Click here to switch to the map view.

The map label for this gene is trmD [H]

Identifier: 71893633

GI number: 71893633

Start: 331476

End: 332159

Strand: Reverse

Name: trmD [H]

Synonym: MHJ_0277

Alternate gene names: 71893633

Gene position: 332159-331476 (Counterclockwise)

Preceding gene: 71893634

Following gene: 71893632

Centisome position: 37.01

GC content: 30.7

Gene sequence:

>684_bases
ATGAAAATTAACATATTAACACTTTTTCCCCGTTATTTTGAGGTTTTCTGTAGAGAATCAATAATTGGAAAAGCAATTAA
GCAAAAAAAAATAACAATAAATGTTGTTAATTTTCGAGACTTTAGTAAAAATAAGCACAAAAAAGTTGATGACTATGTCT
ATGGTGGCGGTCCTGGTTTACTTTTACAAATTCAGCCAGTTGTTGATGCTCTTGAAAAGGTAGGTGGTCTTAAAATTGCT
CTTAGTCCACAAGGACAAAAATTTGATCAAAGCGTTGCGAGAAAGCTTGCAAAAGAAGATGAAATTACGATTTTATGTGG
TCATTATGAGGGTTTTGATCAAAGAATTATCGATAATTTTATTGATTTTGAATTATCACTAGGCGATTTTATTCTAACTG
GAGGCGAAATTGCTGCAATGGCAATAATTGATGCAATTATTCGGTTAAAACCAGATATAATAAACCCAGAATCACTTAAA
AATGAGACTTTTAATGATTTTTTATTAGATTTTCCGCAATATTCGCGACCAGCAAATTTTCGCGGCCTAGAAGTTCCTAA
AGTTCTAATTTCTGGAAATCATAGAGAGATTGGAGAATGACGTCAAGAACAGCGAGAGCTAATTACTAAAAAAAAACGAC
CAGATTTATGAGAAAAATTTTTAAAAATAAAAAATAAAAAATAA

Upstream 100 bases:

>100_bases
AACTCAAACCGTTAGAAATATTTTCTCTAAAAAGGGTGTTTTTAAAAATTTTCTAGAACAAAAATCATCAAGAGCTTAAT
TTTTTTTCTAAATACAACAG

Downstream 100 bases:

>100_bases
ATTAATTTTCCATTATAATTTTTTAAAAACAAATTAAAAATAGGGAGTTCAAAATGCAAGCAAAATTAATCGAAATTCTT
GAATCAAGCCAAATCCGTTT

Product: tRNA (guanine-N(1)-)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase [H]

Number of amino acids: Translated: 227; Mature: 227

Protein sequence:

>227_residues
MKINILTLFPRYFEVFCRESIIGKAIKQKKITINVVNFRDFSKNKHKKVDDYVYGGGPGLLLQIQPVVDALEKVGGLKIA
LSPQGQKFDQSVARKLAKEDEITILCGHYEGFDQRIIDNFIDFELSLGDFILTGGEIAAMAIIDAIIRLKPDIINPESLK
NETFNDFLLDFPQYSRPANFRGLEVPKVLISGNHREIGEWRQEQRELITKKKRPDLWEKFLKIKNKK

Sequences:

>Translated_227_residues
MKINILTLFPRYFEVFCRESIIGKAIKQKKITINVVNFRDFSKNKHKKVDDYVYGGGPGLLLQIQPVVDALEKVGGLKIA
LSPQGQKFDQSVARKLAKEDEITILCGHYEGFDQRIIDNFIDFELSLGDFILTGGEIAAMAIIDAIIRLKPDIINPESLK
NETFNDFLLDFPQYSRPANFRGLEVPKVLISGNHREIGE*RQEQRELITKKKRPDL*EKFLKIKNKK
>Mature_227_residues
MKINILTLFPRYFEVFCRESIIGKAIKQKKITINVVNFRDFSKNKHKKVDDYVYGGGPGLLLQIQPVVDALEKVGGLKIA
LSPQGQKFDQSVARKLAKEDEITILCGHYEGFDQRIIDNFIDFELSLGDFILTGGEIAAMAIIDAIIRLKPDIINPESLK
NETFNDFLLDFPQYSRPANFRGLEVPKVLISGNHREIGE*RQEQRELITKKKRPDL*EKFLKIKNKK

Specific function: Specifically methylates guanosine-37 in various tRNAs [H]

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family [H]

Homologues:

Organism=Escherichia coli, GI1788959, Length=233, Percent_Identity=39.4849785407725, Blast_Score=183, Evalue=9e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148 [H]

Pfam domain/function: PF01746 tRNA_m1G_MT [H]

EC number: =2.1.1.31 [H]

Molecular weight: Translated: 25713; Mature: 25713

Theoretical pI: Translated: 9.69; Mature: 9.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKINILTLFPRYFEVFCRESIIGKAIKQKKITINVVNFRDFSKNKHKKVDDYVYGGGPGL
CEEEEEECCHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCCCCCCCCCCCEEECCCCEE
LLQIQPVVDALEKVGGLKIALSPQGQKFDQSVARKLAKEDEITILCGHYEGFDQRIIDNF
EEEEHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHH
IDFELSLGDFILTGGEIAAMAIIDAIIRLKPDIINPESLKNETFNDFLLDFPQYSRPANF
HHCEEECCCEEEECCHHHHHHHHHHHHHCCCCCCCHHHHCCCCHHHHHHCCCCCCCCCCC
RGLEVPKVLISGNHREIGERQEQRELITKKKRPDLEKFLKIKNKK
CCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCC
>Mature Secondary Structure
MKINILTLFPRYFEVFCRESIIGKAIKQKKITINVVNFRDFSKNKHKKVDDYVYGGGPGL
CEEEEEECCHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCCCCCCCCCCCEEECCCCEE
LLQIQPVVDALEKVGGLKIALSPQGQKFDQSVARKLAKEDEITILCGHYEGFDQRIIDNF
EEEEHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHH
IDFELSLGDFILTGGEIAAMAIIDAIIRLKPDIINPESLKNETFNDFLLDFPQYSRPANF
HHCEEECCCEEEECCHHHHHHHHHHHHHCCCCCCCHHHHCCCCHHHHHHCCCCCCCCCCC
RGLEVPKVLISGNHREIGERQEQRELITKKKRPDLEKFLKIKNKK
CCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA