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The map label for this gene is ybfF [H]

Identifier: 71892103

GI number: 71892103

Start: 394862

End: 395626

Strand: Reverse

Name: ybfF [H]

Synonym: BPEN_334

Alternate gene names: 71892103

Gene position: 395626-394862 (Counterclockwise)

Preceding gene: 71892402

Following gene: 71892102

Centisome position: 49.97

GC content: 34.12

Gene sequence:

>765_bases
ATGCAACTTAATTATCGATTACGAATTCCTAAAACCGTATACCGCAGAACGCCAGTCATAATACTCCATGGACTTTTCGG
GGATCTTAGTAATCTTGGGATTGTTGTAAAAAGCATAGCTCGATATTGTTATGTCGTGCAAGTTGACTTACGTAATCATG
GTCGTTCTCCTCATGAACAATCTATGAATTATTTGGTAATGGCACAAGATATCTTAGATTTGCTAGATCATTTGTTAATA
AACAAATGCATAGTTATTGGTCATTCAATGGGTGGTAAAGTTGCTATGACATTATGTATGTTGGCCCCCCAACGTATAAG
CAAAATCGTGGTTATTGATATAGCTCCAATAAAATATAATCTACATAATCATGATAATATTTTTCATGCAATTGAACGCG
TTAATATATCTGGAGTAAAAAATAGAAACGAAGCTGCACATCTTATGCGGCAGTGTTGTATCGACCAAACATTAATATTA
TTTTTACTAAAATCTTTCCATCAAGGATCTTGGGTTTTTAATTTTTCGTCTATTAGAAATAATTATATTCATATCAGTGA
TTGGAACACATACCAAACTTGGTGGGGTCCAGCATTGTTTATTAGGGGCGCATTATCTTCATATTTTGATGATCGTTATT
TGCACGATGTTTACCATCAATTTCCCCAGGCGCACATTTGCATGATACCTAATGCAGGGCATTGGGTGCATTGGGATAAT
GCTATATATGTATTAAGTAGGATTAATGAATTTATTATCCATTAA

Upstream 100 bases:

>100_bases
AAGTAGATAATCTAATGTATACAACCTACTAAATACACAAAATATTGTTTAGATCGTGATAAACAATATCTCAGTGTTAT
ACTTAATAAGTAATTATGAT

Downstream 100 bases:

>100_bases
TTTTTAAATATATATTTTATTTGGTATTTTTGTAAAAATTTATGTAATCGTAAACAAATAATATTAATGATTAATTAAAA
TTGATTAGCCATGATAATAC

Product: alpha/beta-hydrolase domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 254; Mature: 254

Protein sequence:

>254_residues
MQLNYRLRIPKTVYRRTPVIILHGLFGDLSNLGIVVKSIARYCYVVQVDLRNHGRSPHEQSMNYLVMAQDILDLLDHLLI
NKCIVIGHSMGGKVAMTLCMLAPQRISKIVVIDIAPIKYNLHNHDNIFHAIERVNISGVKNRNEAAHLMRQCCIDQTLIL
FLLKSFHQGSWVFNFSSIRNNYIHISDWNTYQTWWGPALFIRGALSSYFDDRYLHDVYHQFPQAHICMIPNAGHWVHWDN
AIYVLSRINEFIIH

Sequences:

>Translated_254_residues
MQLNYRLRIPKTVYRRTPVIILHGLFGDLSNLGIVVKSIARYCYVVQVDLRNHGRSPHEQSMNYLVMAQDILDLLDHLLI
NKCIVIGHSMGGKVAMTLCMLAPQRISKIVVIDIAPIKYNLHNHDNIFHAIERVNISGVKNRNEAAHLMRQCCIDQTLIL
FLLKSFHQGSWVFNFSSIRNNYIHISDWNTYQTWWGPALFIRGALSSYFDDRYLHDVYHQFPQAHICMIPNAGHWVHWDN
AIYVLSRINEFIIH
>Mature_254_residues
MQLNYRLRIPKTVYRRTPVIILHGLFGDLSNLGIVVKSIARYCYVVQVDLRNHGRSPHEQSMNYLVMAQDILDLLDHLLI
NKCIVIGHSMGGKVAMTLCMLAPQRISKIVVIDIAPIKYNLHNHDNIFHAIERVNISGVKNRNEAAHLMRQCCIDQTLIL
FLLKSFHQGSWVFNFSSIRNNYIHISDWNTYQTWWGPALFIRGALSSYFDDRYLHDVYHQFPQAHICMIPNAGHWVHWDN
AIYVLSRINEFIIH

Specific function: Displays esterase activity toward palmitoyl-CoA, malonyl-CoA and pNP-butyrate [H]

COG id: COG0596

COG function: function code R; Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dmpD/todF/xylF esterase family [H]

Homologues:

Organism=Homo sapiens, GI23200008, Length=247, Percent_Identity=31.9838056680162, Blast_Score=142, Evalue=2e-34,
Organism=Homo sapiens, GI23200012, Length=247, Percent_Identity=31.9838056680162, Blast_Score=142, Evalue=2e-34,
Organism=Homo sapiens, GI223941839, Length=238, Percent_Identity=28.9915966386555, Blast_Score=110, Evalue=9e-25,
Organism=Escherichia coli, GI1786902, Length=252, Percent_Identity=44.4444444444444, Blast_Score=228, Evalue=2e-61,
Organism=Caenorhabditis elegans, GI17508535, Length=255, Percent_Identity=30.1960784313725, Blast_Score=122, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17507063, Length=253, Percent_Identity=26.8774703557312, Blast_Score=90, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6321468, Length=267, Percent_Identity=33.3333333333333, Blast_Score=109, Evalue=5e-25,
Organism=Drosophila melanogaster, GI24640348, Length=257, Percent_Identity=27.6264591439689, Blast_Score=101, Evalue=6e-22,
Organism=Drosophila melanogaster, GI24646055, Length=262, Percent_Identity=23.6641221374046, Blast_Score=85, Evalue=6e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: 3.1.-.- [C]

Molecular weight: Translated: 29603; Mature: 29603

Theoretical pI: Translated: 8.96; Mature: 8.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLNYRLRIPKTVYRRTPVIILHGLFGDLSNLGIVVKSIARYCYVVQVDLRNHGRSPHEQ
CCCCEEEECCHHHHHCCCEEEEECHHCCHHHHHHHHHHHHHHEEEEEEEHHHCCCCCHHH
SMNYLVMAQDILDLLDHLLINKCIVIGHSMGGKVAMTLCMLAPQRISKIVVIDIAPIKYN
CCCEEEHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHCHHHCCEEEEEEEEEEEEE
LHNHDNIFHAIERVNISGVKNRNEAAHLMRQCCIDQTLILFLLKSFHQGSWVFNFSSIRN
CCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHCC
NYIHISDWNTYQTWWGPALFIRGALSSYFDDRYLHDVYHQFPQAHICMIPNAGHWVHWDN
CEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEEECC
AIYVLSRINEFIIH
HHHHHHHHHHHHCC
>Mature Secondary Structure
MQLNYRLRIPKTVYRRTPVIILHGLFGDLSNLGIVVKSIARYCYVVQVDLRNHGRSPHEQ
CCCCEEEECCHHHHHCCCEEEEECHHCCHHHHHHHHHHHHHHEEEEEEEHHHCCCCCHHH
SMNYLVMAQDILDLLDHLLINKCIVIGHSMGGKVAMTLCMLAPQRISKIVVIDIAPIKYN
CCCEEEHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHCHHHCCEEEEEEEEEEEEE
LHNHDNIFHAIERVNISGVKNRNEAAHLMRQCCIDQTLILFLLKSFHQGSWVFNFSSIRN
CCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHCC
NYIHISDWNTYQTWWGPALFIRGALSSYFDDRYLHDVYHQFPQAHICMIPNAGHWVHWDN
CEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEEECC
AIYVLSRINEFIIH
HHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 10493123 [H]