Click here to switch to the map view.

The map label for this gene is emtA [H]

Identifier: 71892169

GI number: 71892169

Start: 477175

End: 477819

Strand: Reverse

Name: emtA [H]

Synonym: BPEN_402

Alternate gene names: 71892169

Gene position: 477819-477175 (Counterclockwise)

Preceding gene: 71892170

Following gene: 71892167

Centisome position: 60.36

GC content: 29.61

Gene sequence:

>645_bases
ATGAAGATATACATAATACATTTAGTTGTAATTTTGTTACTAACAACTTGTGCTCAAAAAAATATTGAAAAAAATACTAC
ATGGACATTATATACTAATTCAAAGTACAAGATAATTTATTTTTCTAAACGAATTACACCGTCTATGTATAATGATTACA
TTCATTATTGTGCTATGAATTATGGAGTAGATGCATCTTTAGTGAAAGCCATTATTCAAGTAGAATCTAATTATAATCCT
ACTGTAATTAGTAAATCTAATGCTGTTGGATTAATGCAGCTTAAAGCGGATACTGCAGGAAGAGATGCTTATCGTTTGAA
AGGATGGAGAGGAGAACCTAGTATTCATGAATTAAAAAATGCTGCTGTTAATATTGATCTTGGTACTGCTTATTTGTCTA
TATTGCAAAAACAATTAGAGGGAATTATTGATGTAAAAACTAGACGATACGCCATAATTGTTGCTTACGTGAATGGTTTA
AGTGCGTTGTTGAAAACATTTTCTATTGACCGTAATTACGCCCTTGAAAAAATAAACAAACTTAATCCGGAACAATTTTA
CCAACATATACAATCTCATCATCCATCTAAGCAAGCGCAACGCTATTTATTTAAAGTTAATTCTGTTTATGTTACACAAA
ATTAG

Upstream 100 bases:

>100_bases
AAAATTGTGGAAAAATTACTTTTTTAAAGTATGAGAGCATCACTTTTATATAATAATTTTATGAAGAAATACATATTTTG
ATCATAAAAATCATGAAATT

Downstream 100 bases:

>100_bases
TTGTAATAATAGTTTTTTATTTTTATAAAAATATTTTATTTTTGATAAATTTTATGAGGTATACGTTATAGCTATTGTGA
TTTGGTGGAACATATTATTA

Product: membrane-bound lytic murein transglycosylase E

Products: NA

Alternate protein names: Peptidoglycan lytic endotransglycosylase [H]

Number of amino acids: Translated: 214; Mature: 214

Protein sequence:

>214_residues
MKIYIIHLVVILLLTTCAQKNIEKNTTWTLYTNSKYKIIYFSKRITPSMYNDYIHYCAMNYGVDASLVKAIIQVESNYNP
TVISKSNAVGLMQLKADTAGRDAYRLKGWRGEPSIHELKNAAVNIDLGTAYLSILQKQLEGIIDVKTRRYAIIVAYVNGL
SALLKTFSIDRNYALEKINKLNPEQFYQHIQSHHPSKQAQRYLFKVNSVYVTQN

Sequences:

>Translated_214_residues
MKIYIIHLVVILLLTTCAQKNIEKNTTWTLYTNSKYKIIYFSKRITPSMYNDYIHYCAMNYGVDASLVKAIIQVESNYNP
TVISKSNAVGLMQLKADTAGRDAYRLKGWRGEPSIHELKNAAVNIDLGTAYLSILQKQLEGIIDVKTRRYAIIVAYVNGL
SALLKTFSIDRNYALEKINKLNPEQFYQHIQSHHPSKQAQRYLFKVNSVYVTQN
>Mature_214_residues
MKIYIIHLVVILLLTTCAQKNIEKNTTWTLYTNSKYKIIYFSKRITPSMYNDYIHYCAMNYGVDASLVKAIIQVESNYNP
TVISKSNAVGLMQLKADTAGRDAYRLKGWRGEPSIHELKNAAVNIDLGTAYLSILQKQLEGIIDVKTRRYAIIVAYVNGL
SALLKTFSIDRNYALEKINKLNPEQFYQHIQSHHPSKQAQRYLFKVNSVYVTQN

Specific function: Murein-degrading enzyme. May play a role in recycling of muropeptides during cell elongation and/or cell division. Preferentially cleaves at a distance of more than two disaccharide units from the ends of the glycan chain [H]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transglycosylase slt family [H]

Homologues:

Organism=Escherichia coli, GI87081855, Length=198, Percent_Identity=44.9494949494949, Blast_Score=174, Evalue=3e-45,
Organism=Escherichia coli, GI87082191, Length=157, Percent_Identity=44.5859872611465, Blast_Score=139, Evalue=1e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008258
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 24562; Mature: 24562

Theoretical pI: Translated: 9.94; Mature: 9.94

Prosite motif: PS00922 TRANSGLYCOSYLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIYIIHLVVILLLTTCAQKNIEKNTTWTLYTNSKYKIIYFSKRITPSMYNDYIHYCAMN
CEEEHHHHHHHHHHHHHHHCCCCCCCEEEEEECCEEEEEEEECCCCHHHHHHHHHHHHHC
YGVDASLVKAIIQVESNYNPTVISKSNAVGLMQLKADTAGRDAYRLKGWRGEPSIHELKN
CCCCHHHHHHHHHHCCCCCCEEEECCCCEEEEEEECCCCCCCCCEECCCCCCCCHHHHCC
AAVNIDLGTAYLSILQKQLEGIIDVKTRRYAIIVAYVNGLSALLKTFSIDRNYALEKINK
CEEEEEHHHHHHHHHHHHHHHHEEECCCEEEEEEEEHHHHHHHHHHHCCCCHHHHHHHHC
LNPEQFYQHIQSHHPSKQAQRYLFKVNSVYVTQN
CCHHHHHHHHHHCCCHHHHHHHHHEEEEEEEECC
>Mature Secondary Structure
MKIYIIHLVVILLLTTCAQKNIEKNTTWTLYTNSKYKIIYFSKRITPSMYNDYIHYCAMN
CEEEHHHHHHHHHHHHHHHCCCCCCCEEEEEECCEEEEEEEECCCCHHHHHHHHHHHHHC
YGVDASLVKAIIQVESNYNPTVISKSNAVGLMQLKADTAGRDAYRLKGWRGEPSIHELKN
CCCCHHHHHHHHHHCCCCCCEEEECCCCEEEEEEECCCCCCCCCEECCCCCCCCHHHHCC
AAVNIDLGTAYLSILQKQLEGIIDVKTRRYAIIVAYVNGLSALLKTFSIDRNYALEKINK
CEEEEEHHHHHHHHHHHHHHHHEEECCCEEEEEEEEHHHHHHHHHHHCCCCHHHHHHHHC
LNPEQFYQHIQSHHPSKQAQRYLFKVNSVYVTQN
CCHHHHHHHHHHCCCHHHHHHHHHEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA