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The map label for this gene is ptsI [H]

Identifier: 71892280

GI number: 71892280

Start: 629590

End: 631323

Strand: Direct

Name: ptsI [H]

Synonym: BPEN_527

Alternate gene names: 71892280

Gene position: 629590-631323 (Clockwise)

Preceding gene: 71892279

Following gene: 71892285

Centisome position: 79.53

GC content: 32.64

Gene sequence:

>1734_bases
ATGATTTCAGGAATTTCAGTATCACCTGGCATCGCCTTCGGAAAAGCGTTGCTATTACAAGAAAAAAAAATCATTATCAA
TTTAGAAAAAATCAATATTGGTACTATTGAACAAGAAATCAATCGATTTCTTTCCGGGCGTTCCGAAACTTCCAAGCAAT
TGAAAGAAATTAAAAATAAAGTAAAAAAACAGTTAGACTCTAAAAAAGAAGCTATATTTGAAGGCCATATTATATTGCTA
GAAGATGAAGAACTTGAGCAAGATATTATTACTCTTATTAAAGAAGAATTATTCAGCGCTGATGCCGCCGTGCATTCAGT
AATTGAAACCCAAGCAAAAGCTTTAGAGCAACTAGAGGACGAGTATTTAAAAGAACGTGCTACTGATGTACGAGATATTG
GAAATCGTTTATTAAAAAACATTCTAGGAATTCCAATTATCGACTTAAATTCTATTACCGAAGAAGTGATCATTATTGCA
GTAGATCTTAGTCCATCAGAAACTGCACAATTAAATTTAAAAAAAGTTTTGGGATTTATTACTGATGCCGGCGGAAAAAC
TTCACACACCTCTATTATGGCCCGTTCTTTAGAACTACCAGCCATCGTAGGAACTGGTATAATTACAAAAAGAGTATCCA
ATGGAGATTGGATAATACTCGATGCCTTAAATAATAAAATTTACATCAATCCAACCCCAAACATCATCAAAAATATAAAT
ACATTAAAAAAGAAATATATTTCTGAAAAATATGAGTTAACTAAATTAAAGAATCTCCCAGCTATTACTCTCGACGGACG
TCAAGTACGAATATGCGCTAATATCGGTACGATACACGATATAGTTAGAGCTAAAGAAAACGGAGCTGAAGGAATAGGAT
TATATAGAACTGAATTTTTATTTATGAATCGTAATTCATTCCCCACAGAAGAAGAACAATTTAAAGCTTATAAAAATGCC
GCTGAATCCATGATTAACGAATCTGTAGTCATACGTATTATGGATATTGGGGGTGATAAAAATTTACCTTACATGCACCT
CCCAAACGAAGAAAATCCATTTCTTGGATGGCGCGCAATTCGCATTATGTTAGACAGAAAAGACATATTACATACTCAAT
TACGCGCTATCTTACGAGCTTCCTTTTTTGGTAAATTGCGCATAATGTATCCTATGATTATTTCAGTAGAAGAAGTAAAA
ACTTTAAATACAGAATTAAATTTTTTAAAAACACAATTACGTCAAGAAGGAAAAAAATTTGACGAAAATATTAAAGTAGG
TGTCATGATCGAAACTCCTGCTTCTGCAATTATCGCTCATCATTTAGTTAAAGAAGTGGATTTTTTCAGTATTGGAACTA
ACGATTTAACTCAATATACACTTGCAGTAGATCGCGGCAACAAATTAATATCTCATCTATATAATCCCATATCTCCGTCT
ATTCTAATATTAATTAAAAAAGTAATCGAAGCTTCACATACTGTAGGGAAATGGACAGCTATGTGCGGAGAAATGGCAGG
AGATGAACGTACTACTCTACTATTACTGGGAATGGGATTAGATGAATTTAGCATGAGCTCTATATCTATTCCACACATAA
AAAACATAATCCGTAACGCATATTATCGCGATGCAAAAAAATTGGCACAAAAAGCATTGAACTATTCAACCACAGAATCA
TTAATGCATTTACTTACACATACACATGTGTCACATAATATACAAAAATTATAA

Upstream 100 bases:

>100_bases
AACATTTAATTGAGTTTATTACTGAACTCAAATAAATACAATATAATCAATTAATATTTGAATAATTACACTATTTATTA
ACTCTAATGAAGGTAGAATT

Downstream 100 bases:

>100_bases
AACTATTCTTTTTTTAAAACACCACTATGATAGTAAACATATAATTTAAGAATAAAAATTTTTTATAATTTTATAAATAA
ATCATCGTATAGTTTATTTG

Product: phosphoenolpyruvate-protein phosphotransferase

Products: NA

Alternate protein names: Phosphotransferase system, enzyme I [H]

Number of amino acids: Translated: 577; Mature: 577

Protein sequence:

>577_residues
MISGISVSPGIAFGKALLLQEKKIIINLEKINIGTIEQEINRFLSGRSETSKQLKEIKNKVKKQLDSKKEAIFEGHIILL
EDEELEQDIITLIKEELFSADAAVHSVIETQAKALEQLEDEYLKERATDVRDIGNRLLKNILGIPIIDLNSITEEVIIIA
VDLSPSETAQLNLKKVLGFITDAGGKTSHTSIMARSLELPAIVGTGIITKRVSNGDWIILDALNNKIYINPTPNIIKNIN
TLKKKYISEKYELTKLKNLPAITLDGRQVRICANIGTIHDIVRAKENGAEGIGLYRTEFLFMNRNSFPTEEEQFKAYKNA
AESMINESVVIRIMDIGGDKNLPYMHLPNEENPFLGWRAIRIMLDRKDILHTQLRAILRASFFGKLRIMYPMIISVEEVK
TLNTELNFLKTQLRQEGKKFDENIKVGVMIETPASAIIAHHLVKEVDFFSIGTNDLTQYTLAVDRGNKLISHLYNPISPS
ILILIKKVIEASHTVGKWTAMCGEMAGDERTTLLLLGMGLDEFSMSSISIPHIKNIIRNAYYRDAKKLAQKALNYSTTES
LMHLLTHTHVSHNIQKL

Sequences:

>Translated_577_residues
MISGISVSPGIAFGKALLLQEKKIIINLEKINIGTIEQEINRFLSGRSETSKQLKEIKNKVKKQLDSKKEAIFEGHIILL
EDEELEQDIITLIKEELFSADAAVHSVIETQAKALEQLEDEYLKERATDVRDIGNRLLKNILGIPIIDLNSITEEVIIIA
VDLSPSETAQLNLKKVLGFITDAGGKTSHTSIMARSLELPAIVGTGIITKRVSNGDWIILDALNNKIYINPTPNIIKNIN
TLKKKYISEKYELTKLKNLPAITLDGRQVRICANIGTIHDIVRAKENGAEGIGLYRTEFLFMNRNSFPTEEEQFKAYKNA
AESMINESVVIRIMDIGGDKNLPYMHLPNEENPFLGWRAIRIMLDRKDILHTQLRAILRASFFGKLRIMYPMIISVEEVK
TLNTELNFLKTQLRQEGKKFDENIKVGVMIETPASAIIAHHLVKEVDFFSIGTNDLTQYTLAVDRGNKLISHLYNPISPS
ILILIKKVIEASHTVGKWTAMCGEMAGDERTTLLLLGMGLDEFSMSSISIPHIKNIIRNAYYRDAKKLAQKALNYSTTES
LMHLLTHTHVSHNIQKL
>Mature_577_residues
MISGISVSPGIAFGKALLLQEKKIIINLEKINIGTIEQEINRFLSGRSETSKQLKEIKNKVKKQLDSKKEAIFEGHIILL
EDEELEQDIITLIKEELFSADAAVHSVIETQAKALEQLEDEYLKERATDVRDIGNRLLKNILGIPIIDLNSITEEVIIIA
VDLSPSETAQLNLKKVLGFITDAGGKTSHTSIMARSLELPAIVGTGIITKRVSNGDWIILDALNNKIYINPTPNIIKNIN
TLKKKYISEKYELTKLKNLPAITLDGRQVRICANIGTIHDIVRAKENGAEGIGLYRTEFLFMNRNSFPTEEEQFKAYKNA
AESMINESVVIRIMDIGGDKNLPYMHLPNEENPFLGWRAIRIMLDRKDILHTQLRAILRASFFGKLRIMYPMIISVEEVK
TLNTELNFLKTQLRQEGKKFDENIKVGVMIETPASAIIAHHLVKEVDFFSIGTNDLTQYTLAVDRGNKLISHLYNPISPS
ILILIKKVIEASHTVGKWTAMCGEMAGDERTTLLLLGMGLDEFSMSSISIPHIKNIIRNAYYRDAKKLAQKALNYSTTES
LMHLLTHTHVSHNIQKL

Specific function: General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their tr

COG id: COG1080

COG function: function code G; Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=565, Percent_Identity=68.4955752212389, Blast_Score=801, Evalue=0.0,
Organism=Escherichia coli, GI1788726, Length=526, Percent_Identity=37.0722433460076, Blast_Score=355, Evalue=4e-99,
Organism=Escherichia coli, GI48994992, Length=511, Percent_Identity=37.1819960861057, Blast_Score=322, Evalue=3e-89,
Organism=Escherichia coli, GI1789193, Length=583, Percent_Identity=32.2469982847341, Blast_Score=298, Evalue=5e-82,
Organism=Escherichia coli, GI1787994, Length=449, Percent_Identity=24.4988864142539, Blast_Score=123, Evalue=3e-29,
Organism=Escherichia coli, GI226510935, Length=193, Percent_Identity=29.0155440414508, Blast_Score=72, Evalue=1e-13,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 64971; Mature: 64971

Theoretical pI: Translated: 8.22; Mature: 8.22

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISGISVSPGIAFGKALLLQEKKIIINLEKINIGTIEQEINRFLSGRSETSKQLKEIKNK
CCCCCCCCCCHHHHHHHHEECCEEEEEEEEECCCHHHHHHHHHHCCCCHHHHHHHHHHHH
VKKQLDSKKEAIFEGHIILLEDEELEQDIITLIKEELFSADAAVHSVIETQAKALEQLED
HHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EYLKERATDVRDIGNRLLKNILGIPIIDLNSITEEVIIIAVDLSPSETAQLNLKKVLGFI
HHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHCCEEEEEEEECCCCCHHHHHHHHHHHHH
TDAGGKTSHTSIMARSLELPAIVGTGIITKRVSNGDWIILDALNNKIYINPTPNIIKNIN
HCCCCCCCHHHHHHHHCCCCHHHCCCHHEEEECCCCEEEEEECCCEEEECCCCHHHHHHH
TLKKKYISEKYELTKLKNLPAITLDGRQVRICANIGTIHDIVRAKENGAEGIGLYRTEFL
HHHHHHHHHHHHHHHHCCCCEEEECCCEEEEEECCCHHHHHHHHHCCCCCCCCEEEEEEE
FMNRNSFPTEEEQFKAYKNAAESMINESVVIRIMDIGGDKNLPYMHLPNEENPFLGWRAI
EEECCCCCCHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCEEECCCCCCCCCHHEEE
RIMLDRKDILHTQLRAILRASFFGKLRIMYPMIISVEEVKTLNTELNFLKTQLRQEGKKF
EEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
DENIKVGVMIETPASAIIAHHLVKEVDFFSIGTNDLTQYTLAVDRGNKLISHLYNPISPS
CCCCEEEEEEECCHHHHHHHHHHHHCCEEECCCCCHHEEEEEECCCHHHHHHHCCCCCHH
ILILIKKVIEASHTVGKWTAMCGEMAGDERTTLLLLGMGLDEFSMSSISIPHIKNIIRNA
HHHHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEEEEECCCCHHCCCCCCCHHHHHHHHHH
YYRDAKKLAQKALNYSTTESLMHLLTHTHVSHNIQKL
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MISGISVSPGIAFGKALLLQEKKIIINLEKINIGTIEQEINRFLSGRSETSKQLKEIKNK
CCCCCCCCCCHHHHHHHHEECCEEEEEEEEECCCHHHHHHHHHHCCCCHHHHHHHHHHHH
VKKQLDSKKEAIFEGHIILLEDEELEQDIITLIKEELFSADAAVHSVIETQAKALEQLED
HHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EYLKERATDVRDIGNRLLKNILGIPIIDLNSITEEVIIIAVDLSPSETAQLNLKKVLGFI
HHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHCCEEEEEEEECCCCCHHHHHHHHHHHHH
TDAGGKTSHTSIMARSLELPAIVGTGIITKRVSNGDWIILDALNNKIYINPTPNIIKNIN
HCCCCCCCHHHHHHHHCCCCHHHCCCHHEEEECCCCEEEEEECCCEEEECCCCHHHHHHH
TLKKKYISEKYELTKLKNLPAITLDGRQVRICANIGTIHDIVRAKENGAEGIGLYRTEFL
HHHHHHHHHHHHHHHHCCCCEEEECCCEEEEEECCCHHHHHHHHHCCCCCCCCEEEEEEE
FMNRNSFPTEEEQFKAYKNAAESMINESVVIRIMDIGGDKNLPYMHLPNEENPFLGWRAI
EEECCCCCCHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCEEECCCCCCCCCHHEEE
RIMLDRKDILHTQLRAILRASFFGKLRIMYPMIISVEEVKTLNTELNFLKTQLRQEGKKF
EEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
DENIKVGVMIETPASAIIAHHLVKEVDFFSIGTNDLTQYTLAVDRGNKLISHLYNPISPS
CCCCEEEEEEECCHHHHHHHHHHHHCCEEECCCCCHHEEEEEECCCHHHHHHHCCCCCHH
ILILIKKVIEASHTVGKWTAMCGEMAGDERTTLLLLGMGLDEFSMSSISIPHIKNIIRNA
HHHHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEEEEECCCCHHCCCCCCCHHHHHHHHHH
YYRDAKKLAQKALNYSTTESLMHLLTHTHVSHNIQKL
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2457575; 2960675; 9205837; 9278503; 2411636; 3290198; 9298646; 7876255; 8805571; 9054557; 9541412; 10048929 [H]