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The map label for this gene is prs2

Identifier: 71910658

GI number: 71910658

Start: 838142

End: 839122

Strand: Reverse

Name: prs2

Synonym: M5005_Spy_0845

Alternate gene names: 71910658

Gene position: 839122-838142 (Counterclockwise)

Preceding gene: 71910659

Following gene: 71910657

Centisome position: 45.64

GC content: 38.02

Gene sequence:

>981_bases
ATGACTGAACGATATGCTGACAAGCAAATCAAACTGTTCTCACTCACATCAAATCTTCCAATTGCTGAAAAAATTGCTAA
AGCTGCTGGAATCCCTCTTGGAAAAATGTCTTCACGTCAATTTTCCAATGGAGAAATTATGATCAATATTGAAGAAACAG
TGCGTGGAGATGATATCTATATTATTCAATCCACTAGTTTTCCTGTCAATGATAATCTTTGGGAATTACTCATCATGATT
GATGCTTGTAAACGTGCAAGTGCTAATACCGTCAATATTGTATTGCCATACTTTGGTTACTCTCGTCAAGACCGTGTCGC
TAAGCCCCGCGAACCGATTACTGCTAAACTAGTAGCTAATATGCTGACTAAAGCTGGTATTGATCGTGTAGTGACGCTTG
ACTTACATGCTGTTCAGGTACAAGGTTTTTTTGATATTCCAGTGGATAACCTCTTTACAGTCCCTCTTTTTGCAGAACGT
TACAGTAAATTAGGCTTATCAGGTTCTGATGTTGTTGTCGTTAGCCCTAAAAATTCTGGAATTAAACGTGCTAGAAGCTT
GGCTGAATATCTTGATTCCCCGATTGCTATTATCGACTATGCACAAGATGATTCTGAACGTGAACAAGGTTATATTATTG
GTGATGTTTCTGGTAAAAAAGCCATTTTAATTGATGATATTTTAAATACTGGGAAAACCTTTGCAGAAGCTGCTAAAATT
CTTGAACGCTCAGGCGCTACTGATACTTACGCGGTCGCTAGCCATGGCTTATTTGCTGGTGGAGCTGCTGATGTATTAGA
AACAGCTCCAATCAAAGAAATTATCGTTACAGATTCTGTTAAAACTAAAAATCGTGTGCCAGAAAACGTTACTTATCTTA
GCGCCAGTGATTTAATCGCAGAAGCTATTATTCGTATCCATGAAAGAAGACCCTTAAGTCCGCTTTTTTCTTATCAACCT
AAAGGCAAAAATAACGCATGA

Upstream 100 bases:

>100_bases
TCTGTTAAAATAGGGATGTCAATTAAACACTTTTGGGTAATGTGACTCATTAATAAAACAGTTTACCCAATTTTATTGAA
GTTAAGGAGCTAAATCTATC

Downstream 100 bases:

>100_bases
CTTACTTTGATAACGCCGCCACCACACCACTTAGTCCTAATGTGATTAGGGCGATGACAGCAGCTATGCAAGATAACTTT
GGTAACCCCTCTAGTATTCA

Product: ribose-phosphate pyrophosphokinase

Products: NA

Alternate protein names: RPPK 2; Phosphoribosyl pyrophosphate synthase 2; P-Rib-PP synthase 2; PRPP synthase 2

Number of amino acids: Translated: 326; Mature: 325

Protein sequence:

>326_residues
MTERYADKQIKLFSLTSNLPIAEKIAKAAGIPLGKMSSRQFSNGEIMINIEETVRGDDIYIIQSTSFPVNDNLWELLIMI
DACKRASANTVNIVLPYFGYSRQDRVAKPREPITAKLVANMLTKAGIDRVVTLDLHAVQVQGFFDIPVDNLFTVPLFAER
YSKLGLSGSDVVVVSPKNSGIKRARSLAEYLDSPIAIIDYAQDDSEREQGYIIGDVSGKKAILIDDILNTGKTFAEAAKI
LERSGATDTYAVASHGLFAGGAADVLETAPIKEIIVTDSVKTKNRVPENVTYLSASDLIAEAIIRIHERRPLSPLFSYQP
KGKNNA

Sequences:

>Translated_326_residues
MTERYADKQIKLFSLTSNLPIAEKIAKAAGIPLGKMSSRQFSNGEIMINIEETVRGDDIYIIQSTSFPVNDNLWELLIMI
DACKRASANTVNIVLPYFGYSRQDRVAKPREPITAKLVANMLTKAGIDRVVTLDLHAVQVQGFFDIPVDNLFTVPLFAER
YSKLGLSGSDVVVVSPKNSGIKRARSLAEYLDSPIAIIDYAQDDSEREQGYIIGDVSGKKAILIDDILNTGKTFAEAAKI
LERSGATDTYAVASHGLFAGGAADVLETAPIKEIIVTDSVKTKNRVPENVTYLSASDLIAEAIIRIHERRPLSPLFSYQP
KGKNNA
>Mature_325_residues
TERYADKQIKLFSLTSNLPIAEKIAKAAGIPLGKMSSRQFSNGEIMINIEETVRGDDIYIIQSTSFPVNDNLWELLIMID
ACKRASANTVNIVLPYFGYSRQDRVAKPREPITAKLVANMLTKAGIDRVVTLDLHAVQVQGFFDIPVDNLFTVPLFAERY
SKLGLSGSDVVVVSPKNSGIKRARSLAEYLDSPIAIIDYAQDDSEREQGYIIGDVSGKKAILIDDILNTGKTFAEAAKIL
ERSGATDTYAVASHGLFAGGAADVLETAPIKEIIVTDSVKTKNRVPENVTYLSASDLIAEAIIRIHERRPLSPLFSYQPK
GKNNA

Specific function: Utilized by both the de novo and the salvage pathways by which endogenously formed or exogenously added pyrimidine, purine, or pyridine bases are converted to the corresponding ribonucleoside monophosphates. [C]

COG id: COG0462

COG function: function code FE; Phosphoribosylpyrophosphate synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribose-phosphate pyrophosphokinase family

Homologues:

Organism=Homo sapiens, GI4506127, Length=314, Percent_Identity=42.3566878980892, Blast_Score=252, Evalue=3e-67,
Organism=Homo sapiens, GI28557709, Length=319, Percent_Identity=42.0062695924765, Blast_Score=251, Evalue=6e-67,
Organism=Homo sapiens, GI4506129, Length=314, Percent_Identity=41.7197452229299, Blast_Score=251, Evalue=8e-67,
Organism=Homo sapiens, GI84875539, Length=317, Percent_Identity=41.3249211356467, Blast_Score=247, Evalue=8e-66,
Organism=Homo sapiens, GI4506133, Length=343, Percent_Identity=34.9854227405248, Blast_Score=179, Evalue=3e-45,
Organism=Homo sapiens, GI194018537, Length=330, Percent_Identity=34.5454545454545, Blast_Score=167, Evalue=8e-42,
Organism=Homo sapiens, GI310128524, Length=140, Percent_Identity=37.1428571428571, Blast_Score=97, Evalue=1e-20,
Organism=Homo sapiens, GI310115209, Length=140, Percent_Identity=37.1428571428571, Blast_Score=97, Evalue=1e-20,
Organism=Homo sapiens, GI310118259, Length=140, Percent_Identity=37.1428571428571, Blast_Score=97, Evalue=1e-20,
Organism=Homo sapiens, GI310119946, Length=140, Percent_Identity=37.1428571428571, Blast_Score=97, Evalue=1e-20,
Organism=Escherichia coli, GI1787458, Length=314, Percent_Identity=44.2675159235669, Blast_Score=263, Evalue=1e-71,
Organism=Caenorhabditis elegans, GI25149168, Length=314, Percent_Identity=41.7197452229299, Blast_Score=236, Evalue=1e-62,
Organism=Caenorhabditis elegans, GI17554702, Length=314, Percent_Identity=41.7197452229299, Blast_Score=236, Evalue=1e-62,
Organism=Caenorhabditis elegans, GI71989924, Length=314, Percent_Identity=41.7197452229299, Blast_Score=235, Evalue=3e-62,
Organism=Caenorhabditis elegans, GI17554704, Length=312, Percent_Identity=41.3461538461538, Blast_Score=233, Evalue=1e-61,
Organism=Caenorhabditis elegans, GI17570245, Length=348, Percent_Identity=32.7586206896552, Blast_Score=183, Evalue=9e-47,
Organism=Saccharomyces cerevisiae, GI6319403, Length=321, Percent_Identity=40.1869158878505, Blast_Score=228, Evalue=1e-60,
Organism=Saccharomyces cerevisiae, GI6320946, Length=314, Percent_Identity=41.0828025477707, Blast_Score=227, Evalue=2e-60,
Organism=Saccharomyces cerevisiae, GI6321776, Length=315, Percent_Identity=39.3650793650794, Blast_Score=214, Evalue=1e-56,
Organism=Saccharomyces cerevisiae, GI6322667, Length=191, Percent_Identity=39.2670157068063, Blast_Score=145, Evalue=8e-36,
Organism=Saccharomyces cerevisiae, GI6324511, Length=107, Percent_Identity=44.8598130841121, Blast_Score=82, Evalue=2e-16,
Organism=Drosophila melanogaster, GI21355239, Length=315, Percent_Identity=42.8571428571429, Blast_Score=247, Evalue=7e-66,
Organism=Drosophila melanogaster, GI45551540, Length=338, Percent_Identity=39.9408284023669, Blast_Score=234, Evalue=4e-62,
Organism=Drosophila melanogaster, GI24651458, Length=343, Percent_Identity=34.1107871720117, Blast_Score=186, Evalue=1e-47,
Organism=Drosophila melanogaster, GI24651456, Length=343, Percent_Identity=34.1107871720117, Blast_Score=186, Evalue=1e-47,
Organism=Drosophila melanogaster, GI281362873, Length=343, Percent_Identity=34.1107871720117, Blast_Score=186, Evalue=1e-47,
Organism=Drosophila melanogaster, GI24651454, Length=343, Percent_Identity=34.1107871720117, Blast_Score=186, Evalue=1e-47,
Organism=Drosophila melanogaster, GI24651462, Length=190, Percent_Identity=39.4736842105263, Blast_Score=132, Evalue=2e-31,
Organism=Drosophila melanogaster, GI24651464, Length=190, Percent_Identity=39.4736842105263, Blast_Score=132, Evalue=2e-31,
Organism=Drosophila melanogaster, GI45552010, Length=190, Percent_Identity=39.4736842105263, Blast_Score=132, Evalue=2e-31,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): KPRS2_STRP1 (Q99ZR0)

Other databases:

- EMBL:   AE004092
- EMBL:   CP000017
- RefSeq:   NP_269277.1
- RefSeq:   YP_282208.1
- ProteinModelPortal:   Q99ZR0
- SMR:   Q99ZR0
- EnsemblBacteria:   EBSTRT00000000809
- EnsemblBacteria:   EBSTRT00000027412
- GeneID:   3572054
- GeneID:   901241
- GenomeReviews:   AE004092_GR
- GenomeReviews:   CP000017_GR
- KEGG:   spy:SPy_1123
- KEGG:   spz:M5005_Spy_0845
- GeneTree:   EBGT00050000027352
- HOGENOM:   HBG519284
- OMA:   ELNTIFL
- ProtClustDB:   PRK02458
- BioCyc:   SPYO160490:SPY1123-MONOMER
- BioCyc:   SPYO293653:M5005_SPY0845-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00583_B
- InterPro:   IPR000842
- InterPro:   IPR005946
- InterPro:   IPR000836
- TIGRFAMs:   TIGR01251

Pfam domain/function: PF00156 Pribosyltran

EC number: =2.7.6.1

Molecular weight: Translated: 35708; Mature: 35577

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: PS00114 PRPP_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTERYADKQIKLFSLTSNLPIAEKIAKAAGIPLGKMSSRQFSNGEIMINIEETVRGDDIY
CCCCCCCCEEEEEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEHHHCCCCEEE
IIQSTSFPVNDNLWELLIMIDACKRASANTVNIVLPYFGYSRQDRVAKPREPITAKLVAN
EEECCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHHH
MLTKAGIDRVVTLDLHAVQVQGFFDIPVDNLFTVPLFAERYSKLGLSGSDVVVVSPKNSG
HHHHCCCCEEEEEEEEEEEECCEEECCCCCEEECHHHHHHHHHCCCCCCCEEEECCCCCH
IKRARSLAEYLDSPIAIIDYAQDDSEREQGYIIGDVSGKKAILIDDILNTGKTFAEAAKI
HHHHHHHHHHHCCCEEEEEECCCCCCCCCCEEEEECCCCEEEEEHHHHHCCHHHHHHHHH
LERSGATDTYAVASHGLFAGGAADVLETAPIKEIIVTDSVKTKNRVPENVTYLSASDLIA
HHHCCCCCEEHHHCCCEECCCHHHHHHHCCCHHEEEECCCHHHCCCCCCEEEECHHHHHH
EAIIRIHERRPLSPLFSYQPKGKNNA
HHHHHHHCCCCCCHHHCCCCCCCCCC
>Mature Secondary Structure 
TERYADKQIKLFSLTSNLPIAEKIAKAAGIPLGKMSSRQFSNGEIMINIEETVRGDDIY
CCCCCCCEEEEEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEHHHCCCCEEE
IIQSTSFPVNDNLWELLIMIDACKRASANTVNIVLPYFGYSRQDRVAKPREPITAKLVAN
EEECCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHHH
MLTKAGIDRVVTLDLHAVQVQGFFDIPVDNLFTVPLFAERYSKLGLSGSDVVVVSPKNSG
HHHHCCCCEEEEEEEEEEEECCEEECCCCCEEECHHHHHHHHHCCCCCCCEEEECCCCCH
IKRARSLAEYLDSPIAIIDYAQDDSEREQGYIIGDVSGKKAILIDDILNTGKTFAEAAKI
HHHHHHHHHHHCCCEEEEEECCCCCCCCCCEEEEECCCCEEEEEHHHHHCCHHHHHHHHH
LERSGATDTYAVASHGLFAGGAADVLETAPIKEIIVTDSVKTKNRVPENVTYLSASDLIA
HHHCCCCCEEHHHCCCEECCCHHHHHHHCCCHHEEEECCCHHHCCCCCCEEEECHHHHHH
EAIIRIHERRPLSPLFSYQPKGKNNA
HHHHHHHCCCCCCHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11296296