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The map label for this gene is argF [H]

Identifier: 68535920

GI number: 68535920

Start: 1006845

End: 1007786

Strand: Direct

Name: argF [H]

Synonym: jk0845

Alternate gene names: 68535920

Gene position: 1006845-1007786 (Clockwise)

Preceding gene: 68535919

Following gene: 68535921

Centisome position: 40.89

GC content: 63.27

Gene sequence:

>942_bases
ATGACAAACCTGCACCTGCAGAAAAAGGGGCCGCTGCGCCACATGCTGGCCGACGACGACCTCACCCCGGAAGAGCAAGC
CGAAGTGCTGGCGCTGGCCGAGGAACTGAAGGACAACCGCTACGGCAACGGCTACCGCAACCTGCTAGAGCGCCGCTCCG
TCGCCGTGCTGTTCGACAAGACCTCTACGCGCACGCGCTTTTCTTTTGACGCCGGCATCACCGAGTTGGGTGGCAACGCC
ATCGTCGTGGACAGTGGAAGTTCGCAGATGGGGAAGGGAGAGACCTTCCAGGACACCGGCGCGGTCCTGTCCCGCTACGT
CACTGCGATCGTCTGGCGCACCGGCGCGCACGACAACCTCCACCAGATGGCAGAGACCGCGGGCGTACCGATCGTCAACT
CCCTATCCGATGATTTCCACCCCTGCCAGATCCTCGCGGACCTGGTGACCATCAAGGAGCACAAGGGCGAGCTCAAGGGG
TTGAACGCTGTGTACTTCGGCGACGGCGCGAACAACATGGCCAACAGCTACATGTTGGGCTTCGCCACGGCGGGAGTGAA
CATCACTATCGCCGCGCCAGAGGGTTTCCAACCGGAGGCGAAGTTCGTGGAGCGCGCCCAGCAGCGCGCCGAGCTGACCG
GTGCGACTGTGACCGTCACCGACAAGGTGGACGCCACGGGGGCGGACGTGGTGATTACCGACACCTGGCTGTCCATGGGG
CAGGATGCCTCCGAAGACCGCAGCGCGCTGCGGGCCTACCAGGTGGACGAGAAGCTGATGGGCACCGCCAAGAACGATGC
AATCTTCCTGCACTGTCTGCCGGCCTATCGCGGAAGTGAGGTGACGCCGGAGGTCATCGACGGCCCGCAGTCGGTGGTTT
TCGATGAGGCTGAAAACCGCCTGCACGCACAGAAGGCACTGTTGGTATGGCTTCTTCGCTGA

Upstream 100 bases:

>100_bases
TCGGCGGATGAGGTGCAGCGGGGCGTCACAAAGATTAGGCAGATGCTGGACGACAACCGCGCGCAGAACCAGTAACCACA
GAAAGAACGGAAGACACCAC

Downstream 100 bases:

>100_bases
CTCGCACAGCCCGGCAGGACATGATCGGGCGCATCATTGGTGCCGAGAAAGTTGGCAGCCAGCGCGAGCTGCTGGGAATC
CTGGTGAACCGCGGCATTGA

Product: ornithine carbamoyltransferase

Products: NA

Alternate protein names: OTCase [H]

Number of amino acids: Translated: 313; Mature: 312

Protein sequence:

>313_residues
MTNLHLQKKGPLRHMLADDDLTPEEQAEVLALAEELKDNRYGNGYRNLLERRSVAVLFDKTSTRTRFSFDAGITELGGNA
IVVDSGSSQMGKGETFQDTGAVLSRYVTAIVWRTGAHDNLHQMAETAGVPIVNSLSDDFHPCQILADLVTIKEHKGELKG
LNAVYFGDGANNMANSYMLGFATAGVNITIAAPEGFQPEAKFVERAQQRAELTGATVTVTDKVDATGADVVITDTWLSMG
QDASEDRSALRAYQVDEKLMGTAKNDAIFLHCLPAYRGSEVTPEVIDGPQSVVFDEAENRLHAQKALLVWLLR

Sequences:

>Translated_313_residues
MTNLHLQKKGPLRHMLADDDLTPEEQAEVLALAEELKDNRYGNGYRNLLERRSVAVLFDKTSTRTRFSFDAGITELGGNA
IVVDSGSSQMGKGETFQDTGAVLSRYVTAIVWRTGAHDNLHQMAETAGVPIVNSLSDDFHPCQILADLVTIKEHKGELKG
LNAVYFGDGANNMANSYMLGFATAGVNITIAAPEGFQPEAKFVERAQQRAELTGATVTVTDKVDATGADVVITDTWLSMG
QDASEDRSALRAYQVDEKLMGTAKNDAIFLHCLPAYRGSEVTPEVIDGPQSVVFDEAENRLHAQKALLVWLLR
>Mature_312_residues
TNLHLQKKGPLRHMLADDDLTPEEQAEVLALAEELKDNRYGNGYRNLLERRSVAVLFDKTSTRTRFSFDAGITELGGNAI
VVDSGSSQMGKGETFQDTGAVLSRYVTAIVWRTGAHDNLHQMAETAGVPIVNSLSDDFHPCQILADLVTIKEHKGELKGL
NAVYFGDGANNMANSYMLGFATAGVNITIAAPEGFQPEAKFVERAQQRAELTGATVTVTDKVDATGADVVITDTWLSMGQ
DASEDRSALRAYQVDEKLMGTAKNDAIFLHCLPAYRGSEVTPEVIDGPQSVVFDEAENRLHAQKALLVWLLR

Specific function: Arginine biosynthesis; sixth step. [C]

COG id: COG0078

COG function: function code E; Ornithine carbamoyltransferase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family [H]

Homologues:

Organism=Homo sapiens, GI38788445, Length=312, Percent_Identity=39.4230769230769, Blast_Score=205, Evalue=4e-53,
Organism=Homo sapiens, GI18105007, Length=308, Percent_Identity=24.3506493506494, Blast_Score=82, Evalue=8e-16,
Organism=Escherichia coli, GI1790703, Length=323, Percent_Identity=36.2229102167183, Blast_Score=202, Evalue=3e-53,
Organism=Escherichia coli, GI1786469, Length=323, Percent_Identity=35.6037151702786, Blast_Score=200, Evalue=1e-52,
Organism=Escherichia coli, GI2367364, Length=310, Percent_Identity=29.0322580645161, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI48994908, Length=347, Percent_Identity=26.5129682997118, Blast_Score=81, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI193204318, Length=307, Percent_Identity=25.4071661237785, Blast_Score=65, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6322373, Length=312, Percent_Identity=33.6538461538462, Blast_Score=179, Evalue=6e-46,
Organism=Saccharomyces cerevisiae, GI6322331, Length=310, Percent_Identity=28.3870967741935, Blast_Score=100, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24642586, Length=314, Percent_Identity=28.6624203821656, Blast_Score=94, Evalue=8e-20,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2066 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002292 [H]

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N [H]

EC number: =2.1.3.3 [H]

Molecular weight: Translated: 34129; Mature: 33998

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNLHLQKKGPLRHMLADDDLTPEEQAEVLALAEELKDNRYGNGYRNLLERRSVAVLFDK
CCCCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCEEEEEEC
TSTRTRFSFDAGITELGGNAIVVDSGSSQMGKGETFQDTGAVLSRYVTAIVWRTGAHDNL
CCCCEEEEECCCHHHCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHH
HQMAETAGVPIVNSLSDDFHPCQILADLVTIKEHKGELKGLNAVYFGDGANNMANSYMLG
HHHHHHCCCCEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHEEEE
FATAGVNITIAAPEGFQPEAKFVERAQQRAELTGATVTVTDKVDATGADVVITDTWLSMG
EEECCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCEEEEEHHHHCC
QDASEDRSALRAYQVDEKLMGTAKNDAIFLHCLPAYRGSEVTPEVIDGPQSVVFDEAENR
CCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCHHHHCCCHHEEHHHHHHH
LHAQKALLVWLLR
HHHHHHHHHHCCC
>Mature Secondary Structure 
TNLHLQKKGPLRHMLADDDLTPEEQAEVLALAEELKDNRYGNGYRNLLERRSVAVLFDK
CCCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCEEEEEEC
TSTRTRFSFDAGITELGGNAIVVDSGSSQMGKGETFQDTGAVLSRYVTAIVWRTGAHDNL
CCCCEEEEECCCHHHCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHH
HQMAETAGVPIVNSLSDDFHPCQILADLVTIKEHKGELKGLNAVYFGDGANNMANSYMLG
HHHHHHCCCCEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHEEEE
FATAGVNITIAAPEGFQPEAKFVERAQQRAELTGATVTVTDKVDATGADVVITDTWLSMG
EEECCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCEEEEEHHHHCC
QDASEDRSALRAYQVDEKLMGTAKNDAIFLHCLPAYRGSEVTPEVIDGPQSVVFDEAENR
CCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCHHHHCCCHHEEHHHHHHH
LHAQKALLVWLLR
HHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14602910 [H]