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The map label for this gene is metK [H]

Identifier: 71893799

GI number: 71893799

Start: 565392

End: 566525

Strand: Reverse

Name: metK [H]

Synonym: MHJ_0448

Alternate gene names: 71893799

Gene position: 566525-565392 (Counterclockwise)

Preceding gene: 71893800

Following gene: 71893798

Centisome position: 63.13

GC content: 35.19

Gene sequence:

>1134_bases
TTAGCAGCGAAAATTTCTGTTGCTGAATCAGTCGGAAAAGGTCATCCTGATAAAATTTGTGATCAAATTAGCGATGCTAT
TCTTGATGAAATTCTTAAACAGGATGAGCACGCACGAGTTGCCGTTGAAACAATGGCATCAAACCGCCTAATTATAATTG
GGGGAGAAATCTCAACAAGTGGTTATGTCGATCTTGTAAAAATTGCTTGAGAGATTTTATTTGAAATTGGTTATAACGAA
AATGATTTTACAATAATTTCCAATGTAAATCGCCAATCCGGCGAGATAGCTTCCCTTGTCGATCAGAAAAACGGTACAAT
CGGTGCTGGCGATCAAGGGATTGTTTATGGTTTTGCCACTAACGAGTGCCCAAATTTTATGCCCTTAGCACAAAATTTGG
CTAACTTTTTAGTTAAAAAAGCTGAAGAACTCCGGAAAATAGGTGAATTTCAATTTGCCTGTGCGGATATGAAATCCCAA
GTTGAGATTCACTATGATCAAAACAGTAAACCAAAAGTTAAAAGTATGCTTATGTCAATCCAACATCAGCAGAATTTTAA
CTATCATAATTTTGTTGAATTTGTAATTAACAAAATTATGATTCCAGCAGCAAAAAAATATAATTTAAATATTGACTTTG
AAAAAAAAGTTAACAAATCCGGTGATTTTACAATTGGAGGACCAATTGGTGATACAGGGCTTACGGGGCGAAAACTCATA
ATTGATACTTATGGGGATGCTGCAAAACATGGAGGGGGCGCCTTTAGTGGAAAAGATTATACAAAAGTTGACCGAAGTGG
CGCTTATATTGCCCGGTGAATAGCAAAAAATCTTGTCGCCGCCGGAGTAGCTGCCGAAATCGAGCTACAATTTGCTTATG
CAATCGGTAATGAATATCCAGAACTAATTAATGTTACTTACCTAAAAAATGCTAAAATTCCAACGGTAAAAATTATTGAA
ATTATTAAAAACGTATTTAATCTTCGTCTAGTTAGTTTAATTTCTCGGCTTAATTTGAGGAAACCAATCTACCGAAATTT
AGCAGTTTATGGTCATTTTGGTCGTGATGAGCTCGATCTTCCCTGAGAAAAGCTTGATATGGTTGAAAAAATTAAGGATT
ATCTAAAAATTTAA

Upstream 100 bases:

>100_bases
ATTCCTAATAAAACCCTGCGCAAAAATTTTTTCTAAAAAATTTTTTCATTTTTTTAAAAAAAATGGTATAATATAATGAA
AATTTGAAGGGAAGTAAATG

Downstream 100 bases:

>100_bases
AATTAAAACTTTTTCTAAATGATTAACTACTTTAAAAAAAGCATTTTTTTATTTGGAAAAAATTAAAAAAATGTTATAAT
GTTAATATTATTTTGCCATC

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase [H]

Number of amino acids: Translated: 377; Mature: 377

Protein sequence:

>377_residues
MAAKISVAESVGKGHPDKICDQISDAILDEILKQDEHARVAVETMASNRLIIIGGEISTSGYVDLVKIAWEILFEIGYNE
NDFTIISNVNRQSGEIASLVDQKNGTIGAGDQGIVYGFATNECPNFMPLAQNLANFLVKKAEELRKIGEFQFACADMKSQ
VEIHYDQNSKPKVKSMLMSIQHQQNFNYHNFVEFVINKIMIPAAKKYNLNIDFEKKVNKSGDFTIGGPIGDTGLTGRKLI
IDTYGDAAKHGGGAFSGKDYTKVDRSGAYIARWIAKNLVAAGVAAEIELQFAYAIGNEYPELINVTYLKNAKIPTVKIIE
IIKNVFNLRLVSLISRLNLRKPIYRNLAVYGHFGRDELDLPWEKLDMVEKIKDYLKI

Sequences:

>Translated_377_residues
LAAKISVAESVGKGHPDKICDQISDAILDEILKQDEHARVAVETMASNRLIIIGGEISTSGYVDLVKIA*EILFEIGYNE
NDFTIISNVNRQSGEIASLVDQKNGTIGAGDQGIVYGFATNECPNFMPLAQNLANFLVKKAEELRKIGEFQFACADMKSQ
VEIHYDQNSKPKVKSMLMSIQHQQNFNYHNFVEFVINKIMIPAAKKYNLNIDFEKKVNKSGDFTIGGPIGDTGLTGRKLI
IDTYGDAAKHGGGAFSGKDYTKVDRSGAYIAR*IAKNLVAAGVAAEIELQFAYAIGNEYPELINVTYLKNAKIPTVKIIE
IIKNVFNLRLVSLISRLNLRKPIYRNLAVYGHFGRDELDLP*EKLDMVEKIKDYLKI
>Mature_377_residues
LAAKISVAESVGKGHPDKICDQISDAILDEILKQDEHARVAVETMASNRLIIIGGEISTSGYVDLVKIA*EILFEIGYNE
NDFTIISNVNRQSGEIASLVDQKNGTIGAGDQGIVYGFATNECPNFMPLAQNLANFLVKKAEELRKIGEFQFACADMKSQ
VEIHYDQNSKPKVKSMLMSIQHQQNFNYHNFVEFVINKIMIPAAKKYNLNIDFEKKVNKSGDFTIGGPIGDTGLTGRKLI
IDTYGDAAKHGGGAFSGKDYTKVDRSGAYIAR*IAKNLVAAGVAAEIELQFAYAIGNEYPELINVTYLKNAKIPTVKIIE
IIKNVFNLRLVSLISRLNLRKPIYRNLAVYGHFGRDELDLP*EKLDMVEKIKDYLKI

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family [H]

Homologues:

Organism=Homo sapiens, GI5174529, Length=373, Percent_Identity=42.0911528150134, Blast_Score=264, Evalue=1e-70,
Organism=Homo sapiens, GI4557737, Length=372, Percent_Identity=39.5161290322581, Blast_Score=254, Evalue=9e-68,
Organism=Escherichia coli, GI1789311, Length=380, Percent_Identity=42.6315789473684, Blast_Score=305, Evalue=3e-84,
Organism=Caenorhabditis elegans, GI17538494, Length=367, Percent_Identity=41.41689373297, Blast_Score=246, Evalue=2e-65,
Organism=Caenorhabditis elegans, GI17538498, Length=365, Percent_Identity=41.3698630136986, Blast_Score=245, Evalue=2e-65,
Organism=Caenorhabditis elegans, GI25145633, Length=365, Percent_Identity=39.4520547945205, Blast_Score=242, Evalue=3e-64,
Organism=Caenorhabditis elegans, GI17551082, Length=368, Percent_Identity=39.945652173913, Blast_Score=234, Evalue=7e-62,
Organism=Caenorhabditis elegans, GI32565905, Length=359, Percent_Identity=39.2757660167131, Blast_Score=216, Evalue=1e-56,
Organism=Caenorhabditis elegans, GI32565911, Length=326, Percent_Identity=38.0368098159509, Blast_Score=189, Evalue=2e-48,
Organism=Caenorhabditis elegans, GI25145635, Length=322, Percent_Identity=36.6459627329193, Blast_Score=186, Evalue=2e-47,
Organism=Caenorhabditis elegans, GI32565903, Length=294, Percent_Identity=39.4557823129252, Blast_Score=179, Evalue=2e-45,
Organism=Caenorhabditis elegans, GI32565909, Length=75, Percent_Identity=48, Blast_Score=79, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6320710, Length=383, Percent_Identity=41.77545691906, Blast_Score=247, Evalue=2e-66,
Organism=Saccharomyces cerevisiae, GI6323209, Length=372, Percent_Identity=40.8602150537634, Blast_Score=244, Evalue=1e-65,
Organism=Drosophila melanogaster, GI45552159, Length=365, Percent_Identity=44.9315068493151, Blast_Score=253, Evalue=1e-67,
Organism=Drosophila melanogaster, GI24580529, Length=365, Percent_Identity=44.9315068493151, Blast_Score=253, Evalue=1e-67,
Organism=Drosophila melanogaster, GI24580537, Length=365, Percent_Identity=44.9315068493151, Blast_Score=253, Evalue=1e-67,
Organism=Drosophila melanogaster, GI24580533, Length=365, Percent_Identity=44.9315068493151, Blast_Score=253, Evalue=1e-67,
Organism=Drosophila melanogaster, GI24580535, Length=365, Percent_Identity=44.9315068493151, Blast_Score=253, Evalue=1e-67,
Organism=Drosophila melanogaster, GI24580531, Length=365, Percent_Identity=44.9315068493151, Blast_Score=253, Evalue=1e-67,
Organism=Drosophila melanogaster, GI24580527, Length=365, Percent_Identity=43.8356164383562, Blast_Score=250, Evalue=1e-66,
Organism=Drosophila melanogaster, GI24580525, Length=365, Percent_Identity=43.8356164383562, Blast_Score=250, Evalue=1e-66,
Organism=Drosophila melanogaster, GI24580541, Length=355, Percent_Identity=43.0985915492958, Blast_Score=234, Evalue=1e-61,
Organism=Drosophila melanogaster, GI24580539, Length=166, Percent_Identity=35.5421686746988, Blast_Score=91, Evalue=1e-18,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636 [H]

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N [H]

EC number: =2.5.1.6 [H]

Molecular weight: Translated: 41416; Mature: 41416

Theoretical pI: Translated: 7.20; Mature: 7.20

Prosite motif: PS00376 ADOMET_SYNTHETASE_1 ; PS00377 ADOMET_SYNTHETASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
LAAKISVAESVGKGHPDKICDQISDAILDEILKQDEHARVAVETMASNRLIIIGGEISTS
CCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCHHEEHEECCCCCEEEEECCCCCC
GYVDLVKIAEILFEIGYNENDFTIISNVNRQSGEIASLVDQKNGTIGAGDQGIVYGFATN
CCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHCCCCCCCCCCCCEEEEEECC
ECPNFMPLAQNLANFLVKKAEELRKIGEFQFACADMKSQVEIHYDQNSKPKVKSMLMSIQ
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHH
HQQNFNYHNFVEFVINKIMIPAAKKYNLNIDFEKKVNKSGDFTIGGPIGDTGLTGRKLII
HHCCCCHHHHHHHHHHHHHCCCCCEEECCEEHHHHCCCCCCEEECCCCCCCCCCCCEEEE
DTYGDAAKHGGGAFSGKDYTKVDRSGAYIARIAKNLVAAGVAAEIELQFAYAIGNEYPEL
ECCCCHHHCCCCCCCCCCCCHHCCCCHHHHHHHHHHHHHCCEEEEEEEEEEECCCCCHHH
INVTYLKNAKIPTVKIIEIIKNVFNLRLVSLISRLNLRKPIYRNLAVYGHFGRDELDLPE
EEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHEEEECCCCCCCCCHH
KLDMVEKIKDYLKI
HHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11353084 [H]