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The map label for this gene is ape2 [H]

Identifier: 66766941

GI number: 66766941

Start: 718875

End: 720230

Strand: Reverse

Name: ape2 [H]

Synonym: XC_0602

Alternate gene names: 66766941

Gene position: 720230-718875 (Counterclockwise)

Preceding gene: 66766942

Following gene: 66766940

Centisome position: 13.99

GC content: 62.83

Gene sequence:

>1356_bases
GTGACACATCGGGGACTCACTGCATACAAGGTCCTGCTTGCACTGTTGGCACTGCTGGCACTTGCACCGACTTCGCTGAT
GGCCGCAGCACCGACACTGCCAGGCGCGGGGTTCGAGGTCGAGGGCTATCAGCTGCAACTGCGCCCGGACCTCAGCACCA
CCGCGCTTTCCGGCGTGCAGCGCATTCGCCTACGCAGCACCTCCGACCACCTCACCCAGCTGACGTTCTCGCCTAATGCA
CTGCAGATCCTCTCGGCCGAACTAAATGGTCAGCCGATCGCAGTGACCAGCACCACCGATGGCATCACGTTTGCGTTGCC
AAGCGCGCTTGCCAAAGGCGCCCGCGCGTCGCTCGCCTTCAAGATCGTCGGCGTGCCGGCCCAGGGTGTTGTTCGCGCGG
CCAGTGGCTTCTACACCAGCTACTTCGCGTGCGACTGGATGGTCTGCCTGCAGGACAGCCCCGGCCAAAAAGCGTCTTTC
GCTTTGGATCTGTTCGTGCCTGCCGGCATCGATACGTTGAGCATCGGCGCAGGCTTGCCCCAGAAGGTGCTTCCGAACGG
CCTGGTGCTGCACCGCTGGCGCGCTACCAGGCCGTATTCGGCCTATCTGTTCGGCTTTGCGGTTGGCACCTTCTCGCAGC
AGTCATCCAAAACCACGGCCGGGACGTTCGTCTATCTCGATGGCACAGGTCGAGGTGCGGACCTGGCAGACGCATTCCAG
CAGACACCCTCCATTGCGGCATTTTTTGCGCAGGCGGCAGGCGTATCGCTCCCGGATGGTCGTTACACCCAGCTGCTTGT
GCCCGGACAGGAGGCGCAGGAGGCGGCCACCTTCTCGCTCATTGGCGTGCAAGCACTGCAAGACGAGCAGGAAGACCCCG
CTTCGTCATGGATCATCGCGCATGAAATGGCGCATCAGTGGTGGGGAAACCTTGTCACCTGCGCATCGTGGCAAGACTTC
TGGTTGAACGAGGGCATCACCACGTTCATGGTCGCCGCCTGGAAGCAACACGCCTTCGGCGAGGCGGCCTACCAGCAGGA
GTTGAATGAAGCACGCCGCCGGCTTGCCATCGCCCGTGACGCCGGCTTCGACAAACCGCTGGCCTGGTCAGGTGAGTACC
CCTCGTTACGTGTCCGCCGCGCCGTGCAGTACAGCAAAGGCGCGCTGTTCATGGCGCACTTGCGGCAGACGCTGGGTGAG
CAGGCGTTTTGGGCGGGTATGCGCCGTTACACCCGCAAGAACGTGGGCAGCACCGTCGTCAGTCAGGACCTTCAGGGAGC
AATGGAGCACGCCAGCGGGCGCGATCTTTCAGCACTGTTCGCGCAGTGGGTGTATGGCGACGAAGCGACTAACTGA

Upstream 100 bases:

>100_bases
ACGTTCTCAATGACGAGGACCACCTGACCATCGGGCCACGCGGCACCACCCACGGCTTGAAGGTCTTGCTCGGGATCAAG
ACGGCTGATCGGAGCGAGCG

Downstream 100 bases:

>100_bases
CCCCGGCAAGCCAAAACCATCAAGACCACATGCTCCGCAGCATGGCCATGCGCGGAACAGCGTAGCGCTGCTGCGTAAGA
GTGCGTCTTGATCGTTGCAT

Product: aminopeptidase N

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 451; Mature: 450

Protein sequence:

>451_residues
MTHRGLTAYKVLLALLALLALAPTSLMAAAPTLPGAGFEVEGYQLQLRPDLSTTALSGVQRIRLRSTSDHLTQLTFSPNA
LQILSAELNGQPIAVTSTTDGITFALPSALAKGARASLAFKIVGVPAQGVVRAASGFYTSYFACDWMVCLQDSPGQKASF
ALDLFVPAGIDTLSIGAGLPQKVLPNGLVLHRWRATRPYSAYLFGFAVGTFSQQSSKTTAGTFVYLDGTGRGADLADAFQ
QTPSIAAFFAQAAGVSLPDGRYTQLLVPGQEAQEAATFSLIGVQALQDEQEDPASSWIIAHEMAHQWWGNLVTCASWQDF
WLNEGITTFMVAAWKQHAFGEAAYQQELNEARRRLAIARDAGFDKPLAWSGEYPSLRVRRAVQYSKGALFMAHLRQTLGE
QAFWAGMRRYTRKNVGSTVVSQDLQGAMEHASGRDLSALFAQWVYGDEATN

Sequences:

>Translated_451_residues
MTHRGLTAYKVLLALLALLALAPTSLMAAAPTLPGAGFEVEGYQLQLRPDLSTTALSGVQRIRLRSTSDHLTQLTFSPNA
LQILSAELNGQPIAVTSTTDGITFALPSALAKGARASLAFKIVGVPAQGVVRAASGFYTSYFACDWMVCLQDSPGQKASF
ALDLFVPAGIDTLSIGAGLPQKVLPNGLVLHRWRATRPYSAYLFGFAVGTFSQQSSKTTAGTFVYLDGTGRGADLADAFQ
QTPSIAAFFAQAAGVSLPDGRYTQLLVPGQEAQEAATFSLIGVQALQDEQEDPASSWIIAHEMAHQWWGNLVTCASWQDF
WLNEGITTFMVAAWKQHAFGEAAYQQELNEARRRLAIARDAGFDKPLAWSGEYPSLRVRRAVQYSKGALFMAHLRQTLGE
QAFWAGMRRYTRKNVGSTVVSQDLQGAMEHASGRDLSALFAQWVYGDEATN
>Mature_450_residues
THRGLTAYKVLLALLALLALAPTSLMAAAPTLPGAGFEVEGYQLQLRPDLSTTALSGVQRIRLRSTSDHLTQLTFSPNAL
QILSAELNGQPIAVTSTTDGITFALPSALAKGARASLAFKIVGVPAQGVVRAASGFYTSYFACDWMVCLQDSPGQKASFA
LDLFVPAGIDTLSIGAGLPQKVLPNGLVLHRWRATRPYSAYLFGFAVGTFSQQSSKTTAGTFVYLDGTGRGADLADAFQQ
TPSIAAFFAQAAGVSLPDGRYTQLLVPGQEAQEAATFSLIGVQALQDEQEDPASSWIIAHEMAHQWWGNLVTCASWQDFW
LNEGITTFMVAAWKQHAFGEAAYQQELNEARRRLAIARDAGFDKPLAWSGEYPSLRVRRAVQYSKGALFMAHLRQTLGEQ
AFWAGMRRYTRKNVGSTVVSQDLQGAMEHASGRDLSALFAQWVYGDEATN

Specific function: Preferentially acts as a leucyl-aminopeptidase, although it also has activity against other substrates [H]

COG id: COG0308

COG function: function code E; Aminopeptidase N

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M1 family [H]

Homologues:

Organism=Homo sapiens, GI158937236, Length=443, Percent_Identity=24.6049661399549, Blast_Score=103, Evalue=4e-22,
Organism=Homo sapiens, GI61742775, Length=447, Percent_Identity=24.1610738255034, Blast_Score=99, Evalue=6e-21,
Organism=Homo sapiens, GI61742777, Length=447, Percent_Identity=24.1610738255034, Blast_Score=99, Evalue=6e-21,
Organism=Homo sapiens, GI310133497, Length=276, Percent_Identity=27.536231884058, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI310123622, Length=276, Percent_Identity=27.536231884058, Blast_Score=86, Evalue=7e-17,
Organism=Homo sapiens, GI194306629, Length=291, Percent_Identity=24.3986254295533, Blast_Score=85, Evalue=1e-16,
Organism=Homo sapiens, GI11641261, Length=291, Percent_Identity=24.3986254295533, Blast_Score=85, Evalue=1e-16,
Organism=Homo sapiens, GI94818901, Length=295, Percent_Identity=26.4406779661017, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI94818891, Length=295, Percent_Identity=26.4406779661017, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI132814467, Length=307, Percent_Identity=23.4527687296417, Blast_Score=76, Evalue=7e-14,
Organism=Homo sapiens, GI157266300, Length=288, Percent_Identity=27.7777777777778, Blast_Score=76, Evalue=7e-14,
Organism=Homo sapiens, GI4505029, Length=432, Percent_Identity=22.6851851851852, Blast_Score=74, Evalue=3e-13,
Organism=Escherichia coli, GI1787163, Length=153, Percent_Identity=33.3333333333333, Blast_Score=69, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI17565628, Length=466, Percent_Identity=22.9613733905579, Blast_Score=86, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI115533276, Length=444, Percent_Identity=23.4234234234234, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI115533278, Length=444, Percent_Identity=23.1981981981982, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI71989071, Length=445, Percent_Identity=23.3707865168539, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI71989076, Length=445, Percent_Identity=23.3707865168539, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI193206928, Length=346, Percent_Identity=24.5664739884393, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI193206926, Length=346, Percent_Identity=24.5664739884393, Blast_Score=76, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI71984339, Length=303, Percent_Identity=26.0726072607261, Blast_Score=75, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI71984353, Length=303, Percent_Identity=26.0726072607261, Blast_Score=75, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI71990873, Length=277, Percent_Identity=26.7148014440433, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17569225, Length=433, Percent_Identity=22.4018475750577, Blast_Score=72, Evalue=6e-13,
Organism=Saccharomyces cerevisiae, GI9755335, Length=445, Percent_Identity=24.4943820224719, Blast_Score=102, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6321837, Length=449, Percent_Identity=22.71714922049, Blast_Score=78, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24655274, Length=444, Percent_Identity=25.2252252252252, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24655260, Length=444, Percent_Identity=25.2252252252252, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24655265, Length=444, Percent_Identity=25.2252252252252, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24655268, Length=444, Percent_Identity=25.2252252252252, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24655252, Length=444, Percent_Identity=25.2252252252252, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24655257, Length=444, Percent_Identity=25.2252252252252, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24646516, Length=484, Percent_Identity=24.5867768595041, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24646518, Length=482, Percent_Identity=24.6887966804979, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24651016, Length=276, Percent_Identity=26.4492753623188, Blast_Score=92, Evalue=6e-19,
Organism=Drosophila melanogaster, GI24646514, Length=451, Percent_Identity=24.6119733924612, Blast_Score=92, Evalue=7e-19,
Organism=Drosophila melanogaster, GI21358341, Length=281, Percent_Identity=27.0462633451957, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24651025, Length=282, Percent_Identity=25.531914893617, Blast_Score=83, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24651023, Length=282, Percent_Identity=25.531914893617, Blast_Score=83, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24651021, Length=282, Percent_Identity=25.531914893617, Blast_Score=83, Evalue=4e-16,
Organism=Drosophila melanogaster, GI221379089, Length=439, Percent_Identity=22.7790432801822, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24650973, Length=436, Percent_Identity=22.0183486238532, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI45550850, Length=436, Percent_Identity=22.0183486238532, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI221330574, Length=261, Percent_Identity=25.2873563218391, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI161078673, Length=159, Percent_Identity=32.0754716981132, Blast_Score=69, Evalue=5e-12,
Organism=Drosophila melanogaster, GI28571901, Length=159, Percent_Identity=32.0754716981132, Blast_Score=69, Evalue=6e-12,
Organism=Drosophila melanogaster, GI24648790, Length=166, Percent_Identity=27.710843373494, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001930
- InterPro:   IPR014782 [H]

Pfam domain/function: PF01433 Peptidase_M1 [H]

EC number: =3.4.11.1 [H]

Molecular weight: Translated: 48742; Mature: 48611

Theoretical pI: Translated: 7.25; Mature: 7.25

Prosite motif: PS00142 ZINC_PROTEASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHRGLTAYKVLLALLALLALAPTSLMAAAPTLPGAGFEVEGYQLQLRPDLSTTALSGVQ
CCCCCHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCEEECCEEEEECCCCCHHHHHHHH
RIRLRSTSDHLTQLTFSPNALQILSAELNGQPIAVTSTTDGITFALPSALAKGARASLAF
HHHCCCCCCHHEEEEECCCCEEEEEECCCCCCEEEEECCCCEEEECHHHHHCCCCCEEEE
KIVGVPAQGVVRAASGFYTSYFACDWMVCLQDSPGQKASFALDLFVPAGIDTLSIGAGLP
EEEECCCHHHHHHHCCHHHHHHHHEEEEEECCCCCCCCEEEEEEEECCCCCEEECCCCCC
QKVLPNGLVLHRWRATRPYSAYLFGFAVGTFSQQSSKTTAGTFVYLDGTGRGADLADAFQ
HHHCCCCEEEEEEECCCCCHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCHHHHHHH
QTPSIAAFFAQAAGVSLPDGRYTQLLVPGQEAQEAATFSLIGVQALQDEQEDPASSWIIA
CCCHHHHHHHHHCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
HEMAHQWWGNLVTCASWQDFWLNEGITTFMVAAWKQHAFGEAAYQQELNEARRRLAIARD
HHHHHHHHCCEEEECCCHHHHHHCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHC
AGFDKPLAWSGEYPSLRVRRAVQYSKGALFMAHLRQTLGEQAFWAGMRRYTRKNVGSTVV
CCCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SQDLQGAMEHASGRDLSALFAQWVYGDEATN
HHHHHHHHHHCCCCCHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
THRGLTAYKVLLALLALLALAPTSLMAAAPTLPGAGFEVEGYQLQLRPDLSTTALSGVQ
CCCCHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCEEECCEEEEECCCCCHHHHHHHH
RIRLRSTSDHLTQLTFSPNALQILSAELNGQPIAVTSTTDGITFALPSALAKGARASLAF
HHHCCCCCCHHEEEEECCCCEEEEEECCCCCCEEEEECCCCEEEECHHHHHCCCCCEEEE
KIVGVPAQGVVRAASGFYTSYFACDWMVCLQDSPGQKASFALDLFVPAGIDTLSIGAGLP
EEEECCCHHHHHHHCCHHHHHHHHEEEEEECCCCCCCCEEEEEEEECCCCCEEECCCCCC
QKVLPNGLVLHRWRATRPYSAYLFGFAVGTFSQQSSKTTAGTFVYLDGTGRGADLADAFQ
HHHCCCCEEEEEEECCCCCHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCHHHHHHH
QTPSIAAFFAQAAGVSLPDGRYTQLLVPGQEAQEAATFSLIGVQALQDEQEDPASSWIIA
CCCHHHHHHHHHCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
HEMAHQWWGNLVTCASWQDFWLNEGITTFMVAAWKQHAFGEAAYQQELNEARRRLAIARD
HHHHHHHHCCEEEECCCHHHHHHCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHC
AGFDKPLAWSGEYPSLRVRRAVQYSKGALFMAHLRQTLGEQAFWAGMRRYTRKNVGSTVV
CCCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SQDLQGAMEHASGRDLSALFAQWVYGDEATN
HHHHHHHHHHCCCCCHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9723917; 8899719; 11427726 [H]