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The map label for this gene is lpxL [H]

Identifier: 71892190

GI number: 71892190

Start: 501333

End: 502256

Strand: Direct

Name: lpxL [H]

Synonym: BPEN_423

Alternate gene names: 71892190

Gene position: 501333-502256 (Clockwise)

Preceding gene: 71892189

Following gene: 71892403

Centisome position: 63.33

GC content: 34.2

Gene sequence:

>924_bases
ATGAGAAAACTTCCGGTTTTTAATTACTCTTTCTTAAGCCCACGATTTTGGTTAATCTGGATAGGTATAGGAACATTATA
TATTTTAGTATTGCTTCCTTATCCTGTCATACATTATCTTGGTACTAGAATTGGTCGTATTACAAAATATTTTATGACAA
ACCGCATGCGCATTATTCGTAGAAACTTAGAACTCTGTTTTCCTAATTTACCAAGTCAAGAACAAAAAAATTTGTTCAAC
AAAAATTGTGAATCGATAGGTATGGGGGTTTTTGAAACTGGCATGGCTTGGTTTTGGTCAGAACATAGAATTAAACGCTG
GTTTAAAATTAATGGACTAGACAATATAACCAAAGCACGAAAAAAAAATCAAGGCATATTACTAATTGGGATGCATTTTT
TTACTTTAGAATTAGGTGCACGAATTTTAGGTATGCTAAATCCAGGCATTGGTGTCTACCGTCCTAATAATAATCCATTA
TTAAACTGGTTACAAACCAAGGGACGATTAAGATCGAATAAAACCATGTTGGACCGTACTAATATTAAAGGCATGATCAA
AGCTTTGCAACACGGTGAAATTGTCTGGTATGCACCAGATCATGATTATGGATATAAAAACAGTGTATTTACCCCCTTAT
TCGCAGTGTCAAATGCAGCGAGTACTATCGGTACTTATATACTCACAAAAATAACCAAACCTGCAATTGTTCCTTTTGTG
GCACGCAGATTACCTAATCTTCTCGGATATGAACTACTAATTTTGCCAAATCAACAAGATGAAATACCTTTAGACACAAA
TATCAACATGATCAAACACATTAATAAAGTAATCGAACAAGTAATTTTACTAGCCCCTGACCAATATATGTGGTTGCATC
GTCGATTTAAAACCCGTCCTCCTGGAGAATCTCCTTTATATTAA

Upstream 100 bases:

>100_bases
TGAAAACAGATTTCTAAAAATATAGATGATCGTAACAATTAATATTGCAAATATACGCTAATATATTTTTATTATGCCGT
TTATATGATGTCAACTACAT

Downstream 100 bases:

>100_bases
TTAAACAAAAAATCAAACAACTTATTACAGGCCGATAAAATTACTCATATTATATAAATTATTATTGGTATACATGCAGA
TACTAAAAATATGCGTAATT

Product: lipid A biosynthesis lauroyl acyltransferase

Products: NA

Alternate protein names: Heat shock protein B [H]

Number of amino acids: Translated: 307; Mature: 307

Protein sequence:

>307_residues
MRKLPVFNYSFLSPRFWLIWIGIGTLYILVLLPYPVIHYLGTRIGRITKYFMTNRMRIIRRNLELCFPNLPSQEQKNLFN
KNCESIGMGVFETGMAWFWSEHRIKRWFKINGLDNITKARKKNQGILLIGMHFFTLELGARILGMLNPGIGVYRPNNNPL
LNWLQTKGRLRSNKTMLDRTNIKGMIKALQHGEIVWYAPDHDYGYKNSVFTPLFAVSNAASTIGTYILTKITKPAIVPFV
ARRLPNLLGYELLILPNQQDEIPLDTNINMIKHINKVIEQVILLAPDQYMWLHRRFKTRPPGESPLY

Sequences:

>Translated_307_residues
MRKLPVFNYSFLSPRFWLIWIGIGTLYILVLLPYPVIHYLGTRIGRITKYFMTNRMRIIRRNLELCFPNLPSQEQKNLFN
KNCESIGMGVFETGMAWFWSEHRIKRWFKINGLDNITKARKKNQGILLIGMHFFTLELGARILGMLNPGIGVYRPNNNPL
LNWLQTKGRLRSNKTMLDRTNIKGMIKALQHGEIVWYAPDHDYGYKNSVFTPLFAVSNAASTIGTYILTKITKPAIVPFV
ARRLPNLLGYELLILPNQQDEIPLDTNINMIKHINKVIEQVILLAPDQYMWLHRRFKTRPPGESPLY
>Mature_307_residues
MRKLPVFNYSFLSPRFWLIWIGIGTLYILVLLPYPVIHYLGTRIGRITKYFMTNRMRIIRRNLELCFPNLPSQEQKNLFN
KNCESIGMGVFETGMAWFWSEHRIKRWFKINGLDNITKARKKNQGILLIGMHFFTLELGARILGMLNPGIGVYRPNNNPL
LNWLQTKGRLRSNKTMLDRTNIKGMIKALQHGEIVWYAPDHDYGYKNSVFTPLFAVSNAASTIGTYILTKITKPAIVPFV
ARRLPNLLGYELLILPNQQDEIPLDTNINMIKHINKVIEQVILLAPDQYMWLHRRFKTRPPGESPLY

Specific function: Acylates the intermediate (KDO)2-lipid IVA to form (KDO)2-(lauroyl)-lipid IVA. Has 10 fold selectivity for lauroyl- ACP over myristoyl-ACP [H]

COG id: COG1560

COG function: function code M; Lauroyl/myristoyl acyltransferase

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the htrB/msbB family [H]

Homologues:

Organism=Escherichia coli, GI1787292, Length=307, Percent_Identity=56.0260586319218, Blast_Score=377, Evalue=1e-106,
Organism=Escherichia coli, GI87082094, Length=301, Percent_Identity=48.1727574750831, Blast_Score=337, Evalue=7e-94,
Organism=Escherichia coli, GI1788161, Length=307, Percent_Identity=24.1042345276873, Blast_Score=100, Evalue=9e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004960
- InterPro:   IPR011920 [H]

Pfam domain/function: PF03279 Lip_A_acyltrans [H]

EC number: 2.3.1.-

Molecular weight: Translated: 35789; Mature: 35789

Theoretical pI: Translated: 10.69; Mature: 10.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKLPVFNYSFLSPRFWLIWIGIGTLYILVLLPYPVIHYLGTRIGRITKYFMTNRMRIIR
CCCCCCCCCHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RNLELCFPNLPSQEQKNLFNKNCESIGMGVFETGMAWFWSEHRIKRWFKINGLDNITKAR
HHHHHHCCCCCCHHHHHHHHCCHHHHCCCHHHHHHHHHHHHHHHHHHEEECCCHHHHHHH
KKNQGILLIGMHFFTLELGARILGMLNPGIGVYRPNNNPLLNWLQTKGRLRSNKTMLDRT
HCCCCEEEEEHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHCCHHHCCCCHHHHH
NIKGMIKALQHGEIVWYAPDHDYGYKNSVFTPLFAVSNAASTIGTYILTKITKPAIVPFV
HHHHHHHHHHCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
ARRLPNLLGYELLILPNQQDEIPLDTNINMIKHINKVIEQVILLAPDQYMWLHRRFKTRP
HHHCHHHHCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCC
PGESPLY
CCCCCCC
>Mature Secondary Structure
MRKLPVFNYSFLSPRFWLIWIGIGTLYILVLLPYPVIHYLGTRIGRITKYFMTNRMRIIR
CCCCCCCCCHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RNLELCFPNLPSQEQKNLFNKNCESIGMGVFETGMAWFWSEHRIKRWFKINGLDNITKAR
HHHHHHCCCCCCHHHHHHHHCCHHHHCCCHHHHHHHHHHHHHHHHHHEEECCCHHHHHHH
KKNQGILLIGMHFFTLELGARILGMLNPGIGVYRPNNNPLLNWLQTKGRLRSNKTMLDRT
HCCCCEEEEEHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHCCHHHCCCCHHHHH
NIKGMIKALQHGEIVWYAPDHDYGYKNSVFTPLFAVSNAASTIGTYILTKITKPAIVPFV
HHHHHHHHHHCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
ARRLPNLLGYELLILPNQQDEIPLDTNINMIKHINKVIEQVILLAPDQYMWLHRRFKTRP
HHHCHHHHCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCC
PGESPLY
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Acyltransferases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]