Click here to switch to the map view.

The map label for this gene is ppnK [H]

Identifier: 71910661

GI number: 71910661

Start: 840574

End: 841410

Strand: Direct

Name: ppnK [H]

Synonym: M5005_Spy_0848

Alternate gene names: 71910661

Gene position: 840574-841410 (Clockwise)

Preceding gene: 71910660

Following gene: 71910662

Centisome position: 45.72

GC content: 37.4

Gene sequence:

>837_bases
ATGACACAGATGAATTATACAGGTAAGGTAAAACGAGTTGCTATTATTGCAAATGGTAAGTACCAAAGTAAACGCGTCGC
CTCCAAACTTTTCTCCGTATTTAAAGATGATCCTGATTTCTATCTTTCAAAGAAAAATCCGGATATTGTGATTTCTATTG
GCGGAGATGGGATGCTCTTATCTGCCTTTCACATGTATGAAAAAGAATTAGATAAGGTACGTTTTGTAGGAATCCACACC
GGTCATCTTGGCTTTTATACCGATTATAGGGATTTTGAAGTTGATAAATTAATTGATAATTTAAGAAAAGACAAGGGAGA
ACAAATCTCTTATCCGATTTTAAAAGTTGCTATTACTTTAGATGATGGTCGTGTGGTTAAAGCGCGTGCTTTGAATGAAG
CGACGGTTAAGCGTATTGAAAAAACGATGGTAGCAGATGTTATTATTAACCATGTCAAATTTGAAAGCTTCCGAGGTGAT
GGGATTTCAGTATCGACCCCGACAGGGAGCACAGCCTACAATAAATCTTTAGGTGGTGCTGTCTTGCATCCGACGATTGA
AGCGCTGCAATTGACGGAAATTTCCAGTCTTAATAACCGTGTCTTTAGAACCTTGGGCTCATCAATCATTATTCCCAAAA
AAGATAAGATTGAGTTAGTGCCAAAACGATTAGGAATTTATACCATTTCCATTGATAATAAAACCTATCAGTTAAAAAAT
GTGACGAAGGTGGAGTATTTTATCGACGATGAGAAAATTCATTTTGTTTCCTCTCCGAGTCATACGAGCTTTTGGGAAAG
GGTCAAGGATGCCTTTATTGGAGAGATTGACTCATGA

Upstream 100 bases:

>100_bases
TTAGACGAAGAAATGCGTAAGATTCGAGAAGATATTCGGGAGGCTCAATTATTGTTTGACCCCGTGACACGAAATTTAAG
TGATGGTGTAGGAAATAGTG

Downstream 100 bases:

>100_bases
GATTTGAATTTGTAGCAGACAAACGAATAAAAGTAAAAACCCTTTTAAAAAGTTATGATGTCTCTAAAGGACTATTGGCT
AAAATCAAATATAAGGGCGG

Product: inorganic polyphosphate/ATP-NAD kinase

Products: NA

Alternate protein names: Poly(P)/ATP NAD kinase [H]

Number of amino acids: Translated: 278; Mature: 277

Protein sequence:

>278_residues
MTQMNYTGKVKRVAIIANGKYQSKRVASKLFSVFKDDPDFYLSKKNPDIVISIGGDGMLLSAFHMYEKELDKVRFVGIHT
GHLGFYTDYRDFEVDKLIDNLRKDKGEQISYPILKVAITLDDGRVVKARALNEATVKRIEKTMVADVIINHVKFESFRGD
GISVSTPTGSTAYNKSLGGAVLHPTIEALQLTEISSLNNRVFRTLGSSIIIPKKDKIELVPKRLGIYTISIDNKTYQLKN
VTKVEYFIDDEKIHFVSSPSHTSFWERVKDAFIGEIDS

Sequences:

