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The map label for this gene is pyk [H]

Identifier: 68535876

GI number: 68535876

Start: 954167

End: 955585

Strand: Direct

Name: pyk [H]

Synonym: jk0801

Alternate gene names: 68535876

Gene position: 954167-955585 (Clockwise)

Preceding gene: 68535875

Following gene: 68535879

Centisome position: 38.75

GC content: 65.82

Gene sequence:

>1419_bases
GTGAATAGAAGAACCAAGATTGTTTGCACCCTGGGTCCCGCGGTCGCTTCGAAGGAGCAGATTCGCGGGCTCGTAGACGC
AGGAATGAACGTTGCGCGCCTCAACTTTTCCCACGGCGAGCACGCAGACCACGAGCAGAATTACAACTGGGTTCGACAGG
CCACCGATGAATCCGGCAAGGCCGTCGGTATCCTCGCCGACCTGCAAGGCCCGAAGATCCGCCTCGGACGCTTCGCAGAG
GGCGCAACCGTCTGGGCGACCGGCGAGACCGTACGCATCACCGTCGACGACGTACAGGGCACACACGACCGCGTGTCCAC
CACCTACAAGGGCTTGGCCCGCGACGCCCGCCCCGGCGACCGCCTGCTGGTCGACGACGGCAAGATCGCGCTGGTGTGCA
AGGAAGTCGACGGCAACGACGTCGTGTGCGAGGTCATCGAGGGAGGCCCGGTTTCCAACAACAAGGGTGTCTCCCTGCCG
GGCATGAACATCTCCGTGCCGGCACTGAGCGAGAAGGACACCGAAGACCTGCGCTTCGCCCTGCAGCTGGGCGTGGACTT
CATCGCCCTGTCCTTCGTGCGCAGCCCCTCCGACGTGGAACTGGTGCGCGACATCATGGACGAGGTTGGCCGCCGCGTGC
CGGTGATCGCCAAGCTGGAGAAGCCGGAGGCAGTCGACGCGCTGGAGCCGGTCATCCTAGCCTTCGATGCCATCATGGTC
GCCCGCGGTGACCTGGGCGTGGAAGTGCCGCTGGAAGACGTGCCGCTGGTGCAGAAGCGCGCCATCCAGATCGCCCGCGA
GAACGCCAAGCCGGTGATCGTGGCGACGCAGATGCTGGACTCCATGATCGAGAACTCCCGCCCGACGCGCGCCGAAGCCT
CCGACGTGGCCAACGCCGTGCTCGACGGTGCGGATGCCGTGATGCTCTCCGGCGAGACCTCCATCGGCAAGCACCCGATC
ACCACCGTGGAGACGATGGCTCGCATCGTGCGCGCCGCCGAGATTGACGGGGAAGTGCCGCCGCTGACGCACCGCCCGCG
CACCCGCCGCGGCGTGATTTCCTACGCGGCAAAGGACATCGGCGAGCGCCTGAATGCGAAGGCACTGGTGGCCTTCACCT
CCTCCGGCGATACCGCCAAGCGCGTGGCGCGCCTACGCTCCCACCTGCCGCTGCTGGTGTTCACGCCGTCGCAGGCCGTG
CGCTCGCAGCTGGCGCTGACGTGGGGCGTGGAGACGTTCCTCATCGAAGACGTGAAGTCCACCGACGAGATGATGGACAC
CGTCGACCACCACCTGCTGGCTATGCCGCAATACAAGTACGACGACATGATGGTCGTCGTCGCCGGCTCCCCGCCCGGGA
TCTCCGGCAACACCAACATGATCCAGGTGCACCTGCTGGGTCAGGAGCGGAAGAGGTAA

Upstream 100 bases:

>100_bases
ATTGTTTGTTTGGTGGACGGTGCAGCAACGCAAGACTAAGGGCGTCACAAAAGAAGAAACGAAGTAGCGGGGACAACGGT
GCGGGACTATCCTCGAGTCC

Downstream 100 bases:

>100_bases
CTCAGCGTCGCGGCCAGCGCCGCGTCCGTGGAGGTGCCCAGCGCCTCAATGCGCGGGGCGAAAGCACCGTGGTGGTTGCC
CGGCACCGGATCCCCGGAAG

Product: pyruvate kinase

Products: NA

Alternate protein names: PK [H]

Number of amino acids: Translated: 472; Mature: 472

Protein sequence:

>472_residues
MNRRTKIVCTLGPAVASKEQIRGLVDAGMNVARLNFSHGEHADHEQNYNWVRQATDESGKAVGILADLQGPKIRLGRFAE
GATVWATGETVRITVDDVQGTHDRVSTTYKGLARDARPGDRLLVDDGKIALVCKEVDGNDVVCEVIEGGPVSNNKGVSLP
GMNISVPALSEKDTEDLRFALQLGVDFIALSFVRSPSDVELVRDIMDEVGRRVPVIAKLEKPEAVDALEPVILAFDAIMV
ARGDLGVEVPLEDVPLVQKRAIQIARENAKPVIVATQMLDSMIENSRPTRAEASDVANAVLDGADAVMLSGETSIGKHPI
TTVETMARIVRAAEIDGEVPPLTHRPRTRRGVISYAAKDIGERLNAKALVAFTSSGDTAKRVARLRSHLPLLVFTPSQAV
RSQLALTWGVETFLIEDVKSTDEMMDTVDHHLLAMPQYKYDDMMVVVAGSPPGISGNTNMIQVHLLGQERKR

Sequences:

>Translated_472_residues
MNRRTKIVCTLGPAVASKEQIRGLVDAGMNVARLNFSHGEHADHEQNYNWVRQATDESGKAVGILADLQGPKIRLGRFAE
GATVWATGETVRITVDDVQGTHDRVSTTYKGLARDARPGDRLLVDDGKIALVCKEVDGNDVVCEVIEGGPVSNNKGVSLP
GMNISVPALSEKDTEDLRFALQLGVDFIALSFVRSPSDVELVRDIMDEVGRRVPVIAKLEKPEAVDALEPVILAFDAIMV
ARGDLGVEVPLEDVPLVQKRAIQIARENAKPVIVATQMLDSMIENSRPTRAEASDVANAVLDGADAVMLSGETSIGKHPI
TTVETMARIVRAAEIDGEVPPLTHRPRTRRGVISYAAKDIGERLNAKALVAFTSSGDTAKRVARLRSHLPLLVFTPSQAV
RSQLALTWGVETFLIEDVKSTDEMMDTVDHHLLAMPQYKYDDMMVVVAGSPPGISGNTNMIQVHLLGQERKR
>Mature_472_residues
MNRRTKIVCTLGPAVASKEQIRGLVDAGMNVARLNFSHGEHADHEQNYNWVRQATDESGKAVGILADLQGPKIRLGRFAE
GATVWATGETVRITVDDVQGTHDRVSTTYKGLARDARPGDRLLVDDGKIALVCKEVDGNDVVCEVIEGGPVSNNKGVSLP
GMNISVPALSEKDTEDLRFALQLGVDFIALSFVRSPSDVELVRDIMDEVGRRVPVIAKLEKPEAVDALEPVILAFDAIMV
ARGDLGVEVPLEDVPLVQKRAIQIARENAKPVIVATQMLDSMIENSRPTRAEASDVANAVLDGADAVMLSGETSIGKHPI
TTVETMARIVRAAEIDGEVPPLTHRPRTRRGVISYAAKDIGERLNAKALVAFTSSGDTAKRVARLRSHLPLLVFTPSQAV
RSQLALTWGVETFLIEDVKSTDEMMDTVDHHLLAMPQYKYDDMMVVVAGSPPGISGNTNMIQVHLLGQERKR

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI33286418, Length=489, Percent_Identity=37.6278118609407, Blast_Score=295, Evalue=4e-80,
Organism=Homo sapiens, GI33286422, Length=491, Percent_Identity=38.0855397148676, Blast_Score=295, Evalue=7e-80,
Organism=Homo sapiens, GI33286420, Length=491, Percent_Identity=38.0855397148676, Blast_Score=295, Evalue=7e-80,
Organism=Homo sapiens, GI32967597, Length=494, Percent_Identity=37.246963562753, Blast_Score=281, Evalue=1e-75,
Organism=Homo sapiens, GI10835121, Length=494, Percent_Identity=37.246963562753, Blast_Score=281, Evalue=1e-75,
Organism=Homo sapiens, GI310128732, Length=287, Percent_Identity=39.3728222996516, Blast_Score=200, Evalue=3e-51,
Organism=Homo sapiens, GI310128730, Length=287, Percent_Identity=39.3728222996516, Blast_Score=200, Evalue=3e-51,
Organism=Homo sapiens, GI310128736, Length=235, Percent_Identity=37.4468085106383, Blast_Score=161, Evalue=1e-39,
Organism=Homo sapiens, GI310128734, Length=235, Percent_Identity=37.4468085106383, Blast_Score=161, Evalue=1e-39,
Organism=Homo sapiens, GI310128738, Length=214, Percent_Identity=36.4485981308411, Blast_Score=140, Evalue=4e-33,
Organism=Escherichia coli, GI1787965, Length=472, Percent_Identity=41.1016949152542, Blast_Score=338, Evalue=5e-94,
Organism=Escherichia coli, GI1788160, Length=418, Percent_Identity=42.3444976076555, Blast_Score=298, Evalue=7e-82,
Organism=Caenorhabditis elegans, GI17544584, Length=421, Percent_Identity=38.2422802850356, Blast_Score=302, Evalue=2e-82,
Organism=Caenorhabditis elegans, GI17506829, Length=482, Percent_Identity=39.2116182572614, Blast_Score=293, Evalue=2e-79,
Organism=Caenorhabditis elegans, GI71984413, Length=482, Percent_Identity=39.2116182572614, Blast_Score=293, Evalue=2e-79,
Organism=Caenorhabditis elegans, GI71984406, Length=482, Percent_Identity=39.2116182572614, Blast_Score=292, Evalue=2e-79,
Organism=Caenorhabditis elegans, GI17506831, Length=482, Percent_Identity=39.2116182572614, Blast_Score=292, Evalue=2e-79,
Organism=Saccharomyces cerevisiae, GI6319279, Length=480, Percent_Identity=34.5833333333333, Blast_Score=261, Evalue=2e-70,
Organism=Saccharomyces cerevisiae, GI6324923, Length=487, Percent_Identity=33.0595482546201, Blast_Score=249, Evalue=9e-67,
Organism=Drosophila melanogaster, GI24648964, Length=486, Percent_Identity=38.0658436213992, Blast_Score=293, Evalue=2e-79,
Organism=Drosophila melanogaster, GI28571814, Length=486, Percent_Identity=38.0658436213992, Blast_Score=293, Evalue=2e-79,
Organism=Drosophila melanogaster, GI24648966, Length=413, Percent_Identity=36.0774818401937, Blast_Score=268, Evalue=7e-72,
Organism=Drosophila melanogaster, GI24581235, Length=483, Percent_Identity=32.2981366459627, Blast_Score=242, Evalue=4e-64,
Organism=Drosophila melanogaster, GI24646914, Length=283, Percent_Identity=33.5689045936396, Blast_Score=147, Evalue=1e-35,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 51218; Mature: 51218

Theoretical pI: Translated: 5.52; Mature: 5.52

Prosite motif: PS00110 PYRUVATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRRTKIVCTLGPAVASKEQIRGLVDAGMNVARLNFSHGEHADHEQNYNWVRQATDESGK
CCCCEEEEEEECCHHCCHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCHHHHHCCCCCCC
AVGILADLQGPKIRLGRFAEGATVWATGETVRITVDDVQGTHDRVSTTYKGLARDARPGD
EEEEEEECCCCCEEECEECCCCEEEECCCEEEEEEECCCCCHHHHHHHHHHHHCCCCCCC
RLLVDDGKIALVCKEVDGNDVVCEVIEGGPVSNNKGVSLPGMNISVPALSEKDTEDLRFA
EEEEECCCEEEEEEECCCCCEEEEEECCCCCCCCCCEECCCCEEECCCCCCCCHHHHHHH
LQLGVDFIALSFVRSPSDVELVRDIMDEVGRRVPVIAKLEKPEAVDALEPVILAFDAIMV
HHHCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHE
ARGDLGVEVPLEDVPLVQKRAIQIARENAKPVIVATQMLDSMIENSRPTRAEASDVANAV
ECCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEHHHHHHHHHHCCCCCCCHHHHHHHHH
LDGADAVMLSGETSIGKHPITTVETMARIVRAAEIDGEVPPLTHRPRTRRGVISYAAKDI
HCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHH
GERLNAKALVAFTSSGDTAKRVARLRSHLPLLVFTPSQAVRSQLALTWGVETFLIEDVKS
HHHCCCEEEEEEECCCHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHCCHHHHHHHHHHH
TDEMMDTVDHHLLAMPQYKYDDMMVVVAGSPPGISGNTNMIQVHLLGQERKR
HHHHHHHHHHHHEECCCCCCCCEEEEEECCCCCCCCCCCEEEEEEECCCCCC
>Mature Secondary Structure
MNRRTKIVCTLGPAVASKEQIRGLVDAGMNVARLNFSHGEHADHEQNYNWVRQATDESGK
CCCCEEEEEEECCHHCCHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCHHHHHCCCCCCC
AVGILADLQGPKIRLGRFAEGATVWATGETVRITVDDVQGTHDRVSTTYKGLARDARPGD
EEEEEEECCCCCEEECEECCCCEEEECCCEEEEEEECCCCCHHHHHHHHHHHHCCCCCCC
RLLVDDGKIALVCKEVDGNDVVCEVIEGGPVSNNKGVSLPGMNISVPALSEKDTEDLRFA
EEEEECCCEEEEEEECCCCCEEEEEECCCCCCCCCCEECCCCEEECCCCCCCCHHHHHHH
LQLGVDFIALSFVRSPSDVELVRDIMDEVGRRVPVIAKLEKPEAVDALEPVILAFDAIMV
HHHCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHE
ARGDLGVEVPLEDVPLVQKRAIQIARENAKPVIVATQMLDSMIENSRPTRAEASDVANAV
ECCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEHHHHHHHHHHCCCCCCCHHHHHHHHH
LDGADAVMLSGETSIGKHPITTVETMARIVRAAEIDGEVPPLTHRPRTRRGVISYAAKDI
HCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHH
GERLNAKALVAFTSSGDTAKRVARLRSHLPLLVFTPSQAVRSQLALTWGVETFLIEDVKS
HHHCCCEEEEEEECCCHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHCCHHHHHHHHHHH
TDEMMDTVDHHLLAMPQYKYDDMMVVVAGSPPGISGNTNMIQVHLLGQERKR
HHHHHHHHHHHHEECCCCCCCCEEEEEECCCCCCCCCCCEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8074528; 12948626 [H]