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The map label for this gene is ychF [H]

Identifier: 71893640

GI number: 71893640

Start: 339318

End: 340421

Strand: Reverse

Name: ychF [H]

Synonym: MHJ_0284

Alternate gene names: 71893640

Gene position: 340421-339318 (Counterclockwise)

Preceding gene: 161611220

Following gene: 71893638

Centisome position: 37.93

GC content: 29.89

Gene sequence:

>1104_bases
ATGAATTTAAAAGCAGGTCTCATTGGACTTCCAAATGTCGGAAAATCTTCACTTTTTTCAGCATTAACAAAAATGAATGT
TGAAATTGCAAATTATCCTTTTGCAACAATTGAACCAAATATTGCCACTGTTGAAATTCATGACCCGCGAATTCTTCAAC
TAACAAAAATAGTAAAACCCGAAAAAACCGTTTTTGCTACCTATTCTTTTGTTGATATTGCTGGACTAATTAAAGGGGCT
TCTACAGGCGAAGGTTTGGGTAATAAATTTCTTGCAAATGTAAGAAATGTGGATTGTTTAGTGCATATTGTTCGTTGTTT
TCAGGATCCAAAAATTATTCATGTCAATAACGAAATTAACCCAATTTTTGATATCCAGACTATAAATCTTGAACTAATTT
TTGCAGATCTTGGAACAATTCAGACAATAATTTCCCGACTTGCAAAAAAGGCAAATAATACTAATGATAAACAAGTTAAA
TTTGAATTTGAACTTGCAAAAAAAGTTGAAATTCATTTAAAAAATGGTAAATCACTAAGGGATTTAGAGTTAGATTCTAC
CGAAATTTTACAAATAAAATCTTGACAACTTCTAACGATCAAACCGGTTTTATATGTTGCTAATTTAGATCAAAAATCAA
TCCAGAATCCAGATGCAAATCCTTATTTTTGAAAACTAAAAAGTTATTTAGTCAAAGAAAAAGCAATTTTACTTCCGCTT
TGTATTGCTTTAGAACAGGAAATTTCCCAGCTCGATGAAGAAGAAAAACACTTGTTTTTAAAGGAGTTTTTACTTGAAAA
ATCTTCACTTGATCTTTTAATTCTACATAGTTTTTATCTTCTAGATCTAGCAACTTTTTTTACTGTTGGCAAAAAAGAAG
TAAGATCTTGAACTTTTCGGAAAAACACACCCGCAATTGAATGTAGCGGAATTATTCATTCCGATTTTAAAAAAAAATTT
ATCCGTGTTGAAATTATTAGTTTTGATGATTTTGTTCAAGCTGGTAGCGAAAAAGTGGTTAAAGAAAATGGTAAAATGCG
ACTTGAAGGTAAGGATTATTTAATAAAAGATGGTGAAATTTGCCACTTTCGAATTGGTAATTAA

Upstream 100 bases:

>100_bases
AGCGCTTGCAGTTAAAAGAGCCCGTTTTGTGGCTCTTCTTCCTTTTATCGGTGATCGCATCCGCGGAAATTATGATAAAA
CCCGTGTCTAAATTGAAAAA

Downstream 100 bases:

>100_bases
TTCTAATTTTTCTTATTAACTAGTCAATCTAAAAAAATCATTTCAAGATCATTACAATGTTGTTCTTAAACTAAGAAAAA
TCCAATTTTACTTTTGATTT

Product: GTP-dependent nucleic acid-binding protein EngD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 367; Mature: 367

Protein sequence:

>367_residues
MNLKAGLIGLPNVGKSSLFSALTKMNVEIANYPFATIEPNIATVEIHDPRILQLTKIVKPEKTVFATYSFVDIAGLIKGA
STGEGLGNKFLANVRNVDCLVHIVRCFQDPKIIHVNNEINPIFDIQTINLELIFADLGTIQTIISRLAKKANNTNDKQVK
FEFELAKKVEIHLKNGKSLRDLELDSTEILQIKSWQLLTIKPVLYVANLDQKSIQNPDANPYFWKLKSYLVKEKAILLPL
CIALEQEISQLDEEEKHLFLKEFLLEKSSLDLLILHSFYLLDLATFFTVGKKEVRSWTFRKNTPAIECSGIIHSDFKKKF
IRVEIISFDDFVQAGSEKVVKENGKMRLEGKDYLIKDGEICHFRIGN

Sequences:

>Translated_367_residues
MNLKAGLIGLPNVGKSSLFSALTKMNVEIANYPFATIEPNIATVEIHDPRILQLTKIVKPEKTVFATYSFVDIAGLIKGA
STGEGLGNKFLANVRNVDCLVHIVRCFQDPKIIHVNNEINPIFDIQTINLELIFADLGTIQTIISRLAKKANNTNDKQVK
FEFELAKKVEIHLKNGKSLRDLELDSTEILQIKS*QLLTIKPVLYVANLDQKSIQNPDANPYF*KLKSYLVKEKAILLPL
CIALEQEISQLDEEEKHLFLKEFLLEKSSLDLLILHSFYLLDLATFFTVGKKEVRS*TFRKNTPAIECSGIIHSDFKKKF
IRVEIISFDDFVQAGSEKVVKENGKMRLEGKDYLIKDGEICHFRIGN
>Mature_367_residues
MNLKAGLIGLPNVGKSSLFSALTKMNVEIANYPFATIEPNIATVEIHDPRILQLTKIVKPEKTVFATYSFVDIAGLIKGA
STGEGLGNKFLANVRNVDCLVHIVRCFQDPKIIHVNNEINPIFDIQTINLELIFADLGTIQTIISRLAKKANNTNDKQVK
FEFELAKKVEIHLKNGKSLRDLELDSTEILQIKS*QLLTIKPVLYVANLDQKSIQNPDANPYF*KLKSYLVKEKAILLPL
CIALEQEISQLDEEEKHLFLKEFLLEKSSLDLLILHSFYLLDLATFFTVGKKEVRS*TFRKNTPAIECSGIIHSDFKKKF
IRVEIISFDDFVQAGSEKVVKENGKMRLEGKDYLIKDGEICHFRIGN

Specific function: GTP-dependent nucleic acid-binding protein which may act as a translation factor [H]

COG id: COG0012

COG function: function code J; Predicted GTPase, probable translation factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain [H]

Homologues:

Organism=Homo sapiens, GI58761500, Length=367, Percent_Identity=38.4196185286104, Blast_Score=242, Evalue=4e-64,
Organism=Homo sapiens, GI58761502, Length=229, Percent_Identity=35.3711790393013, Blast_Score=123, Evalue=3e-28,
Organism=Escherichia coli, GI1787454, Length=363, Percent_Identity=46.831955922865, Blast_Score=343, Evalue=9e-96,
Organism=Escherichia coli, GI1789574, Length=99, Percent_Identity=30.3030303030303, Blast_Score=65, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI17509631, Length=364, Percent_Identity=37.0879120879121, Blast_Score=238, Evalue=3e-63,
Organism=Caenorhabditis elegans, GI17552324, Length=139, Percent_Identity=32.3741007194245, Blast_Score=66, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6319499, Length=372, Percent_Identity=37.3655913978495, Blast_Score=231, Evalue=9e-62,
Organism=Saccharomyces cerevisiae, GI6321773, Length=386, Percent_Identity=33.419689119171, Blast_Score=187, Evalue=2e-48,
Organism=Saccharomyces cerevisiae, GI6321649, Length=234, Percent_Identity=28.2051282051282, Blast_Score=75, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24640873, Length=365, Percent_Identity=36.1643835616438, Blast_Score=232, Evalue=2e-61,
Organism=Drosophila melanogaster, GI24640877, Length=365, Percent_Identity=36.1643835616438, Blast_Score=232, Evalue=2e-61,
Organism=Drosophila melanogaster, GI24640875, Length=365, Percent_Identity=36.1643835616438, Blast_Score=232, Evalue=2e-61,
Organism=Drosophila melanogaster, GI24640879, Length=323, Percent_Identity=34.6749226006192, Blast_Score=187, Evalue=1e-47,
Organism=Drosophila melanogaster, GI24585318, Length=102, Percent_Identity=37.2549019607843, Blast_Score=72, Evalue=4e-13,

Paralogues:

None

Copy number: 120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012675
- InterPro:   IPR004396
- InterPro:   IPR013029
- InterPro:   IPR006073
- InterPro:   IPR002917
- InterPro:   IPR012676
- InterPro:   IPR023192 [H]

Pfam domain/function: PF01926 MMR_HSR1; PF06071 YchF-GTPase_C [H]

EC number: NA

Molecular weight: Translated: 41095; Mature: 41095

Theoretical pI: Translated: 8.20; Mature: 8.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLKAGLIGLPNVGKSSLFSALTKMNVEIANYPFATIEPNIATVEIHDPRILQLTKIVKP
CCCCCCEEECCCCCHHHHHHHHHHCCEEEECCCCEEECCCEEEEEECCCCEEEHHHHCCC
EKTVFATYSFVDIAGLIKGASTGEGLGNKFLANVRNVDCLVHIVRCFQDPKIIHVNNEIN
CCEEEEEEHHHHHHHHHCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHCCCEEEEECCCCC
PIFDIQTINLELIFADLGTIQTIISRLAKKANNTNDKQVKFEFELAKKVEIHLKNGKSLR
CEEEEEEEEEEEEEECHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEEEEEEEECCCCCEE
DLELDSTEILQIKSQLLTIKPVLYVANLDQKSIQNPDANPYFKLKSYLVKEKAILLPLCI
EECCCCHHHHHHHHHHHEEEEEEEEECCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHH
ALEQEISQLDEEEKHLFLKEFLLEKSSLDLLILHSFYLLDLATFFTVGKKEVRSTFRKNT
HHHHHHHHCCHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHCCHHHHHHHHHCCC
PAIECSGIIHSDFKKKFIRVEIISFDDFVQAGSEKVVKENGKMRLEGKDYLIKDGEICHF
CCEEECCCHHHHHHCCEEEEEEECCHHHHHCCCHHHHHCCCCEEECCCCEEEECCCEEEE
RIGN
EECC
>Mature Secondary Structure
MNLKAGLIGLPNVGKSSLFSALTKMNVEIANYPFATIEPNIATVEIHDPRILQLTKIVKP
CCCCCCEEECCCCCHHHHHHHHHHCCEEEECCCCEEECCCEEEEEECCCCEEEHHHHCCC
EKTVFATYSFVDIAGLIKGASTGEGLGNKFLANVRNVDCLVHIVRCFQDPKIIHVNNEIN
CCEEEEEEHHHHHHHHHCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHCCCEEEEECCCCC
PIFDIQTINLELIFADLGTIQTIISRLAKKANNTNDKQVKFEFELAKKVEIHLKNGKSLR
CEEEEEEEEEEEEEECHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEEEEEEEECCCCCEE
DLELDSTEILQIKSQLLTIKPVLYVANLDQKSIQNPDANPYFKLKSYLVKEKAILLPLCI
EECCCCHHHHHHHHHHHEEEEEEEEECCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHH
ALEQEISQLDEEEKHLFLKEFLLEKSSLDLLILHSFYLLDLATFFTVGKKEVRSTFRKNT
HHHHHHHHCCHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHCCHHHHHHHHHCCC
PAIECSGIIHSDFKKKFIRVEIISFDDFVQAGSEKVVKENGKMRLEGKDYLIKDGEICHF
CCEEECCCHHHHHHCCEEEEEEECCHHHHHCCCHHHHHCCCCEEECCCCEEEECCCEEEE
RIGN
EECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]