Click here to switch to the map view.

The map label for this gene is pitB [C]

Identifier: 68535688

GI number: 68535688

Start: 743093

End: 744718

Strand: Direct

Name: pitB [C]

Synonym: jk0616

Alternate gene names: 68535688

Gene position: 743093-744718 (Clockwise)

Preceding gene: 68535686

Following gene: 68535690

Centisome position: 30.18

GC content: 58.06

Gene sequence:

>1626_bases
TTGTCGACGACAACCGAAACGGCCGCGCCATCGTCGGCCGCGGGCATCACCCCTGTGCCCGAAAATGAGAATAATACGTG
GTGGCACCTGTTTTTCGGCGGTCTTATGGCCGTCACCTTGGTGACATTCAGTCTGTGGGCTCACGACTACGTTGGTGAAA
ACGCCAGCTTGATTGTTCTGCTGACCACCATCATTTTCGCCGTGTTCATGGCGTTCAACATCGGTGGTAACGACGTTGCG
AACTCCTTCGGTACCTCCGTGGGCGCCGGCACGCTGAGTCTGAAGCAGGCTCTGGTTGTAGCCGCTATCTTCGAGGTTTC
CGGTGCCGTGCTGGCTGGTGGCGAGGTTACGGATACGGTCCGTTCCGGCATCGTTGACCTGGGTGCTATCGACAACCTCG
AGCCGATGAACTTTGCCTACATCATGATGGCCTCCCTGCTGGGTGCGGCCGTGTGGCTGCTGGTGGCGACCCGCATGGGC
TGGCCGGTGTCCACCACGCACTCCATCGTGGGCGGCATCGTTGGTGCGGCTTTGACCGTCGGCTTCATCACCGGCACTGG
CGGCTGGTCCATGGTCCAGTGGGGCGAGGTTGGCAAGATCGCCATTTCTTGGGTTCTGTCTCCGGCACTGGGCGGCATCG
TTGCATACCTTCTTTATGGCGCGATTAAGCGTGGCATCTTGGTCTACAACGAGCTGGCGGACCAGCGCCTCGATGAGATC
AAGAAGGAAAAGAAGGAGCTGAACATTCAGCACAAGATCGCCTTCGGTCAGCTCAGCGAGGAAGAGCAGCTGGCTCTGAC
CGATGCGATGATTCGTGACTCGACAACGATGCGCGAGCACGACTTCACTGAGGAGGACCTGGAGTCCGATTACTACAAGA
AGCTGCACCGCATCAATAAGTCCATTGAGGACGTTGAGGCGCACCACGCTCTGGATACCTGGGTGCCGATGCTGGCCGCA
GTTGGTTCCATCATCATCTCCGCGATGCTGCTGTTCAAGGGTCTGAAGAACATGAACCTGGAGCTGTCCAGCCTGGGCAA
CTTCCTGATCATGGGCATGGTGGCAGCCGTTGTTTGGATGGCTGTTTACATCTTCTCCCGCACCCTGAAGAAGCAGGCGC
TAAACCGCTCGACCTTCCTGCTGTTCAGCTGGATGCAGGTGTTCACCGCATCTGCATTCGCATTCAGCCACGGCTCCAAC
GACATCGCTAACGCGCTTGGACCGTTCGTTGCAGTGCTGGATGTGTTGAAGACGAACTCCATCTCTGAGGAGTCCGCTGT
GGCTCTGCCGGTCATGGTTGCCATGGGTGTTGCCTTGATTGCTGGCCTGTGGTTCATTGGCCGCTTCGTTATCCGCACCG
TTGGTTCCGGCCTGACCAAGATGCACCCGGCCTCTGGCTTTGCTGCAGAGCTGTCTGCAGCTGCCGTGGTTATGGGCGCA
TCCGTGCTGGGTCTGCCGGTTTCCTCCACCCACATTCTGATTGGTGCTGTTCTGGGCGTTGGCCTGGTTAATAAGGATGC
CAATTGGGGTCTGATGAAGCCCATCGCCCTGGCTTGGGTTATCACTCTGCCGGCTGCTGCGATCATCTCCAGTGGTACCG
TCGCGGTTCTTCGCGTGATCTTCTAA

Upstream 100 bases:

>100_bases
TTCAACCTTCGTTAACTTTCTGTTCAACATTAAAACTCGTTCTTTTTACCTCCATCGGACAAGGTGGCTCTGTCTGACAT
CTTGACGTTTTGGAGGAATC

Downstream 100 bases:

>100_bases
AGCTAACTGCTTGCCGCTGGCTTACCTCGGCTGGCTGGCTTGCTACTGCAGGCTCGGCCTGGCTGAGGTTGCTAGCCCGG
CCAGCTGACCTAAGGCGCTC

Product: putative phosphate permease

Products: Proton [Cytoplasm]; phosphate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 541; Mature: 540

Protein sequence:

>541_residues
MSTTTETAAPSSAAGITPVPENENNTWWHLFFGGLMAVTLVTFSLWAHDYVGENASLIVLLTTIIFAVFMAFNIGGNDVA
NSFGTSVGAGTLSLKQALVVAAIFEVSGAVLAGGEVTDTVRSGIVDLGAIDNLEPMNFAYIMMASLLGAAVWLLVATRMG
WPVSTTHSIVGGIVGAALTVGFITGTGGWSMVQWGEVGKIAISWVLSPALGGIVAYLLYGAIKRGILVYNELADQRLDEI
KKEKKELNIQHKIAFGQLSEEEQLALTDAMIRDSTTMREHDFTEEDLESDYYKKLHRINKSIEDVEAHHALDTWVPMLAA
VGSIIISAMLLFKGLKNMNLELSSLGNFLIMGMVAAVVWMAVYIFSRTLKKQALNRSTFLLFSWMQVFTASAFAFSHGSN
DIANALGPFVAVLDVLKTNSISEESAVALPVMVAMGVALIAGLWFIGRFVIRTVGSGLTKMHPASGFAAELSAAAVVMGA
SVLGLPVSSTHILIGAVLGVGLVNKDANWGLMKPIALAWVITLPAAAIISSGTVAVLRVIF

Sequences:

>Translated_541_residues
MSTTTETAAPSSAAGITPVPENENNTWWHLFFGGLMAVTLVTFSLWAHDYVGENASLIVLLTTIIFAVFMAFNIGGNDVA
NSFGTSVGAGTLSLKQALVVAAIFEVSGAVLAGGEVTDTVRSGIVDLGAIDNLEPMNFAYIMMASLLGAAVWLLVATRMG
WPVSTTHSIVGGIVGAALTVGFITGTGGWSMVQWGEVGKIAISWVLSPALGGIVAYLLYGAIKRGILVYNELADQRLDEI
KKEKKELNIQHKIAFGQLSEEEQLALTDAMIRDSTTMREHDFTEEDLESDYYKKLHRINKSIEDVEAHHALDTWVPMLAA
VGSIIISAMLLFKGLKNMNLELSSLGNFLIMGMVAAVVWMAVYIFSRTLKKQALNRSTFLLFSWMQVFTASAFAFSHGSN
DIANALGPFVAVLDVLKTNSISEESAVALPVMVAMGVALIAGLWFIGRFVIRTVGSGLTKMHPASGFAAELSAAAVVMGA
SVLGLPVSSTHILIGAVLGVGLVNKDANWGLMKPIALAWVITLPAAAIISSGTVAVLRVIF
>Mature_540_residues
STTTETAAPSSAAGITPVPENENNTWWHLFFGGLMAVTLVTFSLWAHDYVGENASLIVLLTTIIFAVFMAFNIGGNDVAN
SFGTSVGAGTLSLKQALVVAAIFEVSGAVLAGGEVTDTVRSGIVDLGAIDNLEPMNFAYIMMASLLGAAVWLLVATRMGW
PVSTTHSIVGGIVGAALTVGFITGTGGWSMVQWGEVGKIAISWVLSPALGGIVAYLLYGAIKRGILVYNELADQRLDEIK
KEKKELNIQHKIAFGQLSEEEQLALTDAMIRDSTTMREHDFTEEDLESDYYKKLHRINKSIEDVEAHHALDTWVPMLAAV
GSIIISAMLLFKGLKNMNLELSSLGNFLIMGMVAAVVWMAVYIFSRTLKKQALNRSTFLLFSWMQVFTASAFAFSHGSND
IANALGPFVAVLDVLKTNSISEESAVALPVMVAMGVALIAGLWFIGRFVIRTVGSGLTKMHPASGFAAELSAAAVVMGAS
VLGLPVSSTHILIGAVLGVGLVNKDANWGLMKPIALAWVITLPAAAIISSGTVAVLRVIF

Specific function: Potential transporter for phosphate [H]

COG id: COG0306

COG function: function code P; Phosphate/sulphate permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the inorganic phosphate transporter (PiT) (TC 2.A.20) family [H]

Homologues:

Organism=Homo sapiens, GI31543630, Length=163, Percent_Identity=41.7177914110429, Blast_Score=131, Evalue=1e-30,
Organism=Homo sapiens, GI5803173, Length=160, Percent_Identity=41.875, Blast_Score=128, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI25146401, Length=497, Percent_Identity=33.8028169014084, Blast_Score=240, Evalue=1e-63,
Organism=Caenorhabditis elegans, GI71981576, Length=178, Percent_Identity=37.0786516853933, Blast_Score=141, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI17539280, Length=177, Percent_Identity=40.1129943502825, Blast_Score=128, Evalue=7e-30,
Organism=Caenorhabditis elegans, GI32566716, Length=168, Percent_Identity=43.452380952381, Blast_Score=124, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17536725, Length=164, Percent_Identity=31.7073170731707, Blast_Score=102, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI17557328, Length=221, Percent_Identity=30.316742081448, Blast_Score=101, Evalue=9e-22,
Organism=Saccharomyces cerevisiae, GI6319773, Length=159, Percent_Identity=38.9937106918239, Blast_Score=117, Evalue=5e-27,
Organism=Drosophila melanogaster, GI21356511, Length=167, Percent_Identity=47.3053892215569, Blast_Score=137, Evalue=2e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001204 [H]

Pfam domain/function: PF01384 PHO4 [H]

EC number: NA

Molecular weight: Translated: 57531; Mature: 57400

Theoretical pI: Translated: 5.58; Mature: 5.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTTTETAAPSSAAGITPVPENENNTWWHLFFGGLMAVTLVTFSLWAHDYVGENASLIVL
CCCCCCCCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
LTTIIFAVFMAFNIGGNDVANSFGTSVGAGTLSLKQALVVAAIFEVSGAVLAGGEVTDTV
HHHHHHHHHHHHCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCEEECCCHHHHH
RSGIVDLGAIDNLEPMNFAYIMMASLLGAAVWLLVATRMGWPVSTTHSIVGGIVGAALTV
HHCCCHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
GFITGTGGWSMVQWGEVGKIAISWVLSPALGGIVAYLLYGAIKRGILVYNELADQRLDEI
HHEECCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KKEKKELNIQHKIAFGQLSEEEQLALTDAMIRDSTTMREHDFTEEDLESDYYKKLHRINK
HHHHHHCCHHHEEEECCCCCCHHHHHHHHHHHCCCCHHHCCCCHHHHHHHHHHHHHHHCC
SIEDVEAHHALDTWVPMLAAVGSIIISAMLLFKGLKNMNLELSSLGNFLIMGMVAAVVWM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHH
AVYIFSRTLKKQALNRSTFLLFSWMQVFTASAFAFSHGSNDIANALGPFVAVLDVLKTNS
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCC
ISEESAVALPVMVAMGVALIAGLWFIGRFVIRTVGSGLTKMHPASGFAAELSAAAVVMGA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCHHHHHHHHHHHHHH
SVLGLPVSSTHILIGAVLGVGLVNKDANWGLMKPIALAWVITLPAAAIISSGTVAVLRVI
HHHCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
F
C
>Mature Secondary Structure 
STTTETAAPSSAAGITPVPENENNTWWHLFFGGLMAVTLVTFSLWAHDYVGENASLIVL
CCCCCCCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
LTTIIFAVFMAFNIGGNDVANSFGTSVGAGTLSLKQALVVAAIFEVSGAVLAGGEVTDTV
HHHHHHHHHHHHCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCEEECCCHHHHH
RSGIVDLGAIDNLEPMNFAYIMMASLLGAAVWLLVATRMGWPVSTTHSIVGGIVGAALTV
HHCCCHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
GFITGTGGWSMVQWGEVGKIAISWVLSPALGGIVAYLLYGAIKRGILVYNELADQRLDEI
HHEECCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KKEKKELNIQHKIAFGQLSEEEQLALTDAMIRDSTTMREHDFTEEDLESDYYKKLHRINK
HHHHHHCCHHHEEEECCCCCCHHHHHHHHHHHCCCCHHHCCCCHHHHHHHHHHHHHHHCC
SIEDVEAHHALDTWVPMLAAVGSIIISAMLLFKGLKNMNLELSSLGNFLIMGMVAAVVWM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHH
AVYIFSRTLKKQALNRSTFLLFSWMQVFTASAFAFSHGSNDIANALGPFVAVLDVLKTNS
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCC
ISEESAVALPVMVAMGVALIAGLWFIGRFVIRTVGSGLTKMHPASGFAAELSAAAVVMGA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCHHHHHHHHHHHHHH
SVLGLPVSSTHILIGAVLGVGLVNKDANWGLMKPIALAWVITLPAAAIISSGTVAVLRVI
HHHCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
F
C

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; phosphate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + phosphate [Periplasm] = Proton [Cytoplasm] + phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]