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The map label for this gene is folC [H]

Identifier: 71892267

GI number: 71892267

Start: 610496

End: 611770

Strand: Reverse

Name: folC [H]

Synonym: BPEN_510

Alternate gene names: 71892267

Gene position: 611770-610496 (Counterclockwise)

Preceding gene: 71892268

Following gene: 71892266

Centisome position: 77.28

GC content: 33.18

Gene sequence:

>1275_bases
ATGAATAAAATTTTTCATACTCCCCCCGAGCGATCATCTCTGGGAAAATGGCTGAATTATATTGAATCTTTAAGTCATGT
ATTAATTGATTTAAATCTCGATAGGGTACGAAAAATAGCTATTAATCTTGGTTTAATGCATCCTGCAGAGTATGTTATAC
TAGTTGGAGGTACAAATGGTAAGGGCACCACATGTTGTTTATTAGAAACTATTTTATTAAATAATAATAATACGGTTGGT
TTATATACTTCTCCTCATCTACTTTCTTATAATGAACGGATTCGAGTGTGTGGAAAAGAGCTTCCAGATAGTATTCATAT
TGAAGCATTGTCTGTTATAGAGTCCGTGCGTCATCATATTGCATTAACTTATTTTGAATTTATTACTTTGTCTGCTTTGT
ATATATTTAAGCGAGCTAAATTAGATGTAGTAATTCTTGAAGTTGGATTAGGCGGTAGATTAGATGCAACAAATATTGTC
AACCCTGATATTTCTGTAATTACTAATTTTGCAATAGATCATACTGATTTTTTAGGTATAAATCGTGATATGATTGCACA
GGAAAAATCAGGTATTTTTCGTTTTAACAAACCAGCAGTAATTGGTGAAAGTTGTTTTCCAGTGATTGCGCGCAAAATGG
CACAATATTGTGGAGCAATGTTGTTTGCGCGTGGGAGGGACTGGAATTTTCGTGTTTATGGAAATAAGTGGATTTGGTGG
GAGTGCTCTAATGAGTATAAATTATTATATGATTTACCGTTACCTATAATTCCAATAGAAAATGCCGCTACAGCGTTAAG
TGTATTATATTGGTTACCTTTTTTGGTGGATAAAACAGCAATAAGCTATGGGTTGCAAGTAGCTACGTTGCCAGGACGTT
TTCAAATAGTTACTCGCAGACCAATTGCAATATTAGATGTAGGGCATAATCCTCATGCTGCTAATTATTTAACTAAACGA
TTACCTGTCCTTCTTAAAAGAAGGACAGGTAAAGTGAGAATGGTCATAGGAATGTTAAAAAATAAGGATATTCAAGGTAC
CATATATTGTTTAAGTCATTTGGTTGACGTCTGGTATTGTTCTGATTTAAATGTCCCGTTTTCTGCAAGTTCTGTAGAAT
TATCTGATTGTTTGTTGCATTGTAATGTTCAAATATTTAGTAATATATTAGATGCTTGGAATCAAGCTGTATTAGATGCA
TCATTTGATGATTGCATTTTAGTATTTGGATCTTTTCATGCTGTCAGTCCAATTATGAGAAATATAATGAAGTAG

Upstream 100 bases:

>100_bases
ACCAAAAGTTGATTATAGACATTGTGTTGAATAGATATCTATATTTTTATTAATTTATATCCATGAAAAAATACTACAAT
AATTAAAATTGTTGTAAAAT

Downstream 100 bases:

>100_bases
TTATAAATTTATATTTTTGTGTATATTCTGATCAGTGTTAGGAATATGATGGTCTTTGATATTTTTGTTATTAAATAGTT
TACATTAATTATTTAATATT

Product: dihydrofolate synthase

Products: NA

Alternate protein names: Folylpolyglutamate synthase; Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolylpolyglutamate synthase; Tetrahydrofolate synthase; Dihydrofolate synthase [H]

Number of amino acids: Translated: 424; Mature: 424

Protein sequence:

>424_residues
MNKIFHTPPERSSLGKWLNYIESLSHVLIDLNLDRVRKIAINLGLMHPAEYVILVGGTNGKGTTCCLLETILLNNNNTVG
LYTSPHLLSYNERIRVCGKELPDSIHIEALSVIESVRHHIALTYFEFITLSALYIFKRAKLDVVILEVGLGGRLDATNIV
NPDISVITNFAIDHTDFLGINRDMIAQEKSGIFRFNKPAVIGESCFPVIARKMAQYCGAMLFARGRDWNFRVYGNKWIWW
ECSNEYKLLYDLPLPIIPIENAATALSVLYWLPFLVDKTAISYGLQVATLPGRFQIVTRRPIAILDVGHNPHAANYLTKR
LPVLLKRRTGKVRMVIGMLKNKDIQGTIYCLSHLVDVWYCSDLNVPFSASSVELSDCLLHCNVQIFSNILDAWNQAVLDA
SFDDCILVFGSFHAVSPIMRNIMK

Sequences:

>Translated_424_residues
MNKIFHTPPERSSLGKWLNYIESLSHVLIDLNLDRVRKIAINLGLMHPAEYVILVGGTNGKGTTCCLLETILLNNNNTVG
LYTSPHLLSYNERIRVCGKELPDSIHIEALSVIESVRHHIALTYFEFITLSALYIFKRAKLDVVILEVGLGGRLDATNIV
NPDISVITNFAIDHTDFLGINRDMIAQEKSGIFRFNKPAVIGESCFPVIARKMAQYCGAMLFARGRDWNFRVYGNKWIWW
ECSNEYKLLYDLPLPIIPIENAATALSVLYWLPFLVDKTAISYGLQVATLPGRFQIVTRRPIAILDVGHNPHAANYLTKR
LPVLLKRRTGKVRMVIGMLKNKDIQGTIYCLSHLVDVWYCSDLNVPFSASSVELSDCLLHCNVQIFSNILDAWNQAVLDA
SFDDCILVFGSFHAVSPIMRNIMK
>Mature_424_residues
MNKIFHTPPERSSLGKWLNYIESLSHVLIDLNLDRVRKIAINLGLMHPAEYVILVGGTNGKGTTCCLLETILLNNNNTVG
LYTSPHLLSYNERIRVCGKELPDSIHIEALSVIESVRHHIALTYFEFITLSALYIFKRAKLDVVILEVGLGGRLDATNIV
NPDISVITNFAIDHTDFLGINRDMIAQEKSGIFRFNKPAVIGESCFPVIARKMAQYCGAMLFARGRDWNFRVYGNKWIWW
ECSNEYKLLYDLPLPIIPIENAATALSVLYWLPFLVDKTAISYGLQVATLPGRFQIVTRRPIAILDVGHNPHAANYLTKR
LPVLLKRRTGKVRMVIGMLKNKDIQGTIYCLSHLVDVWYCSDLNVPFSASSVELSDCLLHCNVQIFSNILDAWNQAVLDA
SFDDCILVFGSFHAVSPIMRNIMK

Specific function: Conversion of folates to polyglutamate derivatives [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932984, Length=156, Percent_Identity=35.8974358974359, Blast_Score=105, Evalue=8e-23,
Organism=Homo sapiens, GI66932990, Length=156, Percent_Identity=35.8974358974359, Blast_Score=105, Evalue=9e-23,
Organism=Escherichia coli, GI1788654, Length=419, Percent_Identity=50.1193317422434, Blast_Score=399, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI17553150, Length=416, Percent_Identity=27.4038461538462, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI17553148, Length=417, Percent_Identity=27.3381294964029, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI71984923, Length=417, Percent_Identity=27.3381294964029, Blast_Score=119, Evalue=3e-27,
Organism=Saccharomyces cerevisiae, GI6323760, Length=316, Percent_Identity=29.746835443038, Blast_Score=111, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6324815, Length=174, Percent_Identity=33.9080459770115, Blast_Score=90, Evalue=7e-19,
Organism=Drosophila melanogaster, GI24641571, Length=241, Percent_Identity=31.9502074688797, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24581568, Length=179, Percent_Identity=34.6368715083799, Blast_Score=83, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17; =6.3.2.12 [H]

Molecular weight: Translated: 47840; Mature: 47840

Theoretical pI: Translated: 8.21; Mature: 8.21

Prosite motif: PS01012 FOLYLPOLYGLU_SYNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKIFHTPPERSSLGKWLNYIESLSHVLIDLNLDRVRKIAINLGLMHPAEYVILVGGTNG
CCCCCCCCCCHHHHHHHHHHHHHHHHEEEECCHHHHHHHHHHCCCCCCCCEEEEEECCCC
KGTTCCLLETILLNNNNTVGLYTSPHLLSYNERIRVCGKELPDSIHIEALSVIESVRHHI
CCCHHHEEEEHEECCCCEEEEEECCCEECCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
ALTYFEFITLSALYIFKRAKLDVVILEVGLGGRLDATNIVNPDISVITNFAIDHTDFLGI
HHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCCCCCCCCCCCCHHHHEEEEECCCCEECC
NRDMIAQEKSGIFRFNKPAVIGESCFPVIARKMAQYCGAMLFARGRDWNFRVYGNKWIWW
CHHHHHHHCCCCEEECCCCEECCCHHHHHHHHHHHHHHHHHEECCCCCEEEEECCEEEEE
ECSNEYKLLYDLPLPIIPIENAATALSVLYWLPFLVDKTAISYGLQVATLPGRFQIVTRR
EECCCEEEEEECCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCEEEEEEEC
PIAILDVGHNPHAANYLTKRLPVLLKRRTGKVRMVIGMLKNKDIQGTIYCLSHLVDVWYC
CEEEEECCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEHHCCCCCCHHHHHHHHHHHHEEE
SDLNVPFSASSVELSDCLLHCNVQIFSNILDAWNQAVLDASFDDCILVFGSFHAVSPIMR
CCCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHH
NIMK
HHCC
>Mature Secondary Structure
MNKIFHTPPERSSLGKWLNYIESLSHVLIDLNLDRVRKIAINLGLMHPAEYVILVGGTNG
CCCCCCCCCCHHHHHHHHHHHHHHHHEEEECCHHHHHHHHHHCCCCCCCCEEEEEECCCC
KGTTCCLLETILLNNNNTVGLYTSPHLLSYNERIRVCGKELPDSIHIEALSVIESVRHHI
CCCHHHEEEEHEECCCCEEEEEECCCEECCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
ALTYFEFITLSALYIFKRAKLDVVILEVGLGGRLDATNIVNPDISVITNFAIDHTDFLGI
HHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCCCCCCCCCCCCHHHHEEEEECCCCEECC
NRDMIAQEKSGIFRFNKPAVIGESCFPVIARKMAQYCGAMLFARGRDWNFRVYGNKWIWW
CHHHHHHHCCCCEEECCCCEECCCHHHHHHHHHHHHHHHHHEECCCCCEEEEECCEEEEE
ECSNEYKLLYDLPLPIIPIENAATALSVLYWLPFLVDKTAISYGLQVATLPGRFQIVTRR
EECCCEEEEEECCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCEEEEEEEC
PIAILDVGHNPHAANYLTKRLPVLLKRRTGKVRMVIGMLKNKDIQGTIYCLSHLVDVWYC
CEEEEECCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEHHCCCCCCHHHHHHHHHHHHEEE
SDLNVPFSASSVELSDCLLHCNVQIFSNILDAWNQAVLDASFDDCILVFGSFHAVSPIMR
CCCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHH
NIMK
HHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3040739; 3040734; 9205837; 9278503; 1989505 [H]