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The map label for this gene is dnaG [H]

Identifier: 68535702

GI number: 68535702

Start: 758219

End: 760204

Strand: Direct

Name: dnaG [H]

Synonym: jk0630

Alternate gene names: 68535702

Gene position: 758219-760204 (Clockwise)

Preceding gene: 68535699

Following gene: 68535703

Centisome position: 30.79

GC content: 63.9

Gene sequence:

>1986_bases
GTGGCTATGGCTAAGGGCAGGATTCCGGAGCGGGACATCGAAGCGATTCGAGAGCAGACCCCCATCGAAGAAGTAGTGGG
GGAGTATGTGCAGCTCAAACCGGGTGGCGTCGATTCCATGAAGGGCCTGAGCCCCTTTAAGGACGAAAAGACTCCCTCGT
TCCACGTCCGCCCGAACCACGGCTACTTCCACTGCTTCTCCACCGACCAGGGCGGCGACGTTTTCAAGTTCCTCATGCTG
ATGGAACACATGTCCTTCCCCGAGGCCGTGGAGGCCTGCGCCGAGCGCATCGGCTACCGCATTAGCTACGAGGGCGGTCG
ACCGGGCAATCGGGAGGAGCCCGGCACGCGGCGCAGATTAGTCGCCGCGAACAAGGCGGCCTTTGAGTTTTATCGCAAGG
AATTTCTTGGTGACGCCGAAGGCGCGGACGTTGCCCGCAACTTCCTGATCGAGCGCGGTTTCACCATGGAACACGCCGAG
CAGTTCGGCTGTGGGTATGCGCCCGCCGGATGGGATCGGCTGACTAAGCACCTGCAGCAGAAGGGCTTTACGTTCAAGGA
AATCGAGGCGGCGGGCCTGGGCCGCATGGGTAAGCGCGGTCCGATCGACCGGTTCCACCGCCGCCTCCTGTGGCCGATCC
GTTCCGTCAGTGGGGACGTGATCGGCTTCGGCGCCCGCAAGCTCTTCGACGACGACCGTCTGGGCAAGTATCTGAACACG
CCGGAGACGATGCTGTACAAGAAGTCGAAGGTGCTGTTCGGCATTGACCAGGCGAAGCGGAACGTCGTGGCAAAGCACCA
GGCCGTGGTGGTGGAGGGCTACACGGATGTGATGGCTATGCACGCCGCCGGGGTGGATACGGCGGTGGCCGCGTGTGGCA
CGGCCTTCGGCGCGGAGCACTTGGCAGCCCTGCGGAGGTTTATGCTGGACGACCAGTTCTTCCGCGGGGAGATCATTTAC
ACCTTCGACGGTGATGAGGCCGGACAAAAGGCAGCGATGCGTGCCTTCGAGGGCGACCAACAGTTCTCGGGACGGAGCTA
TGTGACGGTCGCGCCGGGCGGGCAAGACCCGTGCGACGTGCGCATCAAGCGCGGCGATGCGGCCGTGCGCGAGCTGGTGG
CCTCCCGAATTCCGATGTTCGAGTTCGTGATCCGCACTCTCATCGCGGACTACGATACGGCCAGCATTGACGGGCGCGTG
CATGCTCTACGGCGCATCGTGCCGGTGCTGGCGGGGATTAAGGACGACGCGCTGCGGGATGAGTACGCCCGCCAGGCCGC
AGGTTGGGTCGGTTGGGATAACCCGAACGACGTGCTGGATCAGGTGCGCAAGGAGGCGCGGAAGTCCGGCGGCCAGCGGC
CGAAGCTGGAGTTGAAGACCGGCGCTGGTGCTGCTGGTGGCGCAGGAGGTTCTCGTGCTGGAGCGGCAGGCGCGGCGGGC
ACGGGCAGCGCAGGCGGCCCGAACCTGCACGTGGTCAAGGGCATGGAACGCCCGAACCCGAACGACGACTATCTGGAAGG
CCCCCGCGAAGTCATGAAGCTGGCTTTGCAGGAACCACAGCTGGCTTCCACCATCTTCGACCTCATGCCCCCGGAGAGTT
TCGTGCATCCGACATACGTGGCAATGGCTGAGGCTGTGGCGTCATTAGGAGGGGCGGCGAACAGCAACGGCGGGGCCGGG
TGGATCGAAAGAATCGCGGCACAGATGAAGGACCCGATGGGGGCGGCAGTGGTCTCCGAGCTGGCGGTGGAGGATCTGCA
TTGCCTGCCGGAGAGGCTGCCGTACTACGCGGATGCGATTATGGCGCGGATGCAGGAACGTTGGGTGGGCAGTGAGATCG
CGGACATGAAGTCCCGGATGCAGCGCATGCGCCCCGACCTGCAGAAAGAGGAGTATAACGCGCTGTTTGCGGATGTGATT
GCGTTGGAGAAGTACCGCCGCTCGCTGCACGAGCGGGCCTCGGACTTCGGCGACTTTACGCGCTAG

Upstream 100 bases:

>100_bases
TTAGCGCCCGGTTCTGTCGCGGTGTGTTGTTTGTAGAAAATAGGTTGCGGAGCAGGCCGATCTGGGACTTCCAGGGGCAG
TCCGGGGGTTGTACTAGCAT

Downstream 100 bases:

>100_bases
GAAAACCGTTTGGAGAACTACGCGCTAACGCTTTTTCTTAATTCAAACCCTGTAGCGCCACTCGATCGCGGTTCCATCGG
TCAGCATAAGTGGCCGAAGT

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 661; Mature: 660

Protein sequence:

>661_residues
MAMAKGRIPERDIEAIREQTPIEEVVGEYVQLKPGGVDSMKGLSPFKDEKTPSFHVRPNHGYFHCFSTDQGGDVFKFLML
MEHMSFPEAVEACAERIGYRISYEGGRPGNREEPGTRRRLVAANKAAFEFYRKEFLGDAEGADVARNFLIERGFTMEHAE
QFGCGYAPAGWDRLTKHLQQKGFTFKEIEAAGLGRMGKRGPIDRFHRRLLWPIRSVSGDVIGFGARKLFDDDRLGKYLNT
PETMLYKKSKVLFGIDQAKRNVVAKHQAVVVEGYTDVMAMHAAGVDTAVAACGTAFGAEHLAALRRFMLDDQFFRGEIIY
TFDGDEAGQKAAMRAFEGDQQFSGRSYVTVAPGGQDPCDVRIKRGDAAVRELVASRIPMFEFVIRTLIADYDTASIDGRV
HALRRIVPVLAGIKDDALRDEYARQAAGWVGWDNPNDVLDQVRKEARKSGGQRPKLELKTGAGAAGGAGGSRAGAAGAAG
TGSAGGPNLHVVKGMERPNPNDDYLEGPREVMKLALQEPQLASTIFDLMPPESFVHPTYVAMAEAVASLGGAANSNGGAG
WIERIAAQMKDPMGAAVVSELAVEDLHCLPERLPYYADAIMARMQERWVGSEIADMKSRMQRMRPDLQKEEYNALFADVI
ALEKYRRSLHERASDFGDFTR

Sequences:

>Translated_661_residues
MAMAKGRIPERDIEAIREQTPIEEVVGEYVQLKPGGVDSMKGLSPFKDEKTPSFHVRPNHGYFHCFSTDQGGDVFKFLML
MEHMSFPEAVEACAERIGYRISYEGGRPGNREEPGTRRRLVAANKAAFEFYRKEFLGDAEGADVARNFLIERGFTMEHAE
QFGCGYAPAGWDRLTKHLQQKGFTFKEIEAAGLGRMGKRGPIDRFHRRLLWPIRSVSGDVIGFGARKLFDDDRLGKYLNT
PETMLYKKSKVLFGIDQAKRNVVAKHQAVVVEGYTDVMAMHAAGVDTAVAACGTAFGAEHLAALRRFMLDDQFFRGEIIY
TFDGDEAGQKAAMRAFEGDQQFSGRSYVTVAPGGQDPCDVRIKRGDAAVRELVASRIPMFEFVIRTLIADYDTASIDGRV
HALRRIVPVLAGIKDDALRDEYARQAAGWVGWDNPNDVLDQVRKEARKSGGQRPKLELKTGAGAAGGAGGSRAGAAGAAG
TGSAGGPNLHVVKGMERPNPNDDYLEGPREVMKLALQEPQLASTIFDLMPPESFVHPTYVAMAEAVASLGGAANSNGGAG
WIERIAAQMKDPMGAAVVSELAVEDLHCLPERLPYYADAIMARMQERWVGSEIADMKSRMQRMRPDLQKEEYNALFADVI
ALEKYRRSLHERASDFGDFTR
>Mature_660_residues
AMAKGRIPERDIEAIREQTPIEEVVGEYVQLKPGGVDSMKGLSPFKDEKTPSFHVRPNHGYFHCFSTDQGGDVFKFLMLM
EHMSFPEAVEACAERIGYRISYEGGRPGNREEPGTRRRLVAANKAAFEFYRKEFLGDAEGADVARNFLIERGFTMEHAEQ
FGCGYAPAGWDRLTKHLQQKGFTFKEIEAAGLGRMGKRGPIDRFHRRLLWPIRSVSGDVIGFGARKLFDDDRLGKYLNTP
ETMLYKKSKVLFGIDQAKRNVVAKHQAVVVEGYTDVMAMHAAGVDTAVAACGTAFGAEHLAALRRFMLDDQFFRGEIIYT
FDGDEAGQKAAMRAFEGDQQFSGRSYVTVAPGGQDPCDVRIKRGDAAVRELVASRIPMFEFVIRTLIADYDTASIDGRVH
ALRRIVPVLAGIKDDALRDEYARQAAGWVGWDNPNDVLDQVRKEARKSGGQRPKLELKTGAGAAGGAGGSRAGAAGAAGT
GSAGGPNLHVVKGMERPNPNDDYLEGPREVMKLALQEPQLASTIFDLMPPESFVHPTYVAMAEAVASLGGAANSNGGAGW
IERIAAQMKDPMGAAVVSELAVEDLHCLPERLPYYADAIMARMQERWVGSEIADMKSRMQRMRPDLQKEEYNALFADVIA
LEKYRRSLHERASDFGDFTR

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=458, Percent_Identity=31.4410480349345, Blast_Score=208, Evalue=9e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013264
- InterPro:   IPR019475
- InterPro:   IPR006295
- InterPro:   IPR013173
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF10410 DnaB_bind; PF08278 DnaG_DnaB_bind; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.-

Molecular weight: Translated: 72893; Mature: 72761

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAMAKGRIPERDIEAIREQTPIEEVVGEYVQLKPGGVDSMKGLSPFKDEKTPSFHVRPNH
CCCCCCCCCHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEECCCC
GYFHCFSTDQGGDVFKFLMLMEHMSFPEAVEACAERIGYRISYEGGRPGNREEPGTRRRL
CEEEEECCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCEEEEECCCCCCCCCCCCHHHHH
VAANKAAFEFYRKEFLGDAEGADVARNFLIERGFTMEHAEQFGCGYAPAGWDRLTKHLQQ
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCHHHHHHCCCCCCCCCHHHHHHHHHH
KGFTFKEIEAAGLGRMGKRGPIDRFHRRLLWPIRSVSGDVIGFGARKLFDDDRLGKYLNT
CCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEECCHHHHCCHHHHHHHCCC
PETMLYKKSKVLFGIDQAKRNVVAKHQAVVVEGYTDVMAMHAAGVDTAVAACGTAFGAEH
CHHHHHHHCHHEEEHHHHHHHHHHHHHEEEEECHHHHHHHHHCCHHHHHHHHHHHHHHHH
LAALRRFMLDDQFFRGEIIYTFDGDEAGQKAAMRAFEGDQQFSGRSYVTVAPGGQDPCDV
HHHHHHHHHCCHHHCCEEEEEECCCHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCE
RIKRGDAAVRELVASRIPMFEFVIRTLIADYDTASIDGRVHALRRIVPVLAGIKDDALRD
EEECCHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHH
EYARQAAGWVGWDNPNDVLDQVRKEARKSGGQRPKLELKTGAGAAGGAGGSRAGAAGAAG
HHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCC
TGSAGGPNLHVVKGMERPNPNDDYLEGPREVMKLALQEPQLASTIFDLMPPESFVHPTYV
CCCCCCCCEEEEECCCCCCCCCHHHCCHHHHHHHHHCCCHHHHHHHHHCCCHHHCCHHHH
AMAEAVASLGGAANSNGGAGWIERIAAQMKDPMGAAVVSELAVEDLHCLPERLPYYADAI
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHH
MARMQERWVGSEIADMKSRMQRMRPDLQKEEYNALFADVIALEKYRRSLHERASDFGDFT
HHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
R
C
>Mature Secondary Structure 
AMAKGRIPERDIEAIREQTPIEEVVGEYVQLKPGGVDSMKGLSPFKDEKTPSFHVRPNH
CCCCCCCCHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEECCCC
GYFHCFSTDQGGDVFKFLMLMEHMSFPEAVEACAERIGYRISYEGGRPGNREEPGTRRRL
CEEEEECCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCEEEEECCCCCCCCCCCCHHHHH
VAANKAAFEFYRKEFLGDAEGADVARNFLIERGFTMEHAEQFGCGYAPAGWDRLTKHLQQ
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCHHHHHHCCCCCCCCCHHHHHHHHHH
KGFTFKEIEAAGLGRMGKRGPIDRFHRRLLWPIRSVSGDVIGFGARKLFDDDRLGKYLNT
CCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEECCHHHHCCHHHHHHHCCC
PETMLYKKSKVLFGIDQAKRNVVAKHQAVVVEGYTDVMAMHAAGVDTAVAACGTAFGAEH
CHHHHHHHCHHEEEHHHHHHHHHHHHHEEEEECHHHHHHHHHCCHHHHHHHHHHHHHHHH
LAALRRFMLDDQFFRGEIIYTFDGDEAGQKAAMRAFEGDQQFSGRSYVTVAPGGQDPCDV
HHHHHHHHHCCHHHCCEEEEEECCCHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCE
RIKRGDAAVRELVASRIPMFEFVIRTLIADYDTASIDGRVHALRRIVPVLAGIKDDALRD
EEECCHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHH
EYARQAAGWVGWDNPNDVLDQVRKEARKSGGQRPKLELKTGAGAAGGAGGSRAGAAGAAG
HHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCC
TGSAGGPNLHVVKGMERPNPNDDYLEGPREVMKLALQEPQLASTIFDLMPPESFVHPTYV
CCCCCCCCEEEEECCCCCCCCCHHHCCHHHHHHHHHCCCHHHHHHHHHCCCHHHCCHHHH
AMAEAVASLGGAANSNGGAGWIERIAAQMKDPMGAAVVSELAVEDLHCLPERLPYYADAI
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHH
MARMQERWVGSEIADMKSRMQRMRPDLQKEEYNALFADVIALEKYRRSLHERASDFGDFT
HHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
R
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9422594 [H]