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The map label for this gene is dapF

Identifier: 71892348

GI number: 71892348

Start: 722069

End: 722893

Strand: Reverse

Name: dapF

Synonym: BPEN_600

Alternate gene names: 71892348

Gene position: 722893-722069 (Counterclockwise)

Preceding gene: 71892351

Following gene: 71892347

Centisome position: 91.31

GC content: 33.82

Gene sequence:

>825_bases
ATGCGATTTACCAAAATGAACGGGCTGGGCAATGACTTTGTCATCATAGATGCTGTCACTCAAAATATACATCTTACTTC
TGAAAATATCAGATATTTGTCAGATCGATTTTATGGAGTTGGTTTTGATCAATTGTTAATAGTAGAACCCCCTTATGATC
CAGCAATAGATTTTCATTGTCGAATTTATAATTCGGACGGAACAGAAGTAAATCAATGTGGTAATGGTATGCGTTGTGTT
GCGCAATTTGTCTGCTTAAAAAAATTAACTAATAAACGAAACATTCATATTAGTACTCGCGCTAACCATATAATATTGTC
TATTATGAATGACAATCGCGTATCTGTTAATATGGGGGCGCCTATTTTTGATCCAAAACTTATTCCGTTTTATATATCAC
AATATCAAAAAACTTATATTTTATTTTTACCTACACAAATAATACTATGTGGTGTAGTATCTATGGGTAATCCTCATTGT
ATTATTTTAGTAGAAAAAATAGAAAATATCCAAGTTACTTCATTAGGATCAGCATTAGAAGATCATCATTGTTTTCCAGA
ACGAGTCAATGTAAGTTTTATGCAAATTATTAACTGTAATAATATTCGATTACGAGTATATGAGCGCGGAGTAGGAGAAA
CTCAAGCTTGCGGAACTGCCGCCTGTGCGGCAGTTGCCGTAGGCATTCAACAGGGATTATTGTATGAATCAGTAAACGTA
AACTTGCCTGGGGGTACTATATCAGTAAATTGGAAAGGGGCAAGTAATGCACTGTACATGACTGGATCAACGTCTTATGT
ATATGATGGATATATTAATTTATAA

Upstream 100 bases:

>100_bases
ATGTTGTAATAAATACAATAAAATCAATAATAGTTAAGTGTCATAAAACACTAAAACCATTTCCAAAAATCAGTGATGTG
TGATGCTATGTGAGGAAGGT

Downstream 100 bases:

>100_bases
ATATATAGTAAATCCACCAAAATGTTATTGTTTTTTAATTCACGTATTTTATATTGAAACTTTATTTAGTATTTAATTTT
TTATACATTATATGAATATA

Product: diaminopimelate epimerase

Products: NA

Alternate protein names: DAP epimerase

Number of amino acids: Translated: 274; Mature: 274

Protein sequence:

>274_residues
MRFTKMNGLGNDFVIIDAVTQNIHLTSENIRYLSDRFYGVGFDQLLIVEPPYDPAIDFHCRIYNSDGTEVNQCGNGMRCV
AQFVCLKKLTNKRNIHISTRANHIILSIMNDNRVSVNMGAPIFDPKLIPFYISQYQKTYILFLPTQIILCGVVSMGNPHC
IILVEKIENIQVTSLGSALEDHHCFPERVNVSFMQIINCNNIRLRVYERGVGETQACGTAACAAVAVGIQQGLLYESVNV
NLPGGTISVNWKGASNALYMTGSTSYVYDGYINL

Sequences:

>Translated_274_residues
MRFTKMNGLGNDFVIIDAVTQNIHLTSENIRYLSDRFYGVGFDQLLIVEPPYDPAIDFHCRIYNSDGTEVNQCGNGMRCV
AQFVCLKKLTNKRNIHISTRANHIILSIMNDNRVSVNMGAPIFDPKLIPFYISQYQKTYILFLPTQIILCGVVSMGNPHC
IILVEKIENIQVTSLGSALEDHHCFPERVNVSFMQIINCNNIRLRVYERGVGETQACGTAACAAVAVGIQQGLLYESVNV
NLPGGTISVNWKGASNALYMTGSTSYVYDGYINL
>Mature_274_residues
MRFTKMNGLGNDFVIIDAVTQNIHLTSENIRYLSDRFYGVGFDQLLIVEPPYDPAIDFHCRIYNSDGTEVNQCGNGMRCV
AQFVCLKKLTNKRNIHISTRANHIILSIMNDNRVSVNMGAPIFDPKLIPFYISQYQKTYILFLPTQIILCGVVSMGNPHC
IILVEKIENIQVTSLGSALEDHHCFPERVNVSFMQIINCNNIRLRVYERGVGETQACGTAACAAVAVGIQQGLLYESVNV
NLPGGTISVNWKGASNALYMTGSTSYVYDGYINL

Specific function: Biosynthesis of lysine from aspartate semialdehyde; sixth step. [C]

COG id: COG0253

COG function: function code E; Diaminopimelate epimerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diaminopimelate epimerase family

Homologues:

Organism=Escherichia coli, GI87082334, Length=274, Percent_Identity=59.8540145985402, Blast_Score=366, Evalue=1e-103,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DAPF_BLOPB (Q491Z7)

Other databases:

- EMBL:   CP000016
- RefSeq:   YP_278082.1
- HSSP:   P44859
- ProteinModelPortal:   Q491Z7
- SMR:   Q491Z7
- STRING:   Q491Z7
- GeneID:   3563073
- GenomeReviews:   CP000016_GR
- KEGG:   bpn:BPEN_600
- eggNOG:   COG0253
- HOGENOM:   HBG399442
- OMA:   HTGIGFD
- ProtClustDB:   PRK00450
- BioCyc:   CBLO291272:BPEN_600-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00197
- InterPro:   IPR001653
- InterPro:   IPR018510
- TIGRFAMs:   TIGR00652

Pfam domain/function: PF01678 DAP_epimerase

EC number: =5.1.1.7

Molecular weight: Translated: 30517; Mature: 30517

Theoretical pI: Translated: 7.10; Mature: 7.10

Prosite motif: PS01326 DAP_EPIMERASE

Important sites: ACT_SITE 73-73 ACT_SITE 217-217

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
3.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFTKMNGLGNDFVIIDAVTQNIHLTSENIRYLSDRFYGVGFDQLLIVEPPYDPAIDFHC
CCEEECCCCCCCEEEEEEEECEEEEECCHHHHHHHHCCCCCCCEEEEEECCCCCCEEEEE
RIYNSDGTEVNQCGNGMRCVAQFVCLKKLTNKRNIHISTRANHIILSIMNDNRVSVNMGA
EEECCCCCCHHHCCCHHHHHHHHHHHHHHCCCCEEEEEECCCEEEEEEECCCEEEEEECC
PIFDPKLIPFYISQYQKTYILFLPTQIILCGVVSMGNPHCIILVEKIENIQVTSLGSALE
CCCCCCHHHHHHHCCCEEEEEEECHHEEEHHHHCCCCCEEEEEEEECCCEEEEECCCHHH
DHHCFPERVNVSFMQIINCNNIRLRVYERGVGETQACGTAACAAVAVGIQQGLLYESVNV
HCCCCCCCCCEEEEEEEECCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHCCEEEEEEEE
NLPGGTISVNWKGASNALYMTGSTSYVYDGYINL
ECCCCEEEEEEECCCCEEEEECCCCEEEEEEEEC
>Mature Secondary Structure
MRFTKMNGLGNDFVIIDAVTQNIHLTSENIRYLSDRFYGVGFDQLLIVEPPYDPAIDFHC
CCEEECCCCCCCEEEEEEEECEEEEECCHHHHHHHHCCCCCCCEEEEEECCCCCCEEEEE
RIYNSDGTEVNQCGNGMRCVAQFVCLKKLTNKRNIHISTRANHIILSIMNDNRVSVNMGA
EEECCCCCCHHHCCCHHHHHHHHHHHHHHCCCCEEEEEECCCEEEEEEECCCEEEEEECC
PIFDPKLIPFYISQYQKTYILFLPTQIILCGVVSMGNPHCIILVEKIENIQVTSLGSALE
CCCCCCHHHHHHHCCCEEEEEEECHHEEEHHHHCCCCCEEEEEEEECCCEEEEECCCHHH
DHHCFPERVNVSFMQIINCNNIRLRVYERGVGETQACGTAACAAVAVGIQQGLLYESVNV
HCCCCCCCCCEEEEEEEECCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHCCEEEEEEEE
NLPGGTISVNWKGASNALYMTGSTSYVYDGYINL
ECCCCEEEEEEECCCCEEEEECCCCEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA