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The map label for this gene is lplA [H]

Identifier: 71893602

GI number: 71893602

Start: 295000

End: 296001

Strand: Reverse

Name: lplA [H]

Synonym: MHJ_0246

Alternate gene names: 71893602

Gene position: 296001-295000 (Counterclockwise)

Preceding gene: 71893603

Following gene: 71893601

Centisome position: 32.98

GC content: 27.45

Gene sequence:

>1002_bases
ATTATGAAAATTTATACAACAAAAAATACAAGCCCGTTTTATACACTAGTCTGTGAAGAAATTATTCTAAAAGATGAAGA
AAACCAAGAGGATATTCTTTACTTCTACCAACATAAAAATGCGATAATTATTGGAAAAAATCAAAATATTTACGAAGAAA
TTAAACTCGAGGAAGTTGAAGAGGAAAATATTGAAATCTACCGTCGGCTTTCAGGGGGTGGGGCGGTCTATCATGATCTA
GGTAATATAAATTTTTCCTTTATTAGTCAAAAGCAAAATCATAATTATCAAAAATTTCTATCACCAATTATCGAATTTTT
TCGATCCTTAGGGCTAAAAGCTGAATTTAAGGGACGAAATGATTTAATTGTCAATGGCGCAAAAGTTTCAGGTAATGCCC
AGATAATTTTTAAAAATAAAATTGTTCATCATGGAACAATTTTATTTAATGCTAATTTAGCAAAATTAGCACAAGTTTTA
AAGCCAAGTCGACTAAAAATTGAATCAAAAGGAATTAAATCTGTCCGCCAACGAGTAACAAACATTCTCTATGAAATGGA
AGAGCAAATTGATGATAATGAATTTATTTACCGTTTAATTTCATTTTTTGAAAAAAAATATCAAACAAAATCAGTTGATG
TTGAAAATCATTTTGATATAGGAATTTCAAATACTGAAAAATTCAAGGAAATAAGTCAATTACGAGAATCAAAAAACTGA
ATTTTTGGAACCAACCCATTTTTTAGTTATGAAAATATCAAACGAACAAGTGCCGGAATTTTGAAGGTCTTAGCAAATAT
TGAAAAAAATATAATTATAAAAATTAAGTTTGAAGGTGATTTTTTATCACAAAGGCCAATTGAAGAAATAGAAGAAATTT
TGGAAAATAAAGAATTTGACCGTGATAGTCTTGAAAGAGAACTAGAAAAAATTCATAATTTAAATGAGTATTTTGGTCAA
ATTCTGCCTTATGAAATTTTAGATACAATTTTTGGAGTTTAA

Upstream 100 bases:

>100_bases
TTAGAAAATTTGCGACTAGGCGAAAATCTAGCCGATTTTAAATCAAAATTTAAACTAATTACGACTTTTAATTTAGAATA
TAACTAAAATTTTGGAGATA

Downstream 100 bases:

>100_bases
ATGGTAGTAAATCGAAAAATTAAAATTGACAATGAAGAAATTTTCTACTTTTTAGAAGATAATAATCGACCAAAAGTACT
TTTTCTCCATGGTTTTAACT

Product: lipoate-protein ligase A

Products: NA

Alternate protein names: Lipoate--protein ligase [H]

Number of amino acids: Translated: 333; Mature: 333

Protein sequence:

>333_residues
MMKIYTTKNTSPFYTLVCEEIILKDEENQEDILYFYQHKNAIIIGKNQNIYEEIKLEEVEEENIEIYRRLSGGGAVYHDL
GNINFSFISQKQNHNYQKFLSPIIEFFRSLGLKAEFKGRNDLIVNGAKVSGNAQIIFKNKIVHHGTILFNANLAKLAQVL
KPSRLKIESKGIKSVRQRVTNILYEMEEQIDDNEFIYRLISFFEKKYQTKSVDVENHFDIGISNTEKFKEISQLRESKNW
IFGTNPFFSYENIKRTSAGILKVLANIEKNIIIKIKFEGDFLSQRPIEEIEEILENKEFDRDSLERELEKIHNLNEYFGQ
ILPYEILDTIFGV

Sequences:

>Translated_333_residues
MMKIYTTKNTSPFYTLVCEEIILKDEENQEDILYFYQHKNAIIIGKNQNIYEEIKLEEVEEENIEIYRRLSGGGAVYHDL
GNINFSFISQKQNHNYQKFLSPIIEFFRSLGLKAEFKGRNDLIVNGAKVSGNAQIIFKNKIVHHGTILFNANLAKLAQVL
KPSRLKIESKGIKSVRQRVTNILYEMEEQIDDNEFIYRLISFFEKKYQTKSVDVENHFDIGISNTEKFKEISQLRESKN*
IFGTNPFFSYENIKRTSAGILKVLANIEKNIIIKIKFEGDFLSQRPIEEIEEILENKEFDRDSLERELEKIHNLNEYFGQ
ILPYEILDTIFGV
>Mature_333_residues
MMKIYTTKNTSPFYTLVCEEIILKDEENQEDILYFYQHKNAIIIGKNQNIYEEIKLEEVEEENIEIYRRLSGGGAVYHDL
GNINFSFISQKQNHNYQKFLSPIIEFFRSLGLKAEFKGRNDLIVNGAKVSGNAQIIFKNKIVHHGTILFNANLAKLAQVL
KPSRLKIESKGIKSVRQRVTNILYEMEEQIDDNEFIYRLISFFEKKYQTKSVDVENHFDIGISNTEKFKEISQLRESKN*
IFGTNPFFSYENIKRTSAGILKVLANIEKNIIIKIKFEGDFLSQRPIEEIEEILENKEFDRDSLERELEKIHNLNEYFGQ
ILPYEILDTIFGV

Specific function: Catalyzes both the ATP-dependent activation of exogenously supplied lipoate to lipoyl-AMP and the transfer of the activated lipoyl onto the lipoyl domains of lipoate-dependent enzymes [H]

COG id: COG0095

COG function: function code H; Lipoate-protein ligase A

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lplA family [H]

Homologues:

Organism=Homo sapiens, GI7706252, Length=254, Percent_Identity=29.1338582677165, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI21729884, Length=254, Percent_Identity=29.1338582677165, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI21729882, Length=254, Percent_Identity=29.1338582677165, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI21729880, Length=254, Percent_Identity=29.1338582677165, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI21729878, Length=254, Percent_Identity=29.1338582677165, Blast_Score=103, Evalue=3e-22,
Organism=Escherichia coli, GI1790846, Length=314, Percent_Identity=31.5286624203822, Blast_Score=167, Evalue=9e-43,
Organism=Caenorhabditis elegans, GI71981909, Length=255, Percent_Identity=29.0196078431373, Blast_Score=106, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6322415, Length=293, Percent_Identity=31.3993174061433, Blast_Score=131, Evalue=1e-31,
Organism=Drosophila melanogaster, GI28573464, Length=182, Percent_Identity=30.7692307692308, Blast_Score=99, Evalue=3e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004143
- InterPro:   IPR005107
- InterPro:   IPR019491
- InterPro:   IPR004562 [H]

Pfam domain/function: PF03099 BPL_LipA_LipB; PF10437 Lip_prot_lig_C [H]

EC number: =2.7.7.63 [H]

Molecular weight: Translated: 38889; Mature: 38889

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMKIYTTKNTSPFYTLVCEEIILKDEENQEDILYFYQHKNAIIIGKNQNIYEEIKLEEVE
CEEEEECCCCCCHHHHHHHHHHHCCCCCCCCEEEEEECCCEEEEECCCCHHHHHHHHHHH
EENIEIYRRLSGGGAVYHDLGNINFSFISQKQNHNYQKFLSPIIEFFRSLGLKAEFKGRN
HCCHHHHEEECCCCEEEECCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCEEEECCCC
DLIVNGAKVSGNAQIIFKNKIVHHGTILFNANLAKLAQVLKPSRLKIESKGIKSVRQRVT
CEEEECEEECCCEEEEEECCEEECEEEEEECCHHHHHHHHCCCCEEEHHHHHHHHHHHHH
NILYEMEEQIDDNEFIYRLISFFEKKYQTKSVDVENHFDIGISNTEKFKEISQLRESKNI
HHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCC
FGTNPFFSYENIKRTSAGILKVLANIEKNIIIKIKFEGDFLSQRPIEEIEEILENKEFDR
CCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCHHHHHHHHHCCCCCH
DSLERELEKIHNLNEYFGQILPYEILDTIFGV
HHHHHHHHHHHCHHHHHHHCCCHHHHHHHHCC
>Mature Secondary Structure
MMKIYTTKNTSPFYTLVCEEIILKDEENQEDILYFYQHKNAIIIGKNQNIYEEIKLEEVE
CEEEEECCCCCCHHHHHHHHHHHCCCCCCCCEEEEEECCCEEEEECCCCHHHHHHHHHHH
EENIEIYRRLSGGGAVYHDLGNINFSFISQKQNHNYQKFLSPIIEFFRSLGLKAEFKGRN
HCCHHHHEEECCCCEEEECCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCEEEECCCC
DLIVNGAKVSGNAQIIFKNKIVHHGTILFNANLAKLAQVLKPSRLKIESKGIKSVRQRVT
CEEEECEEECCCEEEEEECCEEECEEEEEECCHHHHHHHHCCCCEEEHHHHHHHHHHHHH
NILYEMEEQIDDNEFIYRLISFFEKKYQTKSVDVENHFDIGISNTEKFKEISQLRESKNI
HHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCC
FGTNPFFSYENIKRTSAGILKVLANIEKNIIIKIKFEGDFLSQRPIEEIEEILENKEFDR
CCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCHHHHHHHHHCCCCCH
DSLERELEKIHNLNEYFGQILPYEILDTIFGV
HHHHHHHHHHHCHHHHHHHCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]