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The map label for this gene is pfkA [H]

Identifier: 71892365

GI number: 71892365

Start: 746684

End: 747652

Strand: Reverse

Name: pfkA [H]

Synonym: BPEN_624

Alternate gene names: 71892365

Gene position: 747652-746684 (Counterclockwise)

Preceding gene: 71892366

Following gene: 71892362

Centisome position: 94.44

GC content: 37.98

Gene sequence:

>969_bases
ATGTTTATGATAAAAAAAATAGGAGTATTAACAAGCGGAGGGGATTCTCCAGGAATGAATGCTGCTATTCGCGGCGTAGT
ACGGGCAGGTCTTTCTGAAGGAATAGAAGTGTATGGAATACATGACGGTTATTTAGGATTATTTCAAGATCGAATGATAC
AATTGAGTCGTCGTAGTGTTTCAGATATAATAAATCGTGGAGGAACTTTTCTTGGTTCTGCTCGTTTTCCTGAATTTAAG
GAAGATGCCACTAGGACAATAGTTATCAACAATATTAATCAACGTAGTCTCGATGCTCTCATAATAATTGGAGGAGATGG
ATCTTATTTAGGTGCTAAGAGATTGAGTGATATAGGATTTCCTTGCATCGGATTGCCTGGGACTATTGATAACGATGTTG
CCGGAACTGATTACACCATCGGTTATTTTACAGCTTTAGAAACAATTGTTGATGCAATTGATAGGTTACGCGACACATCT
TCTTCGCATCAACGTATTTCTATTGTAGAGGTAATGGGACGTTGTTGCGGTGATTTGACTATGGCAGCAGCAATAGCTGG
AGGTTGTGAATTTATTATAGTCCCCGAAGTAGAATTTAACCCACAAGATCTAGTAAACGAGATTAAATCTGGTATTTCTA
AAGGGAAAAAACATGCTATAGTAGCTATCACAGAACGTATTTGTAATGTATTCTATTTAGCGCGATACATTGAAGAAAAG
ACTGGTAGAGAGACTCGTGCTACAGTTTTAGGATACATCCAACGTGGAGGCAAGCCTGTTGCATATGATCGCATTCTAGC
ATCTCGGATGGGAGCATATTCTATTGATCTGCTATTAAAGGGTTATGTAGGGCGCTGTATTGGGGTACAAAATGAAAAAT
TGGTACATCATGATATTAATGATGCTATACAACATATGCAACGTCCATTCCGTCGAGATTTATTGAAAACAGCCAAAAAA
CTATTTTAA

Upstream 100 bases:

>100_bases
TATTTTTGTATATTTATATAATAATTATATTTTTATTATTATTTATTTTATATTTCAACATGATTATAGTCTGCTGCACA
TAACAACATAATAGAGTTCT

Downstream 100 bases:

>100_bases
GTTATTTAAAATCATCATAAATAACTTGTTTCTTAAATAAAAATATTATTTTTACATAATCAGATTATCTTTGCTTATAT
AAATATCACTTATTTAGTGT

Product: 6-phosphofructokinase

Products: NA

Alternate protein names: Phosphofructokinase; Phosphohexokinase [H]

Number of amino acids: Translated: 322; Mature: 322

Protein sequence:

>322_residues
MFMIKKIGVLTSGGDSPGMNAAIRGVVRAGLSEGIEVYGIHDGYLGLFQDRMIQLSRRSVSDIINRGGTFLGSARFPEFK
EDATRTIVINNINQRSLDALIIIGGDGSYLGAKRLSDIGFPCIGLPGTIDNDVAGTDYTIGYFTALETIVDAIDRLRDTS
SSHQRISIVEVMGRCCGDLTMAAAIAGGCEFIIVPEVEFNPQDLVNEIKSGISKGKKHAIVAITERICNVFYLARYIEEK
TGRETRATVLGYIQRGGKPVAYDRILASRMGAYSIDLLLKGYVGRCIGVQNEKLVHHDINDAIQHMQRPFRRDLLKTAKK
LF

Sequences:

>Translated_322_residues
MFMIKKIGVLTSGGDSPGMNAAIRGVVRAGLSEGIEVYGIHDGYLGLFQDRMIQLSRRSVSDIINRGGTFLGSARFPEFK
EDATRTIVINNINQRSLDALIIIGGDGSYLGAKRLSDIGFPCIGLPGTIDNDVAGTDYTIGYFTALETIVDAIDRLRDTS
SSHQRISIVEVMGRCCGDLTMAAAIAGGCEFIIVPEVEFNPQDLVNEIKSGISKGKKHAIVAITERICNVFYLARYIEEK
TGRETRATVLGYIQRGGKPVAYDRILASRMGAYSIDLLLKGYVGRCIGVQNEKLVHHDINDAIQHMQRPFRRDLLKTAKK
LF
>Mature_322_residues
MFMIKKIGVLTSGGDSPGMNAAIRGVVRAGLSEGIEVYGIHDGYLGLFQDRMIQLSRRSVSDIINRGGTFLGSARFPEFK
EDATRTIVINNINQRSLDALIIIGGDGSYLGAKRLSDIGFPCIGLPGTIDNDVAGTDYTIGYFTALETIVDAIDRLRDTS
SSHQRISIVEVMGRCCGDLTMAAAIAGGCEFIIVPEVEFNPQDLVNEIKSGISKGKKHAIVAITERICNVFYLARYIEEK
TGRETRATVLGYIQRGGKPVAYDRILASRMGAYSIDLLLKGYVGRCIGVQNEKLVHHDINDAIQHMQRPFRRDLLKTAKK
LF

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family [H]

Homologues:

Organism=Homo sapiens, GI48762920, Length=313, Percent_Identity=38.3386581469649, Blast_Score=190, Evalue=2e-48,
Organism=Homo sapiens, GI266453619, Length=314, Percent_Identity=37.2611464968153, Blast_Score=189, Evalue=3e-48,
Organism=Homo sapiens, GI266453768, Length=314, Percent_Identity=37.2611464968153, Blast_Score=189, Evalue=3e-48,
Organism=Homo sapiens, GI266453748, Length=314, Percent_Identity=37.2611464968153, Blast_Score=189, Evalue=3e-48,
Organism=Homo sapiens, GI4505749, Length=314, Percent_Identity=37.2611464968153, Blast_Score=189, Evalue=3e-48,
Organism=Homo sapiens, GI11321601, Length=311, Percent_Identity=37.9421221864952, Blast_Score=186, Evalue=2e-47,
Organism=Escherichia coli, GI1790350, Length=320, Percent_Identity=75.625, Blast_Score=518, Evalue=1e-148,
Organism=Caenorhabditis elegans, GI25147584, Length=315, Percent_Identity=36.5079365079365, Blast_Score=184, Evalue=5e-47,
Organism=Caenorhabditis elegans, GI17558788, Length=308, Percent_Identity=35.3896103896104, Blast_Score=173, Evalue=9e-44,
Organism=Caenorhabditis elegans, GI72003601, Length=293, Percent_Identity=28.6689419795222, Blast_Score=129, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6321679, Length=312, Percent_Identity=36.2179487179487, Blast_Score=182, Evalue=5e-47,
Organism=Saccharomyces cerevisiae, GI6323861, Length=340, Percent_Identity=32.6470588235294, Blast_Score=169, Evalue=5e-43,
Organism=Drosophila melanogaster, GI17647809, Length=315, Percent_Identity=39.0476190476191, Blast_Score=196, Evalue=1e-50,
Organism=Drosophila melanogaster, GI28573326, Length=312, Percent_Identity=40.0641025641026, Blast_Score=196, Evalue=2e-50,
Organism=Drosophila melanogaster, GI24652337, Length=311, Percent_Identity=39.2282958199357, Blast_Score=195, Evalue=4e-50,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012003
- InterPro:   IPR012828
- InterPro:   IPR022953
- InterPro:   IPR015912
- InterPro:   IPR000023 [H]

Pfam domain/function: PF00365 PFK [H]

EC number: =2.7.1.11 [H]

Molecular weight: Translated: 35309; Mature: 35309

Theoretical pI: Translated: 8.55; Mature: 8.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFMIKKIGVLTSGGDSPGMNAAIRGVVRAGLSEGIEVYGIHDGYLGLFQDRMIQLSRRSV
CCEEEEECEEECCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHH
SDIINRGGTFLGSARFPEFKEDATRTIVINNINQRSLDALIIIGGDGSYLGAKRLSDIGF
HHHHHCCCCEEECCCCCCCHHCCCEEEEEECCCCCCCCEEEEECCCCCCHHHHHHHHCCC
PCIGLPGTIDNDVAGTDYTIGYFTALETIVDAIDRLRDTSSSHQRISIVEVMGRCCGDLT
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
MAAAIAGGCEFIIVPEVEFNPQDLVNEIKSGISKGKKHAIVAITERICNVFYLARYIEEK
HHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
TGRETRATVLGYIQRGGKPVAYDRILASRMGAYSIDLLLKGYVGRCIGVQNEKLVHHDIN
CCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHH
DAIQHMQRPFRRDLLKTAKKLF
HHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MFMIKKIGVLTSGGDSPGMNAAIRGVVRAGLSEGIEVYGIHDGYLGLFQDRMIQLSRRSV
CCEEEEECEEECCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHH
SDIINRGGTFLGSARFPEFKEDATRTIVINNINQRSLDALIIIGGDGSYLGAKRLSDIGF
HHHHHCCCCEEECCCCCCCHHCCCEEEEEECCCCCCCCEEEEECCCCCCHHHHHHHHCCC
PCIGLPGTIDNDVAGTDYTIGYFTALETIVDAIDRLRDTSSSHQRISIVEVMGRCCGDLT
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
MAAAIAGGCEFIIVPEVEFNPQDLVNEIKSGISKGKKHAIVAITERICNVFYLARYIEEK
HHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
TGRETRATVLGYIQRGGKPVAYDRILASRMGAYSIDLLLKGYVGRCIGVQNEKLVHHDIN
CCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHH
DAIQHMQRPFRRDLLKTAKKLF
HHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA