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The map label for this gene is poxB [H]

Identifier: 68535790

GI number: 68535790

Start: 854134

End: 855894

Strand: Direct

Name: poxB [H]

Synonym: jk0715

Alternate gene names: 68535790

Gene position: 854134-855894 (Clockwise)

Preceding gene: 68535788

Following gene: 68535791

Centisome position: 34.69

GC content: 60.42

Gene sequence:

>1761_bases
ATGGCTAAAAACTTCGCATCTCAGCTGGTAAAGACTCTAGAAAAGCAAGGCGTTAAGCGGATCTTCGGACTGGTGGGTGA
TAGCCTCAACCCGATTGTCGACGAGGTTCGCCAGTCTTCCATCGAATGGATCCACGTGCGCAACGAGGAAGCCGCGGCTT
TCGCCGCAGGCGCGGAATCACTCGTCACCGGCGATCTAGCCGTGTGTGCCGCTTCTTGTGGACCGGGTAACACCCACTTG
ATCCAGGGCCTTTATGATTCCCACCGCAACGGTGCAAAGCTACTGGCCATCGCTTCTCACATCCCGAGTGTGCAGATCGG
TAGCCAGTTCTTCCAGGAGACCCACCCGGAGCAGATCTTCCAGGAGTGCTCCGGCTACTGCGAGATGGTGAACTCCGCAG
CGCAGGGACCAAAGGTTCTGCACCACGCCATCCAGTCCACGTTGGCCGGCAACGGTGTCAGCGTGCTGGTCATCCCCGGC
GATGTATCTTCCCAGCCGATTGAGGAAGAAGGCTTCATCGACTCGGTGTACTCCAAGGGGCACCCGGTGCTTTACCCGGA
TGCCGAGGAAGCAGCCCGCCTAGCCAAGGCGATTAACGATGCTAAGACCGTCACTTTGTTCTGCGGTGAGGGCGTCAAGA
ATTCTCGAGAGAAAGTGCTCGAGCTTGCAGAGAAGATTAAGTCTCCGATCGGTCACGCCTTCGGCGGAAAGATGCACATC
CAATACGACAACCCGTTCGACGTGGGCATGTCCGGCCTGCTGGGCTACGGTGCGGCGCGCGAGGCGATGTACGACGCTGA
CCTGTTGATTCTGCTGGGTACCGATTTCCCGTACACCGACTTCCTGCCCACCGGCAACGTAGCGCAGGTGGACATCGACG
GCTCCCACATCGGCCGCCGCACCACTATCGACTACCCGGTAGTCGGCGACGTCGGGGCTGTGATCGATACGATCCTGCCG
CACATCGAGGAAAAGACCGACCGGAGCTTCCTGGATAAGCAGCTGCGCAACCATGCGCGAGCACTGAATCACGTTGTGGA
TGCCTACACCAAGAAGGCGGGCAAGTCGAAGCCTATCCACCCGGAGTATGTGGCGGACACGATCGACCGCCTGGCCGCCG
AGGATGCAATCTTCACCATTGATACCGGCATGTGCAACGTCTGGGGTGCCCGCTACATCACGCCTAACGGCAAGCGCCAG
GAAATCGGTTCCTTCCGCCACGGCACCATGGCCAACGCACTGCCGCAGGCGCTGGGTGCGCAGGCCGCGGACCGCGACCG
TCAGGTGATCTCCTTCTCCGGCGATGGTGGCCTGGGAATGCTCATGGGTGAGCTGCTGACCGCCAAGTTGCACGACCTGC
CGGTGAACATCGTGGTCTTCAACAACTCCTCGCTGGGCATGGTCAAGCTGGAGATGATGGTTGCCGGCCTGCCGGAACAC
GAAACCGACCACGAGCACGTGAACTTCGCCAAGATCGCGGAAGCCATCGGAATTAAGGCGATCCGCATCGAGGATCCGGC
GGACGTGGAGAGCGGTATTAAGGAAGCTCTGGCTTACGACGGCCCGGTGCTGGTGGATGTGGTTACCGACCCGAACGCGC
TGAGCCTGCCGCCGAACATCACCTGGGAAATGATGATGGGCTTCTCCCGCTCCGCAACCCGCACCGTATTCGAGGGTGGC
GTGGGGCGCATGGTCGACCTGGCCCGCGCCAACCTGCGCAACATCGGCGGTGCCGGAGTGATCGAGATGAACAAGTTCTA
G

Upstream 100 bases:

>100_bases
GTTTTCTAGTACCTGAGTGATGTGTATTACATGAATTTACTTCTGTGAGACTGTCTAGCGATCCATGCGCTGACGTAAAG
CCCCGCGTAGCCTTGGAGGC

Downstream 100 bases:

>100_bases
AAGGGGTAGCGGAGTTGCTAGGGCGCTCTAGAGAGCCTTAGAGGTGCCCTATAAGCCATCAGAAAGGCGGGAGTCACGCA
ATGCGCGGGCTCCCGCCTAA

Product: pyruvate dehydrogenase

Products: NA

Alternate protein names: Pyruvate oxidase; POX [H]

Number of amino acids: Translated: 586; Mature: 585

Protein sequence:

>586_residues
MAKNFASQLVKTLEKQGVKRIFGLVGDSLNPIVDEVRQSSIEWIHVRNEEAAAFAAGAESLVTGDLAVCAASCGPGNTHL
IQGLYDSHRNGAKLLAIASHIPSVQIGSQFFQETHPEQIFQECSGYCEMVNSAAQGPKVLHHAIQSTLAGNGVSVLVIPG
DVSSQPIEEEGFIDSVYSKGHPVLYPDAEEAARLAKAINDAKTVTLFCGEGVKNSREKVLELAEKIKSPIGHAFGGKMHI
QYDNPFDVGMSGLLGYGAAREAMYDADLLILLGTDFPYTDFLPTGNVAQVDIDGSHIGRRTTIDYPVVGDVGAVIDTILP
HIEEKTDRSFLDKQLRNHARALNHVVDAYTKKAGKSKPIHPEYVADTIDRLAAEDAIFTIDTGMCNVWGARYITPNGKRQ
EIGSFRHGTMANALPQALGAQAADRDRQVISFSGDGGLGMLMGELLTAKLHDLPVNIVVFNNSSLGMVKLEMMVAGLPEH
ETDHEHVNFAKIAEAIGIKAIRIEDPADVESGIKEALAYDGPVLVDVVTDPNALSLPPNITWEMMMGFSRSATRTVFEGG
VGRMVDLARANLRNIGGAGVIEMNKF

Sequences:

>Translated_586_residues
MAKNFASQLVKTLEKQGVKRIFGLVGDSLNPIVDEVRQSSIEWIHVRNEEAAAFAAGAESLVTGDLAVCAASCGPGNTHL
IQGLYDSHRNGAKLLAIASHIPSVQIGSQFFQETHPEQIFQECSGYCEMVNSAAQGPKVLHHAIQSTLAGNGVSVLVIPG
DVSSQPIEEEGFIDSVYSKGHPVLYPDAEEAARLAKAINDAKTVTLFCGEGVKNSREKVLELAEKIKSPIGHAFGGKMHI
QYDNPFDVGMSGLLGYGAAREAMYDADLLILLGTDFPYTDFLPTGNVAQVDIDGSHIGRRTTIDYPVVGDVGAVIDTILP
HIEEKTDRSFLDKQLRNHARALNHVVDAYTKKAGKSKPIHPEYVADTIDRLAAEDAIFTIDTGMCNVWGARYITPNGKRQ
EIGSFRHGTMANALPQALGAQAADRDRQVISFSGDGGLGMLMGELLTAKLHDLPVNIVVFNNSSLGMVKLEMMVAGLPEH
ETDHEHVNFAKIAEAIGIKAIRIEDPADVESGIKEALAYDGPVLVDVVTDPNALSLPPNITWEMMMGFSRSATRTVFEGG
VGRMVDLARANLRNIGGAGVIEMNKF
>Mature_585_residues
AKNFASQLVKTLEKQGVKRIFGLVGDSLNPIVDEVRQSSIEWIHVRNEEAAAFAAGAESLVTGDLAVCAASCGPGNTHLI
QGLYDSHRNGAKLLAIASHIPSVQIGSQFFQETHPEQIFQECSGYCEMVNSAAQGPKVLHHAIQSTLAGNGVSVLVIPGD
VSSQPIEEEGFIDSVYSKGHPVLYPDAEEAARLAKAINDAKTVTLFCGEGVKNSREKVLELAEKIKSPIGHAFGGKMHIQ
YDNPFDVGMSGLLGYGAAREAMYDADLLILLGTDFPYTDFLPTGNVAQVDIDGSHIGRRTTIDYPVVGDVGAVIDTILPH
IEEKTDRSFLDKQLRNHARALNHVVDAYTKKAGKSKPIHPEYVADTIDRLAAEDAIFTIDTGMCNVWGARYITPNGKRQE
IGSFRHGTMANALPQALGAQAADRDRQVISFSGDGGLGMLMGELLTAKLHDLPVNIVVFNNSSLGMVKLEMMVAGLPEHE
TDHEHVNFAKIAEAIGIKAIRIEDPADVESGIKEALAYDGPVLVDVVTDPNALSLPPNITWEMMMGFSRSATRTVFEGGV
GRMVDLARANLRNIGGAGVIEMNKF

Specific function: Unknown

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=567, Percent_Identity=22.9276895943563, Blast_Score=121, Evalue=2e-27,
Organism=Escherichia coli, GI1787096, Length=573, Percent_Identity=48.3420593368237, Blast_Score=536, Evalue=1e-153,
Organism=Escherichia coli, GI1790104, Length=542, Percent_Identity=29.8892988929889, Blast_Score=201, Evalue=9e-53,
Organism=Escherichia coli, GI87081685, Length=554, Percent_Identity=27.7978339350181, Blast_Score=181, Evalue=1e-46,
Organism=Escherichia coli, GI1786717, Length=551, Percent_Identity=25.5898366606171, Blast_Score=152, Evalue=8e-38,
Organism=Escherichia coli, GI1788716, Length=549, Percent_Identity=20.4007285974499, Blast_Score=77, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17531299, Length=480, Percent_Identity=24.7916666666667, Blast_Score=95, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17531301, Length=480, Percent_Identity=24.7916666666667, Blast_Score=95, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6323755, Length=563, Percent_Identity=25.7548845470693, Blast_Score=169, Evalue=8e-43,
Organism=Saccharomyces cerevisiae, GI6320816, Length=492, Percent_Identity=23.780487804878, Blast_Score=78, Evalue=4e-15,
Organism=Drosophila melanogaster, GI19922626, Length=569, Percent_Identity=20.9138840070299, Blast_Score=101, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =1.2.5.1 [H]

Molecular weight: Translated: 63088; Mature: 62957

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKNFASQLVKTLEKQGVKRIFGLVGDSLNPIVDEVRQSSIEWIHVRNEEAAAFAAGAES
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEEEECCCHHHHHHCCHH
LVTGDLAVCAASCGPGNTHLIQGLYDSHRNGAKLLAIASHIPSVQIGSQFFQETHPEQIF
HHHCCHHHHEECCCCCCHHHHHHHHHCCCCCCEEEEEHHHCCCHHHHHHHHHHCCHHHHH
QECSGYCEMVNSAAQGPKVLHHAIQSTLAGNGVSVLVIPGDVSSQPIEEEGFIDSVYSKG
HHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCHHHHHCCC
HPVLYPDAEEAARLAKAINDAKTVTLFCGEGVKNSREKVLELAEKIKSPIGHAFGGKMHI
CCEECCCHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCHHCCCEEEE
QYDNPFDVGMSGLLGYGAAREAMYDADLLILLGTDFPYTDFLPTGNVAQVDIDGSHIGRR
EECCCHHHCHHHHHHCCHHHHHHCCCCEEEEEECCCCCCCCCCCCCEEEEEECCCCCCCC
TTIDYPVVGDVGAVIDTILPHIEEKTDRSFLDKQLRNHARALNHVVDAYTKKAGKSKPIH
EECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
PEYVADTIDRLAAEDAIFTIDTGMCNVWGARYITPNGKRQEIGSFRHGTMANALPQALGA
HHHHHHHHHHHHHCCCEEEEECCCCCCCCCEEECCCCCHHHHCCCCCCHHHHHHHHHHCC
QAADRDRQVISFSGDGGLGMLMGELLTAKLHDLPVNIVVFNNSSLGMVKLEMMVAGLPEH
CCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCEEHHHHHHCCCCC
ETDHEHVNFAKIAEAIGIKAIRIEDPADVESGIKEALAYDGPVLVDVVTDPNALSLPPNI
CCCCCCCCHHHHHHHHCEEEEEECCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCC
TWEMMMGFSRSATRTVFEGGVGRMVDLARANLRNIGGAGVIEMNKF
HHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHCCCCCEEEECCC
>Mature Secondary Structure 
AKNFASQLVKTLEKQGVKRIFGLVGDSLNPIVDEVRQSSIEWIHVRNEEAAAFAAGAES
CCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEEEECCCHHHHHHCCHH
LVTGDLAVCAASCGPGNTHLIQGLYDSHRNGAKLLAIASHIPSVQIGSQFFQETHPEQIF
HHHCCHHHHEECCCCCCHHHHHHHHHCCCCCCEEEEEHHHCCCHHHHHHHHHHCCHHHHH
QECSGYCEMVNSAAQGPKVLHHAIQSTLAGNGVSVLVIPGDVSSQPIEEEGFIDSVYSKG
HHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCHHHHHCCC
HPVLYPDAEEAARLAKAINDAKTVTLFCGEGVKNSREKVLELAEKIKSPIGHAFGGKMHI
CCEECCCHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCHHCCCEEEE
QYDNPFDVGMSGLLGYGAAREAMYDADLLILLGTDFPYTDFLPTGNVAQVDIDGSHIGRR
EECCCHHHCHHHHHHCCHHHHHHCCCCEEEEEECCCCCCCCCCCCCEEEEEECCCCCCCC
TTIDYPVVGDVGAVIDTILPHIEEKTDRSFLDKQLRNHARALNHVVDAYTKKAGKSKPIH
EECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
PEYVADTIDRLAAEDAIFTIDTGMCNVWGARYITPNGKRQEIGSFRHGTMANALPQALGA
HHHHHHHHHHHHHCCCEEEEECCCCCCCCCEEECCCCCHHHHCCCCCCHHHHHHHHHHCC
QAADRDRQVISFSGDGGLGMLMGELLTAKLHDLPVNIVVFNNSSLGMVKLEMMVAGLPEH
CCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCEEHHHHHHCCCCC
ETDHEHVNFAKIAEAIGIKAIRIEDPADVESGIKEALAYDGPVLVDVVTDPNALSLPPNI
CCCCCCCCHHHHHHHHCEEEEEECCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCC
TWEMMMGFSRSATRTVFEGGVGRMVDLARANLRNIGGAGVIEMNKF
HHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHCCCCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3016647; 8905232; 9278503; 2663858; 3902830; 8022274 [H]