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The map label for this gene is gacS [H]

Identifier: 66766834

GI number: 66766834

Start: 582841

End: 585144

Strand: Reverse

Name: gacS [H]

Synonym: XC_0495

Alternate gene names: 66766834

Gene position: 585144-582841 (Counterclockwise)

Preceding gene: 77761089

Following gene: 66766828

Centisome position: 11.36

GC content: 68.53

Gene sequence:

>2304_bases
GTGGCGGCGCGGGCAGGAGGCCTTCGCGGCTGGAGCACGCTGGTCGCCTTGGCCGCACTGGTTGCAGCATCCGCTGCGGC
GTTGCAGTGGCTTGGCGTCGCAGGGCCATGGCGGATCGTCACCACCGCTGCCAGCGTGCTCGCGCTGGTTTGCGGCATCT
GTACCGCTGTTGCTGCTTATCGCGCCAGCCGCACGCTGAGCGCACTCACCCGCCAGCTCGACGACACCAACACCGCGCAG
GCGCGCATGCAGCAGGAGTTGCAGCGCCACGGCGAACTGGAACAGGAACTGCTGCGCGCCAAGCAGGCCGCCGAAGCCGC
CTCGCTGGCCAAGGGCGAATTTCTGGCCACCATGAGCCATGAGATCCGCACCCCGCTCAACGGCATCATCCCGATGCTGG
AGCTGATCTCGCGTGGCCAGCTCAGCCTGGACCAACGCGACATGCTGCAGACCGCCACCGGCAGCTCGCTGCAGCTGCTG
CGCATCGTCGACGACATCCTGGATTACTCCAAGCTCGAAGCGAACAAGCTCGAACTGGAAATCACCACCTTCAACCTGCG
CGAGCTGCTCGACGGTGTCGCCCAGCTGATGCAGCGCACGGCCGAAGGCAAGCAACTGCGCCTGGGCCTGGACATCGAAC
CGTCGGTGCGCCTGCTGGTGCGCGGCGACCCGATCCGGCTACGCCAGGTGCTGGGCAACCTGATCGGCAACGCCATCAAG
TTCACCGAACGCGGCAGCGTGGACATCCAGCTGCGGCGCCTGGGCGAAACCCGCGCCCAGCACCTGCTGCGCTTCCAGGT
CCGCGACACCGGCATCGGCATCGCAGCGGACCAGCAGGCGCGCCTGTTCCGCTCCTTCGCCCAGGCCGATGCCTCCACTA
CCCGCCTGTATGGCGGCACCGGGCTGGGCCTGGCCATCTGCAAACGCATCATCGACCTGATGGGCGGCCGCATCGGCGTG
GAATCGGAGCCCGGTCGCGGCGCCACGTTCTGGTTCGAAATCCCGCTGCTCAAGGTCATCGGCGACCTGCAACAGACCAC
CGGCGCCGACGCCGCACGCGTGATGGTGATCAGTTCCGACGCGCGCCTGTCGCAGCGGCTCAAGCGCCTGCTCGACAGCT
GGGGCATCTCGCACGTGCTGATGGAAACCACCCAGGAAGCGCTGGAGCGCGTGCGCCGCCACACCGACGACGAAGGCTTC
CGCTGCGTCATCGCCGACCACGAGACGCTGCGCTACAGCGCGCGCGCCGTGCATCGCGCCCTGGCACGGCCGGAAGACCT
CAGCGGCTCACGGCTGATCTGGCTCTACGGCGACGAGCCGGTACCCGACGAACTGCAAGCCAACGCCACCCTGGTCCCGC
GCCAGAGCCCGGACGAAACCCTGCGCGCGCTGGTACTGCCACCCGAGCCCAAGCCATCTGTGGCGCCCACGCTGGCGTCC
GCGATGATCCCCGAGCCACTGCCGCCGCCAGTGGCGCATGCGCGCGACGTGCGCATCCTGTTGGTCGAAGACAACCCGGT
CAACCTGCTGGTCGCGCAAAAACTGCTGGGCGTGCTCGGCTTCGAGGCCGACACCGCCACCGATGGCGAAGCCGCGCTGA
GCAGCATGGAGTCCACCCGCTACGACATGGTTTTCATGGATTGTCAGATGCCGGTGCTGGATGGCTATGCCGCCACCCGC
CGCTGGCGCGCCATGGAAACCGAAAGCGGTGGCCGCCCGATCCCGATCGTGGCGATGACCGCCAACGCCATGGCCGGCGA
CCGCGAACGCTGTCTGGCCGCGGGCATGGACGACTATCTCTCCAAGCCGGTCGCCCGCGAACAGCTCAATGCCTGCCTGC
AGCGCTGGCTGCCGCGCCAGGCCCTGTTGCCAGGCCCAAGCACCGGAGCCCCCAGCGCGGATGCCGAGAGCGCTGCCAGC
GCCGCACAGGCGCGCGCGCTGCCGATCCTGGAGAGCGGCGTCATCGACGAGTTGTACGAGGTGGCAGGCGCGGACACCAT
CCGCATCCTGCAGCTGTTCCTGGAAGACGCCCCCGGCATCATCGAAGGGCTGGAAGCGGCCGCCAGCAATCGCGACAGCA
TGCATCTGCGCGATCTGGCCCACACCCTGAAGTCCTCCAGCGCCAACGTCGGCGCCCAGGCGCTAGCCAACGCCGCCCGC
CGCATCGAATTGGCCGCACGCACCGGCACCATCGAACGCCCCTCGGTCATGGTCGCCCTGGTCATTGCCGAATACGCCCG
CGCCCGCCTGGCGCTGCTAGGCCAGGTCACCCGTTTGCAAGCCGCCAGTCGCGCTGCGGGCTGA

Upstream 100 bases:

>100_bases
GGCCGCCATGTGGATGAGCGGCGTGGACTTCATCCAGGGCAACCTGGTGCAGACGGTCGGCAAGGAGCTGGACTTCGACT
TCACGAACGCGGTGCTCTGA

Downstream 100 bases:

>100_bases
TTGGCGGGTTAGCCGTGTGGCTGAGTGGCGCGGCTCTCTCGCAAGATCGCAGCGTCGTCGTTCTTCAATCGAGCGTCGTG
GTTCTCAACGAGCGCCAACC

Product: two-component system sensor protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 767; Mature: 766

Protein sequence:

>767_residues
MAARAGGLRGWSTLVALAALVAASAAALQWLGVAGPWRIVTTAASVLALVCGICTAVAAYRASRTLSALTRQLDDTNTAQ
ARMQQELQRHGELEQELLRAKQAAEAASLAKGEFLATMSHEIRTPLNGIIPMLELISRGQLSLDQRDMLQTATGSSLQLL
RIVDDILDYSKLEANKLELEITTFNLRELLDGVAQLMQRTAEGKQLRLGLDIEPSVRLLVRGDPIRLRQVLGNLIGNAIK
FTERGSVDIQLRRLGETRAQHLLRFQVRDTGIGIAADQQARLFRSFAQADASTTRLYGGTGLGLAICKRIIDLMGGRIGV
ESEPGRGATFWFEIPLLKVIGDLQQTTGADAARVMVISSDARLSQRLKRLLDSWGISHVLMETTQEALERVRRHTDDEGF
RCVIADHETLRYSARAVHRALARPEDLSGSRLIWLYGDEPVPDELQANATLVPRQSPDETLRALVLPPEPKPSVAPTLAS
AMIPEPLPPPVAHARDVRILLVEDNPVNLLVAQKLLGVLGFEADTATDGEAALSSMESTRYDMVFMDCQMPVLDGYAATR
RWRAMETESGGRPIPIVAMTANAMAGDRERCLAAGMDDYLSKPVAREQLNACLQRWLPRQALLPGPSTGAPSADAESAAS
AAQARALPILESGVIDELYEVAGADTIRILQLFLEDAPGIIEGLEAAASNRDSMHLRDLAHTLKSSSANVGAQALANAAR
RIELAARTGTIERPSVMVALVIAEYARARLALLGQVTRLQAASRAAG

Sequences:

>Translated_767_residues
MAARAGGLRGWSTLVALAALVAASAAALQWLGVAGPWRIVTTAASVLALVCGICTAVAAYRASRTLSALTRQLDDTNTAQ
ARMQQELQRHGELEQELLRAKQAAEAASLAKGEFLATMSHEIRTPLNGIIPMLELISRGQLSLDQRDMLQTATGSSLQLL
RIVDDILDYSKLEANKLELEITTFNLRELLDGVAQLMQRTAEGKQLRLGLDIEPSVRLLVRGDPIRLRQVLGNLIGNAIK
FTERGSVDIQLRRLGETRAQHLLRFQVRDTGIGIAADQQARLFRSFAQADASTTRLYGGTGLGLAICKRIIDLMGGRIGV
ESEPGRGATFWFEIPLLKVIGDLQQTTGADAARVMVISSDARLSQRLKRLLDSWGISHVLMETTQEALERVRRHTDDEGF
RCVIADHETLRYSARAVHRALARPEDLSGSRLIWLYGDEPVPDELQANATLVPRQSPDETLRALVLPPEPKPSVAPTLAS
AMIPEPLPPPVAHARDVRILLVEDNPVNLLVAQKLLGVLGFEADTATDGEAALSSMESTRYDMVFMDCQMPVLDGYAATR
RWRAMETESGGRPIPIVAMTANAMAGDRERCLAAGMDDYLSKPVAREQLNACLQRWLPRQALLPGPSTGAPSADAESAAS
AAQARALPILESGVIDELYEVAGADTIRILQLFLEDAPGIIEGLEAAASNRDSMHLRDLAHTLKSSSANVGAQALANAAR
RIELAARTGTIERPSVMVALVIAEYARARLALLGQVTRLQAASRAAG
>Mature_766_residues
AARAGGLRGWSTLVALAALVAASAAALQWLGVAGPWRIVTTAASVLALVCGICTAVAAYRASRTLSALTRQLDDTNTAQA
RMQQELQRHGELEQELLRAKQAAEAASLAKGEFLATMSHEIRTPLNGIIPMLELISRGQLSLDQRDMLQTATGSSLQLLR
IVDDILDYSKLEANKLELEITTFNLRELLDGVAQLMQRTAEGKQLRLGLDIEPSVRLLVRGDPIRLRQVLGNLIGNAIKF
TERGSVDIQLRRLGETRAQHLLRFQVRDTGIGIAADQQARLFRSFAQADASTTRLYGGTGLGLAICKRIIDLMGGRIGVE
SEPGRGATFWFEIPLLKVIGDLQQTTGADAARVMVISSDARLSQRLKRLLDSWGISHVLMETTQEALERVRRHTDDEGFR
CVIADHETLRYSARAVHRALARPEDLSGSRLIWLYGDEPVPDELQANATLVPRQSPDETLRALVLPPEPKPSVAPTLASA
MIPEPLPPPVAHARDVRILLVEDNPVNLLVAQKLLGVLGFEADTATDGEAALSSMESTRYDMVFMDCQMPVLDGYAATRR
WRAMETESGGRPIPIVAMTANAMAGDRERCLAAGMDDYLSKPVAREQLNACLQRWLPRQALLPGPSTGAPSADAESAASA
AQARALPILESGVIDELYEVAGADTIRILQLFLEDAPGIIEGLEAAASNRDSMHLRDLAHTLKSSSANVGAQALANAARR
IELAARTGTIERPSVMVALVIAEYARARLALLGQVTRLQAASRAAG

Specific function: Forms part of a two-component regulatory system gacA/gacS(lemA). May be involved in lesion formation, swarming and in the production of extracellular protease, syringomycin and N- acyl-L-homoserine lactone (acyl-HSL). Required for pathogenicity on bean [H

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1789149, Length=648, Percent_Identity=33.641975308642, Blast_Score=297, Evalue=2e-81,
Organism=Escherichia coli, GI145693157, Length=538, Percent_Identity=34.9442379182156, Blast_Score=266, Evalue=5e-72,
Organism=Escherichia coli, GI87081816, Length=404, Percent_Identity=33.1683168316832, Blast_Score=157, Evalue=2e-39,
Organism=Escherichia coli, GI1788713, Length=240, Percent_Identity=38.3333333333333, Blast_Score=149, Evalue=5e-37,
Organism=Escherichia coli, GI48994928, Length=239, Percent_Identity=38.0753138075314, Blast_Score=148, Evalue=1e-36,
Organism=Escherichia coli, GI1786783, Length=305, Percent_Identity=28.5245901639344, Blast_Score=101, Evalue=2e-22,
Organism=Escherichia coli, GI1786600, Length=245, Percent_Identity=31.8367346938775, Blast_Score=97, Evalue=5e-21,
Organism=Escherichia coli, GI1788393, Length=258, Percent_Identity=29.4573643410853, Blast_Score=95, Evalue=2e-20,
Organism=Escherichia coli, GI1790436, Length=247, Percent_Identity=28.7449392712551, Blast_Score=86, Evalue=9e-18,
Organism=Escherichia coli, GI87082128, Length=229, Percent_Identity=26.6375545851528, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI1786912, Length=243, Percent_Identity=27.9835390946502, Blast_Score=82, Evalue=2e-16,
Organism=Escherichia coli, GI1788549, Length=240, Percent_Identity=28.3333333333333, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1787894, Length=241, Percent_Identity=29.045643153527, Blast_Score=74, Evalue=5e-14,
Organism=Escherichia coli, GI1790346, Length=286, Percent_Identity=28.6713286713287, Blast_Score=68, Evalue=2e-12,
Organism=Escherichia coli, GI1788550, Length=125, Percent_Identity=32.8, Blast_Score=66, Evalue=1e-11,
Organism=Escherichia coli, GI1789808, Length=249, Percent_Identity=26.1044176706827, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1789402, Length=121, Percent_Identity=34.7107438016529, Blast_Score=62, Evalue=1e-10,
Organism=Saccharomyces cerevisiae, GI6322000, Length=165, Percent_Identity=35.1515151515151, Blast_Score=95, Evalue=5e-20,
Organism=Saccharomyces cerevisiae, GI6322044, Length=126, Percent_Identity=38.0952380952381, Blast_Score=86, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6323034, Length=184, Percent_Identity=26.6304347826087, Blast_Score=74, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011006
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR008207
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA; PF01627 Hpt; PF00072 Response_reg [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 82973; Mature: 82842

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: PS50894 HPT ; PS50110 RESPONSE_REGULATORY ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAARAGGLRGWSTLVALAALVAASAAALQWLGVAGPWRIVTTAASVLALVCGICTAVAAY
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
RASRTLSALTRQLDDTNTAQARMQQELQRHGELEQELLRAKQAAEAASLAKGEFLATMSH
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
EIRTPLNGIIPMLELISRGQLSLDQRDMLQTATGSSLQLLRIVDDILDYSKLEANKLELE
HHHCCHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEEEE
ITTFNLRELLDGVAQLMQRTAEGKQLRLGLDIEPSVRLLVRGDPIRLRQVLGNLIGNAIK
EEEHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCEEEEEECCHHHHHHHHHHHHHHHEE
FTERGSVDIQLRRLGETRAQHLLRFQVRDTGIGIAADQQARLFRSFAQADASTTRLYGGT
ECCCCCCEEEHHHHHHHHHHHHHHHEECCCCCCCCCCHHHHHHHHHHHCCCCCEEEECCC
GLGLAICKRIIDLMGGRIGVESEPGRGATFWFEIPLLKVIGDLQQTTGADAARVMVISSD
CHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECHHHHHHHHHHHHCCCCCEEEEEEECC
ARLSQRLKRLLDSWGISHVLMETTQEALERVRRHTDDEGFRCVIADHETLRYSARAVHRA
HHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHH
LARPEDLSGSRLIWLYGDEPVPDELQANATLVPRQSPDETLRALVLPPEPKPSVAPTLAS
HCCCCCCCCCEEEEEECCCCCCHHHCCCCEECCCCCCCHHHEEEEECCCCCCCCHHHHHH
AMIPEPLPPPVAHARDVRILLVEDNPVNLLVAQKLLGVLGFEADTATDGEAALSSMESTR
HHCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCC
YDMVFMDCQMPVLDGYAATRRWRAMETESGGRPIPIVAMTANAMAGDRERCLAAGMDDYL
CCEEEEECCCCHHCCHHHHHHHHHCCCCCCCCCEEEEEEECCHHCCCHHHHHHCCCHHHH
SKPVAREQLNACLQRWLPRQALLPGPSTGAPSADAESAASAAQARALPILESGVIDELYE
CCCHHHHHHHHHHHHHCCCHHCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHH
VAGADTIRILQLFLEDAPGIIEGLEAAASNRDSMHLRDLAHTLKSSSANVGAQALANAAR
HHCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHH
RIELAARTGTIERPSVMVALVIAEYARARLALLGQVTRLQAASRAAG
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AARAGGLRGWSTLVALAALVAASAAALQWLGVAGPWRIVTTAASVLALVCGICTAVAAY
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
RASRTLSALTRQLDDTNTAQARMQQELQRHGELEQELLRAKQAAEAASLAKGEFLATMSH
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
EIRTPLNGIIPMLELISRGQLSLDQRDMLQTATGSSLQLLRIVDDILDYSKLEANKLELE
HHHCCHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEEEE
ITTFNLRELLDGVAQLMQRTAEGKQLRLGLDIEPSVRLLVRGDPIRLRQVLGNLIGNAIK
EEEHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCEEEEEECCHHHHHHHHHHHHHHHEE
FTERGSVDIQLRRLGETRAQHLLRFQVRDTGIGIAADQQARLFRSFAQADASTTRLYGGT
ECCCCCCEEEHHHHHHHHHHHHHHHEECCCCCCCCCCHHHHHHHHHHHCCCCCEEEECCC
GLGLAICKRIIDLMGGRIGVESEPGRGATFWFEIPLLKVIGDLQQTTGADAARVMVISSD
CHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECHHHHHHHHHHHHCCCCCEEEEEEECC
ARLSQRLKRLLDSWGISHVLMETTQEALERVRRHTDDEGFRCVIADHETLRYSARAVHRA
HHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHH
LARPEDLSGSRLIWLYGDEPVPDELQANATLVPRQSPDETLRALVLPPEPKPSVAPTLAS
HCCCCCCCCCEEEEEECCCCCCHHHCCCCEECCCCCCCHHHEEEEECCCCCCCCHHHHHH
AMIPEPLPPPVAHARDVRILLVEDNPVNLLVAQKLLGVLGFEADTATDGEAALSSMESTR
HHCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCC
YDMVFMDCQMPVLDGYAATRRWRAMETESGGRPIPIVAMTANAMAGDRERCLAAGMDDYL
CCEEEEECCCCHHCCHHHHHHHHHCCCCCCCCCEEEEEEECCHHCCCHHHHHHCCCHHHH
SKPVAREQLNACLQRWLPRQALLPGPSTGAPSADAESAASAAQARALPILESGVIDELYE
CCCHHHHHHHHHHHHHCCCHHCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHH
VAGADTIRILQLFLEDAPGIIEGLEAAASNRDSMHLRDLAHTLKSSSANVGAQALANAAR
HHCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHH
RIELAARTGTIERPSVMVALVIAEYARARLALLGQVTRLQAASRAAG
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1314807 [H]