>Translated_278_residues
MTQMNYTGKVKRVAIIANGKYQSKRVASKLFSVFKDDPDFYLSKKNPDIVISIGGDGMLLSAFHMYEKELDKVRFVGIHT
GHLGFYTDYRDFEVDKLIDNLRKDKGEQISYPILKVAITLDDGRVVKARALNEATVKRIEKTMVADVIINHVKFESFRGD
GISVSTPTGSTAYNKSLGGAVLHPTIEALQLTEISSLNNRVFRTLGSSIIIPKKDKIELVPKRLGIYTISIDNKTYQLKN
VTKVEYFIDDEKIHFVSSPSHTSFWERVKDAFIGEIDS
>Mature_277_residues
TQMNYTGKVKRVAIIANGKYQSKRVASKLFSVFKDDPDFYLSKKNPDIVISIGGDGMLLSAFHMYEKELDKVRFVGIHTG
HLGFYTDYRDFEVDKLIDNLRKDKGEQISYPILKVAITLDDGRVVKARALNEATVKRIEKTMVADVIINHVKFESFRGDG
ISVSTPTGSTAYNKSLGGAVLHPTIEALQLTEISSLNNRVFRTLGSSIIIPKKDKIELVPKRLGIYTISIDNKTYQLKNV
TKVEYFIDDEKIHFVSSPSHTSFWERVKDAFIGEIDS

Specific function: Catalyzes the phosphorylation of NAD to NADP. Utilizes ATP and other nucleoside triphosphates as well as inorganic polyphosphate as a source of phosphorus [H]

COG id: COG0061

COG function: function code G; Predicted sugar kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD kinase family [H]

Homologues:

Organism=Escherichia coli, GI1788968, Length=152, Percent_Identity=32.2368421052632, Blast_Score=70, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6320794, Length=190, Percent_Identity=30.5263157894737, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016064
- InterPro:   IPR017438
- InterPro:   IPR017437
- InterPro:   IPR002504 [H]

Pfam domain/function: PF01513 NAD_kinase [H]

EC number: =2.7.1.23 [H]

Molecular weight: Translated: 31376; Mature: 31245

Theoretical pI: Translated: 9.69; Mature: 9.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQMNYTGKVKRVAIIANGKYQSKRVASKLFSVFKDDPDFYLSKKNPDIVISIGGDGMLL
CCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHCCCCCEEEECCCCCEEEEECCCCHHH
SAFHMYEKELDKVRFVGIHTGHLGFYTDYRDFEVDKLIDNLRKDKGEQISYPILKVAITL
HHHHHHHHHHCCEEEEEEEECCEEEECCCCCCCHHHHHHHHHHCCCCCCCCCEEEEEEEE
DDGRVVKARALNEATVKRIEKTMVADVIINHVKFESFRGDGISVSTPTGSTAYNKSLGGA
CCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHEEECCCCCCEEEECCCCCCCCCCCCCCE
VLHPTIEALQLTEISSLNNRVFRTLGSSIIIPKKDKIELVPKRLGIYTISIDNKTYQLKN
EECCHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCEEEECCCCEEEEEECCCEEEECC
VTKVEYFIDDEKIHFVSSPSHTSFWERVKDAFIGEIDS
CEEEEEEECCCEEEEEECCCCHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
TQMNYTGKVKRVAIIANGKYQSKRVASKLFSVFKDDPDFYLSKKNPDIVISIGGDGMLL
CCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHCCCCCEEEECCCCCEEEEECCCCHHH
SAFHMYEKELDKVRFVGIHTGHLGFYTDYRDFEVDKLIDNLRKDKGEQISYPILKVAITL
HHHHHHHHHHCCEEEEEEEECCEEEECCCCCCCHHHHHHHHHHCCCCCCCCCEEEEEEEE
DDGRVVKARALNEATVKRIEKTMVADVIINHVKFESFRGDGISVSTPTGSTAYNKSLGGA
CCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHEEECCCCCCEEEECCCCCCCCCCCCCCE
VLHPTIEALQLTEISSLNNRVFRTLGSSIIIPKKDKIELVPKRLGIYTISIDNKTYQLKN
EECCHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCEEEECCCCEEEEEECCCEEEECC
VTKVEYFIDDEKIHFVSSPSHTSFWERVKDAFIGEIDS
CEEEEEEECCCEEEEEECCCCHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA