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The map label for this gene is potB [H]

Identifier: 71893894

GI number: 71893894

Start: 702003

End: 702812

Strand: Reverse

Name: potB [H]

Synonym: MHJ_0543

Alternate gene names: 71893894

Gene position: 702812-702003 (Counterclockwise)

Preceding gene: 71893895

Following gene: 71893893

Centisome position: 78.32

GC content: 29.14

Gene sequence:

>810_bases
TTAAATCCGCGCATCAGTCTTGTAATTCCCTATGTAATTTTTGCCCTGATTTTTGTGATAATTCCATTAATTTTACTTTT
TGGAAAATCAGTTAGTCCGCTTGTACATAATAGTCAAAGTTTTGATAATCATTTATTAATAAAGGAAAAAACTACCTGGC
AAATTATTGGCCGTTCAATTTTTGTTGGTCTAATTTCGGCTTTTATTTGTTTAATTTTAGCATTTCCCTATGCTTTTTTT
GTTGCAAATTCAAGATCAAAAATATTCAAAATTTATTCGCTTTCATTGATAGCTAGCCCCTTAATTATTTTCACAATTGC
TAAAGTTTTTGCGCTTAGAAGTTTATTTTTACTACTTTTTGATGAAGGTAGTCTTAATAATAATTCTTTTATGGTCTTAG
GTTTAATTTTTTTGAATTTTCCATTTATGGTACTGCCATTATATACAATTTTTCGCGATATGCCCAAAAATTTACTAGAA
GCCGGGACTGATTTAGGTTATTCAAAATTTTGACTTTTAATAAAAGTTGTTCTTCCTTATAGTTTTCGGGCAATTAGTTC
CGGTTTTGCGCTTGTTTTTTTAATGGCAGCTACCTCAATTGTGGTTTCAGATAAACTTTTACCAAATGGATCACAAAACC
AATTAATTGGCAATTTGATTAATAATTCTGCAAATATTTCCAATCCTTTTGATCTTGCCCGGGTTTCATCGCTTGTTTTG
GTGACCCTGCTAGTTTTTATTGGAATTTATAGTCTAATTCATTTTATTCCTATTATTATAATGAAAATTAAAGGATTTAA
ATATGACTAA

Upstream 100 bases:

>100_bases
GTTTTTCTAAAATGAGATGATGATGCTATTCATTTAATGGAGATGGAAAATGCTGAAATTTAATTTTTTTGAAAATTTTT
GAAAAAATTTCAAAAAAAGT

Downstream 100 bases:

>100_bases
AATTATTGATTTTTTCCAAAAATATGAAATTTTGAAAAAATCATACGTTTGATTTTTAATTATTCTTTTTTATATTCCGA
TTATTGTCGGGGCAGTTTTT

Product: spermidine/putrescine ABC transporter permease

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 269; Mature: 269

Protein sequence:

>269_residues
MNPRISLVIPYVIFALIFVIIPLILLFGKSVSPLVHNSQSFDNHLLIKEKTTWQIIGRSIFVGLISAFICLILAFPYAFF
VANSRSKIFKIYSLSLIASPLIIFTIAKVFALRSLFLLLFDEGSLNNNSFMVLGLIFLNFPFMVLPLYTIFRDMPKNLLE
AGTDLGYSKFWLLIKVVLPYSFRAISSGFALVFLMAATSIVVSDKLLPNGSQNQLIGNLINNSANISNPFDLARVSSLVL
VTLLVFIGIYSLIHFIPIIIMKIKGFKYD

Sequences:

>Translated_269_residues
LNPRISLVIPYVIFALIFVIIPLILLFGKSVSPLVHNSQSFDNHLLIKEKTTWQIIGRSIFVGLISAFICLILAFPYAFF
VANSRSKIFKIYSLSLIASPLIIFTIAKVFALRSLFLLLFDEGSLNNNSFMVLGLIFLNFPFMVLPLYTIFRDMPKNLLE
AGTDLGYSKF*LLIKVVLPYSFRAISSGFALVFLMAATSIVVSDKLLPNGSQNQLIGNLINNSANISNPFDLARVSSLVL
VTLLVFIGIYSLIHFIPIIIMKIKGFKYD
>Mature_269_residues
LNPRISLVIPYVIFALIFVIIPLILLFGKSVSPLVHNSQSFDNHLLIKEKTTWQIIGRSIFVGLISAFICLILAFPYAFF
VANSRSKIFKIYSLSLIASPLIIFTIAKVFALRSLFLLLFDEGSLNNNSFMVLGLIFLNFPFMVLPLYTIFRDMPKNLLE
AGTDLGYSKF*LLIKVVLPYSFRAISSGFALVFLMAATSIVVSDKLLPNGSQNQLIGNLINNSANISNPFDLARVSSLVL
VTLLVFIGIYSLIHFIPIIIMKIKGFKYD

Specific function: Required for the activity of the bacterial transport system of putrescine and spermidine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787369, Length=275, Percent_Identity=28.3636363636364, Blast_Score=87, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 29936; Mature: 29936

Theoretical pI: Translated: 10.30; Mature: 10.30

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
LNPRISLVIPYVIFALIFVIIPLILLFGKSVSPLVHNSQSFDNHLLIKEKTTWQIIGRSI
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCEEEEECCHHHHHHHHHH
FVGLISAFICLILAFPYAFFVANSRSKIFKIYSLSLIASPLIIFTIAKVFALRSLFLLLF
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
DEGSLNNNSFMVLGLIFLNFPFMVLPLYTIFRDMPKNLLEAGTDLGYSKFLLIKVVLPYS
CCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
FRAISSGFALVFLMAATSIVVSDKLLPNGSQNQLIGNLINNSANISNPFDLARVSSLVLV
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
TLLVFIGIYSLIHFIPIIIMKIKGFKYD
HHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8948633 [H]