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The map label for this gene is baiH [H]

Identifier: 71893656

GI number: 71893656

Start: 363383

End: 364555

Strand: Reverse

Name: baiH [H]

Synonym: MHJ_0301

Alternate gene names: 71893656

Gene position: 364555-363383 (Counterclockwise)

Preceding gene: 71893657

Following gene: 71893654

Centisome position: 40.62

GC content: 30.78

Gene sequence:

>1173_bases
ATGAAAAATAAACTTTTTAAACCGTATCAAATTGGTAATTTCACATTAAAAAACCGATTTGTTTTTAGCCCAATTAATCT
TAATCTTTCTGAAATGGGCCAAGTTTGCCAAGCAGATTTAGATTTTTTTAGCGCTAGAGCCCATAGTGCACCAATGACAA
TTACAGGGGGAGTTTATATTTGTAAATCAGGTAGTTTGTTCAAATATGGTCATAGTATCGAAAATGATTGCGATATTGAA
GGTCTAGCAAAACTTGCAAAGGCAATAAAATCAAAAGGAAGTATCGCAATTTTGCAATTAATTCATGCTGGTTCTCACTC
TGTTTCAAAAATTGAAAAAGATGGTTTTTGCTATGGACCTAGTGCTGAAAAGCGAAATTATCCTGTAAAATACAAGGTTT
ATCGTCTTAGACAAAAGCAAATTAAAACCATTGTTAGACAATATGCAGATGCTTGTCTAAGGGCAATTAAGGCAGGTTTT
GATGGAGTTGAAATTTCTTCGGCCCAAAAATTATTACCTCAAGAATTTTTCTCAAATTATACTAACAAGAGAAATGATAA
ATATGGCTGTCAAAATATTGAAAATAGATCTCGGTTTTTGCTAGAAATATTAGAAAAAGTACAAAAAACTATTTTAAAAT
CAAAAAATCCTAATTTTTTGCTAGGTTACCGGGCAACACCTGAGGAACGAAGAGGCGATAATTTAGGTTATACAGTCGAA
AATTTCCTAGATTTCTTAGATTTAATTGAAAAAAGGGGGATAAAAATTTCTTACCTTGCAATTGCCGGTTGAGGTTTTGA
CGTTTTTCGTGACAAAGTTCGGTCAAAATCAAATAATTTTAACCGTTTAATTTCTGATGTAGTTTATGAAAAATTGCAAG
GAAAAATCCCTGTTATTGCTAGTGGTTCAATAAATTCCTTAGAAAAATCAGTTAAAGCTATTCAATTTGCTGATTTTATC
GGAATTTCTTCAGCCTTTGTGCCTGATCCTGATTTTGTTGAAAAAATTGAGAAAAATCAGCCAATAAAATTAAGTCTTGA
GCCGGAAGATTTTGCAAAATTAAAAATAAGTAAAAACGCATTTAAAGAAATTGGTCATTATTTTGAATATGGCAAATCTT
TACCTGAAAATGCTCGAAAAACTATTTTAAATAATTCAAAAAAAGCAGAATAA

Upstream 100 bases:

>100_bases
AAGATCAACTATTAAATCGGCTAAAAGATGCAAAATTGGAAGATTATTTTAGTCAAAAAATTGATGAAGAAGAAATTTGT
AACTTACTTTTAAACTAAAT

Downstream 100 bases:

>100_bases
TTTTTTTCTTTGAAAAATCAATAAAAAAATATACAATTATACATTTATTAATTATATATTCCCAAATTCAAAATATAAAA
GCAACACACAGATATTTTTA

Product: NADH-dependent flavin oxidoreductase

Products: trans-transtetradehydroacyl-CoA; NADPH [C]

Alternate protein names: NA

Number of amino acids: Translated: 390; Mature: 390

Protein sequence:

>390_residues
MKNKLFKPYQIGNFTLKNRFVFSPINLNLSEMGQVCQADLDFFSARAHSAPMTITGGVYICKSGSLFKYGHSIENDCDIE
GLAKLAKAIKSKGSIAILQLIHAGSHSVSKIEKDGFCYGPSAEKRNYPVKYKVYRLRQKQIKTIVRQYADACLRAIKAGF
DGVEISSAQKLLPQEFFSNYTNKRNDKYGCQNIENRSRFLLEILEKVQKTILKSKNPNFLLGYRATPEERRGDNLGYTVE
NFLDFLDLIEKRGIKISYLAIAGWGFDVFRDKVRSKSNNFNRLISDVVYEKLQGKIPVIASGSINSLEKSVKAIQFADFI
GISSAFVPDPDFVEKIEKNQPIKLSLEPEDFAKLKISKNAFKEIGHYFEYGKSLPENARKTILNNSKKAE

Sequences:

>Translated_390_residues
MKNKLFKPYQIGNFTLKNRFVFSPINLNLSEMGQVCQADLDFFSARAHSAPMTITGGVYICKSGSLFKYGHSIENDCDIE
GLAKLAKAIKSKGSIAILQLIHAGSHSVSKIEKDGFCYGPSAEKRNYPVKYKVYRLRQKQIKTIVRQYADACLRAIKAGF
DGVEISSAQKLLPQEFFSNYTNKRNDKYGCQNIENRSRFLLEILEKVQKTILKSKNPNFLLGYRATPEERRGDNLGYTVE
NFLDFLDLIEKRGIKISYLAIAG*GFDVFRDKVRSKSNNFNRLISDVVYEKLQGKIPVIASGSINSLEKSVKAIQFADFI
GISSAFVPDPDFVEKIEKNQPIKLSLEPEDFAKLKISKNAFKEIGHYFEYGKSLPENARKTILNNSKKAE
>Mature_390_residues
MKNKLFKPYQIGNFTLKNRFVFSPINLNLSEMGQVCQADLDFFSARAHSAPMTITGGVYICKSGSLFKYGHSIENDCDIE
GLAKLAKAIKSKGSIAILQLIHAGSHSVSKIEKDGFCYGPSAEKRNYPVKYKVYRLRQKQIKTIVRQYADACLRAIKAGF
DGVEISSAQKLLPQEFFSNYTNKRNDKYGCQNIENRSRFLLEILEKVQKTILKSKNPNFLLGYRATPEERRGDNLGYTVE
NFLDFLDLIEKRGIKISYLAIAG*GFDVFRDKVRSKSNNFNRLISDVVYEKLQGKIPVIASGSINSLEKSVKAIQFADFI
GISSAFVPDPDFVEKIEKNQPIKLSLEPEDFAKLKISKNAFKEIGHYFEYGKSLPENARKTILNNSKKAE

Specific function: Catalyzes The NADP-Dependent Reduction Of 2,4-Dienoyl- CoA To Yield Trans-2- Enoyl-CoA. [C]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1789463, Length=341, Percent_Identity=23.4604105571848, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1787939, Length=371, Percent_Identity=23.989218328841, Blast_Score=86, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17559802, Length=340, Percent_Identity=26.7647058823529, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17540738, Length=269, Percent_Identity=29.7397769516729, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17565138, Length=332, Percent_Identity=25.3012048192771, Blast_Score=79, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17559804, Length=283, Percent_Identity=26.8551236749117, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17564188, Length=288, Percent_Identity=24.6527777777778, Blast_Score=75, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI72001454, Length=283, Percent_Identity=26.8551236749117, Blast_Score=74, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17566914, Length=196, Percent_Identity=29.0816326530612, Blast_Score=70, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6321973, Length=371, Percent_Identity=27.2237196765499, Blast_Score=99, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6325086, Length=361, Percent_Identity=26.0387811634349, Blast_Score=97, Evalue=5e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001155 [H]

Pfam domain/function: PF00724 Oxidored_FMN [H]

EC number: 1.3.1.34 [C]

Molecular weight: Translated: 43953; Mature: 43953

Theoretical pI: Translated: 10.00; Mature: 10.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNKLFKPYQIGNFTLKNRFVFSPINLNLSEMGQVCQADLDFFSARAHSAPMTITGGVYI
CCCCCCCCEECCCEEECCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEECCEEE
CKSGSLFKYGHSIENDCDIEGLAKLAKAIKSKGSIAILQLIHAGSHSVSKIEKDGFCYGP
ECCCCEEECCCCCCCCCCHHHHHHHHHHHHCCCCEEEHHHHHCCCHHHHHHHCCCCEECC
SAEKRNYPVKYKVYRLRQKQIKTIVRQYADACLRAIKAGFDGVEISSAQKLLPQEFFSNY
CCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
TNKRNDKYGCQNIENRSRFLLEILEKVQKTILKSKNPNFLLGYRATPEERRGDNLGYTVE
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHCCCCCCCCHH
NFLDFLDLIEKRGIKISYLAIAGGFDVFRDKVRSKSNNFNRLISDVVYEKLQGKIPVIAS
HHHHHHHHHHHCCCEEEEEEEECCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCEEEC
GSINSLEKSVKAIQFADFIGISSAFVPDPDFVEKIEKNQPIKLSLEPEDFAKLKISKNAF
CCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCEEEEECCCCHHHEEECHHHH
KEIGHYFEYGKSLPENARKTILNNSKKAE
HHHHHHHHHCCCCCHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKNKLFKPYQIGNFTLKNRFVFSPINLNLSEMGQVCQADLDFFSARAHSAPMTITGGVYI
CCCCCCCCEECCCEEECCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEECCEEE
CKSGSLFKYGHSIENDCDIEGLAKLAKAIKSKGSIAILQLIHAGSHSVSKIEKDGFCYGP
ECCCCEEECCCCCCCCCCHHHHHHHHHHHHCCCCEEEHHHHHCCCHHHHHHHCCCCEECC
SAEKRNYPVKYKVYRLRQKQIKTIVRQYADACLRAIKAGFDGVEISSAQKLLPQEFFSNY
CCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
TNKRNDKYGCQNIENRSRFLLEILEKVQKTILKSKNPNFLLGYRATPEERRGDNLGYTVE
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHCCCCCCCCHH
NFLDFLDLIEKRGIKISYLAIAGGFDVFRDKVRSKSNNFNRLISDVVYEKLQGKIPVIAS
HHHHHHHHHHHCCCEEEEEEEECCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCEEEC
GSINSLEKSVKAIQFADFIGISSAFVPDPDFVEKIEKNQPIKLSLEPEDFAKLKISKNAF
CCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCEEEEECCCCHHHEEECHHHH
KEIGHYFEYGKSLPENARKTILNNSKKAE
HHHHHHHHHCCCCCHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FAD. [C]

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: transDidehydroacyl-CoA; NADP+ [C]

Specific reaction: transDidehydroacyl-CoA + NADP+ =trans-transtetradehydroacyl-CoA + NADPH [C]

General reaction: Redox reaction [C]

Inhibitor: Iodoacetic acid; N-Ethyl maleimide [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377 [H]