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The map label for this gene is proS [H]

Identifier: 71893734

GI number: 71893734

Start: 469539

End: 470978

Strand: Reverse

Name: proS [H]

Synonym: MHJ_0381

Alternate gene names: 71893734

Gene position: 470978-469539 (Counterclockwise)

Preceding gene: 71893737

Following gene: 71893733

Centisome position: 52.48

GC content: 30.9

Gene sequence:

>1440_bases
ATGAAAAAACCTGAGAAAATAACATCAAGAACAGCCAATTTTGCAAAATGATATGTAGATGTTATTACTCAGGCAGATTT
AATGAATTATGGCCCAATTAAGGGAACAATTTATTTTAAACCTTTAGGTTATAAAATTTGGGAAAATATCGTAAAAATTG
TTAATGCTTATTTTGTTAAGCAAAAAATTGAAAATGTTTATTTCCCTCTGTTAATTCCACAGGATTTTATTGAAAAAGAA
AAAAAGCACATTGAAGGTTTTGCTCCTGAATTACTAACCATTACCAAAGTTGGGGGTAAAAATTTAGTCGAAAATATTTA
TATAAGACCAACAAGCGAGCTTCTTTTTGCAGATTATTTTAAGGCAGAAATTGCAAAAAATAACATTTTGCCAATTAAAT
TAAACCAGTGAAGTCAAGTTTTACGCTGAGAAAAAACTACTAACCCGTTTTTGCGAAATACAGAATTTTTATGACAAGAA
GGTCACACAATCCATGCAAGTAAGGTCGAAGCTGACCAATTTGCTAAAAAAATAGCCCGATTTTACAAGTATTTTTTAGA
AAATTATTTGGCCATTCCGGTTATTTCAGGCCAAAAAACTGAGCGTGAAAAGTTTGCTGGCGCTGTAAATACTTATACAG
TTGAAGCAATGATGCAAAATTTTCGCGCCCTACAATCTGCTACTGCTCACTTTTTAGGTCAAAATTTTGCAAAAAATTTT
GAAATTTTCTATAAAAATAAAGAAAATAAAAGTCAAATCCCTTTCCAAACCTCTTGAGGACTTTCAACACGCCTAATCGG
GGCAATTGTAATGGTTCATAGCGATGATAACGGACTTATTTTCCCACCTAAAATTGCTCCAATTCAGGTTGATATTCTTG
AATTTTTTTCTAAAAAAAATCAAGAAGTTAAAATTTTTGCAAAGAAAATCGCAAAAATTTTAAAAAACGCTAAAATTTCT
TATAAAATTGATGACACGGATCAACAAATCGGATACAAAATTAATAATTCAGAGGTTCACGGCGCACCAATTCGGATTGA
AATTGGCCCTAATGAGGTCAAAAATAATCAAATTTGCTTAGTTCGACGTGATAACCACCAAAAATTTTTTTTCAATATTG
ATCTGTTAAAAGAAAAATGCCGTAAAATTCTTGAACAAATTCAAGCTGATTTATTCAAAAAAGCAAAAATTCGACTTCTT
GAAAATACAGTTTTTGTAAATTCTATTAATGAATTCGAACAGGCAATAAAAAATAATAAATTTGTAATTGCCCCTTTTAG
TGAATCTCCCGAAAGAGAGCAAGAAATTCAGGAAAAAACCGGAGCTACTGCCCGCTGCATCCTTCCCAAAAATTCATTTT
TTGCCTTACCACAGACAGGAAATTCAATTTTTAGTGGCGAAAAAACAAATAAATTTGTTTTGTTTGCAAAATCTTACTAA

Upstream 100 bases:

>100_bases
AAGGCAACGGTTTTTGGTACCGTCATTCGTTAGTTCGAATCTAACTGGGGCAGCCATATGTTAATTCACCACACCCAAAA
AGTTATAAAAAAACAAGTTT

Downstream 100 bases:

>100_bases
ATTTTTAATTAAATTTTTTTCTAAAAATTCAAAAAATGTAGTAAAATTAAATAGAAAAGGAGAAATATGCCAATTTTTGA
CATCCACTCAACTGAGGATA

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS [H]

Number of amino acids: Translated: 479; Mature: 479

Protein sequence:

>479_residues
MKKPEKITSRTANFAKWYVDVITQADLMNYGPIKGTIYFKPLGYKIWENIVKIVNAYFVKQKIENVYFPLLIPQDFIEKE
KKHIEGFAPELLTITKVGGKNLVENIYIRPTSELLFADYFKAEIAKNNILPIKLNQWSQVLRWEKTTNPFLRNTEFLWQE
GHTIHASKVEADQFAKKIARFYKYFLENYLAIPVISGQKTEREKFAGAVNTYTVEAMMQNFRALQSATAHFLGQNFAKNF
EIFYKNKENKSQIPFQTSWGLSTRLIGAIVMVHSDDNGLIFPPKIAPIQVDILEFFSKKNQEVKIFAKKIAKILKNAKIS
YKIDDTDQQIGYKINNSEVHGAPIRIEIGPNEVKNNQICLVRRDNHQKFFFNIDLLKEKCRKILEQIQADLFKKAKIRLL
ENTVFVNSINEFEQAIKNNKFVIAPFSESPEREQEIQEKTGATARCILPKNSFFALPQTGNSIFSGEKTNKFVLFAKSY

Sequences:

>Translated_479_residues
MKKPEKITSRTANFAK*YVDVITQADLMNYGPIKGTIYFKPLGYKIWENIVKIVNAYFVKQKIENVYFPLLIPQDFIEKE
KKHIEGFAPELLTITKVGGKNLVENIYIRPTSELLFADYFKAEIAKNNILPIKLNQ*SQVLR*EKTTNPFLRNTEFL*QE
GHTIHASKVEADQFAKKIARFYKYFLENYLAIPVISGQKTEREKFAGAVNTYTVEAMMQNFRALQSATAHFLGQNFAKNF
EIFYKNKENKSQIPFQTS*GLSTRLIGAIVMVHSDDNGLIFPPKIAPIQVDILEFFSKKNQEVKIFAKKIAKILKNAKIS
YKIDDTDQQIGYKINNSEVHGAPIRIEIGPNEVKNNQICLVRRDNHQKFFFNIDLLKEKCRKILEQIQADLFKKAKIRLL
ENTVFVNSINEFEQAIKNNKFVIAPFSESPEREQEIQEKTGATARCILPKNSFFALPQTGNSIFSGEKTNKFVLFAKSY
>Mature_479_residues
MKKPEKITSRTANFAK*YVDVITQADLMNYGPIKGTIYFKPLGYKIWENIVKIVNAYFVKQKIENVYFPLLIPQDFIEKE
KKHIEGFAPELLTITKVGGKNLVENIYIRPTSELLFADYFKAEIAKNNILPIKLNQ*SQVLR*EKTTNPFLRNTEFL*QE
GHTIHASKVEADQFAKKIARFYKYFLENYLAIPVISGQKTEREKFAGAVNTYTVEAMMQNFRALQSATAHFLGQNFAKNF
EIFYKNKENKSQIPFQTS*GLSTRLIGAIVMVHSDDNGLIFPPKIAPIQVDILEFFSKKNQEVKIFAKKIAKILKNAKIS
YKIDDTDQQIGYKINNSEVHGAPIRIEIGPNEVKNNQICLVRRDNHQKFFFNIDLLKEKCRKILEQIQADLFKKAKIRLL
ENTVFVNSINEFEQAIKNNKFVIAPFSESPEREQEIQEKTGATARCILPKNSFFALPQTGNSIFSGEKTNKFVLFAKSY

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro) [H]

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 3 subfamily [H]

Homologues:

Organism=Homo sapiens, GI62241042, Length=487, Percent_Identity=36.9609856262834, Blast_Score=286, Evalue=3e-77,
Organism=Caenorhabditis elegans, GI71991342, Length=491, Percent_Identity=34.826883910387, Blast_Score=284, Evalue=6e-77,
Organism=Caenorhabditis elegans, GI71991346, Length=357, Percent_Identity=39.4957983193277, Blast_Score=251, Evalue=8e-67,
Organism=Saccharomyces cerevisiae, GI6321808, Length=436, Percent_Identity=37.8440366972477, Blast_Score=291, Evalue=1e-79,
Organism=Drosophila melanogaster, GI24649468, Length=488, Percent_Identity=33.8114754098361, Blast_Score=259, Evalue=3e-69,
Organism=Drosophila melanogaster, GI24649466, Length=488, Percent_Identity=33.8114754098361, Blast_Score=259, Evalue=4e-69,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004499
- InterPro:   IPR017449
- InterPro:   IPR016061 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF09180 ProRS-C_1; PF00587 tRNA-synt_2b [H]

EC number: =6.1.1.15 [H]

Molecular weight: Translated: 54455; Mature: 54455

Theoretical pI: Translated: 10.06; Mature: 10.06

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKPEKITSRTANFAKYVDVITQADLMNYGPIKGTIYFKPLGYKIWENIVKIVNAYFVKQ
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHH
KIENVYFPLLIPQDFIEKEKKHIEGFAPELLTITKVGGKNLVENIYIRPTSELLFADYFK
HHHHEEEEEECCHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHEEEECCCHHHHHHHHHH
AEIAKNNILPIKLNQSQVLREKTTNPFLRNTEFLQEGHTIHASKVEADQFAKKIARFYKY
HHHHCCCEEEEEECHHHHHHHHCCCCCCCCCHHHHCCCEEEECCCCHHHHHHHHHHHHHH
FLENYLAIPVISGQKTEREKFAGAVNTYTVEAMMQNFRALQSATAHFLGQNFAKNFEIFY
HHHCCEEEEEECCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEE
KNKENKSQIPFQTSGLSTRLIGAIVMVHSDDNGLIFPPKIAPIQVDILEFFSKKNQEVKI
ECCCCCCCCCEECCCCCCEEEEEEEEEEECCCCEEECCCCCCEEHHHHHHHCCCCCHHHH
FAKKIAKILKNAKISYKIDDTDQQIGYKINNSEVHGAPIRIEIGPNEVKNNQICLVRRDN
HHHHHHHHHHCCEEEEEECCCCHHCCEEECCCEECCCEEEEEECCCCCCCCEEEEEEECC
HQKFFFNIDLLKEKCRKILEQIQADLFKKAKIRLLENTVFVNSINEFEQAIKNNKFVIAP
CCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEHHHHHHHHHHCCCCEEEEE
FSESPEREQEIQEKTGATARCILPKNSFFALPQTGNSIFSGEKTNKFVLFAKSY
CCCCCHHHHHHHHHCCCEEEEEECCCCEEECCCCCCCCCCCCCCCEEEEEEECC
>Mature Secondary Structure
MKKPEKITSRTANFAKYVDVITQADLMNYGPIKGTIYFKPLGYKIWENIVKIVNAYFVKQ
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHH
KIENVYFPLLIPQDFIEKEKKHIEGFAPELLTITKVGGKNLVENIYIRPTSELLFADYFK
HHHHEEEEEECCHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHEEEECCCHHHHHHHHHH
AEIAKNNILPIKLNQSQVLREKTTNPFLRNTEFLQEGHTIHASKVEADQFAKKIARFYKY
HHHHCCCEEEEEECHHHHHHHHCCCCCCCCCHHHHCCCEEEECCCCHHHHHHHHHHHHHH
FLENYLAIPVISGQKTEREKFAGAVNTYTVEAMMQNFRALQSATAHFLGQNFAKNFEIFY
HHHCCEEEEEECCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEE
KNKENKSQIPFQTSGLSTRLIGAIVMVHSDDNGLIFPPKIAPIQVDILEFFSKKNQEVKI
ECCCCCCCCCEECCCCCCEEEEEEEEEEECCCCEEECCCCCCEEHHHHHHHCCCCCHHHH
FAKKIAKILKNAKISYKIDDTDQQIGYKINNSEVHGAPIRIEIGPNEVKNNQICLVRRDN
HHHHHHHHHHCCEEEEEECCCCHHCCEEECCCEECCCEEEEEECCCCCCCCEEEEEEECC
HQKFFFNIDLLKEKCRKILEQIQADLFKKAKIRLLENTVFVNSINEFEQAIKNNKFVIAP
CCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEHHHHHHHHHHCCCCEEEEE
FSESPEREQEIQEKTGATARCILPKNSFFALPQTGNSIFSGEKTNKFVLFAKSY
CCCCCHHHHHHHHHCCCEEEEEECCCCEEECCCCCCCCCCCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA