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The map label for this gene is pyk [H]

Identifier: 68535847

GI number: 68535847

Start: 923488

End: 925329

Strand: Direct

Name: pyk [H]

Synonym: jk0772

Alternate gene names: 68535847

Gene position: 923488-925329 (Clockwise)

Preceding gene: 68535846

Following gene: 68535849

Centisome position: 37.5

GC content: 66.02

Gene sequence:

>1842_bases
ATGCAGGTCAACAAGCAAGCCGTCCTAGAAGCGATCGAGGAGCTCCTCGACGAACTCGATAGCCAAACCCTGCACTGGTA
TCCGGAGATCCGCGCTACTGCGCCGACCCACTTCGTCGGCGCCCGCAACCTGGTGCACTACACCACGCTGCGCACCAGCG
ACCGCCGCGGCCTGCAGGGGAACCTGGAGAGCCTGGGTGCCACGCGCCTGTCTACCGCGGAGCCGGCGGTTAAGGCGCGC
TTACAAGCCGCGCACAACGTGGTCTCGGCTCTGGATGGTCGCTCGCCGCAGTTCGCTGAGGAGGACGTTGCGGACGCCAT
CGTGGGGGCCGATGAGCTGCTTGAACAGCACACGGAGGCTCTCTTCGGGACGGAGGCTTTGCCAGGGGACTCCTCGTACA
TCATGGTCACCCTGCCGATCGAGGCCGCCTACGATCAAGAACTGGTCGCCCGGCTGGTAGATTCCGGCATGGAGCTGGCG
CGCATCAACTGCGCCCACGACGGGCCGGAGGTGTGGAAGCGGATGATCGACAACGTCCGCGCGGCAGGCAAAGCTGCGGG
GCGTTACATTCCCATCAGCATGGATCTGGCCGGGCCGAAGATCCGCACCGGCCGCATCGCGCCGGGCCCGGCGGTGGTGC
GTGTGCGCGTGCAACGGGACGACGCGGGCACGGTGATCCAGCCCTGCCGCCTGTGGATGATCCCGGAGGACCAAGAGCTG
GCCGAGGCCCCCATGGCGGAGAACACGCTGGGCCGCCCCATCGTGAGCGTGCACGTGTCCCGGGAGTTCTTTGATGCCCT
GGAGGTTGGAGAGACTGTCCGTGTCGTGGATGCACGCGGCAAGAAGCGGAAGCTGGGCGTCATTAAGAAAGACAGCGAGG
GCGCCCTGGCGGAGGGGCAGCAGAACATCTACCTGCAGCTCGGCGCGGAGCTGAAAACGGAGAAGGCGAAGACGGCGGTG
GGAGCGGTGCCACAGGTGCTGCAGAGACTGCGGCCGCAGACCGGGGACAGGATTGTGCTGACCAGCGCCGACGTAGTGTG
CGACCCGCAGGCCGGTGGCATACCGAAGATCAGCTGTACGCTGCCGGAGGCGGTGCGGGCGCTCGAGGTGGGCCAGCAGG
TGCTGTTCGACGATGGGGCGATCGCGGCGAAGGTAGTGGAGATTCGGAAAGCCAAGGGCACAGAGGCCACAGCCGGGACA
GTCGAGGCGGAGTTGCTGGTCACCCGCGGCGGGGCGAAGCTGGCGGAGTACAAGGGCATTAACCTGCCTGACACGGAGTT
GCCGCTGCCGAGCCTGACCGAGGAGGACGAGGCCGCGCTGGCGTTCGTAGCCGAGTACGCGGATATGGCCGCGGTGAGCT
TCATCCGCTCTCGCGAGGACGTGGCCTATGTGCTGGAGAAGTTCCACGAGCTCGGCGCCGATGATCTAGGTCTGGTGCTG
AAGATTGAGACGATCCCCGCATTCGAGAACCTGACCACCATCCTGCTGGAGGGCATGCGGCACGCGAAGTTCGGCGTGAT
GCTGGCGCGCGGTGACCTGGCTGTGGAGATGGGCTTTGCGCGCATGGCGGAGGTGCCCGGCCAGGTGATGGCGATGGTGG
AGGCCGCACACGTGCCGTTGATCATCGCCACGCAGGTGCTGGAGTCGATGGCGAAGTCCGGGCTGCCGACCCGCGCGGAG
ATCACCGACGTGGCGTGGGCGCTGCGCGCCGAATGCATCATGCTGAACAAGGGGCCGCACATTCCGGAGGCTATCGCGAT
CCTGCGGGAGATCGGCGTGAAGATGGACCGCTCGCAGCGTAAGAACCGAATGTTGCTGCACCACGTGCGCAGCTGGCAAT
AA

Upstream 100 bases:

>100_bases
TCCCTTCGTCTTGCCGAGCGCAATCCCGGCAGACCTGTCACGATAGAAGGGACACAACGCTCGCGCAGCCGCGCACTTCC
CAAGGAGATGTCCCTTCACC

Downstream 100 bases:

>100_bases
CCGGCCAGCTGCGGCTAGCTACTGGGCGCTGTAGCGCGCCTCCCACGGACCGAGCCCCAGGCGGTCGTGGGGCGCGTCAG
CCAGGTTCACGGTTACCTTC

Product: pyruvate kinase

Products: NA

Alternate protein names: PK [H]

Number of amino acids: Translated: 613; Mature: 613

Protein sequence:

>613_residues
MQVNKQAVLEAIEELLDELDSQTLHWYPEIRATAPTHFVGARNLVHYTTLRTSDRRGLQGNLESLGATRLSTAEPAVKAR
LQAAHNVVSALDGRSPQFAEEDVADAIVGADELLEQHTEALFGTEALPGDSSYIMVTLPIEAAYDQELVARLVDSGMELA
RINCAHDGPEVWKRMIDNVRAAGKAAGRYIPISMDLAGPKIRTGRIAPGPAVVRVRVQRDDAGTVIQPCRLWMIPEDQEL
AEAPMAENTLGRPIVSVHVSREFFDALEVGETVRVVDARGKKRKLGVIKKDSEGALAEGQQNIYLQLGAELKTEKAKTAV
GAVPQVLQRLRPQTGDRIVLTSADVVCDPQAGGIPKISCTLPEAVRALEVGQQVLFDDGAIAAKVVEIRKAKGTEATAGT
VEAELLVTRGGAKLAEYKGINLPDTELPLPSLTEEDEAALAFVAEYADMAAVSFIRSREDVAYVLEKFHELGADDLGLVL
KIETIPAFENLTTILLEGMRHAKFGVMLARGDLAVEMGFARMAEVPGQVMAMVEAAHVPLIIATQVLESMAKSGLPTRAE
ITDVAWALRAECIMLNKGPHIPEAIAILREIGVKMDRSQRKNRMLLHHVRSWQ

Sequences:

>Translated_613_residues
MQVNKQAVLEAIEELLDELDSQTLHWYPEIRATAPTHFVGARNLVHYTTLRTSDRRGLQGNLESLGATRLSTAEPAVKAR
LQAAHNVVSALDGRSPQFAEEDVADAIVGADELLEQHTEALFGTEALPGDSSYIMVTLPIEAAYDQELVARLVDSGMELA
RINCAHDGPEVWKRMIDNVRAAGKAAGRYIPISMDLAGPKIRTGRIAPGPAVVRVRVQRDDAGTVIQPCRLWMIPEDQEL
AEAPMAENTLGRPIVSVHVSREFFDALEVGETVRVVDARGKKRKLGVIKKDSEGALAEGQQNIYLQLGAELKTEKAKTAV
GAVPQVLQRLRPQTGDRIVLTSADVVCDPQAGGIPKISCTLPEAVRALEVGQQVLFDDGAIAAKVVEIRKAKGTEATAGT
VEAELLVTRGGAKLAEYKGINLPDTELPLPSLTEEDEAALAFVAEYADMAAVSFIRSREDVAYVLEKFHELGADDLGLVL
KIETIPAFENLTTILLEGMRHAKFGVMLARGDLAVEMGFARMAEVPGQVMAMVEAAHVPLIIATQVLESMAKSGLPTRAE
ITDVAWALRAECIMLNKGPHIPEAIAILREIGVKMDRSQRKNRMLLHHVRSWQ
>Mature_613_residues
MQVNKQAVLEAIEELLDELDSQTLHWYPEIRATAPTHFVGARNLVHYTTLRTSDRRGLQGNLESLGATRLSTAEPAVKAR
LQAAHNVVSALDGRSPQFAEEDVADAIVGADELLEQHTEALFGTEALPGDSSYIMVTLPIEAAYDQELVARLVDSGMELA
RINCAHDGPEVWKRMIDNVRAAGKAAGRYIPISMDLAGPKIRTGRIAPGPAVVRVRVQRDDAGTVIQPCRLWMIPEDQEL
AEAPMAENTLGRPIVSVHVSREFFDALEVGETVRVVDARGKKRKLGVIKKDSEGALAEGQQNIYLQLGAELKTEKAKTAV
GAVPQVLQRLRPQTGDRIVLTSADVVCDPQAGGIPKISCTLPEAVRALEVGQQVLFDDGAIAAKVVEIRKAKGTEATAGT
VEAELLVTRGGAKLAEYKGINLPDTELPLPSLTEEDEAALAFVAEYADMAAVSFIRSREDVAYVLEKFHELGADDLGLVL
KIETIPAFENLTTILLEGMRHAKFGVMLARGDLAVEMGFARMAEVPGQVMAMVEAAHVPLIIATQVLESMAKSGLPTRAE
ITDVAWALRAECIMLNKGPHIPEAIAILREIGVKMDRSQRKNRMLLHHVRSWQ

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI33286418, Length=230, Percent_Identity=36.0869565217391, Blast_Score=103, Evalue=4e-22,
Organism=Homo sapiens, GI33286422, Length=230, Percent_Identity=36.0869565217391, Blast_Score=103, Evalue=5e-22,
Organism=Homo sapiens, GI33286420, Length=230, Percent_Identity=36.0869565217391, Blast_Score=103, Evalue=5e-22,
Organism=Homo sapiens, GI10835121, Length=240, Percent_Identity=34.5833333333333, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI32967597, Length=240, Percent_Identity=34.5833333333333, Blast_Score=97, Evalue=4e-20,
Organism=Homo sapiens, GI310128732, Length=183, Percent_Identity=32.2404371584699, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI310128730, Length=183, Percent_Identity=32.2404371584699, Blast_Score=71, Evalue=2e-12,
Organism=Escherichia coli, GI1787965, Length=239, Percent_Identity=35.1464435146443, Blast_Score=110, Evalue=4e-25,
Organism=Escherichia coli, GI1788160, Length=201, Percent_Identity=33.3333333333333, Blast_Score=77, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17544584, Length=239, Percent_Identity=34.3096234309623, Blast_Score=95, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17506831, Length=233, Percent_Identity=34.3347639484979, Blast_Score=92, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI71984406, Length=233, Percent_Identity=34.3347639484979, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI71984413, Length=233, Percent_Identity=34.3347639484979, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17506829, Length=233, Percent_Identity=34.3347639484979, Blast_Score=91, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6319279, Length=235, Percent_Identity=33.6170212765957, Blast_Score=111, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6324923, Length=225, Percent_Identity=32.8888888888889, Blast_Score=100, Evalue=6e-22,
Organism=Drosophila melanogaster, GI24648966, Length=272, Percent_Identity=31.25, Blast_Score=104, Evalue=2e-22,
Organism=Drosophila melanogaster, GI28571814, Length=247, Percent_Identity=32.7935222672065, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24648964, Length=247, Percent_Identity=32.7935222672065, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24581235, Length=218, Percent_Identity=25.2293577981651, Blast_Score=71, Evalue=2e-12,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 66615; Mature: 66615

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQVNKQAVLEAIEELLDELDSQTLHWYPEIRATAPTHFVGARNLVHYTTLRTSDRRGLQG
CCCCHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCHHHCCCCEEEEEEEECCCCCCCCC
NLESLGATRLSTAEPAVKARLQAAHNVVSALDGRSPQFAEEDVADAIVGADELLEQHTEA
CHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCHHHHHHHHHHH
LFGTEALPGDSSYIMVTLPIEAAYDQELVARLVDSGMELARINCAHDGPEVWKRMIDNVR
HHCCCCCCCCCCEEEEEEECCHHCCHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHH
AAGKAAGRYIPISMDLAGPKIRTGRIAPGPAVVRVRVQRDDAGTVIQPCRLWMIPEDQEL
HHHHCCCCEEEEEEECCCCCEECCCCCCCCEEEEEEEECCCCCCEECCCEEEECCCCHHH
AEAPMAENTLGRPIVSVHVSREFFDALEVGETVRVVDARGKKRKLGVIKKDSEGALAEGQ
HHCCCCCCCCCCCEEEEEECHHHHHHHHCCCEEEEEECCCCCCCCCEEEECCCCCCCCCC
QNIYLQLGAELKTEKAKTAVGAVPQVLQRLRPQTGDRIVLTSADVVCDPQAGGIPKISCT
CEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCEEECCCCCCCCEEEEC
LPEAVRALEVGQQVLFDDGAIAAKVVEIRKAKGTEATAGTVEAELLVTRGGAKLAEYKGI
CHHHHHHHHHCHHHEECCCCHHHHHHHHHHCCCCCCCCCCEEEEEEEECCCCHHHHCCCC
NLPDTELPLPSLTEEDEAALAFVAEYADMAAVSFIRSREDVAYVLEKFHELGADDLGLVL
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEE
KIETIPAFENLTTILLEGMRHAKFGVMLARGDLAVEMGFARMAEVPGQVMAMVEAAHVPL
EEECCCCHHHHHHHHHHHHHHHHHEEEEECCCEEEHHHHHHHHHCCHHHHHHHHHHCCCH
IIATQVLESMAKSGLPTRAEITDVAWALRAECIMLNKGPHIPEAIAILREIGVKMDRSQR
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCEEEECCCCCCHHHHHHHHHHCCCCCHHHH
KNRMLLHHVRSWQ
HHHHHHHHHHCCC
>Mature Secondary Structure
MQVNKQAVLEAIEELLDELDSQTLHWYPEIRATAPTHFVGARNLVHYTTLRTSDRRGLQG
CCCCHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCHHHCCCCEEEEEEEECCCCCCCCC
NLESLGATRLSTAEPAVKARLQAAHNVVSALDGRSPQFAEEDVADAIVGADELLEQHTEA
CHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCHHHHHHHHHHH
LFGTEALPGDSSYIMVTLPIEAAYDQELVARLVDSGMELARINCAHDGPEVWKRMIDNVR
HHCCCCCCCCCCEEEEEEECCHHCCHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHH
AAGKAAGRYIPISMDLAGPKIRTGRIAPGPAVVRVRVQRDDAGTVIQPCRLWMIPEDQEL
HHHHCCCCEEEEEEECCCCCEECCCCCCCCEEEEEEEECCCCCCEECCCEEEECCCCHHH
AEAPMAENTLGRPIVSVHVSREFFDALEVGETVRVVDARGKKRKLGVIKKDSEGALAEGQ
HHCCCCCCCCCCCEEEEEECHHHHHHHHCCCEEEEEECCCCCCCCCEEEECCCCCCCCCC
QNIYLQLGAELKTEKAKTAVGAVPQVLQRLRPQTGDRIVLTSADVVCDPQAGGIPKISCT
CEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCEEECCCCCCCCEEEEC
LPEAVRALEVGQQVLFDDGAIAAKVVEIRKAKGTEATAGTVEAELLVTRGGAKLAEYKGI
CHHHHHHHHHCHHHEECCCCHHHHHHHHHHCCCCCCCCCCEEEEEEEECCCCHHHHCCCC
NLPDTELPLPSLTEEDEAALAFVAEYADMAAVSFIRSREDVAYVLEKFHELGADDLGLVL
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEE
KIETIPAFENLTTILLEGMRHAKFGVMLARGDLAVEMGFARMAEVPGQVMAMVEAAHVPL
EEECCCCHHHHHHHHHHHHHHHHHEEEEECCCEEEHHHHHHHHHCCHHHHHHHHHHCCCH
IIATQVLESMAKSGLPTRAEITDVAWALRAECIMLNKGPHIPEAIAILREIGVKMDRSQR
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCEEEECCCCCCHHHHHHHHHHCCCCCHHHH
KNRMLLHHVRSWQ
HHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA