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The map label for this gene is folE

Identifier: 71910634

GI number: 71910634

Start: 816434

End: 817000

Strand: Direct

Name: folE

Synonym: M5005_Spy_0821

Alternate gene names: 71910634

Gene position: 816434-817000 (Clockwise)

Preceding gene: 71910633

Following gene: 71910635

Centisome position: 44.41

GC content: 41.09

Gene sequence:

>567_bases
ATGTCTATTAATAAGGAAAAAGCAGAAGCAGCCATTTATCAGTTTTTAGAGGCTATTGGTGAAAATCCAAATCGGGAAGG
TCTTCTTGACACGCCTAAACGGGTTGCTAAAATGTATGCGGAGATGTTTTTGGGACTGGGAAAAGATCCTAAAGAGGAGT
TTACGGCTGTCTTTAAAGAACAACATGAGGATGTGGTGATTGTCAAAGACATTAGTTTTTATTCTATATGTGAACACCAC
TTAGTTCCTTTTTATGGCAAGGCTCATATCGCTTACTTGCCAAGTGATGGTCGAGTAACAGGTTTGAGTAAATTAGCGCG
AGCAGTTGAAGTAGCTAGTAAACGACCTCAACTCCAAGAGCGTTTGACCTCTCAAATAGCGGATGCTTTAGTTGAAGCCC
TCAATCCTAAAGGGACTTTAGTTATGGTAGAAGCAGAGCACATGTGCATGACCATGCGAGGCATCAAAAAGCCAGGTAGT
AAAACCATTACCACCACTGCCAGAGGCTTATATAAGGAAAGTCGTGCTGAGAGGCAAGAAGTTATTTCTCTAATGACAAA
AGATTAG

Upstream 100 bases:

>100_bases
ATTAGACAGTATTGGGAAAAACATATTGAAAAAAGCGTTCTTTTAACCCATTAAAAAAGAGAAAATGAAAAGGAAAAAAC
TAATGAAAAGGGAAAGACTA

Downstream 100 bases:

>100_bases
GAGAACTTATGAAAATTGGAAAGTTTGTGATTGAGGGCAATGCGGCTATCATGGGGATTTTAAATGTGACTCCAGATTCT
TTTTCAGATGGGGGGTCTTA

Product: GTP cyclohydrolase I

Products: NA

Alternate protein names: GTP cyclohydrolase I; GTP-CH-I

Number of amino acids: Translated: 188; Mature: 187

Protein sequence:

>188_residues
MSINKEKAEAAIYQFLEAIGENPNREGLLDTPKRVAKMYAEMFLGLGKDPKEEFTAVFKEQHEDVVIVKDISFYSICEHH
LVPFYGKAHIAYLPSDGRVTGLSKLARAVEVASKRPQLQERLTSQIADALVEALNPKGTLVMVEAEHMCMTMRGIKKPGS
KTITTTARGLYKESRAERQEVISLMTKD

Sequences:

>Translated_188_residues
MSINKEKAEAAIYQFLEAIGENPNREGLLDTPKRVAKMYAEMFLGLGKDPKEEFTAVFKEQHEDVVIVKDISFYSICEHH
LVPFYGKAHIAYLPSDGRVTGLSKLARAVEVASKRPQLQERLTSQIADALVEALNPKGTLVMVEAEHMCMTMRGIKKPGS
KTITTTARGLYKESRAERQEVISLMTKD
>Mature_187_residues
SINKEKAEAAIYQFLEAIGENPNREGLLDTPKRVAKMYAEMFLGLGKDPKEEFTAVFKEQHEDVVIVKDISFYSICEHHL
VPFYGKAHIAYLPSDGRVTGLSKLARAVEVASKRPQLQERLTSQIADALVEALNPKGTLVMVEAEHMCMTMRGIKKPGSK
TITTTARGLYKESRAERQEVISLMTKD

Specific function: Tetrahydrofolate biosynthesis; first step. [C]

COG id: COG0302

COG function: function code H; GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family

Homologues:

Organism=Homo sapiens, GI66932968, Length=190, Percent_Identity=50, Blast_Score=180, Evalue=6e-46,
Organism=Homo sapiens, GI4503949, Length=190, Percent_Identity=50, Blast_Score=180, Evalue=6e-46,
Organism=Homo sapiens, GI66932970, Length=150, Percent_Identity=51.3333333333333, Blast_Score=138, Evalue=2e-33,
Organism=Homo sapiens, GI66932972, Length=150, Percent_Identity=51.3333333333333, Blast_Score=138, Evalue=2e-33,
Organism=Escherichia coli, GI1788476, Length=159, Percent_Identity=42.7672955974843, Blast_Score=114, Evalue=6e-27,
Organism=Caenorhabditis elegans, GI17560486, Length=183, Percent_Identity=47.5409836065574, Blast_Score=175, Evalue=1e-44,
Organism=Saccharomyces cerevisiae, GI6321706, Length=184, Percent_Identity=47.2826086956522, Blast_Score=171, Evalue=8e-44,
Organism=Drosophila melanogaster, GI24656782, Length=175, Percent_Identity=49.7142857142857, Blast_Score=168, Evalue=2e-42,
Organism=Drosophila melanogaster, GI24656796, Length=175, Percent_Identity=49.7142857142857, Blast_Score=167, Evalue=4e-42,
Organism=Drosophila melanogaster, GI24656791, Length=175, Percent_Identity=49.7142857142857, Blast_Score=167, Evalue=5e-42,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): GCH1_STRP1 (P0C0C2)

Other databases:

- EMBL:   AE004092
- EMBL:   CP000017
- RefSeq:   NP_269254.1
- RefSeq:   YP_282184.1
- ProteinModelPortal:   P0C0C2
- SMR:   P0C0C2
- EnsemblBacteria:   EBSTRT00000001508
- EnsemblBacteria:   EBSTRT00000028151
- GeneID:   3572069
- GeneID:   901218
- GenomeReviews:   AE004092_GR
- GenomeReviews:   CP000017_GR
- KEGG:   spy:SPy_1097
- KEGG:   spz:M5005_Spy_0821
- GeneTree:   EBGT00050000028325
- HOGENOM:   HBG370191
- OMA:   NHEEVVI
- ProtClustDB:   PRK09347
- BioCyc:   SPYO160490:SPY1097-MONOMER
- BioCyc:   SPYO293653:M5005_SPY0821-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00223
- InterPro:   IPR001474
- InterPro:   IPR020602
- InterPro:   IPR018234
- TIGRFAMs:   TIGR00063

Pfam domain/function: PF01227 GTP_cyclohydroI

EC number: =3.5.4.16

Molecular weight: Translated: 21058; Mature: 20926

Theoretical pI: Translated: 8.22; Mature: 8.22

Prosite motif: PS00859 GTP_CYCLOHYDROL_1_1; PS00860 GTP_CYCLOHYDROL_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSINKEKAEAAIYQFLEAIGENPNREGLLDTPKRVAKMYAEMFLGLGKDPKEEFTAVFKE
CCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
QHEDVVIVKDISFYSICEHHLVPFYGKAHIAYLPSDGRVTGLSKLARAVEVASKRPQLQE
CCCCEEEEECCHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCHHHHH
RLTSQIADALVEALNPKGTLVMVEAEHMCMTMRGIKKPGSKTITTTARGLYKESRAERQE
HHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHH
VISLMTKD
HHHHHCCC
>Mature Secondary Structure 
SINKEKAEAAIYQFLEAIGENPNREGLLDTPKRVAKMYAEMFLGLGKDPKEEFTAVFKE
CCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
QHEDVVIVKDISFYSICEHHLVPFYGKAHIAYLPSDGRVTGLSKLARAVEVASKRPQLQE
CCCCEEEEECCHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCHHHHH
RLTSQIADALVEALNPKGTLVMVEAEHMCMTMRGIKKPGSKTITTTARGLYKESRAERQE
HHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHH
VISLMTKD
HHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11296296