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The map label for this gene is clpB [H]

Identifier: 71893457

GI number: 71893457

Start: 129445

End: 131628

Strand: Reverse

Name: clpB [H]

Synonym: MHJ_0098

Alternate gene names: 71893457

Gene position: 131628-129445 (Counterclockwise)

Preceding gene: 71893460

Following gene: 71893451

Centisome position: 14.67

GC content: 29.76

Gene sequence:

>2184_bases
ATTTTTTCATTTAAGAAAAAAAATTGGCACTCTTTACTTTTAAGTGCCAAAAAATATGTTATAATTTATTTTGTTAAGAA
AAATAAAAAATTAAAAAGGAAAAAAATGAAACAAAAACTCGAAAATCTTGAAAAATACGCAAGAAATTTAACAAAATTAG
CAAAAGAAAATAAAATTGAACCTGTTATCGGCCGCGATGACGAAATTAGAAGGATTATCAAAATTTTATCCCGAAAAACA
AAAAATAATCCTGTCCTTGTCGGGGATCCTGGAGTGGGTAAGACTGCAATTGTTGAAGGAATGGCTCTAAAGATTATTGC
AAATCAAGTACCGGATAATTTAAAAAATAAACAAATTTTTGAATTAGATCTAACCTTAATTCTTGCAGGTGCTTCTTTTA
AAGGTGAATTCGAAAAACGAATTAAGGCAATTTTGCAAGAAATTGAGCAAAATTCCGATCAAGTTATTATTTTTATTGAT
GAAATTCACCTTCTAATTGGAACAGGATCTTCTGGGACTGATTCAATGGATTTTGCCAATATCCTAAAACCAATTATGGC
TCGCGGACAGATTAAATTAATCGGGGCTACCACAAATTCCGAATATCGCTTATATATCGAAAAAGATGGCGCCCTTGAAA
GAAGAATGCAAAAAGTAGAAATTTTAGAGCCTTCAGTTATTGATACAATTAATATTTTACGGGGAATTAAGGAAAGGCTA
GAAAATTTCCATCAAGTAAAAATTAAGGATTCTGCTCTTGTTTTTGCTACAAAAGCGGCAAATCGTTACATTTTTGACCG
CTTTCTACCTGATAAAGCTATCGATTTAGTCGATGAAGCTGCTGCTTCTTTAAAAGTTGAAATCAACTACCAACCAGAAA
AACTTGAAAAAGCAAAGCGCGAGCTAATTTATTTAAAAATGGAAGAAATTAACTCGCAAAAACAAGATAATTCAGAATTA
AAATCCAAAATTGAAAATCTTGAAAATGAAGTAAAAAAATTACAAGATCAATGGGATCAATCAAAAAAATCAGCCTCTGA
AATCGCTAGCTTATCCCAGGAACTTGAAAAACTAAAATATCAACAAAATTACTTAATGGAACAAGGAGACTACCAAAAAG
CCGCCGAGATTAAATACGGAAAAATTCCCAAAATAAGTAAAAAATTAGGCGAAATTAAAGCAAGAAGGCAGGAAATTTCC
AATGTTCTAGACGAAAGTCAGATCGCAAAGGTTGTCTCTAATTGAACAAAAATTCCGATTGAAAAACTTTTAGAATCAGA
AATTCAAAAATATTTGAATTTAGAAAAAAATTTAGCAAAATCGCTTAAGGGTCAAAATCAGGCAATTAAGGCTGTTTCAG
ATGCGATTTTGCGGTTTAAAGCTAAAATTAATGATGAATCCCGCCCAATTTCATCATTTTTCTTTATGGGACCAACTGGG
GTGGGAAAAACTGAACTTGCTAGAGCTTTAGCTCTTAATTTATTTAATAATAAAAACCAAATAATCCGTCTTGATATGTC
AGAATATATGGAAAAACATAGTGTTTCAAAGCTAATTGGGGCTCCTCCGGGTTATATTGGTTTTGAACAAGGTGGTAATC
TAACAAATAAAGTAAGACTAAATCCTTATTCGATTATTTTGCTTGATGAAATTGAAAAAGCTCATCCGGAAGTAATCAAC
ATTTTTTTACAAATTCTTGATAATGGTGAAATTGTTGATAGTAAGTCACAAAAAGTAAATTTTCGCAATACAATTATAAT
TATGACCTCAAATATCGGTGCTAATAAAATTCTTGAGGGTAAAAAGATGAATGAAATTGAGGCAAAAAAGGAACTTTTAA
GATATTTAAAGCCAGAATTTCTCAACCGAATTGATGAAATTATCGTATTTAATCCTTTAAATTATGATATAATTTTTGAA
ATTATTGAACTTGAACTAAAGGATTTGCAAAATCGTCTAAAGGAAAATAATTTTGAGATTGAATTTGAAAAATCAGTCAA
AAATTGAATTTTAGAGTTTGGATATGATAAAAATTTTGGTGCCAGGCCAATTAAGCGCTTTATTAAGAAAGAAATTGAAA
ATTTTGTTGCCAAAAAAATAGTGGCCGAAGAAATTTTAAAAGATAAAAAATACAATTTATCTTTTAAAAATGATAAATTG
CATCTTAATGAAAGCGAAAATTAA

Upstream 100 bases:

>100_bases
TTTCCAATAATAATTTTTTTCATAATAAATATTAATCAATTATATCATTTTTTCGTTTAATTTAAAGGTAAAAAATTTAA
ATTATCCTTTAAATTAAAGT

Downstream 100 bases:

>100_bases
TCTTTTATTTTAAAGAATTTTCCAAAGCTGTTCTAAAAGTTTGTTTATCCTCAAACTGATAATCAAATTGTGTGAAAAAT
TTAAACAAAACCTCGGTATT

Product: ATP-dependent protease binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 727; Mature: 727

Protein sequence:

>727_residues
MFSFKKKNWHSLLLSAKKYVIIYFVKKNKKLKRKKMKQKLENLEKYARNLTKLAKENKIEPVIGRDDEIRRIIKILSRKT
KNNPVLVGDPGVGKTAIVEGMALKIIANQVPDNLKNKQIFELDLTLILAGASFKGEFEKRIKAILQEIEQNSDQVIIFID
EIHLLIGTGSSGTDSMDFANILKPIMARGQIKLIGATTNSEYRLYIEKDGALERRMQKVEILEPSVIDTINILRGIKERL
ENFHQVKIKDSALVFATKAANRYIFDRFLPDKAIDLVDEAAASLKVEINYQPEKLEKAKRELIYLKMEEINSQKQDNSEL
KSKIENLENEVKKLQDQWDQSKKSASEIASLSQELEKLKYQQNYLMEQGDYQKAAEIKYGKIPKISKKLGEIKARRQEIS
NVLDESQIAKVVSNWTKIPIEKLLESEIQKYLNLEKNLAKSLKGQNQAIKAVSDAILRFKAKINDESRPISSFFFMGPTG
VGKTELARALALNLFNNKNQIIRLDMSEYMEKHSVSKLIGAPPGYIGFEQGGNLTNKVRLNPYSIILLDEIEKAHPEVIN
IFLQILDNGEIVDSKSQKVNFRNTIIIMTSNIGANKILEGKKMNEIEAKKELLRYLKPEFLNRIDEIIVFNPLNYDIIFE
IIELELKDLQNRLKENNFEIEFEKSVKNWILEFGYDKNFGARPIKRFIKKEIENFVAKKIVAEEILKDKKYNLSFKNDKL
HLNESEN

Sequences:

>Translated_727_residues
MFSFKKKNWHSLLLSAKKYVIIYFVKKNKKLKRKKMKQKLENLEKYARNLTKLAKENKIEPVIGRDDEIRRIIKILSRKT
KNNPVLVGDPGVGKTAIVEGMALKIIANQVPDNLKNKQIFELDLTLILAGASFKGEFEKRIKAILQEIEQNSDQVIIFID
EIHLLIGTGSSGTDSMDFANILKPIMARGQIKLIGATTNSEYRLYIEKDGALERRMQKVEILEPSVIDTINILRGIKERL
ENFHQVKIKDSALVFATKAANRYIFDRFLPDKAIDLVDEAAASLKVEINYQPEKLEKAKRELIYLKMEEINSQKQDNSEL
KSKIENLENEVKKLQDQWDQSKKSASEIASLSQELEKLKYQQNYLMEQGDYQKAAEIKYGKIPKISKKLGEIKARRQEIS
NVLDESQIAKVVSN*TKIPIEKLLESEIQKYLNLEKNLAKSLKGQNQAIKAVSDAILRFKAKINDESRPISSFFFMGPTG
VGKTELARALALNLFNNKNQIIRLDMSEYMEKHSVSKLIGAPPGYIGFEQGGNLTNKVRLNPYSIILLDEIEKAHPEVIN
IFLQILDNGEIVDSKSQKVNFRNTIIIMTSNIGANKILEGKKMNEIEAKKELLRYLKPEFLNRIDEIIVFNPLNYDIIFE
IIELELKDLQNRLKENNFEIEFEKSVKN*ILEFGYDKNFGARPIKRFIKKEIENFVAKKIVAEEILKDKKYNLSFKNDKL
HLNESEN
>Mature_727_residues
MFSFKKKNWHSLLLSAKKYVIIYFVKKNKKLKRKKMKQKLENLEKYARNLTKLAKENKIEPVIGRDDEIRRIIKILSRKT
KNNPVLVGDPGVGKTAIVEGMALKIIANQVPDNLKNKQIFELDLTLILAGASFKGEFEKRIKAILQEIEQNSDQVIIFID
EIHLLIGTGSSGTDSMDFANILKPIMARGQIKLIGATTNSEYRLYIEKDGALERRMQKVEILEPSVIDTINILRGIKERL
ENFHQVKIKDSALVFATKAANRYIFDRFLPDKAIDLVDEAAASLKVEINYQPEKLEKAKRELIYLKMEEINSQKQDNSEL
KSKIENLENEVKKLQDQWDQSKKSASEIASLSQELEKLKYQQNYLMEQGDYQKAAEIKYGKIPKISKKLGEIKARRQEIS
NVLDESQIAKVVSN*TKIPIEKLLESEIQKYLNLEKNLAKSLKGQNQAIKAVSDAILRFKAKINDESRPISSFFFMGPTG
VGKTELARALALNLFNNKNQIIRLDMSEYMEKHSVSKLIGAPPGYIGFEQGGNLTNKVRLNPYSIILLDEIEKAHPEVIN
IFLQILDNGEIVDSKSQKVNFRNTIIIMTSNIGANKILEGKKMNEIEAKKELLRYLKPEFLNRIDEIIVFNPLNYDIIFE
IIELELKDLQNRLKENNFEIEFEKSVKN*ILEFGYDKNFGARPIKRFIKKEIENFVAKKIVAEEILKDKKYNLSFKNDKL
HLNESEN

Specific function: Part of a stress-induced multi-chaperone system, it is involved in the recovery of the cell from heat-induced damage, in cooperation with DnaK, DnaJ and GrpE. Acts before DnaK, in the processing of protein aggregates. Protein binding stimulates the ATPase

COG id: COG0542

COG function: function code O; ATPases with chaperone activity, ATP-binding subunit

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the clpA/clpB family [H]

Homologues:

Organism=Homo sapiens, GI13540606, Length=298, Percent_Identity=34.2281879194631, Blast_Score=183, Evalue=5e-46,
Organism=Escherichia coli, GI1788943, Length=697, Percent_Identity=46.6284074605452, Blast_Score=651, Evalue=0.0,
Organism=Escherichia coli, GI1787109, Length=336, Percent_Identity=38.3928571428571, Blast_Score=245, Evalue=6e-66,
Organism=Saccharomyces cerevisiae, GI6320464, Length=702, Percent_Identity=44.3019943019943, Blast_Score=591, Evalue=1e-169,
Organism=Saccharomyces cerevisiae, GI6323002, Length=708, Percent_Identity=40.5367231638418, Blast_Score=537, Evalue=1e-153,

Paralogues:

None

Copy number: 560 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013093
- InterPro:   IPR003959
- InterPro:   IPR018368
- InterPro:   IPR017730
- InterPro:   IPR001270
- InterPro:   IPR019489
- InterPro:   IPR004176
- InterPro:   IPR023150 [H]

Pfam domain/function: PF00004 AAA; PF07724 AAA_2; PF02861 Clp_N; PF10431 ClpB_D2-small [H]

EC number: NA

Molecular weight: Translated: 83434; Mature: 83434

Theoretical pI: Translated: 9.97; Mature: 9.97

Prosite motif: PS00871 CLPAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFSFKKKNWHSLLLSAKKYVIIYFVKKNKKLKRKKMKQKLENLEKYARNLTKLAKENKIE
CCCCCCCHHHHHHHCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PVIGRDDEIRRIIKILSRKTKNNPVLVGDPGVGKTAIVEGMALKIIANQVPDNLKNKQIF
CCCCCCHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEE
ELDLTLILAGASFKGEFEKRIKAILQEIEQNSDQVIIFIDEIHLLIGTGSSGTDSMDFAN
EEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEEEEEEEECCCCCCCCHHHHH
ILKPIMARGQIKLIGATTNSEYRLYIEKDGALERRMQKVEILEPSVIDTINILRGIKERL
HHHHHHCCCCEEEEEEECCCCEEEEEECCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
ENFHQVKIKDSALVFATKAANRYIFDRFLPDKAIDLVDEAAASLKVEINYQPEKLEKAKR
HHHHEEEEECCEEEEEEHHCCHHHHHHHCCHHHHHHHHHHHHEEEEEECCCHHHHHHHHH
ELIYLKMEEINSQKQDNSELKSKIENLENEVKKLQDQWDQSKKSASEIASLSQELEKLKY
HHEEEEHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QQNYLMEQGDYQKAAEIKYGKIPKISKKLGEIKARRQEISNVLDESQIAKVVSNTKIPIE
HHHHHHCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
KLLESEIQKYLNLEKNLAKSLKGQNQAIKAVSDAILRFKAKINDESRPISSFFFMGPTGV
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHEEEECCCCC
GKTELARALALNLFNNKNQIIRLDMSEYMEKHSVSKLIGAPPGYIGFEQGGNLTNKVRLN
CHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEC
PYSIILLDEIEKAHPEVINIFLQILDNGEIVDSKSQKVNFRNTIIIMTSNIGANKILEGK
CEEEEEEECHHHHHHHHHHHHHHHHCCCCEECCCCCCEEECEEEEEEECCCCCCCCCCCC
KMNEIEAKKELLRYLKPEFLNRIDEIIVFNPLNYDIIFEIIELELKDLQNRLKENNFEIE
CCHHHHHHHHHHHHHCHHHHHHHHHEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCEEE
FEKSVKNILEFGYDKNFGARPIKRFIKKEIENFVAKKIVAEEILKDKKYNLSFKNDKLHL
EHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCEEEC
NESEN
CCCCC
>Mature Secondary Structure
MFSFKKKNWHSLLLSAKKYVIIYFVKKNKKLKRKKMKQKLENLEKYARNLTKLAKENKIE
CCCCCCCHHHHHHHCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PVIGRDDEIRRIIKILSRKTKNNPVLVGDPGVGKTAIVEGMALKIIANQVPDNLKNKQIF
CCCCCCHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEE
ELDLTLILAGASFKGEFEKRIKAILQEIEQNSDQVIIFIDEIHLLIGTGSSGTDSMDFAN
EEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEEEEEEEECCCCCCCCHHHHH
ILKPIMARGQIKLIGATTNSEYRLYIEKDGALERRMQKVEILEPSVIDTINILRGIKERL
HHHHHHCCCCEEEEEEECCCCEEEEEECCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
ENFHQVKIKDSALVFATKAANRYIFDRFLPDKAIDLVDEAAASLKVEINYQPEKLEKAKR
HHHHEEEEECCEEEEEEHHCCHHHHHHHCCHHHHHHHHHHHHEEEEEECCCHHHHHHHHH
ELIYLKMEEINSQKQDNSELKSKIENLENEVKKLQDQWDQSKKSASEIASLSQELEKLKY
HHEEEEHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QQNYLMEQGDYQKAAEIKYGKIPKISKKLGEIKARRQEISNVLDESQIAKVVSNTKIPIE
HHHHHHCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
KLLESEIQKYLNLEKNLAKSLKGQNQAIKAVSDAILRFKAKINDESRPISSFFFMGPTGV
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHEEEECCCCC
GKTELARALALNLFNNKNQIIRLDMSEYMEKHSVSKLIGAPPGYIGFEQGGNLTNKVRLN
CHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEC
PYSIILLDEIEKAHPEVINIFLQILDNGEIVDSKSQKVNFRNTIIIMTSNIGANKILEGK
CEEEEEEECHHHHHHHHHHHHHHHHCCCCEECCCCCCEEECEEEEEEECCCCCCCCCCCC
KMNEIEAKKELLRYLKPEFLNRIDEIIVFNPLNYDIIFEIIELELKDLQNRLKENNFEIE
CCHHHHHHHHHHHHHCHHHHHHHHHEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCEEE
FEKSVKNILEFGYDKNFGARPIKRFIKKEIENFVAKKIVAEEILKDKKYNLSFKNDKLHL
EHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCEEEC
NESEN
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Serine endopeptidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12240834 [H]