Definition Candidatus Blochmannia pennsylvanicus str. BPEN, complete genome.
Accession NC_007292
Length 791,654

Click here to switch to the map view.

The map label for this gene is guaA

Identifier: 71892299

GI number: 71892299

Start: 650857

End: 652434

Strand: Reverse

Name: guaA

Synonym: BPEN_546

Alternate gene names: 71892299

Gene position: 652434-650857 (Counterclockwise)

Preceding gene: 71892300

Following gene: 71892298

Centisome position: 82.41

GC content: 32.0

Gene sequence:

>1578_bases
GTGAAATGTAACAATCATCGTATTTTAGTAATAGATTTTGGATCTCAATATACTCAGTTGTTGTTGAGAAGAATTCGAGA
ATTAGGAGTATATTCTGAATCTTGTTCTTGGAACATCAGTAAATCTCAAGTATATGCATTTAATCCTAATGGTATTATTC
TTTCTGGAGGTCCTTATAGTGTTATCGATAATGATTCTCCGTATATTCCTGAATTTGTGTTTCAATTAGGTATACCTATA
TTTGGAATTTGTTATGGTATGCAAATTATGTCGTTTCAATTAGGTGGACGAGTAGAACGTGTTATTGCACAGCGTGAATT
TGGTTGTACTCAAGTAACAATTCTTTCTAAAAGCGTTTTTATAAATGATATCTATGATTATGTTGATGATGTTACGGGGC
GTTTTGCTCTTGATGTATGGATGAGTCACGGGGACGTTGTTACTACTGTTCCCAAAGATTTTACGGTTATTGGTGTTAAT
AAGTATCGACAAGTTGCTATGATGGCTAATGAAGATCGTCATTTATATGGTGTTCAATTTCACCCGGAAGTTACCCATAC
TAAAAAAGGAAAAAGTATTTTAGAACGTTTTATTACGTGTATTTGTCGCTGTAAATCTTCTTGGAAAATAGCGAATATTA
TTGATGATATTGTTATGGATACTCGTGTAAAAGTAGGTAATGATAAAGTAGTGCTTGGGTTTTCAGGTGGGATTGATTCC
CTTGTTACAGCTTTATTATTACGACGCGCAATTGGTCATCAATTTATCTGTATTTATATTGATAATGGGTTATTATCAAA
TTATGAATTTGATCGAATTAAAAATTTTTGTGCTCTAAATTGTAATTTAGATATTATTTATTTGTCGCAAGAACAACGAT
TTTTCAATGCTTTAATTGGAGTTAATGATCCAGAAAAAAAAAGAAAAATAATTGGTAGAGTTTTTACAGAAGTTTTTGAA
GAGCAGATTCGTAATTTAGTAGCTGTAAAATGGTTAGCTCAAGGTACTATATATTCAGATGTTATTGAATCTGGTGTATC
TTTATCTTCTTTTAAAAATGTGATTAAATCGCATCATAATGTAGGTGGTTTTTGTGGAATTACGGATATTCAATTACTCG
AGCCTATAAGAAATTTATTTAAAGATGAAGTTCGCAGTATTGGATTAGATTTAGGGATACCTTATGATATAGCTTATCGT
CATCCGTTTCCAGGACCTGGATTGGCTATTAGAATATTAGGTGAAGTAAAAAAAGAATATTGTGATATTTTACGTAGAGT
GGATTTTATTTTTATCGAAGAACTTAAGCGTGAACATTTATATTCCAAAATTAGTCAGGCCTTCGCAGTATTTTTACCAA
CTCATTCAGTAGGAATACAGGGTGATCAGCGTAAGTATAAGTGGGTAATTGCTCTTCGTGCAGTGGAAACTATAGATTTT
ATGACAGCACATTGGGCTTATTTATCTTATGATTTTTTAAATAAGGTATCTAATCGTATCGTCAATGAAGTTGAGGAAGT
ATCTCGTGTGGTGTATGATATTTCCTCAAAACCTCCAGCTACTATTGAATGGGAATAA

Upstream 100 bases:

>100_bases
ATTTTTGCAGTTTGTGCGGCGTGGTGTCACGAACGTTGTTGATCATTAATATGTGGTTGATCAATGATCGATTAATATAA
ATAATAGAATAAGCTATAAT

Downstream 100 bases:

>100_bases
ACGTATTATACAGTATATAGGTTAAATATTTTTGTGTACTTTTATTGTTTTATGTGACATACAATTTAAAGTATGTACTC
CCACTATGGAAGTACATACT

Product: GMP synthase

Products: NA

Alternate protein names: GMP synthetase; Glutamine amidotransferase

Number of amino acids: Translated: 525; Mature: 525

Protein sequence:

>525_residues
MKCNNHRILVIDFGSQYTQLLLRRIRELGVYSESCSWNISKSQVYAFNPNGIILSGGPYSVIDNDSPYIPEFVFQLGIPI
FGICYGMQIMSFQLGGRVERVIAQREFGCTQVTILSKSVFINDIYDYVDDVTGRFALDVWMSHGDVVTTVPKDFTVIGVN
KYRQVAMMANEDRHLYGVQFHPEVTHTKKGKSILERFITCICRCKSSWKIANIIDDIVMDTRVKVGNDKVVLGFSGGIDS
LVTALLLRRAIGHQFICIYIDNGLLSNYEFDRIKNFCALNCNLDIIYLSQEQRFFNALIGVNDPEKKRKIIGRVFTEVFE
EQIRNLVAVKWLAQGTIYSDVIESGVSLSSFKNVIKSHHNVGGFCGITDIQLLEPIRNLFKDEVRSIGLDLGIPYDIAYR
HPFPGPGLAIRILGEVKKEYCDILRRVDFIFIEELKREHLYSKISQAFAVFLPTHSVGIQGDQRKYKWVIALRAVETIDF
MTAHWAYLSYDFLNKVSNRIVNEVEEVSRVVYDISSKPPATIEWE

Sequences:

>Translated_525_residues
MKCNNHRILVIDFGSQYTQLLLRRIRELGVYSESCSWNISKSQVYAFNPNGIILSGGPYSVIDNDSPYIPEFVFQLGIPI
FGICYGMQIMSFQLGGRVERVIAQREFGCTQVTILSKSVFINDIYDYVDDVTGRFALDVWMSHGDVVTTVPKDFTVIGVN
KYRQVAMMANEDRHLYGVQFHPEVTHTKKGKSILERFITCICRCKSSWKIANIIDDIVMDTRVKVGNDKVVLGFSGGIDS
LVTALLLRRAIGHQFICIYIDNGLLSNYEFDRIKNFCALNCNLDIIYLSQEQRFFNALIGVNDPEKKRKIIGRVFTEVFE
EQIRNLVAVKWLAQGTIYSDVIESGVSLSSFKNVIKSHHNVGGFCGITDIQLLEPIRNLFKDEVRSIGLDLGIPYDIAYR
HPFPGPGLAIRILGEVKKEYCDILRRVDFIFIEELKREHLYSKISQAFAVFLPTHSVGIQGDQRKYKWVIALRAVETIDF
MTAHWAYLSYDFLNKVSNRIVNEVEEVSRVVYDISSKPPATIEWE
>Mature_525_residues
MKCNNHRILVIDFGSQYTQLLLRRIRELGVYSESCSWNISKSQVYAFNPNGIILSGGPYSVIDNDSPYIPEFVFQLGIPI
FGICYGMQIMSFQLGGRVERVIAQREFGCTQVTILSKSVFINDIYDYVDDVTGRFALDVWMSHGDVVTTVPKDFTVIGVN
KYRQVAMMANEDRHLYGVQFHPEVTHTKKGKSILERFITCICRCKSSWKIANIIDDIVMDTRVKVGNDKVVLGFSGGIDS
LVTALLLRRAIGHQFICIYIDNGLLSNYEFDRIKNFCALNCNLDIIYLSQEQRFFNALIGVNDPEKKRKIIGRVFTEVFE
EQIRNLVAVKWLAQGTIYSDVIESGVSLSSFKNVIKSHHNVGGFCGITDIQLLEPIRNLFKDEVRSIGLDLGIPYDIAYR
HPFPGPGLAIRILGEVKKEYCDILRRVDFIFIEELKREHLYSKISQAFAVFLPTHSVGIQGDQRKYKWVIALRAVETIDF
MTAHWAYLSYDFLNKVSNRIVNEVEEVSRVVYDISSKPPATIEWE

Specific function: Catalyzes the synthesis of GMP from XMP

COG id: COG0519

COG function: function code F; GMP synthase, PP-ATPase domain/subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GMP-binding domain

Homologues:

Organism=Homo sapiens, GI4504035, Length=567, Percent_Identity=31.2169312169312, Blast_Score=245, Evalue=7e-65,
Organism=Escherichia coli, GI1788854, Length=522, Percent_Identity=59.1954022988506, Blast_Score=667, Evalue=0.0,
Organism=Caenorhabditis elegans, GI71992717, Length=578, Percent_Identity=33.5640138408304, Blast_Score=266, Evalue=2e-71,
Organism=Caenorhabditis elegans, GI71992710, Length=578, Percent_Identity=33.5640138408304, Blast_Score=266, Evalue=2e-71,
Organism=Caenorhabditis elegans, GI133901714, Length=578, Percent_Identity=33.5640138408304, Blast_Score=266, Evalue=2e-71,
Organism=Saccharomyces cerevisiae, GI6323873, Length=526, Percent_Identity=46.0076045627376, Blast_Score=476, Evalue=1e-135,
Organism=Drosophila melanogaster, GI281365319, Length=556, Percent_Identity=32.5539568345324, Blast_Score=261, Evalue=1e-69,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GUAA_BLOPB (Q492E5)

Other databases:

- EMBL:   CP000016
- RefSeq:   YP_278033.1
- ProteinModelPortal:   Q492E5
- SMR:   Q492E5
- STRING:   Q492E5
- GeneID:   3562883
- GenomeReviews:   CP000016_GR
- KEGG:   bpn:BPEN_546
- eggNOG:   COG0519
- HOGENOM:   HBG391775
- OMA:   MKLVEPL
- ProtClustDB:   PRK00074
- BioCyc:   CBLO291272:BPEN_546-MONOMER
- HAMAP:   MF_00344
- InterPro:   IPR006220
- InterPro:   IPR001317
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR001674
- InterPro:   IPR004739
- InterPro:   IPR022955
- InterPro:   IPR022310
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- PRINTS:   PR00097
- PRINTS:   PR00099
- PRINTS:   PR00096
- TIGRFAMs:   TIGR00884
- TIGRFAMs:   TIGR00888

Pfam domain/function: PF00117 GATase; PF00958 GMP_synt_C; PF02540 NAD_synthase

EC number: =6.3.5.2

Molecular weight: Translated: 59965; Mature: 59965

Theoretical pI: Translated: 7.47; Mature: 7.47

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 84-84 ACT_SITE 181-181 ACT_SITE 183-183

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure: 

>Translated Secondary Structure
MKCNNHRILVIDFGSQYTQLLLRRIRELGVYSESCSWNISKSQVYAFNPNGIILSGGPYS
CCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCEEEECCCCEE
VIDNDSPYIPEFVFQLGIPIFGICYGMQIMSFQLGGRVERVIAQREFGCTQVTILSKSVF
EECCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCEEEEEHHHHH
INDIYDYVDDVTGRFALDVWMSHGDVVTTVPKDFTVIGVNKYRQVAMMANEDRHLYGVQF
HHHHHHHHHHCCCCEEEEEEECCCCEEEECCCCEEEEECHHHHHHHEEECCCCEEEEEEE
HPEVTHTKKGKSILERFITCICRCKSSWKIANIIDDIVMDTRVKVGNDKVVLGFSGGIDS
CCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCEEEECCCEEEEEECCCHHH
LVTALLLRRAIGHQFICIYIDNGLLSNYEFDRIKNFCALNCNLDIIYLSQEQRFFNALIG
HHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHCCEEEEECCEEEEEECCHHHHHHHHHC
VNDPEKKRKIIGRVFTEVFEEQIRNLVAVKWLAQGTIYSDVIESGVSLSSFKNVIKSHHN
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHCCC
VGGFCGITDIQLLEPIRNLFKDEVRSIGLDLGIPYDIAYRHPFPGPGLAIRILGEVKKEY
CCCEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCEEHHHHHHHHHH
CDILRRVDFIFIEELKREHLYSKISQAFAVFLPTHSVGIQGDQRKYKWVIALRAVETIDF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCEEEEEEEHHHHHHHH
MTAHWAYLSYDFLNKVSNRIVNEVEEVSRVVYDISSKPPATIEWE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECC
>Mature Secondary Structure
MKCNNHRILVIDFGSQYTQLLLRRIRELGVYSESCSWNISKSQVYAFNPNGIILSGGPYS
CCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCEEEECCCCEE
VIDNDSPYIPEFVFQLGIPIFGICYGMQIMSFQLGGRVERVIAQREFGCTQVTILSKSVF
EECCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCEEEEEHHHHH
INDIYDYVDDVTGRFALDVWMSHGDVVTTVPKDFTVIGVNKYRQVAMMANEDRHLYGVQF
HHHHHHHHHHCCCCEEEEEEECCCCEEEECCCCEEEEECHHHHHHHEEECCCCEEEEEEE
HPEVTHTKKGKSILERFITCICRCKSSWKIANIIDDIVMDTRVKVGNDKVVLGFSGGIDS
CCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCEEEECCCEEEEEECCCHHH
LVTALLLRRAIGHQFICIYIDNGLLSNYEFDRIKNFCALNCNLDIIYLSQEQRFFNALIG
HHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHCCEEEEECCEEEEEECCHHHHHHHHHC
VNDPEKKRKIIGRVFTEVFEEQIRNLVAVKWLAQGTIYSDVIESGVSLSSFKNVIKSHHN
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHCCC
VGGFCGITDIQLLEPIRNLFKDEVRSIGLDLGIPYDIAYRHPFPGPGLAIRILGEVKKEY
CCCEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCEEHHHHHHHHHH
CDILRRVDFIFIEELKREHLYSKISQAFAVFLPTHSVGIQGDQRKYKWVIALRAVETIDF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCEEEEEEEHHHHHHHH
MTAHWAYLSYDFLNKVSNRIVNEVEEVSRVVYDISSKPPATIEWE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA