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The map label for this gene is dacA1 [H]

Identifier: 71910630

GI number: 71910630

Start: 812371

End: 813699

Strand: Direct

Name: dacA1 [H]

Synonym: M5005_Spy_0817

Alternate gene names: 71910630

Gene position: 812371-813699 (Clockwise)

Preceding gene: 71910629

Following gene: 71910632

Centisome position: 44.19

GC content: 39.58

Gene sequence:

>1329_bases
ATGAGCTTGTTTAAGCTACTAAAGGTGTGTGCCACTATACTTCCTTCCAAATTTCTGAGAATTTTTGTTATAATAGTAAC
TATGAAAGATATAATGATAAAAAGGTGGGTTATCCTTTTTTTTATGGTATTAACTTGTTCAGGGTTTGGTAAAACAGTTC
TCGCAGCTGATTTTCAAGTGGGAGCAGAACATGCGATTGTCGTAGAGGCAGACTCTGGGAGAGTTCTGTATGAGAAAGAC
GCTAAGACGCCAGATGCTATTGCCTCTTTGACCAAATTGGTGACGGCTTATCTGGTTTTAGATAAGGTTAAATCAGGCCA
GCTGCAATTATCAGATCAAGTTAATCTCTCAGATTATGCCTTCGAGTTAACAAAAGATAGGTCTTTAAGTAATGTACCTT
TTGACAAAAAAACTTATTCAGTTCAAGACCTGTTGACAGCTACTTTGGTAGCAAGCTCAAATAGCGCTGCTATTGCGTTA
GCCGAAAAAGTGGCGGGCTCAGAGCTTCATTTTGTAAATCAAATGAGAGAACAGCTATCTCATTGGGGGATTACTTCTGG
TAAAATCCTCAACGCTTCAGGACTGCCGAATGAGGTGCTAAAGGACCATCGTTATCCTGGTTCTGCGTTGGAAGAAGAGA
ATATGTTGAGTGCTCAGGATGTTGCCATTGTGACGATGCATTTACTGGAAGATTTTCCTGAGATTTTAGAGATCACTAAA
CAAACAGAAGTTGATTTTGCTGGTAATTCTATTAAAAGTTTTAATCAACTCTTACCAGGTATGGCTAAGGGTAGAGCAGG
AGTGGATGGGCTAAAGACAGGAACGACAGATTTGGCAGGTCATTGCCTTGTGGTAACTTCTATTGAAAATGGTATGAGGC
TGATTACCGTTATTCTAAATGCTGATGGATCTGATAAAAACCAAAACACGCGGTTTGAGCAAGCAAATCGCTTGTTGGAC
TATGTGGCTAGAACTTATTGTCGTCGTAAGATTCTTAAAAAAGGAAGTCTTGTCTCGGAGCGATCGCTCCCTATTCAAGA
TGGCCAAGTAAAAGAACTACCGATATCTGTTGCCGAAGATGTAACCATCATTTTGCAACAGGGTGAACAAGTTCCCAAAC
CTAAACAATTTATGATATCAGAGACTTCTTTATTAGCACCGATTACTAAAGGTGAAGTGGTTGCTTATTTGACGTCTCCA
AGAATAACAGATCAGTCAGTGAGGTATTTAAAGGAACCTAAGCGTATTCCTCTTAAAGCTAGCCAATCTCTAAAAAAAGC
CTCTGACTTACAGCTGTGGTGGCGAGATTTTCTTGAAAAAAGACGTTAA

Upstream 100 bases:

>100_bases
ATTTTATACCAAAATTCCTGCCAAATAACTAATTGATATAACGAAGCTTATTATATCGCCCATATAACAGGATGTGTTAT
TGTTACAGGGTACTTGCTTT

Downstream 100 bases:

>100_bases
AGATAAGAATAAAATAATTTTAAAACAATCAAAAAAAGACAGTTATGAAAGAATTTTTTCATAACTGTCTTTTTAGTTTA
CAGTGAGTATGATTTGGAAT

Product: D-alanyl-D-alanine carboxypeptidase

Products: NA

Alternate protein names: CPase; DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 5; PBP-5 [H]

Number of amino acids: Translated: 442; Mature: 441

Protein sequence:

>442_residues
MSLFKLLKVCATILPSKFLRIFVIIVTMKDIMIKRWVILFFMVLTCSGFGKTVLAADFQVGAEHAIVVEADSGRVLYEKD
AKTPDAIASLTKLVTAYLVLDKVKSGQLQLSDQVNLSDYAFELTKDRSLSNVPFDKKTYSVQDLLTATLVASSNSAAIAL
AEKVAGSELHFVNQMREQLSHWGITSGKILNASGLPNEVLKDHRYPGSALEEENMLSAQDVAIVTMHLLEDFPEILEITK
QTEVDFAGNSIKSFNQLLPGMAKGRAGVDGLKTGTTDLAGHCLVVTSIENGMRLITVILNADGSDKNQNTRFEQANRLLD
YVARTYCRRKILKKGSLVSERSLPIQDGQVKELPISVAEDVTIILQQGEQVPKPKQFMISETSLLAPITKGEVVAYLTSP
RITDQSVRYLKEPKRIPLKASQSLKKASDLQLWWRDFLEKRR

Sequences:

>Translated_442_residues
MSLFKLLKVCATILPSKFLRIFVIIVTMKDIMIKRWVILFFMVLTCSGFGKTVLAADFQVGAEHAIVVEADSGRVLYEKD
AKTPDAIASLTKLVTAYLVLDKVKSGQLQLSDQVNLSDYAFELTKDRSLSNVPFDKKTYSVQDLLTATLVASSNSAAIAL
AEKVAGSELHFVNQMREQLSHWGITSGKILNASGLPNEVLKDHRYPGSALEEENMLSAQDVAIVTMHLLEDFPEILEITK
QTEVDFAGNSIKSFNQLLPGMAKGRAGVDGLKTGTTDLAGHCLVVTSIENGMRLITVILNADGSDKNQNTRFEQANRLLD
YVARTYCRRKILKKGSLVSERSLPIQDGQVKELPISVAEDVTIILQQGEQVPKPKQFMISETSLLAPITKGEVVAYLTSP
RITDQSVRYLKEPKRIPLKASQSLKKASDLQLWWRDFLEKRR
>Mature_441_residues
SLFKLLKVCATILPSKFLRIFVIIVTMKDIMIKRWVILFFMVLTCSGFGKTVLAADFQVGAEHAIVVEADSGRVLYEKDA
KTPDAIASLTKLVTAYLVLDKVKSGQLQLSDQVNLSDYAFELTKDRSLSNVPFDKKTYSVQDLLTATLVASSNSAAIALA
EKVAGSELHFVNQMREQLSHWGITSGKILNASGLPNEVLKDHRYPGSALEEENMLSAQDVAIVTMHLLEDFPEILEITKQ
TEVDFAGNSIKSFNQLLPGMAKGRAGVDGLKTGTTDLAGHCLVVTSIENGMRLITVILNADGSDKNQNTRFEQANRLLDY
VARTYCRRKILKKGSLVSERSLPIQDGQVKELPISVAEDVTIILQQGEQVPKPKQFMISETSLLAPITKGEVVAYLTSPR
ITDQSVRYLKEPKRIPLKASQSLKKASDLQLWWRDFLEKRR

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Secreted, cell wall [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1787062, Length=365, Percent_Identity=30.958904109589, Blast_Score=121, Evalue=7e-29,
Organism=Escherichia coli, GI1786851, Length=349, Percent_Identity=27.7936962750716, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI87082022, Length=381, Percent_Identity=26.509186351706, Blast_Score=91, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 49062; Mature: 48930

Theoretical pI: Translated: 9.01; Mature: 9.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLFKLLKVCATILPSKFLRIFVIIVTMKDIMIKRWVILFFMVLTCSGFGKTVLAADFQV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCC
GAEHAIVVEADSGRVLYEKDAKTPDAIASLTKLVTAYLVLDKVKSGQLQLSDQVNLSDYA
CCCEEEEEECCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCHHHHH
FELTKDRSLSNVPFDKKTYSVQDLLTATLVASSNSAAIALAEKVAGSELHFVNQMREQLS
EEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEHHHHCCCHHHHHHHHHHHHH
HWGITSGKILNASGLPNEVLKDHRYPGSALEEENMLSAQDVAIVTMHLLEDFPEILEITK
HCCCCCCCEEECCCCCHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
QTEVDFAGNSIKSFNQLLPGMAKGRAGVDGLKTGTTDLAGHCLVVTSIENGMRLITVILN
HCCCCCCCCHHHHHHHHCCCHHCCCCCCCCCCCCCHHHCCCEEEEEECCCCCEEEEEEEE
ADGSDKNQNTRFEQANRLLDYVARTYCRRKILKKGSLVSERSLPIQDGQVKELPISVAED
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHH
VTIILQQGEQVPKPKQFMISETSLLAPITKGEVVAYLTSPRITDQSVRYLKEPKRIPLKA
HHHEECCCCCCCCCHHHEECCHHHCCCCCCCCEEEEECCCCCCHHHHHHHHCCCCCCCHH
SQSLKKASDLQLWWRDFLEKRR
HHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SLFKLLKVCATILPSKFLRIFVIIVTMKDIMIKRWVILFFMVLTCSGFGKTVLAADFQV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCC
GAEHAIVVEADSGRVLYEKDAKTPDAIASLTKLVTAYLVLDKVKSGQLQLSDQVNLSDYA
CCCEEEEEECCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCHHHHH
FELTKDRSLSNVPFDKKTYSVQDLLTATLVASSNSAAIALAEKVAGSELHFVNQMREQLS
EEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEHHHHCCCHHHHHHHHHHHHH
HWGITSGKILNASGLPNEVLKDHRYPGSALEEENMLSAQDVAIVTMHLLEDFPEILEITK
HCCCCCCCEEECCCCCHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
QTEVDFAGNSIKSFNQLLPGMAKGRAGVDGLKTGTTDLAGHCLVVTSIENGMRLITVILN
HCCCCCCCCHHHHHHHHCCCHHCCCCCCCCCCCCCHHHCCCEEEEEECCCCCEEEEEEEE
ADGSDKNQNTRFEQANRLLDYVARTYCRRKILKKGSLVSERSLPIQDGQVKELPISVAED
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHH
VTIILQQGEQVPKPKQFMISETSLLAPITKGEVVAYLTSPRITDQSVRYLKEPKRIPLKA
HHHEECCCCCCCCCHHHEECCHHHCCCCCCCCEEEEECCCCCCHHHHHHHHCCCCCCCHH
SQSLKKASDLQLWWRDFLEKRR
HHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8370539; 6780559; 111240 [H]