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The map label for this gene is ybjI [H]

Identifier: 71910283

GI number: 71910283

Start: 458986

End: 459783

Strand: Direct

Name: ybjI [H]

Synonym: M5005_Spy_0470

Alternate gene names: 71910283

Gene position: 458986-459783 (Clockwise)

Preceding gene: 71910282

Following gene: 71910284

Centisome position: 24.96

GC content: 39.85

Gene sequence:

>798_bases
ATGATAAAACTAATAGCAACAGATATGGATGGTACTTTCCTAGCTGAAGATGGTACTTATAATCAAGAGCAACTAGCTGC
GCTCTTGCCAAAGTTAGCCGAAAAAGGCATTTTATTTGCGGTTTCGAGTGGCCGCTCGCTCTTAGCCATTGATCAGCTCT
TTGAGCCCTTTTTGGATCAGATTGCTGTTATTGCAGAAAATGGTTCCGTGGTTCAATACCGTGGTGAGATTCTATTTGCA
GATATGATGACCAAAGAGCAGTATACCGAAGTTGCAAAAAAGATTTTAGCCAATCCTCACTATGTAGAAACAGGTATGGT
TTTTTCTGGGCAAAAGGCAGCCTATATTTTAAAAGGCGCATCAGAAGAGTACATTCAAAAAACAAAACATTACTATGCTA
ATGTTAAGGTTATTAATGGTTTTGAAGATATGGAGAATGACGCCATTTTTAAAGTTTCTACCAACTTCACAGGCCATACC
GTCTTAGAAGGAAGCGATTGGTTGAATCAAGCCCTCCCTTATGCCACTGCAGTGACCACCGGCTTTGATTCCATTGATAT
TATCTTAAAAGAGGTGAATAAGGGCTTTGGTATGGAGCATCTTTGTCAAGCCTTGGGCATTAAGAAAGCAGAAACGATTG
CTTTTGGGGATAATTTTAATGATTACCAGATGTTGGAGTTTGCTGGTAGGGCTATTGCTACGGAAAATGCAAGACCTGAA
ATCAAAGTGATTTCAGACCAAGTCATTGGCCACTGTAATGACGGAGCAGTACTAACGTATTTAAAAGGACTAGTGTAA

Upstream 100 bases:

>100_bases
TGAACAGAATGCAGAAATGATGGGGCTTATTTAAATCGTTAAAAAGTGCACACCCTAAATGGCGTTTGCACCCCCCTAGA
GTAAAAAAAGGAGACATTGT

Downstream 100 bases:

>100_bases
CTCATGAATATTAGAATTTTGGCGCTTGATTTAGACGGTACCCTCTACAACACTGAAAAAATTGTCACAGATGCCAATAA
AAAAGCCTTAGCAGCGGCTA

Product: HAD superfamily hydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 265; Mature: 265

Protein sequence:

>265_residues
MIKLIATDMDGTFLAEDGTYNQEQLAALLPKLAEKGILFAVSSGRSLLAIDQLFEPFLDQIAVIAENGSVVQYRGEILFA
DMMTKEQYTEVAKKILANPHYVETGMVFSGQKAAYILKGASEEYIQKTKHYYANVKVINGFEDMENDAIFKVSTNFTGHT
VLEGSDWLNQALPYATAVTTGFDSIDIILKEVNKGFGMEHLCQALGIKKAETIAFGDNFNDYQMLEFAGRAIATENARPE
IKVISDQVIGHCNDGAVLTYLKGLV

Sequences:

>Translated_265_residues
MIKLIATDMDGTFLAEDGTYNQEQLAALLPKLAEKGILFAVSSGRSLLAIDQLFEPFLDQIAVIAENGSVVQYRGEILFA
DMMTKEQYTEVAKKILANPHYVETGMVFSGQKAAYILKGASEEYIQKTKHYYANVKVINGFEDMENDAIFKVSTNFTGHT
VLEGSDWLNQALPYATAVTTGFDSIDIILKEVNKGFGMEHLCQALGIKKAETIAFGDNFNDYQMLEFAGRAIATENARPE
IKVISDQVIGHCNDGAVLTYLKGLV
>Mature_265_residues
MIKLIATDMDGTFLAEDGTYNQEQLAALLPKLAEKGILFAVSSGRSLLAIDQLFEPFLDQIAVIAENGSVVQYRGEILFA
DMMTKEQYTEVAKKILANPHYVETGMVFSGQKAAYILKGASEEYIQKTKHYYANVKVINGFEDMENDAIFKVSTNFTGHT
VLEGSDWLNQALPYATAVTTGFDSIDIILKEVNKGFGMEHLCQALGIKKAETIAFGDNFNDYQMLEFAGRAIATENARPE
IKVISDQVIGHCNDGAVLTYLKGLV

Specific function: Catalyzes the dephosphorylation of the artificial chromogenic substrate p-nitrophenyl phosphate (pNPP) and of the natural substrates FMN and beta-glucose 1-phosphate [H]

COG id: COG0561

COG function: function code R; Predicted hydrolases of the HAD superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. Cof family [H]

Homologues:

Organism=Escherichia coli, GI87081790, Length=258, Percent_Identity=32.5581395348837, Blast_Score=133, Evalue=1e-32,
Organism=Escherichia coli, GI1787043, Length=265, Percent_Identity=29.811320754717, Blast_Score=121, Evalue=5e-29,
Organism=Escherichia coli, GI87081741, Length=273, Percent_Identity=26.007326007326, Blast_Score=73, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006379
- InterPro:   IPR000150 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: NA

Molecular weight: Translated: 29203; Mature: 29203

Theoretical pI: Translated: 4.55; Mature: 4.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKLIATDMDGTFLAEDGTYNQEQLAALLPKLAEKGILFAVSSGRSLLAIDQLFEPFLDQ
CEEEEEECCCCEEEECCCCCCHHHHHHHHHHHHHCCEEEEECCCCCEEHHHHHHHHHHHH
IAVIAENGSVVQYRGEILFADMMTKEQYTEVAKKILANPHYVETGMVFSGQKAAYILKGA
HEEEECCCCEEEEECCEEEEHHHHHHHHHHHHHHHHCCCCEEECCEEECCCCEEEEEECC
SEEYIQKTKHYYANVKVINGFEDMENDAIFKVSTNFTGHTVLEGSDWLNQALPYATAVTT
CHHHHHHHHHHEEEEEEEECCCCCCCCEEEEEECCCCCCEEECCCHHHHHHCCHHHHHHC
GFDSIDIILKEVNKGFGMEHLCQALGIKKAETIAFGDNFNDYQMLEFAGRAIATENARPE
CCCHHHHHHHHHHCCCCHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHCCCEECCCCCCC
IKVISDQVIGHCNDGAVLTYLKGLV
EEEECHHHCCCCCCCHHHHHHHHCC
>Mature Secondary Structure
MIKLIATDMDGTFLAEDGTYNQEQLAALLPKLAEKGILFAVSSGRSLLAIDQLFEPFLDQ
CEEEEEECCCCEEEECCCCCCHHHHHHHHHHHHHCCEEEEECCCCCEEHHHHHHHHHHHH
IAVIAENGSVVQYRGEILFADMMTKEQYTEVAKKILANPHYVETGMVFSGQKAAYILKGA
HEEEECCCCEEEEECCEEEEHHHHHHHHHHHHHHHHCCCCEEECCEEECCCCEEEEEECC
SEEYIQKTKHYYANVKVINGFEDMENDAIFKVSTNFTGHTVLEGSDWLNQALPYATAVTT
CHHHHHHHHHHEEEEEEEECCCCCCCCEEEEEECCCCCCEEECCCHHHHHHCCHHHHHHC
GFDSIDIILKEVNKGFGMEHLCQALGIKKAETIAFGDNFNDYQMLEFAGRAIATENARPE
CCCHHHHHHHHHHCCCCHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHCCCEECCCCCCC
IKVISDQVIGHCNDGAVLTYLKGLV
EEEECHHHCCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 10493123 [H]