAYDTGKGSIVL
CCHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCEEEECCCCCCHHHCCCCCCEEE
RSRTEIETTQTGRERIVVTEFPYGVNKTKVHEHIVRLAQEKRLEGITAVRDESSREGVRF
ECCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCEEE
VIEIRREASATVILNNLFKLTSLQTNFSFNMLAIENGVPKILSLRQIIDNYISHQKEVII
EEEEHHCCCHHHHHHHHHHHHEEECCCCEEEEEECCCCHHHHHHHHHHHHHHCCHHHHHH
RRTRFDKDKAEARAHILEGLLIALDHLDEVIAIIRNSETDVIAQTELMSRFDLSERQSQA
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHHH
ILDMRLRRLTGLERDKIQSEYDDLLALIADLSDILAKPERIITIIKEEMDEIKRKYANPR
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCC
RTELMVGEVLSLEDEDLIEEEDVLITLSNKGYIKRLAQDEFRAQKRGGRGVQGTGVNNDD
HHHHHHHHHHCCCCHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHH
FVRELISTSTHDTLLFFTNFGRVYRLKAYEIPEYGRTAKGLPIVNLLKLEDGETIQTIIN
HHHHHHHCCCCCEEEEEECCCEEEEEEEEECCCCCCCCCCCCEEEEEEECCCCHHHHHHH
ARKEETAGKSFFFTTKQGIVKRTEVSEFNNIRQNGLRALKLKEGDQLINVLLTSGQDDII
HHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHCCCCCCEE
IGTHSGYSVRFNEASIRNMGRSATGVRGVKLREDDRVVGASRIQDNQEVLVITENGFGKR
EECCCCCEEEECCHHHHHCCCCCCCCCCEEECCCCCEECHHHCCCCCEEEEEECCCCCCC
TSATDYPTKGRGGKGIKTANITPKNGQLAGLVTVDGTEDIMVITNKGVIIRTNVANISQT
CCCCCCCCCCCCCCCEEECCCCCCCCCEEEEEEECCCCCEEEEECCCEEEEECCCCCCCC
GRATLGVKIMKLDADAKIVTFTLVQPEDSSIAEINTDRENSISKNKDN
CCEEEEEEEEEECCCCEEEEEEEECCCCCCCEEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11296296