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The map label for this gene is tuf

Identifier: 71892333

GI number: 71892333

Start: 700810

End: 701994

Strand: Reverse

Name: tuf

Synonym: BPEN_584

Alternate gene names: 71892333

Gene position: 701994-700810 (Counterclockwise)

Preceding gene: 71892334

Following gene: 71892332

Centisome position: 88.67

GC content: 37.3

Gene sequence:

>1185_bases
GTGTCCAAAGAAAAATTTAAACGTATAAAGCCACATATTAATGTAGGCACTATTGGCCATGTAGATCACGGAAAAACTAC
TTTAACTGCCGCTATAACCACTGTTTTATCCAAAAAATATGGTGGTTACGCACGTGCTTTTGATCAAATTGACAATGCTC
CAGAAGAAAAAGCACGAGGTATTACTATAAATACTTCTCATGTTGAATACGATACTGCATATCGGCATTATGCGCATGTT
GACTGTCCTGGACATGCCGATTATATAAAAAACATGATAACTGGAGCTGCGCAAATGGATGGTGCAATTTTAGTAGTAGC
CGCTACTGATGGGCCGATGCCGCAAACCCGTGAACATATTTTATTAGCTAGACAAGTAGGAGTACCCCACATCATTGTAT
TCATGAATAAATGCGACATGGTAAATGATGAAGAATTATTAGAATTGGTTGAAATGGAAATGCGAGAATTACTATCTCAA
TACGATTTTCCAGGAGACAATACGCCAATTATTCGCGGATCTGCTTTAAAAGCATTAGAAAATGATGAAATATGGTCTAA
TAAAATTTTAGAATTATCGGATGCACTGGATAATTATATCCCAGAACCTAAACGTGTTGTAGATCAACCGTTTTTACTAC
CCATTGAAGATGTATTTTCTATTTCTGGACGAGGTACGGTTGTAACAGGTCGTATTGAACGTGGTGTTATCAGAGTAGGA
GAAGAAATAGAAATTGTTGGTATAAAAGATACAATAAAAACCACATGTACCGGAGTAGAAATGTTTAGAAAATTATTAGA
CGAAGGACGTGCTGGAGAAAATGTTGGTGTTTTATTACGGGGCACTAAACGAGATGAAGTAGAGCGTGGTCAAGTATTAT
CGAAACCTGGGTATATCAAGCCACATTCTCATTTTGAATCAGAAGTGTACATTTTAAATAAAGATGAAGGAGGAAGGCAT
ACTCCTTTTTTCAAAGGGTATCGTCCTCAATTTTATTTTCGCACTACAGATATCACTGGCACCATTGAATTACCAGAAGG
AGCGGATATGGTGATGCCTGGCGATAATATTAGAATGATCGTTCATTTAATTGCCCCAATAGCTATGGATGACGGTCTTA
GATTTGCTATTAGAGAGGGGGGTCGTACTATAGGCGCTGGTATTGTATCTAAAATTATATCCTAA

Upstream 100 bases:

>100_bases
GCGCAAATTATCATAAATTCTCGTCAAATTAAGCAATAATAGGTAATATTTAGGTTCAATTATTTTTGAAAAACCATAAA
AAGACCTAAGGAGTTTAAAG

Downstream 100 bases:

>100_bases
TTACATAAATCAATAGATATAATTTTTTAATCATATGATAGGGATGTAAAGAATGAAAGTAAAAAAAAAATGTATGTATT
AGAAATAATTAAATGGATTA

Product: elongation factor Tu

Products: GDP; phosphate

Alternate protein names: EF-Tu

Number of amino acids: Translated: 394; Mature: 393

Protein sequence:

>394_residues
MSKEKFKRIKPHINVGTIGHVDHGKTTLTAAITTVLSKKYGGYARAFDQIDNAPEEKARGITINTSHVEYDTAYRHYAHV
DCPGHADYIKNMITGAAQMDGAILVVAATDGPMPQTREHILLARQVGVPHIIVFMNKCDMVNDEELLELVEMEMRELLSQ
YDFPGDNTPIIRGSALKALENDEIWSNKILELSDALDNYIPEPKRVVDQPFLLPIEDVFSISGRGTVVTGRIERGVIRVG
EEIEIVGIKDTIKTTCTGVEMFRKLLDEGRAGENVGVLLRGTKRDEVERGQVLSKPGYIKPHSHFESEVYILNKDEGGRH
TPFFKGYRPQFYFRTTDITGTIELPEGADMVMPGDNIRMIVHLIAPIAMDDGLRFAIREGGRTIGAGIVSKIIS

Sequences:

>Translated_394_residues
MSKEKFKRIKPHINVGTIGHVDHGKTTLTAAITTVLSKKYGGYARAFDQIDNAPEEKARGITINTSHVEYDTAYRHYAHV
DCPGHADYIKNMITGAAQMDGAILVVAATDGPMPQTREHILLARQVGVPHIIVFMNKCDMVNDEELLELVEMEMRELLSQ
YDFPGDNTPIIRGSALKALENDEIWSNKILELSDALDNYIPEPKRVVDQPFLLPIEDVFSISGRGTVVTGRIERGVIRVG
EEIEIVGIKDTIKTTCTGVEMFRKLLDEGRAGENVGVLLRGTKRDEVERGQVLSKPGYIKPHSHFESEVYILNKDEGGRH
TPFFKGYRPQFYFRTTDITGTIELPEGADMVMPGDNIRMIVHLIAPIAMDDGLRFAIREGGRTIGAGIVSKIIS
>Mature_393_residues
SKEKFKRIKPHINVGTIGHVDHGKTTLTAAITTVLSKKYGGYARAFDQIDNAPEEKARGITINTSHVEYDTAYRHYAHVD
CPGHADYIKNMITGAAQMDGAILVVAATDGPMPQTREHILLARQVGVPHIIVFMNKCDMVNDEELLELVEMEMRELLSQY
DFPGDNTPIIRGSALKALENDEIWSNKILELSDALDNYIPEPKRVVDQPFLLPIEDVFSISGRGTVVTGRIERGVIRVGE
EIEIVGIKDTIKTTCTGVEMFRKLLDEGRAGENVGVLLRGTKRDEVERGQVLSKPGYIKPHSHFESEVYILNKDEGGRHT
PFFKGYRPQFYFRTTDITGTIELPEGADMVMPGDNIRMIVHLIAPIAMDDGLRFAIREGGRTIGAGIVSKIIS

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily

Homologues:

Organism=Homo sapiens, GI34147630, Length=395, Percent_Identity=54.6835443037975, Blast_Score=466, Evalue=1e-131,
Organism=Homo sapiens, GI223555963, Length=440, Percent_Identity=30, Blast_Score=162, Evalue=7e-40,
Organism=Homo sapiens, GI5729864, Length=440, Percent_Identity=30, Blast_Score=161, Evalue=9e-40,
Organism=Homo sapiens, GI4503475, Length=354, Percent_Identity=31.9209039548023, Blast_Score=151, Evalue=1e-36,
Organism=Homo sapiens, GI4503471, Length=449, Percent_Identity=28.9532293986637, Blast_Score=148, Evalue=1e-35,
Organism=Homo sapiens, GI194018522, Length=335, Percent_Identity=31.044776119403, Blast_Score=120, Evalue=3e-27,
Organism=Homo sapiens, GI194018520, Length=335, Percent_Identity=31.044776119403, Blast_Score=119, Evalue=4e-27,
Organism=Homo sapiens, GI194097354, Length=335, Percent_Identity=31.044776119403, Blast_Score=119, Evalue=4e-27,
Organism=Homo sapiens, GI46094014, Length=321, Percent_Identity=30.5295950155763, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI54607086, Length=313, Percent_Identity=29.3929712460064, Blast_Score=112, Evalue=7e-25,
Organism=Homo sapiens, GI4503507, Length=270, Percent_Identity=28.5185185185185, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI94966754, Length=135, Percent_Identity=33.3333333333333, Blast_Score=69, Evalue=8e-12,
Organism=Homo sapiens, GI53729339, Length=235, Percent_Identity=32.3404255319149, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI53729337, Length=235, Percent_Identity=32.3404255319149, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1790412, Length=394, Percent_Identity=85.7868020304569, Blast_Score=687, Evalue=0.0,
Organism=Escherichia coli, GI1789737, Length=393, Percent_Identity=85.7506361323155, Blast_Score=685, Evalue=0.0,
Organism=Escherichia coli, GI2367247, Length=370, Percent_Identity=28.1081081081081, Blast_Score=132, Evalue=3e-32,
Organism=Escherichia coli, GI1789108, Length=311, Percent_Identity=28.6173633440514, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI48994988, Length=245, Percent_Identity=31.8367346938775, Blast_Score=91, Evalue=9e-20,
Organism=Caenorhabditis elegans, GI17556456, Length=399, Percent_Identity=52.3809523809524, Blast_Score=398, Evalue=1e-111,
Organism=Caenorhabditis elegans, GI25141371, Length=387, Percent_Identity=47.2868217054264, Blast_Score=346, Evalue=1e-95,
Organism=Caenorhabditis elegans, GI17552884, Length=463, Percent_Identity=30.8855291576674, Blast_Score=152, Evalue=3e-37,
Organism=Caenorhabditis elegans, GI17569207, Length=463, Percent_Identity=30.8855291576674, Blast_Score=152, Evalue=3e-37,
Organism=Caenorhabditis elegans, GI32566303, Length=445, Percent_Identity=29.438202247191, Blast_Score=133, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI17506081, Length=271, Percent_Identity=32.1033210332103, Blast_Score=131, Evalue=8e-31,
Organism=Caenorhabditis elegans, GI115532067, Length=437, Percent_Identity=28.1464530892449, Blast_Score=126, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI115532065, Length=434, Percent_Identity=28.110599078341, Blast_Score=125, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI32566629, Length=439, Percent_Identity=24.8291571753986, Blast_Score=113, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17509919, Length=266, Percent_Identity=29.6992481203008, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI32566301, Length=161, Percent_Identity=36.0248447204969, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17557151, Length=236, Percent_Identity=27.9661016949153, Blast_Score=79, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6324761, Length=395, Percent_Identity=63.0379746835443, Blast_Score=513, Evalue=1e-146,
Organism=Saccharomyces cerevisiae, GI6325337, Length=353, Percent_Identity=31.728045325779, Blast_Score=146, Evalue=5e-36,
Organism=Saccharomyces cerevisiae, GI6319594, Length=353, Percent_Identity=31.728045325779, Blast_Score=146, Evalue=5e-36,
Organism=Saccharomyces cerevisiae, GI6322937, Length=364, Percent_Identity=26.6483516483516, Blast_Score=98, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6320863, Length=263, Percent_Identity=29.6577946768061, Blast_Score=95, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6320377, Length=339, Percent_Identity=28.6135693215339, Blast_Score=84, Evalue=5e-17,
Organism=Saccharomyces cerevisiae, GI6323320, Length=255, Percent_Identity=27.843137254902, Blast_Score=67, Evalue=4e-12,
Organism=Drosophila melanogaster, GI281363316, Length=393, Percent_Identity=56.9974554707379, Blast_Score=477, Evalue=1e-135,
Organism=Drosophila melanogaster, GI17864358, Length=393, Percent_Identity=56.9974554707379, Blast_Score=477, Evalue=1e-135,
Organism=Drosophila melanogaster, GI19921738, Length=383, Percent_Identity=52.4804177545692, Blast_Score=403, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24652838, Length=458, Percent_Identity=29.2576419213974, Blast_Score=151, Evalue=9e-37,
Organism=Drosophila melanogaster, GI17137572, Length=458, Percent_Identity=29.2576419213974, Blast_Score=151, Evalue=9e-37,
Organism=Drosophila melanogaster, GI45553807, Length=461, Percent_Identity=28.4164859002169, Blast_Score=149, Evalue=2e-36,
Organism=Drosophila melanogaster, GI45553816, Length=461, Percent_Identity=28.4164859002169, Blast_Score=149, Evalue=2e-36,
Organism=Drosophila melanogaster, GI24651721, Length=461, Percent_Identity=28.4164859002169, Blast_Score=149, Evalue=2e-36,
Organism=Drosophila melanogaster, GI17864154, Length=461, Percent_Identity=28.4164859002169, Blast_Score=149, Evalue=2e-36,
Organism=Drosophila melanogaster, GI19922690, Length=302, Percent_Identity=30.4635761589404, Blast_Score=127, Evalue=9e-30,
Organism=Drosophila melanogaster, GI45550900, Length=435, Percent_Identity=27.5862068965517, Blast_Score=122, Evalue=3e-28,
Organism=Drosophila melanogaster, GI17137380, Length=345, Percent_Identity=29.5652173913043, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24647048, Length=278, Percent_Identity=29.136690647482, Blast_Score=96, Evalue=4e-20,
Organism=Drosophila melanogaster, GI24647046, Length=429, Percent_Identity=27.7389277389277, Blast_Score=96, Evalue=4e-20,
Organism=Drosophila melanogaster, GI28572034, Length=165, Percent_Identity=34.5454545454545, Blast_Score=65, Evalue=6e-11,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): EFTU_BLOPB (Q492B2)

Other databases:

- EMBL:   CP000016
- RefSeq:   YP_278067.1
- HSSP:   P0A6N1
- ProteinModelPortal:   Q492B2
- SMR:   Q492B2
- STRING:   Q492B2
- GeneID:   3563134
- GenomeReviews:   CP000016_GR
- KEGG:   bpn:BPEN_584
- eggNOG:   COG0050
- HOGENOM:   HBG307581
- OMA:   FEGQVYV
- ProtClustDB:   PRK00049
- BioCyc:   CBLO291272:BPEN_584-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00118_B
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000
- PANTHER:   PTHR23115:SF31
- PRINTS:   PR00315
- TIGRFAMs:   TIGR00485
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3; SSF50465 Elong_init_C; SSF50447 Translat_factor

EC number: 3.6.5.3

Molecular weight: Translated: 43842; Mature: 43711

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKEKFKRIKPHINVGTIGHVDHGKTTLTAAITTVLSKKYGGYARAFDQIDNAPEEKARG
CCHHHHHHHCCCCCCCEEECCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHCC
ITINTSHVEYDTAYRHYAHVDCPGHADYIKNMITGAAQMDGAILVVAATDGPMPQTREHI
EEEECCCEEECHHHHEEEEECCCCHHHHHHHHHHCHHHCCCEEEEEEECCCCCCCCHHHH
LLARQVGVPHIIVFMNKCDMVNDEELLELVEMEMRELLSQYDFPGDNTPIIRGSALKALE
HHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECHHHHHCC
NDEIWSNKILELSDALDNYIPEPKRVVDQPFLLPIEDVFSISGRGTVVTGRIERGVIRVG
CCCHHHHHHHHHHHHHHHHCCCCHHHHCCCCEEEHHHHHCCCCCCEEEEECCCCCEEECC
EEIEIVGIKDTIKTTCTGVEMFRKLLDEGRAGENVGVLLRGTKRDEVERGQVLSKPGYIK
CEEEEEEECHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHCCEECCCCCCC
PHSHFESEVYILNKDEGGRHTPFFKGYRPQFYFRTTDITGTIELPEGADMVMPGDNIRMI
CCCCCCCEEEEEECCCCCCCCCCCCCCCCEEEEEEECCEEEEECCCCCCEEECCCCHHHH
VHLIAPIAMDDGLRFAIREGGRTIGAGIVSKIIS
HHHHHHHHHCCCCEEEECCCCCEEHHHHHHHHCC
>Mature Secondary Structure 
SKEKFKRIKPHINVGTIGHVDHGKTTLTAAITTVLSKKYGGYARAFDQIDNAPEEKARG
CHHHHHHHCCCCCCCEEECCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHCC
ITINTSHVEYDTAYRHYAHVDCPGHADYIKNMITGAAQMDGAILVVAATDGPMPQTREHI
EEEECCCEEECHHHHEEEEECCCCHHHHHHHHHHCHHHCCCEEEEEEECCCCCCCCHHHH
LLARQVGVPHIIVFMNKCDMVNDEELLELVEMEMRELLSQYDFPGDNTPIIRGSALKALE
HHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECHHHHHCC
NDEIWSNKILELSDALDNYIPEPKRVVDQPFLLPIEDVFSISGRGTVVTGRIERGVIRVG
CCCHHHHHHHHHHHHHHHHCCCCHHHHCCCCEEEHHHHHCCCCCCEEEEECCCCCEEECC
EEIEIVGIKDTIKTTCTGVEMFRKLLDEGRAGENVGVLLRGTKRDEVERGQVLSKPGYIK
CEEEEEEECHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHCCEECCCCCCC
PHSHFESEVYILNKDEGGRHTPFFKGYRPQFYFRTTDITGTIELPEGADMVMPGDNIRMI
CCCCCCCEEEEEECCCCCCCCCCCCCCCCEEEEEEECCEEEEECCCCCCEEECCCCHHHH
VHLIAPIAMDDGLRFAIREGGRTIGAGIVSKIIS
HHHHHHHHHCCCCEEEECCCCCEEHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA