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The map label for this gene is topA [H]

Identifier: 71893631

GI number: 71893631

Start: 329142

End: 331064

Strand: Reverse

Name: topA [H]

Synonym: MHJ_0275

Alternate gene names: 71893631

Gene position: 331064-329142 (Counterclockwise)

Preceding gene: 71893632

Following gene: 71893630

Centisome position: 36.89

GC content: 30.68

Gene sequence:

>1923_bases
ATTTATAAAATCGTGGAAAAATTAATTATTGTCGAATCCCCTAATAAAATTAATACAATTTCATCGTATCTTGATTCAAG
TTATAAAGTTACAGCTTGCGTCGGTCATATCACAAATCTTGCTAAAACTGGACAGTTTGGTTTAGGAATTGATATTAACA
CTTGAACTCCATCTTATGTTCTTGACAAGACCAAAAAAAAAACAATTGATGAGATTAAAAAGTTAGCAAAACAAGCAAAA
ACTGTAATAATTGCCACCGATGCCGACCGCGAAGGCGAGGCAATTGGCGCTTCACTTGTCGAACATTTAGCCATTACTGA
TAAATACCAACGGATTAAATATAACGAAATTACCAAAACAGCAATACTCGAAGCCTTGGAAAACCCTGGTGAACTTAATC
AAAATTTAATAAATTCCCAACAAACAAGGCGAATGTTAGATCGAATTATTGGATTTCGGCTAACTGCCTTATTGAAAAAT
AAAATTAAAAATCCACCTAAAAATCCGGCAGCAGGTCGCGTTCAATCATTTGCTCTAAGCCTTATTTTTGACCGTGAAAA
AGAAATACGTGAATTTATACCTGAAAAATATTTTAATTTAGAAGTTAAAATTAATGACGAAAATGGTAATTTTTTTGATA
CAATTTATTGTAATCAACAAAATGAGGCAAAGAAAGTCAACTGAATTAATTCAGAAGAAGAACTTTCTGAGATAATTGCT
GAAATTAAAGAAAATCCTTATCTAATTTTTAAAAAATATAGCAAAGCAACCCGGAAGGACCCAAAAGTTATCCCGCTAAA
ACAGGCGACAATTTTTAAGAAAATGTCACAATATTCATCTGCTAGTGTCTCAAATGCGCTTCAAAAACTTTATGAGGGTT
ATGGCGATTATGGTTTGATTTCCTATCCGCGTACTGATTCAACAAGGCTAAGTCAAACTTTTATTGAAAAAGGTAAAGAA
TTTATTATTAAAAATTTTGGACCTAAATATTTTACTAAAAGTATTAAAGGATTTGCTGGCGATCAAGATGCCCATGAGGC
CATTAGACCAACAAATTTAGAGCTAAGTCCGGAACTAGCAAGAGAAAAATATCATTTAGGTCAAATTGAATTTCAAATTT
ATCAACTAATTTACCAGACAACAATTCAAGCCTTAATGAGATCTCCGGAACGAAAGATTACTAATTTGACTTTCAAAACA
CCTATAAAAGATCATATTTTTTCTTATAGTTATTCAGAGTTTACTTTTGATGGATATTATAAAGCCACTGAAAAACCAAG
TTTAAAATTTAAACCTGAATTTTCTGATATAGAAAGTTTAATTAGCAAAAAATTTCAGGTCGATAAAAATTTTGATAATT
CGATAAAATCTTCATTACATCAAACGAAACCGCCAGCCCATTTTCGTGATGGAACTTTAATTGAAAAATTAGATGATGAA
AAAGTGGGTCGCCCCTCAACTTTTGCAATTTCAGTAAAAAAATTAAAGGATCATTTATATGTTGATTCTGAATCAAAATC
GCTTGTTTTAAGTGATTTTGGCAAAATTGTCTATGAAAATTTAAAAATAATTTCGCCAGAAATTATTGATGCAAAATTTA
CAGCTCAAGTTGAAGAAGAACTTGATTTAATCGCTCAAGGGAAACAAGATTATAAAAAATATCTTGATTCAATGTGATCA
AAAATTGAAAATACTATAGAAAAGGCGGTTGAAGTTGAAAAGGTAATAATGGAAGCCAGAACTACTGGAGAAAAATGCAC
AGAATGTGATGCTGATTTGGTTTATCGTTATAATCGGAAAACAAATCAGCGTTTTATTGGCTGTTCTAGTTTCCCAAATT
GTCGCTTTATAAAAGTTGATGAAAATGCTAAAAAAGTTTACCGATTCAGAAAGAGAAATACCAAAATTTTAACAAATAAA
TAA

Upstream 100 bases:

>100_bases
ATCCGGTCAAATAAAGTTCGCCGCGCGAAATTATATTATATGAAAAAACGTTCTGGAAAGTCTGCTCGTCTTAAAGAAAT
TAAACGAAAAGAATTAAAAA

Downstream 100 bases:

>100_bases
TATATTTTTAAAAAAATATAAGAAAATGTTATGGTTGATACTAATTTAATTGTTGTAGTTGTTTTACTTGTAACTTTAAT
TATCGGTTTTTTTGCTTATA

Product: DNA topoisomerase I

Products: NA

Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme [H]

Number of amino acids: Translated: 640; Mature: 640

Protein sequence:

>640_residues
MYKIVEKLIIVESPNKINTISSYLDSSYKVTACVGHITNLAKTGQFGLGIDINTWTPSYVLDKTKKKTIDEIKKLAKQAK
TVIIATDADREGEAIGASLVEHLAITDKYQRIKYNEITKTAILEALENPGELNQNLINSQQTRRMLDRIIGFRLTALLKN
KIKNPPKNPAAGRVQSFALSLIFDREKEIREFIPEKYFNLEVKINDENGNFFDTIYCNQQNEAKKVNWINSEEELSEIIA
EIKENPYLIFKKYSKATRKDPKVIPLKQATIFKKMSQYSSASVSNALQKLYEGYGDYGLISYPRTDSTRLSQTFIEKGKE
FIIKNFGPKYFTKSIKGFAGDQDAHEAIRPTNLELSPELAREKYHLGQIEFQIYQLIYQTTIQALMRSPERKITNLTFKT
PIKDHIFSYSYSEFTFDGYYKATEKPSLKFKPEFSDIESLISKKFQVDKNFDNSIKSSLHQTKPPAHFRDGTLIEKLDDE
KVGRPSTFAISVKKLKDHLYVDSESKSLVLSDFGKIVYENLKIISPEIIDAKFTAQVEEELDLIAQGKQDYKKYLDSMWS
KIENTIEKAVEVEKVIMEARTTGEKCTECDADLVYRYNRKTNQRFIGCSSFPNCRFIKVDENAKKVYRFRKRNTKILTNK

Sequences:

>Translated_640_residues
MYKIVEKLIIVESPNKINTISSYLDSSYKVTACVGHITNLAKTGQFGLGIDINT*TPSYVLDKTKKKTIDEIKKLAKQAK
TVIIATDADREGEAIGASLVEHLAITDKYQRIKYNEITKTAILEALENPGELNQNLINSQQTRRMLDRIIGFRLTALLKN
KIKNPPKNPAAGRVQSFALSLIFDREKEIREFIPEKYFNLEVKINDENGNFFDTIYCNQQNEAKKVN*INSEEELSEIIA
EIKENPYLIFKKYSKATRKDPKVIPLKQATIFKKMSQYSSASVSNALQKLYEGYGDYGLISYPRTDSTRLSQTFIEKGKE
FIIKNFGPKYFTKSIKGFAGDQDAHEAIRPTNLELSPELAREKYHLGQIEFQIYQLIYQTTIQALMRSPERKITNLTFKT
PIKDHIFSYSYSEFTFDGYYKATEKPSLKFKPEFSDIESLISKKFQVDKNFDNSIKSSLHQTKPPAHFRDGTLIEKLDDE
KVGRPSTFAISVKKLKDHLYVDSESKSLVLSDFGKIVYENLKIISPEIIDAKFTAQVEEELDLIAQGKQDYKKYLDSM*S
KIENTIEKAVEVEKVIMEARTTGEKCTECDADLVYRYNRKTNQRFIGCSSFPNCRFIKVDENAKKVYRFRKRNTKILTNK
>Mature_640_residues
MYKIVEKLIIVESPNKINTISSYLDSSYKVTACVGHITNLAKTGQFGLGIDINT*TPSYVLDKTKKKTIDEIKKLAKQAK
TVIIATDADREGEAIGASLVEHLAITDKYQRIKYNEITKTAILEALENPGELNQNLINSQQTRRMLDRIIGFRLTALLKN
KIKNPPKNPAAGRVQSFALSLIFDREKEIREFIPEKYFNLEVKINDENGNFFDTIYCNQQNEAKKVN*INSEEELSEIIA
EIKENPYLIFKKYSKATRKDPKVIPLKQATIFKKMSQYSSASVSNALQKLYEGYGDYGLISYPRTDSTRLSQTFIEKGKE
FIIKNFGPKYFTKSIKGFAGDQDAHEAIRPTNLELSPELAREKYHLGQIEFQIYQLIYQTTIQALMRSPERKITNLTFKT
PIKDHIFSYSYSEFTFDGYYKATEKPSLKFKPEFSDIESLISKKFQVDKNFDNSIKSSLHQTKPPAHFRDGTLIEKLDDE
KVGRPSTFAISVKKLKDHLYVDSESKSLVLSDFGKIVYENLKIISPEIIDAKFTAQVEEELDLIAQGKQDYKKYLDSM*S
KIENTIEKAVEVEKVIMEARTTGEKCTECDADLVYRYNRKTNQRFIGCSSFPNCRFIKVDENAKKVYRFRKRNTKILTNK

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI4507635, Length=557, Percent_Identity=21.1849192100539, Blast_Score=93, Evalue=1e-18,
Organism=Escherichia coli, GI1787529, Length=641, Percent_Identity=30.7332293291732, Blast_Score=271, Evalue=1e-73,
Organism=Escherichia coli, GI1788061, Length=631, Percent_Identity=21.7115689381933, Blast_Score=73, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI32563869, Length=594, Percent_Identity=23.5690235690236, Blast_Score=114, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17555378, Length=576, Percent_Identity=22.9166666666667, Blast_Score=86, Evalue=6e-17,
Organism=Saccharomyces cerevisiae, GI6323263, Length=623, Percent_Identity=20.7062600321027, Blast_Score=84, Evalue=9e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR013498
- InterPro:   IPR005733
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim; PF01396 zf-C4_Topoisom [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 73149; Mature: 73149

Theoretical pI: Translated: 9.54; Mature: 9.54

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYKIVEKLIIVESPNKINTISSYLDSSYKVTACVGHITNLAKTGQFGLGIDINTTPSYVL
CHHHHHHHHEECCCCCHHHHHHHHCCCCEEHHHHHHHHHHHHCCCCCEEEECCCCCHHHH
DKTKKKTIDEIKKLAKQAKTVIIATDADREGEAIGASLVEHLAITDKYQRIKYNEITKTA
HHHHHHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ILEALENPGELNQNLINSQQTRRMLDRIIGFRLTALLKNKIKNPPKNPAAGRVQSFALSL
HHHHHCCCCHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHH
IFDREKEIREFIPEKYFNLEVKINDENGNFFDTIYCNQQNEAKKVNINSEEELSEIIAEI
HHCCHHHHHHHCCHHHEEEEEEEECCCCCEEEEEEECCCCCCCEECCCCHHHHHHHHHHH
KENPYLIFKKYSKATRKDPKVIPLKQATIFKKMSQYSSASVSNALQKLYEGYGDYGLISY
CCCCEEEEECHHHHCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEC
PRTDSTRLSQTFIEKGKEFIIKNFGPKYFTKSIKGFAGDQDAHEAIRPTNLELSPELARE
CCCCCHHHHHHHHHCCHHHHHHCCCHHHHHHHHCCCCCCCHHHHCCCCCCCCCCHHHHHH
KYHLGQIEFQIYQLIYQTTIQALMRSPERKITNLTFKTPIKDHIFSYSYSEFTFDGYYKA
HHHCCHHHHHHHHHHHHHHHHHHHHCCCHHHHCCEEECCHHHHHHCCCCCCEEECCEEEC
TEKPSLKFKPEFSDIESLISKKFQVDKNFDNSIKSSLHQTKPPAHFRDGTLIEKLDDEKV
CCCCCCEECCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHCCCCCC
GRPSTFAISVKKLKDHLYVDSESKSLVLSDFGKIVYENLKIISPEIIDAKFTAQVEEELD
CCCCEEEEEHHHHHHHHEECCCCCEEHHHHHHHHHHHHHHCCCCHHCCCHHHHHHHHHHH
LIAQGKQDYKKYLDSMSKIENTIEKAVEVEKVIMEARTTGEKCTECDADLVYRYNRKTNQ
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCHHHHEEECCCCCC
RFIGCSSFPNCRFIKVDENAKKVYRFRKRNTKILTNK
EEEECCCCCCCEEEEECCCHHHHHHHHHCCCEEECCC
>Mature Secondary Structure
MYKIVEKLIIVESPNKINTISSYLDSSYKVTACVGHITNLAKTGQFGLGIDINTTPSYVL
CHHHHHHHHEECCCCCHHHHHHHHCCCCEEHHHHHHHHHHHHCCCCCEEEECCCCCHHHH
DKTKKKTIDEIKKLAKQAKTVIIATDADREGEAIGASLVEHLAITDKYQRIKYNEITKTA
HHHHHHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ILEALENPGELNQNLINSQQTRRMLDRIIGFRLTALLKNKIKNPPKNPAAGRVQSFALSL
HHHHHCCCCHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHH
IFDREKEIREFIPEKYFNLEVKINDENGNFFDTIYCNQQNEAKKVNINSEEELSEIIAEI
HHCCHHHHHHHCCHHHEEEEEEEECCCCCEEEEEEECCCCCCCEECCCCHHHHHHHHHHH
KENPYLIFKKYSKATRKDPKVIPLKQATIFKKMSQYSSASVSNALQKLYEGYGDYGLISY
CCCCEEEEECHHHHCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEC
PRTDSTRLSQTFIEKGKEFIIKNFGPKYFTKSIKGFAGDQDAHEAIRPTNLELSPELARE
CCCCCHHHHHHHHHCCHHHHHHCCCHHHHHHHHCCCCCCCHHHHCCCCCCCCCCHHHHHH
KYHLGQIEFQIYQLIYQTTIQALMRSPERKITNLTFKTPIKDHIFSYSYSEFTFDGYYKA
HHHCCHHHHHHHHHHHHHHHHHHHHCCCHHHHCCEEECCHHHHHHCCCCCCEEECCEEEC
TEKPSLKFKPEFSDIESLISKKFQVDKNFDNSIKSSLHQTKPPAHFRDGTLIEKLDDEKV
CCCCCCEECCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHCCCCCC
GRPSTFAISVKKLKDHLYVDSESKSLVLSDFGKIVYENLKIISPEIIDAKFTAQVEEELD
CCCCEEEEEHHHHHHHHEECCCCCEEHHHHHHHHHHHHHHCCCCHHCCCHHHHHHHHHHH
LIAQGKQDYKKYLDSMSKIENTIEKAVEVEKVIMEARTTGEKCTECDADLVYRYNRKTNQ
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCHHHHEEECCCCCC
RFIGCSSFPNCRFIKVDENAKKVYRFRKRNTKILTNK
EEEECCCCCCCEEEEECCCHHHHHHHHHCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]