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The map label for this gene is metF [H]

Identifier: 68535812

GI number: 68535812

Start: 881083

End: 882051

Strand: Direct

Name: metF [H]

Synonym: jk0737

Alternate gene names: 68535812

Gene position: 881083-882051 (Clockwise)

Preceding gene: 68535807

Following gene: 68535814

Centisome position: 35.78

GC content: 63.57

Gene sequence:

>969_bases
ATGGCTAGTATCCGCAGGCAGGTTGCCGTATCCCAGTCCCTTTCGCTCAACACTCCGGGGCGCGTTCCGTTCTCTGTAGA
GTTCATGCCGCCGCGCGACGACGCGGCCGAAGAGCGCCTGTGGAACGCTGCCGAGGCTTTCCACGACCTCGGCGTAAGCT
TCGCCTCCGTGACCTATGGTGCCGGCGGTAGCACCCGCGAACGTACCCTGCGCGTTGCAGAGCGCCTGGGCACCAAGCCG
CTAACAACCCTGGTGCACATGACGTTGGTGCAGCACACCCAGGAAGAGCTGCAGGATATGCTGCGCACCTATGCCTCGCT
GGGGCTCACCAACCTACTGGCACTACGCGGCGACCCGCCGGGGGATCCGAACGCCGAATGGGTACCCACCCCGGGTGGTT
TGAGCTACGCCAGCGAGCTGATCGAATTGATCCGCGAAATGCCCGAGTGTGCCGACTTTGATATCGGCATCGCCAGCTTC
CCCGAAGGCCACTACCGCACGGGTTCTTTGGAGCAAGACACGGAATACACCCTGGCCAAGCTCCGCGCCGGGGCGCAGTA
CTCGATCACGCAAATGTTCTTTGACGTGGACCACTACTTGCGGCTGCGCGATCGCCTGGCGAAAGCGGATCCGGAGCATG
GCCAGAAGCCGATCATCCCGGGGCTGATGCCGATTACCTCGCTGCGGAGCGTGCGCCGCCAGATGGAGTTGGCGGGCGCC
GCGCTGCCCCCGAAGTTGGAAAAACGTCTGCTGGATGCCGCAGCTGGAGACGAAGTAGCCAACCGCGATGCCATCCGCGA
GGTTGGCATTGAGGTGACGACCGAGATGGCCGAGCGTCTCATCGCTGAAGGTGTTCCGGACCTGCACTTTATGACTATGA
ACAATGTGCGCGCCACCCAGGAGGTCCTGCATAACCTGGGCATGGCTCCGGCCTGGGGGCCGGGGCTGGGCCACGACATG
GTGCGCTAG

Upstream 100 bases:

>100_bases
AGACTCACGGACGGGCTACGGGGGTGATTGCGCGCACGGAAATTACCGAAAATTATCCAATATCTTGAAATGGACAGCTC
TGTCTATATGATCTTTGGGT

Downstream 100 bases:

>100_bases
GGTGCGAAATGGGAGTGTTAGGGGCGCGTGCGCTGGAGGATTCCGAAAGGCCTGGGATCTGCCGGGAACTTAAAATTCCG
CAGAACATCGGTGAACCCAG

Product: 5,10-methylenetetrahydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 322; Mature: 321

Protein sequence:

>322_residues
MASIRRQVAVSQSLSLNTPGRVPFSVEFMPPRDDAAEERLWNAAEAFHDLGVSFASVTYGAGGSTRERTLRVAERLGTKP
LTTLVHMTLVQHTQEELQDMLRTYASLGLTNLLALRGDPPGDPNAEWVPTPGGLSYASELIELIREMPECADFDIGIASF
PEGHYRTGSLEQDTEYTLAKLRAGAQYSITQMFFDVDHYLRLRDRLAKADPEHGQKPIIPGLMPITSLRSVRRQMELAGA
ALPPKLEKRLLDAAAGDEVANRDAIREVGIEVTTEMAERLIAEGVPDLHFMTMNNVRATQEVLHNLGMAPAWGPGLGHDM
VR

Sequences:

>Translated_322_residues
MASIRRQVAVSQSLSLNTPGRVPFSVEFMPPRDDAAEERLWNAAEAFHDLGVSFASVTYGAGGSTRERTLRVAERLGTKP
LTTLVHMTLVQHTQEELQDMLRTYASLGLTNLLALRGDPPGDPNAEWVPTPGGLSYASELIELIREMPECADFDIGIASF
PEGHYRTGSLEQDTEYTLAKLRAGAQYSITQMFFDVDHYLRLRDRLAKADPEHGQKPIIPGLMPITSLRSVRRQMELAGA
ALPPKLEKRLLDAAAGDEVANRDAIREVGIEVTTEMAERLIAEGVPDLHFMTMNNVRATQEVLHNLGMAPAWGPGLGHDM
VR
>Mature_321_residues
ASIRRQVAVSQSLSLNTPGRVPFSVEFMPPRDDAAEERLWNAAEAFHDLGVSFASVTYGAGGSTRERTLRVAERLGTKPL
TTLVHMTLVQHTQEELQDMLRTYASLGLTNLLALRGDPPGDPNAEWVPTPGGLSYASELIELIREMPECADFDIGIASFP
EGHYRTGSLEQDTEYTLAKLRAGAQYSITQMFFDVDHYLRLRDRLAKADPEHGQKPIIPGLMPITSLRSVRRQMELAGAA
LPPKLEKRLLDAAAGDEVANRDAIREVGIEVTTEMAERLIAEGVPDLHFMTMNNVRATQEVLHNLGMAPAWGPGLGHDMV
R

Specific function: Methionine biosynthesis. [C]

COG id: COG0685

COG function: function code E; 5,10-methylenetetrahydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methylenetetrahydrofolate reductase family [H]

Homologues:

Organism=Homo sapiens, GI87240000, Length=293, Percent_Identity=35.8361774744027, Blast_Score=157, Evalue=1e-38,
Organism=Escherichia coli, GI1790377, Length=308, Percent_Identity=35.3896103896104, Blast_Score=171, Evalue=6e-44,
Organism=Caenorhabditis elegans, GI25147436, Length=294, Percent_Identity=31.6326530612245, Blast_Score=121, Evalue=6e-28,
Organism=Caenorhabditis elegans, GI25147441, Length=294, Percent_Identity=31.6326530612245, Blast_Score=121, Evalue=6e-28,
Organism=Saccharomyces cerevisiae, GI6321313, Length=301, Percent_Identity=31.2292358803987, Blast_Score=137, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6325234, Length=300, Percent_Identity=29.3333333333333, Blast_Score=128, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24662658, Length=248, Percent_Identity=26.2096774193548, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003171
- InterPro:   IPR004620 [H]

Pfam domain/function: PF02219 MTHFR [H]

EC number: =1.5.1.20 [H]

Molecular weight: Translated: 35495; Mature: 35364

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASIRRQVAVSQSLSLNTPGRVPFSVEFMPPRDDAAEERLWNAAEAFHDLGVSFASVTYG
CCHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCHHHHHEEC
AGGSTRERTLRVAERLGTKPLTTLVHMTLVQHTQEELQDMLRTYASLGLTNLLALRGDPP
CCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
GDPNAEWVPTPGGLSYASELIELIREMPECADFDIGIASFPEGHYRTGSLEQDTEYTLAK
CCCCCCEECCCCCHHHHHHHHHHHHHCHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
LRAGAQYSITQMFFDVDHYLRLRDRLAKADPEHGQKPIIPGLMPITSLRSVRRQMELAGA
HHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCC
ALPPKLEKRLLDAAAGDEVANRDAIREVGIEVTTEMAERLIAEGVPDLHFMTMNNVRATQ
CCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHH
EVLHNLGMAPAWGPGLGHDMVR
HHHHHCCCCCCCCCCCCHHCCC
>Mature Secondary Structure 
ASIRRQVAVSQSLSLNTPGRVPFSVEFMPPRDDAAEERLWNAAEAFHDLGVSFASVTYG
CHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCHHHHHEEC
AGGSTRERTLRVAERLGTKPLTTLVHMTLVQHTQEELQDMLRTYASLGLTNLLALRGDPP
CCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
GDPNAEWVPTPGGLSYASELIELIREMPECADFDIGIASFPEGHYRTGSLEQDTEYTLAK
CCCCCCEECCCCCHHHHHHHHHHHHHCHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
LRAGAQYSITQMFFDVDHYLRLRDRLAKADPEHGQKPIIPGLMPITSLRSVRRQMELAGA
HHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCC
ALPPKLEKRLLDAAAGDEVANRDAIREVGIEVTTEMAERLIAEGVPDLHFMTMNNVRATQ
CCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHH
EVLHNLGMAPAWGPGLGHDMVR
HHHHHCCCCCCCCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9515933 [H]