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The map label for this gene is insF1 [H]

Identifier: 71910280

GI number: 71910280

Start: 457645

End: 458358

Strand: Reverse

Name: insF1 [H]

Synonym: M5005_Spy_0467

Alternate gene names: 71910280

Gene position: 458358-457645 (Counterclockwise)

Preceding gene: 71910281

Following gene: 71910268

Centisome position: 24.93

GC content: 33.89

Gene sequence:

>714_bases
TTGTATCGTGTTTTACGTGTCAATCGCTCCACTTACTATAAATTTCTAAAACATAAGCCCTCAAAAAGAGAATTGGATAA
TCAAATTTATAGAAAACAAATACTTGAGATTTATACCAAAGCAAACAAAAGACTTGGTGTGAAGTCTATCAAAGTCATTC
TTCAAAGAGACTACGACACAAAAATCTCTGAAGGAAGAATTTACCGTCTAATGAAGAATATGGCGCTCCCTAAAATGGCT
ACCGTTAAGCCAAAAACAGCTCTTAAAAGGACTCAAAAAACGTATCCTCAAAACTTACTCAACCAGAAATTTAATCCTGA
CAAACCTAATCAAGTATGGTCTACTGACTTCACCTATATTTCTATTGGATATAAGAAATATGTCTATCTCTGCGCAATAC
TTGATCTCTATTCTAGAAAATGTATTGCTTGGAAACTGAGTCACCGTATGGATGCAAAGTTAGCATGTGACACTCTAGAA
TTAGCTCTTAATAAAAGAAAGATTGAAGGAACACTTCTCTTTCATTCCGACCAAGGGTCACAATTTAAGGCCAGGGAATT
GAGAAAAATAATTGATGACAACACTATCATGCATTCTTTTTCTAAACCTGGATATCCTTATGATAATGCCGTAACTGAAG
CATTTTTCAAGTATTTAAAGCATAGACAAATCAACCAAAAAAAGTATCAAACAGGTTCAATTAGACTGCTTTGA

Upstream 100 bases:

>100_bases
AGAACCTTATCCTAAAAAAAGCAAGTGCCATATTCATGCAAAACTTAAAGTAAGACTCCTCGCTGTTTATCGGTTACGCT
TTGAACACGTCACAACAACC

Downstream 100 bases:

>100_bases
ATACATTGAGAATTTTTATAACAATTACAACCCACATACGGCTAATCTAGGACTAACCCCTAATCAGAAAGAAGAAAATT
AGTTTAACGTAATAAAATAA

Product: transposase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 237; Mature: 237

Protein sequence:

>237_residues
MYRVLRVNRSTYYKFLKHKPSKRELDNQIYRKQILEIYTKANKRLGVKSIKVILQRDYDTKISEGRIYRLMKNMALPKMA
TVKPKTALKRTQKTYPQNLLNQKFNPDKPNQVWSTDFTYISIGYKKYVYLCAILDLYSRKCIAWKLSHRMDAKLACDTLE
LALNKRKIEGTLLFHSDQGSQFKARELRKIIDDNTIMHSFSKPGYPYDNAVTEAFFKYLKHRQINQKKYQTGSIRLL

Sequences:

>Translated_237_residues
MYRVLRVNRSTYYKFLKHKPSKRELDNQIYRKQILEIYTKANKRLGVKSIKVILQRDYDTKISEGRIYRLMKNMALPKMA
TVKPKTALKRTQKTYPQNLLNQKFNPDKPNQVWSTDFTYISIGYKKYVYLCAILDLYSRKCIAWKLSHRMDAKLACDTLE
LALNKRKIEGTLLFHSDQGSQFKARELRKIIDDNTIMHSFSKPGYPYDNAVTEAFFKYLKHRQINQKKYQTGSIRLL
>Mature_237_residues
MYRVLRVNRSTYYKFLKHKPSKRELDNQIYRKQILEIYTKANKRLGVKSIKVILQRDYDTKISEGRIYRLMKNMALPKMA
TVKPKTALKRTQKTYPQNLLNQKFNPDKPNQVWSTDFTYISIGYKKYVYLCAILDLYSRKCIAWKLSHRMDAKLACDTLE
LALNKRKIEGTLLFHSDQGSQFKARELRKIIDDNTIMHSFSKPGYPYDNAVTEAFFKYLKHRQINQKKYQTGSIRLL

Specific function: Involved in the transposition of the insertion sequence IS3 [H]

COG id: COG2801

COG function: function code L; Transposase and inactivated derivatives

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Homo sapiens, GI310129419, Length=218, Percent_Identity=31.1926605504587, Blast_Score=113, Evalue=2e-25,
Organism=Escherichia coli, GI1788405, Length=238, Percent_Identity=30.2521008403361, Blast_Score=117, Evalue=6e-28,
Organism=Escherichia coli, GI1787263, Length=238, Percent_Identity=30.2521008403361, Blast_Score=117, Evalue=6e-28,
Organism=Escherichia coli, GI1786753, Length=238, Percent_Identity=30.2521008403361, Blast_Score=117, Evalue=6e-28,
Organism=Escherichia coli, GI1786570, Length=238, Percent_Identity=30.2521008403361, Blast_Score=117, Evalue=6e-28,
Organism=Escherichia coli, GI1786490, Length=238, Percent_Identity=30.2521008403361, Blast_Score=117, Evalue=6e-28,
Organism=Escherichia coli, GI1789981, Length=222, Percent_Identity=30.6306306306306, Blast_Score=100, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 28195; Mature: 28195

Theoretical pI: Translated: 10.62; Mature: 10.62

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYRVLRVNRSTYYKFLKHKPSKRELDNQIYRKQILEIYTKANKRLGVKSIKVILQRDYDT
CCEEEEECHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCC
KISEGRIYRLMKNMALPKMATVKPKTALKRTQKTYPQNLLNQKFNPDKPNQVWSTDFTYI
CCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEECCCCEEE
SIGYKKYVYLCAILDLYSRKCIAWKLSHRMDAKLACDTLELALNKRKIEGTLLFHSDQGS
EECHHHHHHHHHHHHHHCCHHHEEECCCCCCHHHHHHHHHHHHHHHEEEEEEEEECCCCC
QFKARELRKIIDDNTIMHSFSKPGYPYDNAVTEAFFKYLKHRQINQKKYQTGSIRLL
CHHHHHHHHHHCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEC
>Mature Secondary Structure
MYRVLRVNRSTYYKFLKHKPSKRELDNQIYRKQILEIYTKANKRLGVKSIKVILQRDYDT
CCEEEEECHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCC
KISEGRIYRLMKNMALPKMATVKPKTALKRTQKTYPQNLLNQKFNPDKPNQVWSTDFTYI
CCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEECCCCEEE
SIGYKKYVYLCAILDLYSRKCIAWKLSHRMDAKLACDTLELALNKRKIEGTLLFHSDQGS
EECHHHHHHHHHHHHHHCCHHHEEECCCCCCHHHHHHHHHHHHHHHEEEEEEEEECCCCC
QFKARELRKIIDDNTIMHSFSKPGYPYDNAVTEAFFKYLKHRQINQKKYQTGSIRLL
CHHHHHHHHHHCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2987844; 9278503 [H]