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The map label for this gene is ptsB [C]

Identifier: 71910594

GI number: 71910594

Start: 785664

End: 786155

Strand: Direct

Name: ptsB [C]

Synonym: M5005_Spy_0781

Alternate gene names: 71910594

Gene position: 785664-786155 (Clockwise)

Preceding gene: 71910593

Following gene: 71910595

Centisome position: 42.73

GC content: 41.26

Gene sequence:

>492_bases
ATGATTACCCAAATTCGTGTTGACGACCGTTTGATTCACGGGCAAGTGGCAGTTGTATGGACCAAAGAGTTAAATGCGCC
GCTTTTAGTAGTGGCTAATGATGAAGCTGCAAAAAATGAAATCACACAAATGACGCTTAAAATGGCTGTGCCAAACGGCA
TGAAACTGCTTATTCGTTCAGTTGAAGACTCGATTAAGCTCTTTAATGATCCACGCGCCAAAGACAAGCGTATCTTTGTG
ATTGTCAATTCTGTTAAAGATGCTTGTGCCATTGCTAAAGAAGTTCCAGACTTAGAAGCTGTTAATGTGGCTAATGTGGG
GCGCTTTGATAAGTCAGACCCAGCAAGCAAAGTCAAAGTGACACCAAGTCTTTTACTTAACCCAGAAGAAATGGCAGCCG
CAAAGGAATTAGTCAGTTTACCAGAACTTGATGTCTTTAACCAAGTCTTACCATCAAATACCAAGGTCCATTTGAGCCAA
TTAGTCAACTAA

Upstream 100 bases:

>100_bases
TGTAACCGTTATCAATGAATCGCAAGTTCAGTTAGTGGATTTGGTCCCTGAGACAAATTCAGAAGATGACTTTTTTGATT
AATATCTAGGAGGAAGACAA

Downstream 100 bases:

>100_bases
AGAGAGGTAAAAAAGTATGTTAGTACCAGCAACAATGGCAGCTTTAGCTGTATTGATTTGTTTTGGGGGAAATTATTTAA
CCGGTCAAAGCATGATGGAA

Product: PTS system mannose/fructose family IIB subunit

Products: NA

Alternate protein names: PTS system EIIB component [H]

Number of amino acids: Translated: 163; Mature: 163

Protein sequence:

>163_residues
MITQIRVDDRLIHGQVAVVWTKELNAPLLVVANDEAAKNEITQMTLKMAVPNGMKLLIRSVEDSIKLFNDPRAKDKRIFV
IVNSVKDACAIAKEVPDLEAVNVANVGRFDKSDPASKVKVTPSLLLNPEEMAAAKELVSLPELDVFNQVLPSNTKVHLSQ
LVN

Sequences:

>Translated_163_residues
MITQIRVDDRLIHGQVAVVWTKELNAPLLVVANDEAAKNEITQMTLKMAVPNGMKLLIRSVEDSIKLFNDPRAKDKRIFV
IVNSVKDACAIAKEVPDLEAVNVANVGRFDKSDPASKVKVTPSLLLNPEEMAAAKELVSLPELDVFNQVLPSNTKVHLSQ
LVN
>Mature_163_residues
MITQIRVDDRLIHGQVAVVWTKELNAPLLVVANDEAAKNEITQMTLKMAVPNGMKLLIRSVEDSIKLFNDPRAKDKRIFV
IVNSVKDACAIAKEVPDLEAVNVANVGRFDKSDPASKVKVTPSLLLNPEEMAAAKELVSLPELDVFNQVLPSNTKVHLSQ
LVN

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane [H]

COG id: COG3444

COG function: function code G; Phosphotransferase system, mannose/fructose/N-acetylgalactosamine-specific component IIB

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIB type-4 domain [H]

Homologues:

Organism=Escherichia coli, GI1788120, Length=101, Percent_Identity=31.6831683168317, Blast_Score=67, Evalue=8e-13,
Organism=Escherichia coli, GI87082217, Length=159, Percent_Identity=29.559748427673, Blast_Score=60, Evalue=5e-11,

Paralogues:

None

Copy number: 220 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004720 [H]

Pfam domain/function: PF03830 PTSIIB_sorb [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 17934; Mature: 17934

Theoretical pI: Translated: 6.54; Mature: 6.54

Prosite motif: PS51101 PTS_EIIB_TYPE_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITQIRVDDRLIHGQVAVVWTKELNAPLLVVANDEAAKNEITQMTLKMAVPNGMKLLIRS
CCEEEECCCEEECCEEEEEEEECCCCCEEEEECCCHHHHHHHHHHHEEECCCCHHHHHHH
VEDSIKLFNDPRAKDKRIFVIVNSVKDACAIAKEVPDLEAVNVANVGRFDKSDPASKVKV
HHHHHHHCCCCCCCCCEEEEEEECCHHHHHHHHHCCCCCEECCCCCCCCCCCCCCCEEEE
TPSLLLNPEEMAAAKELVSLPELDVFNQVLPSNTKVHLSQLVN
CHHHEECHHHHHHHHHHHCCCCHHHHHHHCCCCCEEHHHHHCC
>Mature Secondary Structure
MITQIRVDDRLIHGQVAVVWTKELNAPLLVVANDEAAKNEITQMTLKMAVPNGMKLLIRS
CCEEEECCCEEECCEEEEEEEECCCCCEEEEECCCHHHHHHHHHHHEEECCCCHHHHHHH
VEDSIKLFNDPRAKDKRIFVIVNSVKDACAIAKEVPDLEAVNVANVGRFDKSDPASKVKV
HHHHHHHCCCCCCCCCEEEEEEECCHHHHHHHHHCCCCCEECCCCCCCCCCCCCCCEEEE
TPSLLLNPEEMAAAKELVSLPELDVFNQVLPSNTKVHLSQLVN
CHHHEECHHHHHHHHHHHCCCCHHHHHHHCCCCCEEHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA